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Showing: 1 - 9 of 9 results

  1. Lentiviral Prep Service

    Type
    Collection
    ...73178 Human sgRNA library Brunello in lentiGuide-Puro Human sgRNA library in backbone lentiGuide-Puro targeting...Doench , Root 73179 Human gRNA pooled library in lentiCRISPRv2 Human sgRNA library in backbone lentiCRISPRv2...Puromycin Doench , Root Human activation pooled libraries 92379 Human CRISPRa sgRNA library Calabrese in backbone...knockout pooled libraries 73633 Mouse sgRNA library Brie in lentiGuide-Puro Mouse sgRNA library in backbone...Doench , Root Human inactivation pooled libraries 92385 Human CRISPR inhibition library Dolcetto in XPR...Pooled Libraries . ID Name Description Genes/Insert Selection PI Human knockout pooled libraries 73178 ...backbone XPR_502 (P65 HSF), Set A Human CRISPRa sgRNA activation library in backbone XPR_502 (P65 HSF) targeting...

  2. CRISPR Pooled gRNA Libraries

    Type
    Collection
    ...Knockout Human Parts 3rd 3 59,576 Human Whole Genome sgRNA iBAR Library 140633 Knockout Human Wei 3rd ...Lab Human messenger-RBP sgRNA Library 168791 Knockout Human Turner 3rd 10 8,260 Two plasmid human activity-optimized...Knockout Human Lu 3rd 10 8,205 Bassik Human CRISPR Knockout Library 101926 — 101934 Knockout Human Bassik...103,269 Human CRISPR Knockout Library 1000000132 Knockout Human X.S. Liu 3rd 10 185,634 Human Epigenetic Knockout...plasmid) Knockout Human Zhang 3rd 6 123,411 Human genome-wide library v1 69763 Knockout Human Wu 3rd 4 77,406...,406 Human improved genome-wide library v1 67989 Knockout Human Yusa 3rd 5 90,709 Human Interferon-Stimulated...Knockout Library 123334 Knockout Human Vakoc 3rd 6 8,658 Human Genome-Wide Reduced Double-gRNA Library 137999...

  3. Zhang Lab CRISPR Page

    Type
    Collection
    ...Pooled genome-wide human SAM library SAM can be combined with a human genome-wide library to activate all...top GeCKO Screening Library GeCKO are pooled lentiviral CRISPR libraries (both human and mouse) for performing...described here. #60224 - AAV:ITR-U6-sgRNA(Kras)-U6-sgRNA(p53)-U6-sgRNA(Lkb1)-pEFS-Rluc-2A-Cre-shortPA-KrasG12D_HDRdonor-ITR...contains a single cassette expressing human codon-optimized SpCas9; sgRNA can be co-transfected as PCR amplicons...screens. The libraries are available in two formats: 1 vector system - lentiCRISPR - sgRNA and SpCas9 together...lentiCas9-Blast and lentiGuide-Puro (sgRNA) Browse the Addgene GeCKO Library CRISPR page for more information...has been human codon-optimized, and packages both the SaCas9 gene and its single guide RNA (sgRNA) into ...

  4. CRISPR References and Information

    Type
    Collection
    ...mouse, rat, and human. CCTop - CRISPR/Cas9 target online predictor Identifies candidate sgRNA target sites... Sabatini and Lander gRNA cloning into pLX-sgRNA pLX-sgRNA PDF 125.9 KB Sontheimer gRNA design and cloning...CMV-EGFP Kits are also available (mouse or human libraries) PDF 269.2 KB...as an optional input to assess HDR frequency. An sgRNA sequence (without PAM sequence) can be provided,... can identify positively or negatively selected sgRNAs, genes, or pathways. CrispyCrunch CrispyCrunch ...Editing Toolkit) (Link opens in a new window) is a library of various python functions for the purpose of ...batch processing for designing genome-wide gRNA libraries. PubMed PMID 28348817 . CRISPOR This tool helps...

  5. CRISPR Plasmids - Prime Edit

    Type
    Collection
    ...nicking sgRNA BbsI No Vermilion Norbert Perrimon 164423 pHSG1C3 Mammalian U6 pegRNA + nicking sgRNA BbsI ... RNA-H1-nick sgRNA-mCherry Mammalian, AAV hU6 + H1 pegRNA + nicking sgRNA No mCherry Hyongbum ...HindIII No Tilmann Weber 172717 pnsgRNA Bacteria J23119 (BBa_J23119) nicking sgRNA No Tilmann Weber 173220 pPBT-peRNA_GG-Puro...Purify Tag Visualize dCas9-FokI Screen Pooled Libraries gRNAs Empty gRNA Vectors gRNAs By Species Mammalian...mutations in RT enzyme PE3 - PE2 plus additional sgRNA PE4 - PE2 plus MLH1 (a protein component of MMR)...optimized PE2 enzyme containing RT optimized for human codons, additional nuclear localization signals,...the type of gRNA (e.g., pegRNA, epegRNA, nicking sgRNA), cloning enzyme, selectable marker, and whether...

  6. CRISPR Guide

    Type
    Collection
    ... lentiviral pooled libraries . Browse Libraries: CRISPR Pooled Libraries Cas9 Fusion Tools dCas9 cannot...lentiviral CRISPR libraries. Figure 8: Overview of genome-wide screening using CRISPR libraries What Are Pooled...Pooled Lentiviral CRISPR Libraries? Pooled lentiviral CRISPR libraries are a heterogeneous population of..., so CRISPR libraries contain thousands of unique gRNAs. gRNA design for CRISPR libraries is usually optimized...activation and repression libraries target promoter or enhancer regions. As each library is unique, be sure ...sure to check the library information and original publication to see if a library is suitable for your experiment...CRISPR libraries have been developed for inhibition using dCas9. How Do You Use a CRISPR Library? CRISPR...

  7. CRISPR Plasmids - Empty gRNA Vectors

    Type
    Collection
    ...pgRNA-humanized 44248 Mammalian/Lentiviral BstXI + XhoI none S. pyogenes Puro, mCherrry Qi pLX-sgRNA 50662...pyogenes Joung sgRNA with U6 promoter 48962 Worm HindIII none S. pyogenes de Bono sgRNA with rpr-1 promoter...SpBsmBI sgRNA Hygro 62205 Mammalian/Lentiviral BsmBI none S. pyogenes Hygro Maehr pLenti SpBsmBI sgRNA Puro...dCas9-VP64 along with 1-4 sgRNAs expressed from independent promoters. sgRNAs are cloned into separate ... and allow for expression of more sgRNAs in a single vector. sgRNAs are expressed as polycistronic glycine...pLenti-sgRNA-Lib 53121 Mammalian/Lentiviral BsmBI none S. pyogenes ccdB Wei pLKO5.sgRNA.EFS.PAC 57825 ...Church LRG (Lenti_sgRNA_EFS_GFP) 65656 Mammalian/Lentiviral LIC none S. pyogenes GFP Vakoc U6>sgRNA(F+E) 59986...

  8. CRISPR Plasmids - Tagging

    Type
    Collection
    ... pFETCh_Donor CEBPB Human FLAG pFETCh_CEBPB PX458_CEBPB_1 PX458_CEBPB_2 AHR Human FLAG pFETCh_AHR PX458...AHR_1 PX458_AHR_2 ARID4B Human FLAG pFETCh_ARID4B PX458_ARID4B_1 BCL6_iso1 Human FLAG pFETCh_BCL6_iso1 PX458..._BCL6_iso1_2 CEBPG Human FLAG pFETCh_CEBPG PX458_CEBPG_1 PX458_CEBPG_2 RAD21 Human FLAG pFETCh_RAD21 PX458...PX458_RAD21 GABPA Human FLAG pFETCh_GABPA PX458_GABPA_1 PX458_GABPA_2 GABPB1_iso2 Human FLAG pFETCh_GABPB1...iso2 PX458_GABPB1_iso2_1 ATF1 Human FLAG pFETCh_ATF1 PX458_ATF1 CEBPA Human FLAG pFETCh_CEBPA PX458_CEBPA...PX458_CEBPA_2 CREB1 Human FLAG pFETCh_CREB1 PX458_CREB_1 PX458_CREB_2 TGIF2 Human FLAG pFETCh_TGIF2 PX458...PX458_TGIF2_2 SSRP1 Human FLAG pFETCh_SSRP1 PX458_SSRP1_1 PX458_SSRP1_2 ZNF146 Human FLAG pFETCh_ZNF146...

  9. CRISPR History and Development for Genome Engineering

    Type
    Collection
    ...editing. These edits can include mutations seen in human patients, protein tags, or loxP/FRT sites, among...especially amenable to genetic screens. Pooled gRNA libraries can be used to identify genes that are important...important to a given phenotype. Current libraries are available for gene knockout, as well as transcriptional...germline editing, with the International Summit of Human Gene Editing convened in 2015 to discuss the ethics...plasmids for the following models: Mammalian systems (Human, mouse, and rat) Bacteria ( E. coli , Streptococcus...2013. Dynamic imaging of genomic loci in living human cells by an optimized CRISPR/Cas system. Cell . ... Approach for Mapping Multiprotein Complexes in Human Cells. Cell Rep . 13(3):621-33. PMID: 26456817 Fu...
Showing: 1 - 9 of 9 results