...sonication to break DNA up into fragments of 300-1000 bps in length. Note: This sonication process may... immune response to it. Once the immune response has developed, the antibodies are collected, tested against...epitopes on constant regions of other antibodies. This has useful research applications, and is covered in the...exist as monomers. Each antibody class therefore has a valency, or number of binding sites available per...of antibodies multiplied by two, as each antibody has two binding sites. Isotype Description Configuration... must be able to detect if and where an antibody has bound. Signaling molecules , proteins that emit a...to avoid confusing data. Check that your machine has appropriate lasers and filters for each fluorophore...

...exctinction coefficient and quantum yield divided by 1000. pKa is the pH at which fluorescence intensity is...Addgene has assembled a collection of empty plasmid backbones with different fluorescent tags for you... that result in a diverse set of colors. Addgene has assembled a collection of empty plasmid backbones...

... for Oligomerized Pool ENgineering (OPEN) method has been used to engineer ZFNs that function in zebrafish... The Oligomerized Pool ENgineering (OPEN) method has been used to engineer ZFNs that function in zebrafish... Zinc Finger Consortium: OPEN Reagents (Kit # 1000000013 ) Depositing Labs: Keith Joung This kit consists...publication was a gift from Keith Joung (Addgene kit # 1000000013)" For your Reference section: Oligomerized pool...

...respectfully). This next generation architecture has been shown to increase mutation induction when using...after the repeat domain, and C-terminal SV40 NLS and HA tags. Plasmid 47389 also contains the VP64 domain...double-selection of transfected cells, the system has successfully been employed for deletion mutagenesis...Sangamo)-FokI-ELD-Destination Golden Gate TALEN 2.0 1000000030 Yamamoto Lab TALEN Construction and Evaluation...

...pCINeo-hFX-HA FXN HA, AU1 CMV Friedreich ataxia Michael Ristow 14977 pCINeo-hFX(I154F)-HA FXN HA, AU1 CMV...pBlue-hFX-HA FXN HA, AU1 T7 Friedreich ataxia Michael Ristow 14988 pBlue-hFX(I154F)-HA FXN HA, AU1 T7 Friedreich...pBlue-hFX(T133A)-HA FXN HA, AU1 T7 Friedreich ataxia Michael Ristow 14990 pBlue-hFX(T133E)-HA FXN HA, AU1 T7 Friedreich...-hFX-HA FXN HA, HIS, AU1 T7 Friedreich ataxia Michael Ristow 14992 pET28(a)-hFX(I154F)-HA FXN HA, HIS,...AA88)-HA FXN HA, HIS, AU1 T7 Friedreich ataxia Michael Ristow 15403 pBlue-hFX(I154F)(AA88)-HA FXN HA, HIS...pBlue-hFX-HA(AscI) FXN HA, AU1 T7 Friedreich ataxia Michael Ristow 15405 pBlue-hFX-HA(NcoI/EcoRI) FXN HA, AU1...Camilli 22563 Flag-HA-UCHL1 UCHL1 HA, FLAG Parkinson's Wade Harper 22594 Flag-HA-OTUD4 OTUD4 HA, FLAG CMV Gordon...

.... Cas9 nickase (Cas9n), a D10A mutant of SpCas9, has one active nuclease domain and one inactivated one... available. SaCas9 (from Staphylococcus aureus ) has a coding sequence ~1 kb shorter than SpCas9 while... SpCas9. FrCas9 (from Faecalibaculum rodentium ) has increased fidelity, and recognizes NNTA, a PAM sequence... create the adenine base editor. The TadA domain has also been further evolved to catalyze cytidine deamination...editing requires more optimization and typically has lower efficiency (< 50%) than base editing (> 90%...modifications to eliminate target gene function. It has also been used extensively to screen for novel genes... those for yeast or drosophila . Although CRISPR has been less widely used in bacteria due to technical...

...purchased at Addgene. Each transcription factor isoform has a unique 24 bp barcode that simplifies identification...ID PI Description MORF library 137000, 192821, 1000000218 Zhang A collection of human transcription factor...

... your gene of interest once its appropriate cDNA has been fused to the MS2 binding sites within this plasmid...expression only at a specific time. This collection has a variety of plasmids whose expression levels have...in response to green light. BIOFAB plasmid set 1000000037 N/A N/A Drew Endy This kit contains plasmids ...