...destination vectors for Voytas Golden Gate-assembly kit. 47875 pTALYM4 Golden Gate TALEN 2.0 48705 pTAL7a Boris...PI FusX TALEN Assembly System Contains 336 pFusX clones allowing for Golden Gate assembly of 15-16 RVD ...step rather than two. Stephen Ekker Golden Gate TALEN 2.0 Golden Gate cloning method. Validated in multiple...compatible with Golden Gate TALEN and TAL Effector Kit 2.0. Stephen Ekker Platinum Gate TALEN Kit Platinum...Platinum TALENs assembled by Golden Gate. Validated in several species. Takashi Yamamoto Additional Plasmids... optimal mammalian expression of Voytas Golden Gate-assembled TALEN, both in microinjected embryos and...plasmids and improve the success rate of Golden Gate assembly. Destination vectors, pcDNA-TAL-NC2 and ...

...name Type PI Description High Complexity Golden Gate Assembly Standards Kit Cloning Eric Cantor Kit for... predict high accuracy junction sets for Golden Gate assembly. MoClo Toolkit Plant Expression Sylvestre...Sylvestre Marillonnet 95 plasmids for cloning and assembling standardized parts to create eukaryotic multigene...

...used with the recently developed Golden Gate cloning method to assemble “multiple DNA fragments in an ordered...Simplified representation of the Voytas/Bogdanove Golden Gate TALEN kit. In this example, the final array is...can be made. Intuitively, one would expect that assembling relatively small, repeat-laden DNA regions into...from the University of Minnesota, arrays can be assembled in a matter of days. In an open access Nucleic...custom arrays consisting of 12-31 repeats can be assembled and inserted into a variety of backbones in just... in August, uses a serial ligation protocol to assemble arrays. Dr. Joung, co-founder of the Zinc Finger...early in 2011 and they described their kit for assembling TAL effector arrays in a 2012 issue of Nature...

... for use in this Golden Gate and Gateway based assembly method: plasmids for assembly of single gRNA vectors...separate vectors and then assembled into one construct using Golden Gate assembly. Gersbach MuLE System Mammalian...expression cassettes can be assembled using Golden Gate cloning or the Gibson Assembly. Chen Boosting CRISPR...which can be assembled into the binary CRISPR/Cas9 vectors in one round of cloning by Golden Gate ligation...sgRNAs Zebrafish Plasmids for assembly of gRNA cassettes using Golden Gate ligation into Tol2-based pGGDestTol2LC...nuclease/nickase expression cassette using the Golden Gate cloning method. A separate accessory pack is ...components are then assembled into a single T-DNA binary vector of choice through Gateway recombination. Qi...

... Labs Yarrowia lipolytica Golden Gate tool kit : Integrative vector assembly of up to three transcription...Toolkit - Borodina Lab Golden GATEway cloning system - Wittbrodt Lab MultiSite Gateway Kit : Creating three-part...deposited tools and resources, or read our guide on assembly standards. Browse Plasmids by Microbe System Plasmids...chromosomes - Endy and Shearwin Labs Ralstonia-GG-Kit : Assembly of Ralstonia solanacearum species complex (Rssc...circuit design in E. coli - Freemont Lab MoClo-YTK : Assembly of single and multi-gene constructs for S. cerevisiae...perform your synbio experiment. Synthetic Biology Assembly Standards Guide Bacterial Expression Systems :...oleaginous yeast Y. lipolytica - Rossignol Lab Yeast Gateway kit : 285 vectors for expression, markers, fusion...

...and more. Also contains pre-assembled genetic circuits such as logic gates and higher level gene networks... Cloning Kit GreenGate Cloning System (Plant) MoClo Modular Cloning Modular PUF Assembly Kit Optogenetics...SynBio Resources SynBio Home Browse All SynBio Assembly Standards SynBio Blog Genome Engineering SynBio...James Collins Tom Ellis Drew Endy Tobias Erb Susan Golden John Gray Jeff Hasty Dieter Jahn Alfonso Jaramillo...Quorum Sensing Ellis Lab GeneGuard Endy Lab Logic Gates , BIOFAB Promoter/BCD Kit , and pOSIP Plasmid Kit...Recombinant Phosphoprotein Kit S. cerevisiae Advanced Gateway Destination Vectors TAL Effectors Zinc Finger Consortium...

...Open Enzyme parts have C/E overhangs for Golden Gate assembly. Plasmids Explore the collection and order...Endy Lab Plasmids Jennifer Molloy Lab Plasmids Golden Gate cloning...

...concentration of 1 μM). Ligate annealed oligos into pX330 using a Golden Gate assembly cloning strategy 3 ...utilized to be able to perform PCR in step 6.3 . Assemble a 20 μl PCR with the following components: 10 ...