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  1. Jaenisch Lab CRISPR Plasmids

    Type
    Collection
    ...pAC2-dual-dCas9VP48-sgExpression : Dual expression construct expressing both dCas9VP48 and sgRNA from ...pAC152-dual-dCas9VP64-sgExpression : Dual expression construct expressing both dCas9VP64 and sgRNA from ...pAC153-dual-dCas9VP96-sgExpression : Dual expression construct expressing both dCas9VP64 and sgRNA from ...pAC154-dual-dCas9VP160-sgExpression : Dual expression construct expressing both dCas9VP64 and sgRNA from...Article . Individual plasmids can be ordered via the links below: Table 1. dCas9 Activators sgRNA Dual Expression...guide RNA (sgRNA). While the dCas9 protein provides the transactivation activity, the sgRNA component ...activators and sgRNAs can be separately transfected (pmax dCas9 activators [48223~48227] + sgRNA expression...

  2. CRISPR Plasmids - Empty gRNA Vectors

    Type
    Collection
    ...Gersbach pUC57-sgRNA expression vector 51132 Mammalian BsaI none S. pyogenes Huang pAC154-dual-dCas9VP160-...pyogenes Joung sgRNA with U6 promoter 48962 Worm HindIII none S. pyogenes de Bono sgRNA with rpr-1 promoter...SpBsmBI sgRNA Hygro 62205 Mammalian/Lentiviral BsmBI none S. pyogenes Hygro Maehr pLenti SpBsmBI sgRNA Puro...pyogenes Fire pGL3-U6-sgRNA-PGK-Puro 51133 Mammalian none S. pyogenes Puro Huang pAC152-dual-dCas9VP64-sgExpression...pAC153-dual-dCas9VP96-sgExpression 48239 Mammalian BbsI yes, activate S. pyogenes Jaenisch pAC2-dual-dCas9VP48...dCas9-VP64 along with 1-4 sgRNAs expressed from independent promoters. sgRNAs are cloned into separate ... and allow for expression of more sgRNAs in a single vector. sgRNAs are expressed as polycistronic glycine...

  3. Zhang Lab CRISPR Page

    Type
    Collection
    ...described here. #60224 - AAV:ITR-U6-sgRNA(Kras)-U6-sgRNA(p53)-U6-sgRNA(Lkb1)-pEFS-Rluc-2A-Cre-shortPA-KrasG12D_HDRdonor-ITR...lentiCRISPR - sgRNA and SpCas9 together 2 vector system - lentiCas9-Blast and lentiGuide-Puro (sgRNA) Browse...has both single- and dual-vector systems that allow the delivery of Cas9 and sgRNA in vivo via adeno-associated...its single guide RNA (sgRNA) into one plasmid (Ran et al., Nature 2015). The dual vector system uses S....CMV-driven SaCas9; U6-driven sgRNA 61593 : PX602; TBG-driven SaCas9; U6-driven sgRNA 61592 : PX600; CMV-driven... SaCas9 sgRNA can be co-transfected as PCR amplicons using a protocol similar to SpCas9 sgRNA PCR (Ran... pAAV-U6::sgRNA(SapI)_hSyn-GFP-KASH-bGH (SpGuide acceptor). This is a AAV plasmid for sgRNA cloning. GFP-KASH...

  4. CRISPR Pooled gRNA Libraries

    Type
    Collection
    ...Knockout Fly Perrimon 3rd 8 6 6 7,956 17,827 59,406 Dual sgRNA CRISPRi Libraries 187246 — 187248 1000000192 ...1000000192 Inhibition Human Weissman 3rd 6 Varies Dual sgRNA CRISPRa Libraries 187249 — 187251 1000000193 Activation...46,354 BARBEKO sgRNA Library 174163 Knockout, Base Editing Human Wei 3rd 3 53,502 sgRNA Pool for Streptococcus...131625 Inhibition E. coli Bikard N/A ∼5 21,417 Bison sgRNA Library 169942 Knockout Human Ebert 3rd 4 2,852 ...3rd 2 37,722 Green monkey (Chlorocebus sabaeus) sgRNA library 178284 Knockout Green Monkey Doench and ...Human Weissman 3rd Varies Varies Human CRISPRi-v2 Dual Guide Libraries 197348 197349 Inhibition Human Voorhees...Knockout Human Parts 3rd 3 59,576 Human Whole Genome sgRNA iBAR Library 140633 Knockout Human Wei 3rd 3 58,...

  5. CRISPR References and Information

    Type
    Collection
    ...for transcriptional activation dCas9 activators sgRNA dual expression: pAC2 , pAC152 , pAC153 , pAC154 ;... Sabatini and Lander gRNA cloning into pLX-sgRNA pLX-sgRNA PDF 125.9 KB Sontheimer gRNA design and cloning...as an optional input to assess HDR frequency. An sgRNA sequence (without PAM sequence) can be provided,... can identify positively or negatively selected sgRNAs, genes, or pathways. CrispyCrunch CrispyCrunch ...CRISPR in 180+ genomes. PubMed PMID: 27380939 . sgRNA Scorer 2.0 From the Church Lab : a tool that identifies... epression, and a ctivation From the Qi Lab , a sgRNA design tool for genome editing, as well as gene ...Cas9 target online predictor Identifies candidate sgRNA target sites by off-target quality. Validated for...

  6. Qi Lab CRISPR Page

    Type
    Collection
    ...dCas9) protein and a customized s ingle g uide RNA (sgRNA) designed with a 20-basepair complementary region...any gene of interest. Co-expression of dCas9 and sgRNA can efficiently block transcription (in bacteria...determined jointly by 20-bp matching region on the sgRNA and a short DNA motif (protospacer adjacent motif...plasmid ( pgRNA_bacteria ) contains a constitutive sgRNA expression cassette on a ColE1 vector with ampicillin...pgRNA_humanized ) contains a murine U6 promoter controlled sgRNA cassette, wherein the GN19 can be custom designed...pSLQ1651-sgTelomere(F+E) Lentiviral vector with sgRNA targeting human telomeres 46913 pMSCV-LTR-dCas9-...pSico-based U6 vector containing murine U6 promoter and sgRNA targeting GFP (NT1) 46917 pU6-sgCXCR4-2 Human pSico-based...

  7. Genomic Deletions in Mammalian Cell Lines

    Type
    Collection
    ...example, sgRNA-A’s protospacer sequence and PAM happen to fall on the bottom (Crick) strand while sgRNA-B’s...primers flanking each sgRNA target site (within 150 - 350 bp) to amplify the sgRNA target site to examine...complement: 1.0 μl sgRNA 24- or 25-mer oligo (100 μM; see step 1.4 ), 1.0 μl sgRNA 24- or 25-mer reverse...composed of a polyclonal population exposed to sgRNA-A and sgRNA-B (see step 5 ). The purpose of the following...delivery of a pair of chimeric single guide RNAs (sgRNAs) to create two double strand breaks (DSBs) at a...posted at the top of the page. CRISPR Design Design sgRNAs manually or using freely available online tools...non-coding elements. For creating a gene knockout, two sgRNA located within exons will enrich even monoallelic...

  8. Cre-lox system

    Type
    Collection
    ...60226 AAV:ITR-U6-sgRNA(backbone)-pEFS-Rluc-2A-Cre-WPRE-hGHpA-ITR Cre, luciferase, and sgRNA expression EFS... Zhang 60229 AAV:ITR-U6-sgRNA(backbone)-pCBh-Cre-WPRE-hGHpA-ITR Cre and sgRNA coexpression Cbh AAV Zhang...ITR-U6-sgRNA(backbone)-hSyn-Cre-2A-EGFP-KASH-WPRE-shortPA-ITR Cre, KASH-tagged EGFP, and sgRNA expression...Mammalian Chenn 26745 pDIRE iCre and FlpO, required for dual RMCE EF-1 alpha Mammalian Zeller 26850 pBF3038 Cre...expression hSyn AAV Zhang 60820 pSECC Cre, Cas9 and sgRNA expression EF-1 alpha Lentiviral Jacks 60877 pMAZe...

  9. Zhang Lab's CRISPR Frequently Asked Questions

    Type
    Collection
    ... plate, co-deliver into the cells with the Cas9/sgRNAs vector (like PX330). For large changes (>100bp ... a typical size (~ 5kb) donor plasmid with Cas9/sgRNAs vector, for one well in 24-well plate. A good example...of a typical size (~5kb) donor plasmid with Cas9/sgRNAs vector, for a one well in a 24-well plate. A good...single-cell colonies, grow them up, then perform individual genotyping (using Sanger sequencing, for example...

  10. Fujii Lab CRISPR Plasmids

    Type
    Collection
    ...Cloning Vector Bbs I ver. 2 An empty sgRNA expression vector. sgRNA sequence can be cloned into BbsI site...Figure 2: enChIP general scheme Fujii Lab Plasmids Individual plasmids can be ordered via the links below: ...

  11. FlyCRISPR

    Type
    Collection
    ...replacement by homologous recombination. For the sgRNA, vector pMB60 allows in vitro production of RNA ...) Pubmed (Link opens in a new window) Article Individual plasmids can be ordered via the links below: ...

  12. CRISPR Guide

    Type
    Collection
    ...systems contain two components: a guide RNA ( gRNA or sgRNA ) and a CRISPR-associated endonuclease (Cas enzyme...applications include editing multiple genes at once; using dual nickases to generate a knockout or gene edit; or...using a prime editing guide RNA ( pegRNA ). Like sgRNA, pegRNA directs the nickase to the target site by...efficiency PE3 - increased efficiency; an additional sgRNA directs Cas9n to a nearby site, driving mismatch...- S trand B reak, a break in both strands of DNA Dual nickase/Double nick A method to decrease off-target...in nature and is also commonly referred to as an sgRNA. gRNA scaffold sequence The sequence within the ... J. A., & Charpentier, E. (2012). A programmable Dual-RNA–Guided DNA endonuclease in adaptive bacterial...

  13. CRISPR History and Development for Genome Engineering

    Type
    Collection
    ..., Doudna JA, Charpentier E. 2012. A programmable dual-RNA-guided DNA endonuclease in adaptive bacterial...labeling of genomic loci with dCas9 and engineered sgRNAs using CRISPRainbow. Nat Biotechnol . . PMID: 27088723...separate the individual crRNAs. (3) The tracrRNA and Cas9 nuclease form a complex with each individual, unique...pre-crRNA). (2) The pre-crRNA is processed into individual crRNAs by a special trans-activating crRNA (tracrRNA...

  14. CRISPR Plasmids - Tagging

    Type
    Collection
    ...an expression cassette for the Cas9-programming sgRNA and HR donors for selectable genome modification...guide RNA transcript and a repair template for an individual tagging event. The kit contains 26 plasmids; ...

  15. Neurodegeneration Plasmid Collection

    Type
    Collection
    ...Walker Jackson 78624 sgRNA(MS2)_Prnp_SAM1 PRNP U6 Dementia Walker Jackson 78625 sgRNA(MS2)_Prnp_SAM2 PRNP...His T5 Parkinson's Suzanne Pfeffer 208392 Lenti-sgRNA-Cre-GpNLuc-sgmATM ATM Ataxia telangiectasia Gaorav...GFP, FKBP CMV ALS Sascha Martens 208875 pFastBac-Dual- GST-TEV-TBK1 TBK1 polH ALS Sascha Martens 209078...TARDBP-Exon1-ko-sgRNA1-SpCas9 TARDBP U6 ALS Rajat Rohatgi 107858 pHBS953 TARDBP-Exon2-ko-sgRNA2-SpCas9 TARDBP...133762 px459-TDP-43 sgRNA1 TARDBP U6 ALS Shawn Ferguson 133763 px459-TDP 43 sgRNA2 TARDBP U6 ALS Shawn...TIA1 His, T7 T7 ALS Thomas Tuschl 106108 px458_2A_GFP_sgRNA_EIF2AK2 EIF2AK2 U6 VWM disease Thomas Tuschl ...Episodic ataxia Xiaolong Wang 159788 pGL3-U6-sgRNA_KCNA1-mcherry KCNA1 U6 Episodic ataxia Xiaolong Wang...
Showing: 1 - 15 of 15 results