...library expresses EGFP for easy visualization via direct fluorescence. Morris Control Lentivirus Browse our ...Purpose PI 17446 pLenti CMV GFP Hygro (656-4) GFP Hygromycin 3rd gen lentiviral eGFP expression vector, CMV...-to-use lentivirus available from Addgene's viral vector packaging service. Browse constructs currently... Viral Vector Packaging Service Lentivirus Browse In-Stock Lentivirus Pooled CRISPR Libraries Cas9 Pooled...Pooled Barcoding Libraries Control Addgene's lentiviruses are made from plasmids that are currently in ...along with 1000 non-targeting controls Puromycin Doench , Root Cas9 Lentivirus Cas9 viruses can be used to... 61422 dCAS9-VP64_GFP dCAS9 (D10A, H840A) none Expresses dCAS9-VP64 activator with 2A GFP Zhang 61425 ...

...Production Titer Quality Control Resources Lentivirus Production Titer Quality Control Resources Overview of...'s viral production methods and quality control for lentivirus and AAV. Viral... later, Cre-dependent GFP expression was detected with direct fluorescence. GFP was not detected in the...subunit (RPP30) as a control for normalization purposes are being used. Quality Control Mycoplasma The 293T...virus preps are titered and subjected to quality control by Addgene before being distributed to customers...production protocols, titering methods, and quality control are described below. AAV Production AAV distributed...probes targeting the ITR elements and an internal control of known titer (protocol modified from Lock et ...

...pMKO.1 puro GFP shRNA Negative control vector for pMKO.1 puro; Contains shRNA against GFP William Hahn... digest) David Root 10879 pLKO.1 ‐ TRC control Negative control vector containing non-hairpin insert David...Hahn 10676 pMKO.1 GFP Derivative of pMKO.1 puro with GFP instead of puromycin resistance gene William Hahn...11578 pSico Cre addition causes EGFP to be recombined out of the construct, activating shRNA expression ...the construct, activating shRNA expression Tyler Jacks 11579 pSicoR Cre addition causes both EGFP and ...David Root 1864 pLKO.1 ‐ scrambled shRNA Negative control vector containing scrambled shRNA David Sabatini...and shRNA to be recombined out of the construct, turning OFF shRNA expression Tyler Jacks 12084 pSicoR PGK...

...hUbC-driven EGFP; can be used for cDNA expression Baltimore 12247 pLVTHM 2nd EF-1a-driven GFP and shRNA ...Plasmids Lentiviral Plasmids Additional Resources: Lentivirus Guide Addgene's Viral Service Virus Biosafety...Plasmids Packaging Plasmids Transfer Plasmids Lentiviruses, a subset of retroviruses, are some of the most...and useful types of viruses used in research. Lentivirus can transduce both dividing and non-dividing ... considerations to evaluate when working with lentivirus: these viruses are based on HIV-1, which may ...Viral production Frequently asked questions about lentivirus use Glossary of commonly used lentiviral terms... and Packaging Plasmids One safety feature of lentivirus used in research is that the components necessary...

...need to generate lentivirus containing the entire library (Figure 8D). This lentivirus will then be used...-occurring Acr (Anti-CRISPR) proteins can help control CRISPR activity. Acr proteins have evolved in phages...LmoCas9 and SpCas9. Acr proteins can be used to control both when and where genome editing happens. CASANOVA...Dominik Niopek’s lab , is a way to optogenetically control genome editing by fusing the anti-CRISPR protein...photosensitive LOV2 domain . Several labs have also controlled Acr activity with endogenous cell-type specific... the HDR pathway can also help. Additionally, constructs with Cas9 fused to proteins that modulate HDR...delivery to cells that are poorly transduced by lentivirus. Non-mammalian CRISPR libraries are also available...