...Resources This comprehensive collection of yeast codon-optimized fluorescent proteins (FPs) in a variety of ...fusions of proteins of interest with yeast codon-optimized fluorescent proteins. These vectors are based...Plasmids for constitutive overexpression of codon-optimized FPs in yeast. Vectors are based on the pDRF1...Link opens in a new window) Sheff MA, Thorn KS. Optimized cassettes for fluorescent protein tagging in Saccharomyces...

...plasmid ( pdCas9_humanized ) contains a human codon optimized dcas9 gene under the control of Murine Stem...-humanized A catalytically inactive, human codon-optimized Cas9 expression plasmid 44247 pdCas9::BFP-humanized...-humanized A catalytically inactive, human codon-optimized Cas9-BFP fusion expression plasmid 44248 pgRNA-humanized...expression plasmid for use with either human codon-optimized Cas9 constructs 44249 pdCas9-bacteria A catalytically...

...below: ID Plasmid 46294 pBS-Hsp70-Cas9 : A codon-optimized Cas9 nuclease under the control of the Drosophila...Drosophila hsp70 promoter. 45945 pHsp70-Cas9 : The codon-optimized Cas9 nuclease under the control of the Drosophila...website contains additional information CRISPR Optimal Target Finder Identifies gRNA target sequences ...

... contain two expression cassettes, a human codon-optimized SpCas9 or SpCas9n, and the single guide RNA...contains a single cassette expressing human codon-optimized SpCas9; sgRNA can be co-transfected as PCR ..., Nature Protocols 2013). Individual human codon-optimized SpCas9 plasmids can be ordered via the links...from S. aureus (SaCas9) that has been human codon-optimized, and packages both the SaCas9 gene and its ... contain two expression cassettes, a human codon-optimized SaCas9 driven by either the ubiquitous cytomegalovirus...contains a single cassette expressing human codon-optimized SaCas9; SaCas9 sgRNA can be co-transfected ...contains a single cassette expressing human codon-optimized SpCas9, driven by the truncated MeCP2 promoter...

...pBF3038 Cre codon optimized for yeast Gal1 Yeast Sandmeyer 26853 pBF3060 Cre codon optimized for yeast ...enables visualization of Cre expression. Optimized Cre: Codon-optimized Cre ( iCre ) is expressed at higher...insulin Zebrafish Stainier 24593 AAV-pgk-Cre codon optimized Cre PGK AAV Aebischer 24704 GFAP-Cre Cre GFAP... Cre with stop codon Entry vector Ko 139546 pENTR-CreStopDeleted Cre without stop codon Entry vector Ko... ways to tightly control Cre expression and to optimize Cre expression once it is induced. Addgene has... mutant TEF3 Yeast Piper 61391 pCS2-Cre.zf1 Cre codon modified for zebrafish CMV Zebrafish Burgess 61570... Ko 139547 pENTR-CreERT2 Cre-ERT2 with stop codon Entry vector Ko 139670 pTFUbiCre Cre Maize Ubiquitin...

... tag, TRX, or V5) Mutations Wild type (wt) Codon-optimized expression (recode) Truncated for making pseudovirus...

...of recoded E.coli (rEcoli XpS 192872 ) and an optimized phosphoserine orthogonal translation system (pSerOTS-C1... recoded strain of E. coli ( 68306 ) with the optimized phosphoserine orthogonal translation system ( ... and a new pSerOTS described in: System-wide optimization of an orthogonal translation system with enhanced...Improved Reagents (ca. 2015) described in: A flexible codon in genomically recoded Escherichia coli permits ...Escherichia coli protein synthesis via partial UAG codon reassignment and release factor 1 deletion. Heinemann...

...inhibit MMR PEmax - optimized PE2 enzyme containing RT optimized for human codons, additional nuclear ...PE2–PE5 systems PE6 - small prime editors with optimized RT and/or Cas9 domains PE7 - addition of RNA-binding...

... Plasmids This plasmid collection contains codon-optimized ORF sequences for human SLCs in Gateway-compatible...

...infectious particle production, including codon optimization, deletion of the C-terminal 18-21 amino acids...

...and mutant clones from watermelon have been codon-optimized for expression in bacteria and cloned into ...

...N-terminally His-tagged TALEN fused to an E. coli codon optimized FokI endonuclease domain. pET-Sangamo-His 40787...C-terminally His-tagged TALEN fused to an E. coli codon optimized FokI endonuclease domain. Return to Top Visualization...His-tagged cyan fluorescent protein variant (CyPet) optimized for FRET measurements with a yellow fluorescent...His-tagged yellow fluorescent protein variant (YPet) optimized for FRET measurements with a cyan fluorescent ...

...version of Firefly luciferase that has been codon optimized for expression in mammalian systems. Other ... Click-beetle red luciferase has also been codon optimized ( CBR2opt ) for mammalian expression systems...luciferase. Transcriptional Element Screening Optimized STARR-seq ( S elf- T ranscribing A ctive R egulatory...

...include consistency between lots and the ability to optimize the antibody’s antigen binding sequence to improve...which circumvents the problem of in-frame amber codons in scFvs A new expression vector facilitating production...

... (Link opens in a new window) designed and codon-optimized the Open Enzymes parts for expression in Escherichia...

...Blast Sp Cas9 Cut Blasticidin Expresses human codon-optimized S. pyogenes Cas9 protein and blasticidin resistance...

...p53 for single plasmid reprogramming Novel codon-optimized mini-intronic plasmid for efficient, inexpensive... Fibroblasts Cardiomyocytes Lentiviral Mouse Optimization of direct fibroblast reprogramming to cardiomyocytes...transcriptionally enriched signaling pathways to optimize the conversion of fibroblasts into neurons. Elife...

... an optimized gRNA for your application (see: Plan Your Experiment ). In addition to an optimized gRNA... frameshift mutations leading to premature stop codons within the open reading frame (ORF) of the targeted...structured motif to enhance pegRNA stability PEmax - optimized PE architecture; increased synergy between PE ...compared to HDR. Prime editing requires more optimization and typically has lower efficiency (< 50%) than.... gRNA design for CRISPR libraries is usually optimized to select for gRNAs with high on-target activity...shifting the ORF and/or creating premature stop codons NHEJ N on- H omologous E nd J oining; A DNA repair...sequence ORF O pen R eading F rame; The translated codons that make up a gene PAM P rotospacer A djacent ...

...Gene Type Wildtype Mutant Partial Sequence Codon-Optimized gRNA Other Tags GFP FP other Flag GST His TEV... Pickrell 131792 pHAGE-TBK1-C-FLAG-HA TBK1 STOP codon CMV ALS Alicia Pickrell 131889 pDONR221_SLC1A3 SLC1A3...