...Additionally, these viruses are able to target a broad range of dividing and non-dividing cell types with ...retroviruses , adenoviruses do not integrate into the host genome. Disadvantages of adenoviruses include transient...

...Operon Bacterial expression of luciferase from broad host range plasmid Attila Karsi 105621 Lenti-luciferase-P2A-Neo...dynamic range, and resistance to photobleaching has made luciferase a common choice in assays ranging from...bioluminescence found in a diverse number of organisms, ranging from bacteria to aquatic animals to insects. Luciferase...of expression. Scientists have developed a wide range of luciferase-based plasmid systems for many different...brighter signal and emission in the near-infrared range, enabling enhanced bioluminescence imaging in vivo...toolkit to assess the proteostasis status in a wide range of experimental systems. LumiLuc and LumiScarlet...

...pTac GFP, Luciferase (LuxCDABE) Attila Karsi Broad host range vectors for expressing luciferase and fluorescent...dissect an individual protein’s function under a wide range of highly controlled conditions in vitro , or, if... suitable for replication in many gram-negative hosts. pCri System Various Plasmids T7/lacO and lacO variants...Various Plasmids Blue/UV , Cyan , Green , Yellow , Orange , Red , Far-Red , Near-Infrared , Large Stokes ...

...invading phages and plasmids. CRISPR loci in microbial hosts contain a combination of CRISPR-associated (Cas)...vectors that can be combined with Cas9 in a wide range of applications. Full references are below. The ... studying the function of genetic elements. To broadly enable the application of Cas9 in vivo and ex vivo...

...(HDR) can generate specific nucleotide changes ranging from a single nucleotide change to large insertions...Another Cas9 alternative is xCas9 , which targets a broad set of PAM sequences, such as NG, GAA, and GAT, ...orthologs described above, Cas12a also expands the range of sites that can be targeted by CRISPR to AT-rich...1262. PMID: 30166441 Evolved Cas9 variants with broad PAM compatibility and high DNA specificity.. 2018...screens in E. coli identify essential genes and phage host factors. 2018. Rousset F, Cui L, Siouve E, Becavin...Commun . Jun 27;9(1):2489. PMID: 29950558 2017 A Broad-Spectrum Inhibitor of CRISPR-Cas9. 2017. Harrington...Alkhnbashi OS, Costa F, Shah SA, Saunders SJ, Barrangou R, Brouns SJ, Charpentier E, Haft DH, Horvath ...