... to top RNA Polymerases Addgene ID Plasmid Gene/insert 165505 pOpen-T7gene1 T7 RNA Polymerase 165516 pOpen-SP6rpol... pOpen-SP6rpol SP6 RNA Polymerase 165576 pOpen-T3gene1 T3 RNA Polymerase Return to top Other Enzymes Addgene... DNA Polymerase I 165533 pOpen-TthpolA Tth DNA polymerase 165534 pOpen-T7gene5 T7 DNA Polymerase (unmodified... Ligases DNA Polymerases Restriction Endonucleases Reverse Transcriptases RNA Polymerases Other Enzymes... of molecular biology, including DNA polymerases, RNA polymerases, DNA ligases, reverse transcriptases... DNA Polymerases Addgene ID Plasmid Gene/insert 165500 pOpen-9N7polA 9 degrees N-7 DNA polymerase 165501...

...synthetic guide RNA in vitro from T7 promotor. 47943 pMB70 : Can be used to express a CRISPR guide RNA in C. elegans...the regulatory sequences of an RNA polymerase III transcribed U6 snRNA. In both vectors, the target site...EGFP fusion. For the sgRNA, vector pMB60 allows in vitro production of RNA from the T7 promoter, while pMB70...vectors for the delivery of the synthetic guide RNA (sgRNA). Each of the Cas9 vectors contains the Cas9 ...CRISPR Cas9 gRNA plasmids from Dr. Mike Boxem's lab. CRISPR...You may also like... CRISPR Guide CRISPR Protocols gRNA Design Tools CRISPR Blog Posts We adapted the S....

...the regulatory sequences of an RNA polymerase III transcribed U6 snRNA. In both vectors, the target site...recombination. For the sgRNA, vector pMB60 allows in vitro production of RNA from the T7 promoter, while pMB70...Genome engineering of Drosophila with the CRISPR RNA-guided Cas9 nuclease. Gratz SJ, Cummings AM, Nguyen...targeting chiRNAs Targeting chiRNAs are easily cloned by annealed oligos into the pU6-BbsI-chiRNA plasmid...pU6-BbsI-chiRNA : Plasmid for expression of chiRNA under the control of the Drosophila snRNA:U6:96Ab promoter...-att). 51026 U6-BbsI-crRNA : Generates crRNA for use in combination with tracrRNA. 51434 pHD-DsRed : vector...Browse CRISPR Cas9 gRNA plasmids for drosophila research from the O'Connor-Giles, Wildonger, and Harrison...

...with a 6x His tag. Requires a strain with T7 RNA polymerase. Amp resistant. More expression vectors for...western blotting or staining, and RNA transcript tags to monitor RNA localization. Using these plasmids...Philippe Cluzel RNA localization: Contains a 2x array of the binding sequence for RNA-binding protein,...Philippe Cluzel RNA localization: Contains a 2x array of the binding sequence for RNA-binding protein ...one of the pB1H2w derivatives as a fusion to RNA polymerase subunit ω. Transcription factor binding to ...see here . pgRNA-bacteria 44251 CRISPR Stanley Qi Expression of customizable guide RNA (gRNA) for bacterial...Tagging If you’d like to know where your protein or RNA ends up when it's expressed, you’ll likely need to...