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Showing: 1 - 11 of 11 results
  1. CRISPR Plasmids - Empty gRNA Vectors

    Type
    Collection
    ... pyogenes mCherry Luikart pDECKO_mCherry 78534 Mammalian hU6 none S. pyogenes Puro, mCherry Guigo BPK3079...Mammalian/Lentiviral BbsI none S. pyogenes Puro, TagBFP Yusa lentiGuide-Puro 52963 Mammalian/Lentiviral BsmBI...Mammalian/Lentiviral BsmBI none S. pyogenes Zhang pL-CRISPR.EFS.GFP 57818 Mammalian/Lentiviral BsmBI yes...Mammalian/Lentiviral BfuAI none S. pyogenes Puro Wolfe pL-CRISPR.EFS.tRFP 57819 Mammalian/Lentiviral BsmBI...BbsI)_CBh-Cas9-T2A-mCherry-H1-(BamHI) 64217 Mammalian BbsI yes, cut S. pyogenes mCherry Kuhn pU6-(BbsI)_...CBh-Cas9-T2A-mcherry-P2A-Ad4E1B 64221 Mammalian see plasmid page yes, cut S. pyogenes mCherry Kuhn pU6-(...pU6-(BbsI)_CBh-Cas9-T2A-mCherry 64324 Mammalian BbsI yes, cut S. pyogenes mCherry Kuhn pEX-A-U6-gRNA 65626...
  2. Cre-lox system

    Type
    Collection
    ... Fuc-T2A-cre mCherry and Cre Lentiviral Luikart 66691 FUG-T2A-cre GFP and Cre Lentiviral Luikart 66692...12238 pLOX-CW-CRE Cre CMV Lentiviral Trono 12265 pHR-CMV-nlsCRE Cre CMV Lentiviral Trono 12493 p259 pCMV-... Luo 27546 pLM-CMV-R-Cre Cre and mCherry coexpression CMV Lentiviral Sadelain 30205 Cre-IRES-PuroR Cre...paavCAG-iCre iCre CAG AAV Kim 55632 pAAV-Ef1a-mCherry-IRES-Cre Cre and mCherry coexpression EF-1 alpha AAV Deisseroth...-iCre copGFP and iCre CB Lentiviral Oka 72257 pCDH-CB-iCre iCre CB Lentiviral Oka 73350 pAdx-CMV-iCre-...84032 pHD066 mCherry-Cre fusion and Venus Elastase Zebrafish Stainier 84033 pHD157 mCherry-Cre fusion and...Mammalian Hirrlinger 107312 AAV-hSyn-mCherry-P2A-Cre-WPRE mCherry and Cre; expressed in neurons hSyn AAV...
  3. SARS-CoV-2 Pseudotyped Virus

    Type
    Collection
    ...IRES-ZsGreen-W - Lentiviral dual reporter vector expressing firefly luciferase and ZsGreen. NL4-3 mCherry Luciferase...Luciferase - Lentiviral dual reporter vector expressing mCherry and firefly luciferase. pLentiEGFPdestablized...2A-MCS-Hygro - Lentiviral vector expressing destabilized EGFP. Lenti-luciferase-P2A-Neo - Lentiviral reporter...examples of each in Addgene's collection: HIV-based lentiviral particles Murine leukemia-based retroviral particles...#141347) is available in standard format and as a Lentiviral prep . ID Plasmid Description Mutations Industry...Plasmids Envelope and Packaging Plasmids Popular Lentiviral Envelope and Packaging Plasmids Popular γ-Retrovirus...neutralizing activity are listed below: pBOBI-FLuc - Lentiviral reporter vector expressing firefly luciferase...
  4. Viral Production

    Type
    Collection
    ...in the absence of Cre. mCherry expression alone was detected. pAAV-Ef1a-mCherry-IRES-Cre was a gift from...for in our reported titers. Lentiviral vectors are titered using a lentiviral droplet digital PCR (ddPCR...AAV vectors collection. AAV Data Hub Lentivirus Production Lentiviral preparations are produced in 293T ...performed on lentiviral preparations that have been stored at -80 °C and thawed. Lentivirus titration is...Pro cells were transduced with either pAAV-Ef1a-mCherry-IRES-Cre (55634-AAVrg) alone at 1.7E6 viral genomes...cells are transduced with serial dilutions of a lentiviral vector, incubated for 72 h, and genomic DNA is...and probes targeting integrated copies of the lentiviral Rev responsive element (RRE) gene and the cellular...
  5. Kazuhiro Oka Lentiviral Vectors

    Type
    Collection
    ...Vectors Lentivirus Oka Lab Lentiviral Vectors Baylor College of Medicine Gene Vector Core Lentiviral and ...Resources Addgene Blog: Your Lentiviral Plasmid FAQs Biosafety Resource Guide Lentiviral Transfer Vectors Adenoviral...deposited a series of lentiviral and adenoviral transfer vectors with Addgene. Lentiviral Transfer Vectors ... from the EF1 promoter pCDH-CMV-mCherry-T2A-Puro 72264 Express mCherry and puromycin resistance gene from...infectious virus. Protocol: Lentivirus Transfer Vector Production Protocol Lentiviral Vector ID Purpose pCDH-EF1...Lentiviral Vectors deposited by Kazuhiro Oka from the Baylor College of Medicine Gene Vector Core Viral...and Adenoviral Vectors You may also like... Lentiviral Guide Adenoviral Guide Viral Vector Plasmids and ...
  6. Lentivirus Plasmids

    Type
    Collection
    ...Blog: Tips for Titering Your Lentiviral Preps Lentiviral Pooled Libraries All Viral Plasmids Packaging Plasmids...researcher safety. Read our Lentiviral Guide for more information about: Lentiviral components 2nd versus 3rd...Glossary of commonly used lentiviral terms Popular Lentiviral Envelope and Packaging Plasmids One safety feature...Reiser Return to Top Popular Lentiviral Transfer Plasmids The lentiviral genome (i.e., the genetic material...resistance. Wiederschain 36084 pLV-mCherry 3rd CMV driven expression of mCherry fusion. See plasmids 36083 and...generation lentiviral systems Viral production Frequently asked questions about lentivirus use Glossary...cell upon infection. When lentivirus is used for research, it is the lentiviral genome that encodes genetic...
  7. Fluorescent Protein Guide: Empty Backbones

    Type
    Collection
    ...Mammalian Expression pLV-mCherry - Mammalian Lentiviral Expression pCS2+8CmCherry - Zebrafish/Xenopus/C....pCS2+8NmCherry - Zebrafish/Xenopus/C.elegans/Sea urchin pET mCherry LIC cloning vector (u-mCherry) - Bacterial...Mammalian Retroviral Expression mCherry 587 610 16 4.5 15 min Monomer pcDNA3 mCherry LIC cloning vector (6B) ... mRFP Tsoulfas Lab Lentiviral Plasmids - Fluorescently tag your gene for lentiviral expression Verkhusha... for C. elegans expression pHT101-mCherry - N- or C-terminal mCherry for C. elegans expression Yeast Thorn...Prone to dimerization pLV-Azurite - Mammalian Lentiviral expression pB18cmBFPAzurite - Bacterial Expression...pCAG-GFP - Mammalian Expression pLV-eGFP - Mammalian Lentiviral Expression Ac5-STABLE1-neo - Insect Expression...
  8. Retrovirus Plasmids

    Type
    Collection
    ...expression Chen 52114 pMSCV-IRES-mCherry FP MSCV A bicistronic IRES-mCherry containing retroviral plasmid...Resources: γ-Retrovirus Guide Biosafety Guide Lentiviral Plasmids AAV Plasmids Viral Vectors 101 eBook...
  9. Qi Lab CRISPR Page

    Type
    Collection
    ...The pgRNA_humanized also contains a CMV-puro-t2A-mCherry expression cassette, useful for selection or fluorescent...CRISPR imaging 51024 pSLQ1651-sgTelomere(F+E) Lentiviral vector with sgRNA targeting human telomeres 46913...dCas9 fused to N 51025 pSLQ1661-sgMUC4-E3(F+E) Lentiviral vector that contains sgRNA targeting repetitive...
  10. Neurodegeneration Plasmid Collection

    Type
    Collection
    ...FUGW-APPwt-T2A-mCherry APP mCherry UbC Alzheimer's Ronald Hart 190804 FUGW-APPSwe-T2A-mCherry APP mCherry UbC Alzheimer's...-T2A-mCherry APP mCherry UbC Alzheimer's Ronald Hart 190807 FUGW-APOE-E2-T2A-mCherry APOE mCherry UbC ...T2A-mCherry APOE mCherry UbC Alzheimer's Ronald Hart 190809 FUGW-APOE-E4-T2A-mCherry APOE mCherry UbC ...115331 pLNCX2-mCherry-CHMP2B CHMP2B mCherry CMV ALS Sanford Simon 115333 pLNCX2-mCherry-CHMP2B-siRNAres...Lazarou 119683 pBMN mCherry-OPTN(D474N) OPTN mCherry ALS Michael Lazarou 119684 pBMN mCherry-OPTN(F178A/D474N...Calamai 196704 mCherry-APP-mGFP APP GFP, mCherry CMV Alzheimer's Martino Calamai 196705 mCherry-APPP1-mGFP... CMV Parkinson's Richard Youle 23956 mCherry-Parkin PRKN mCherry CMV Parkinson's Richard Youle 23966 pTreTight-Htt94Q-CFP...
  11. Fluorescent Protein Guide: Biosensors

    Type
    Collection
    ...expression of fluorescent NADH/NAD+ biosensor Peredox-mCherry in plants In Vivo NADH/NAD+ Biosensing Reveals ... Mitophagy Dual-emission mitophagy biosensor (lentiviral expression of pH sensitive mt-mKeima) Spatiotemporal...MOSAIC Kit from the Cohen laboratory includes 19 lentiviral plasmids for a variety of fluorescent sensors...
Showing: 1 - 11 of 11 results