...nEF CAG E2 regulatory element Tag Fusion tags mCherry HA Non-fusion tags mCitrine EGFP dTomato Activity... pAAV SYN1 HA-hM4D(Gi) hM4D(Gi) - Inhibition HA fusion none 2, 5 Richie 121539 pAAV SYN1 HA-hM3D(Gq) hM3D... Roth 50454 pAAV-hSyn-DIO-HA-hM3D(Gq)-IRES-mCitrine hM3D(Gq) - Activation HA fusion and IRES mCitrine ... Roth 50455 pAAV-hSyn-DIO-HA-hM4D(Gi)-IRES-mCitrine hM4D(Gi) - Inhibition HA fusion and IRES mCitrine ... hM3D(Gq) - Activation HA fusion none 2, 5 Richie 50467 pAAV-CaMKIIa-HA-hM4D(Gi)-IRES-mCitrine hM4D(Gi...* Fishell 50472 pAAV-GFAP-HA-rM3D(Gs)-IRES-mCitrine rM3D(Gs) - Activation HA fusion and IRES mCitrine ...Clear Filters ID Name Receptor and Activity Reporter/Tag Cre requirement Serotype PI 44361 pAAV-hSyn-DIO-hM3D...

...C-terminal 3xHA tag for yeast expression Myc Epitope Tag pKMyc - N-terminal Myc tag for mammalian... 2xFLAG tag in pCS for a variety of systems, including Xenopus HA Epitope Tag pKH3 - N... 3xHA tag for mammalian expression pHAHA - N-terminal double HA tag for mammalian... Backbones Flag Epitope tag pcDNA3 Flag HA - N-terminal Flag-HA for mammalian expression...araBAD p15TV-L - His-tagged bacterial expression vector (see tagged expression vectors from...promoter pCS2 mt-GFP - Create myc-tag, GFP-tag fusions for transcription pCSDest - Easy cloning...Gateway recombination Back to Top Epitope Tag or Fusion Protein Tags and fusion proteins are excellent tools...

... Lab TAP Tagging Protocol 97.2 KB Yamamoto PITCh Tagging System The Takashi Yamamoto lab has deposited...Förstemann Drosophila Cell Tagging System The Förstemann lab has developed a CRISPR tagging technique for use ... CRISPR Protein Tagging CRISPR Plasmids - Protein Tagging Browse... CRISPR tagging methods allow for improved efficiency and throughput over traditional tagging methods....design. How to use CRISPR to tag your gene of interest Mendenhall and Myers Tagging System The Eric Mendenhall...deposited plasmids in this CRISPR-Cas tagging system were tested by tagging transcription factors with FLAG ...Protocol 134.7 KB Mendenhall and Myers Tagging Plasmids Protein Species Tag Donor Plasmid gRNA plasmid gRNA ...

...(RBPs) tagged with fluorescent proteins to visualize targeted genomic loci. CRISPR imaging has numerous... Targeting RNA Editing Other Applications Purify Tag Visualize dCas9-FokI Screen Pooled Libraries gRNAs...dynamics in living cells. The fluorescent CRISPR system has been used for dynamic tracking of repetitive and ...visualized in living cells using a single gRNA that has multiple targeted sequences in close proximity. A...numerous advantages over other imaging techniques, including that it is easy to implement due to the simplicity...

... mammalian) Tags (e.g. 6xHis, Flag, StrepII, HA, Myc, SUMO, GST, MBP, CBP, S-tag, TAP tag, TRX, or V5)...pseudovirus (Ctrunc) ID Plasmid Description Mutations Tags Industry Additional Resources Read Addgene's Blog... proteins in several expression systems. Addgene has partnered with Ginkgo to make these plasmids available...

...Endogenous Gene Tagging in Human Cells on the Addgene Blog. Plasmids for Endogenous Gene Tagging in Human Cells...with endogenously-tagged cellular structures. Addgene ID Plasmid Allen Institute ID Tag Protein Structure...Institute for Cell Science has deposited plasmids to generate fluorescently tagged proteins of key cellular...You May Also Like... CRISPR Guide CRISPR Protein Tagging Fluorescent Protein Guide Subcellular Localization...collection (Link opens in a new window) of fluorescently tagged, human, induced pluripotent stem cell (hiPSC) lines...researchers will be able to generate fluorescently tagged proteins of key cellular structures in human cell...TTN-mEGFP AICSDP-45 mEGFP Titin Sarcomeres (M-line tag) 114413 MYL7-mEGFP AICSDP-46 mEGFP MLC-2a Sarcomeric...

...Florida State University has contributed their comprehensive collection of ORFs tagged with a variety of fluorescent...Davidson lab has contributed the corresponding empty backbone with different fluorescent tags for you to...

...NEB's comparison of SNAP-tag, CLIP-tag, and GFP . Technology SNAP-tag SNAP-tag is based on the human O6...non-cell-permeant proteins. CLIP-tag CLIP-tag is identical in function to SNAP-tag but reacts with benzylcytosine...or localization. ACP and MCP tags ACP- and MCP-tags are small protein tags based on the acyl carrier protein...these tags. Plasmids Addgene ID Plasmid Substrate Feature 101137 pSNAP-tag (T7) Vector SNAP-tag Empty ...Plasmid SNAP-tag Control for cell surface localization 101136 pCLIP-tag (m) Vector CLIP-tag Empty backbone...Plasmid CLIP-tag Control for cell surface localization 101127 pMCP-tag (m) Vector ACP/MCP-tag Empty backbone...and CLIP tag FAQs (PDF, 252 KB) ACP and MCP tags ACP-MCP-Tag plasmids - Substrate source (PDF, 46 KB) Please...

...NanoLuc® with either SNAP‐tag or HaloTag7 tag in which labeling of the self‐labeling tag with a fluorophore ...expression systems. Each type of luciferase has advantages and disadvantages depending on the application. Firefly...and Renilla luciferase are less-than-ideal protein tags due to their large size. NanoLuc® Luciferase is ...biosensor that utilizes Renilla luciferase and a Halo tag to assay mRNA decay in real time. pKC-4.04, pKC-4.06...Lentiviral expression of firefly luciferase with a V5 tag Kevin Janes 83281 pAAV-CAG-FLuc Firefly CAG AAV expression...Mammalian expression of Nanoluc with a N-terminal Myc tag Erich Wanker 124701 pLenti-PGK-Venus-Akaluc (neo)...Lentiviral expression of Nanoluc with a N-terminal Myc tag Erich Wanker 115352 pFL-SV40 Firefly SV40 Mammalian...

...cassette in format: PGK-rtTA-2A-puro; see article for tagged insert options rtTA On Root 11651 pLVUT-tTR-KRAB...controls expression of gene of interest with Strep-Tag and Tet-On 3G transactivator, creating an auto-regulated...in both tetracycline on and off systems. Dox also has good tissue distribution, low toxicity, a known half-life...expression of shRNA; neomycin selection; plasmid 21915 has puromycin selection TetR On Wiederschain 41393 pCW57.1...that is silent in absence of binding tTA or rTA; has high copy E. coli origin None Either Ralser 64238...60495 pSBtet-GP Sleeping Beauty transposon system; has luciferase in cloning site; see article for additional...to make cell lines Tet-On Advanced; Plasmid 26730 has hygro resistance 3rd generation rtTA On Campeau 25434...

...Knock-in tagging - Michel Bagnat lab. Targeting cassettes encoding fluorescent proteins for tagging genes...bearing either amino- or carboxyl-terminal tags, including Myc, HA, Flag, GST and eGFP epitopes. Zebrafish...tropical freshwater fish in the minnow family. It has long been established as a powerful vertebrate model...genes having an ortholog in zebrafish, this species has more recently emerged as a model system for human...Keith Joung Lab CRISPR/Cas9-based conditional mutagenesis in zebrafish - Wenbiao Chen Lab A CRISPR/Cas9...rapidly model potential cancer drivers in vivo. Biotagging toolkit - Tatjana Sauka-Spengler Lab. A genetic...

...the repeat domain, and C-terminal SV40 NLS and HA tags. Plasmid 47389 also contains the VP64 domain (four...of a TALE, along with strong nuclear localisation tags and repress transcription initiation when targeted... plasmids 47388 and 47389 include a Flag epitope tag and an SV40 NLS at the N terminus, a 152-residue ...transfected cells, the system has successfully been employed for deletion mutagenesis and gene targeting in human...respectfully). This next generation architecture has been shown to increase mutation induction when using...

...Visualization and Tagging Purification Controlled Expression Reporter Plasmids Reporter Plasmids Tagging and Visualization...artifacts!), photoactivatable tags for super resolution imaging, epitope tags like His for easy western blotting...Visualization Technique/Tag PI Purpose Empty Backbones with Fluorescent protein tags Various Plasmids Blue...interest in bacteria, add epitope tags to it, and, later, cleave those tags from the purified protein. Protein...recombinant protein with an N-terminal 6x His tag and an MBP tag. Compatible with Gateway cloning. pDEST-periHisMBP...recombinant protein with an N-terminal 6x His tag and an MBP tag. Contains a signal sequence to localize the...IPTG inducible) N terminal GST tag with TEV cleavage site to remove tag Nicola Burgess Brown pGEX expression...

...Recommended Applications Browse Epitope Tag Antibodies IPI has developed recombinant versions of antibodies... Glypican Antibodies Integrin Antibodies Epitope Tag Antibodies Antibody Plasmids Welcome to the Institute...proteins (glypicans and integrins) and common epitope tags, as well as antibody plasmids to create or express...antibodies that recognize common epitope tags to help purify, detect, or localize particular proteins. Browse...His, Biotin, EE, GCN4 and Strep. Please visit our Tags and Other Markers Antibody Collection page to browse...s full catalog of antibodies that target epitope tags and other common cellular proteins. ID Recombinant...

... (CAPTURE.) Tag : Multiple methods make it easier to tag endogenous loci with epitope tags or fluorescent...include mutations seen in human patients, protein tags, or loxP/FRT sites, among others. Homology-directed... not occur in mammalian cells. Purify : Epitope-tagged dCas9 can also be used to purify a genomic locus... EM, Myers RM. 2015. CETCh-seq: CRISPR epitope tagging ChIP-seq of DNA-binding proteins. Genome Res . ...Technological Advances CRISPR technology development has occurred at a truly astonishing pace, with work directed...Directions It’s amazing to see how much progress has been made in just five years, with the skyrocketing...see results! Getting Started with CRISPR Addgene has empowered researchers to further develop the CRISPR...

...pCINeo-hFX-HA FXN HA, AU1 CMV Friedreich ataxia Michael Ristow 14977 pCINeo-hFX(I154F)-HA FXN HA, AU1 CMV...pBlue-hFX-HA FXN HA, AU1 T7 Friedreich ataxia Michael Ristow 14988 pBlue-hFX(I154F)-HA FXN HA, AU1 T7 Friedreich...pBlue-hFX(T133A)-HA FXN HA, AU1 T7 Friedreich ataxia Michael Ristow 14990 pBlue-hFX(T133E)-HA FXN HA, AU1 T7 Friedreich...-hFX-HA FXN HA, HIS, AU1 T7 Friedreich ataxia Michael Ristow 14992 pET28(a)-hFX(I154F)-HA FXN HA, HIS,...AA88)-HA FXN HA, HIS, AU1 T7 Friedreich ataxia Michael Ristow 15403 pBlue-hFX(I154F)(AA88)-HA FXN HA, HIS...pBlue-hFX-HA(AscI) FXN HA, AU1 T7 Friedreich ataxia Michael Ristow 15405 pBlue-hFX-HA(NcoI/EcoRI) FXN HA, AU1...Camilli 22563 Flag-HA-UCHL1 UCHL1 HA, FLAG Parkinson's Wade Harper 22594 Flag-HA-OTUD4 OTUD4 HA, FLAG CMV Gordon...

...DNA. The system can use common tags, like 3xFLAG-tag, PA, and biotin tags, or an anti-Cas9 antibody. The...in mammalian cells include: SunTag system - co-expression of epitope-tagged dCas9 and antibody-activator...acid, turn a promoter on/off, or add small protein tags. Precise Modifications Using Homology Directed Repair...genetic modification ). Browse Plasmids: Endogenous Tagging CRISPR Base Editing To overcome low HDR efficiency...Loci Using Fluorophores CRISPR imaging has numerous advantages, including the simplicity of gRNA design...also be fused to the gRNA to recruit fluorescently-tagged RNA-binding proteins (RBPs). Multicolor CRISPR ...dCas9s (e.g. S. pyogenes dCas9 and S. aureus dCas9) tagged with different fluorescent proteins or by fusing...

...analogs Mammalian TAG Huiwang Ai 160041 pRaGE Pyl TAG GFP Y35TAG PylRS M. mazei Bacterial TAG Lital Alfonta...DiZHSeC Mammalian TAG P. Chen 92047 pCOTS-pyl-GFP(35TAG) PylRS M. mazei Cyanobacterial TAG Lital Alfonta ...Alfonta 160089 pPaGE Pyl TAG FliC T248TAG PylRS M. mazei Bacterial TAG Lital Alfonta 160377 pDule-Mb haloTyrRS... Bacterial TAG Peter Schultz 48696 pANAP AnapRS E. coli fluorescent AA, Anap Mammalian TAG Peter Schultz...coli pBof Mammalian TAG Huiwang Ai 68292 SepOTSλ SepRS9 E. coli Sep Bacterial TAG Jesse Rinehart 71403...Bacterial TAG Dieter Söll 104069 pTECH-chAcK3RS(IPYE) AcK3RS Chimeric Nε-acetyl-L-lysine Bacterial TAG David...Bacterial TAG David Liu 104071 pTECH-MmAcK3RS(IPYE) AcK3RS M. mazei Nε-acetyl-L-lysine Bacterial TAG David...

... Targeting RNA Editing Other Applications Purify Tag Visualize dCas9-FokI Screen Pooled Libraries gRNAs...parasites. Moreover, CRISPR-dCas9 gene regulation has also been established for some of these parasites...

...Plasmid Description Gene/Insert Vector Type Promoter Tags PI Do you have suggestions for other plasmids that...on the ion pore domain it is coupled to. Addgene has two main types of PSAMs: 5HT3 coupled, which are ...