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  1. Zhang Lab CRISPR Page

    Type
    Collection
    ...PX460; SpCas9n (D10A nickase) and single guide RNA 48140 : PX461; SpCas9n-2A-EGFP (D10A nickase) and single...Full references are below. 1. SpCas9 (or SpCas9n, D10A nickase) + single guide RNA: These plasmids contain...-EGFP are also available. 2. SpCas9 (or SpCas9n, D10A nickase) + CRISPR RNA array + tracrRNA: This plasmid...single guide RNA 62987 : PX462; SpCas9n-2A-Puro (D10A nickase) and single guide RNA 48137 : PX165; SpCas9...

  2. Gersbach Lab CRISPR Plasmids

    Type
    Collection
    ...expression plasmids encode the nuclease-null dCas9 (D10A, H840A) with HA and FLAG epitope tags either alone...pcDNA-dCas9 : Expresses inactivated S. pyogenes dCas9 (D10A, H840A) in mammalian cells. 47107 pcDNA-dCas9-VP64...VP64 : Expresses inactivated S. pyogenes dCas9 (D10A, H840A) fused to VP64 transactivator domain in mammalian...

  3. Immunology Research Plasmids and Resources

    Type
    Collection
    ...-ATPase, 1 MGC133040, MGC133041, P112, Rpn2, S1 PSMD10 proteasome (prosome, macropain) 26S subunit, non-ATPase...MGC142065, MGC142067 UBXN1 UBX domain protein 1 2B28, UBXD10 ULBP1 UL16 binding protein 1 RAET1I ULBP2 UL16 ...) and short cytoplasmic domain, (semaphorin) 4A CORD10, FLJ12287, RP35, SEMAB, SEMB SEMA4B sema domain...short cytoplasmic domain, (semaphorin) 4D C9orf164, CD100, FLJ33485, FLJ34282, FLJ39737, FLJ46484, M-sema-...membrane anchor (John Milton Hagen blood group) CD108, CDw108, H-SEMA-K1, H-Sema-L, JMH, MGC126692, MGC126696...avian) ERBB, ERBB1, HER1, PIG61, mENA ENG endoglin CD105, END, FLJ41744, HHT1, ORW, ORW1 EPGN epithelial ...

  4. CRISPR References and Information

    Type
    Collection
    ...array: Cas9 (pX260) or Cas9 D10A (pX334) ; tracrRNA: Cas9 (pX330) or Cas9 D10A (pX335) ; PDF 248.6 KB Zhang...marker); pCFJ90 (pharyngeal co-injection marker); pDD104 (Cre recombinase) PDF 102.5 KB Jaenisch CRISPR ...

  5. Doudna Lab CRISPR Plasmids

    Type
    Collection
    ...Cas9. 39315 pMJ825 : Streptococcus pyogenes Cas9 D10A mutation. 39316 pMJ826 : Streptococcus pyogenes ...Cas9. 39318 pMJ841 : Streptococcus pyogenes Cas9 D10A, H840A. 41091 pHMGWA-Pa14Csy4 : Pseudomonas aeruginosa...

  6. EXPRESs plasmids for human platelets

    Type
    Collection
    ...bio-His 51831 TM9-bio-His 51832 TMED1-bio-His 51852 TMED10-bio-His 51853 TMED2-bio-His 51854 TMED4-bio-His...bio-His 51860 PEAR1-bio-His 51861 LAMP2-bio-His 51862 CD109-bio-His 51863 F2R-bio-His 51864 F2RL3-bio-His 51865...

  7. Lentiviral Prep Service

    Type
    Collection
    ...Resistance Activity PI 61422 dCAS9-VP64_GFP dCAS9 (D10A, H840A) none Expresses dCAS9-VP64 activator with... 2A GFP Zhang 61425 lenti dCAS-VP64_Blast dCAS9 (D10A, N863A) Blasticidin 3rd generation lenti vector ...

  8. Church Lab CRISPR Plasmids

    Type
    Collection
    ...codon-optimized Cas9 expression plasmid 41816 hCas9 D10A A human codon-optimized Cas9 expression plasmid ...

  9. CRISPR Guide

    Type
    Collection
    ... nuclease domains, RuvC and HNH. Cas9 nickase, a D10A mutant of SpCas9, retains one nuclease domain and...domains can be rendered inactive by point mutations (D10A and H840A in SpCas9), resulting in a nuclease dead...

  11. Neurodegeneration Plasmid Collection

    Type
    Collection
    ...Halo_C_allele PFN1 Halo ALS Michael Ward 178118 CHCHD10_Halo_N_allele CHCHD10 Halo ALS Michael Ward 178119 UBQLN2_... SOD1 His T7 ALS Elizabeth Fisher 117704 phSOD1-D101G-N'His SOD1 His T7 ALS Elizabeth Fisher 117705 pmSod1...

  12. Cre-lox system

    Type
    Collection
    ... transcription of Cre lac Bacterial Ekker 47551 pDD104 (Peft-3::Cre) Cre eft-3 C. elegans Goldstein 48201...
Showing: 1 - 12 of 12 results