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  1. CRISPR Plasmids - Purify Genomic Loci

    Type
    Collection
    ...particular gRNA. In the enChIP (engineered DNA-binding molecule-mediated ChIP) system, catalytically inactive...researchers have expanded chromatin immunoprecipitation (ChIP) to allow purification of any genomic sequence specified...3xFLAG-tag, PA, and biotin tags, can be used for enChIP, as well as an anti-Cas9 antibody. Biotin tagging...

  2. Fujii Lab CRISPR Plasmids

    Type
    Collection
    ...Locus-specific ChIP technologies consist of insertional chromatin immunoprecipitation (iChIP) and engineered...developed in 2009 (Hoshino & Fujii), the locus-specific ChIP technologies enable us to purify specific genomic....12573. Epub 2018 Feb 26. PubMed . Locus-specific ChIP combined with NGS analysis reveals genomic regulatory...chromatin immunoprecipitation (enChIP; Fujita & Fujii, 2013). In iChIP, specific genomic regions tagged... Fujii Lab web page . Figure 1: iChIP general scheme Figure 2: enChIP general scheme Fujii Lab Plasmids...3xFLAG-dCas9/pCMV-7.1 enChIP plasmid, expresses 3xFLAG-dCas9 in mammalian cells for enChIP analysis to purify...8xLexA-binding elements/pMD20 iChIP plasmid, LexA-binding elements 48874 3xFNLDD/pCMV-7.1 iChIP plasmid, expresses...

  3. Antibody Guide

    Type
    Collection
    ... Special considerations One type of ChIP assay, called native ChIP, does not use cross-linking and instead...Immunoprecipitation (IP) Chromatin immunoprecipitation (ChIP) Visualization - used to visualize protein location...immunoprecipitation (IP) and chromatin immunoprecipitation (ChIP) allow you to isolate specific proteins or protein...protein from serums. Chromatin immunoprecipitation (ChIP) This variation of IP uses antibodies to capture...strong binding between the protein and DNA. Some ChIP protocols use enzyme digestion instead of sonication... In addition to the control used in standard IP, ChIP should additionally use a beads-only control which...beads) to determine background signal levels. In ChIPseq, an input control will need to be collected before...

  4. Depositor Collections

    Type
    Collection
    ... system - Jaenisch Locus-specific ChIP technologies (iChIP & enChIP) using dCas9 - H. Fujii Disease Research...

  5. CRISPR History and Development for Genome Engineering

    Type
    Collection
    ... and its associated proteins or RNAs through ChIP (enChIP) or biotin-streptavidin pulldown (CAPTURE.) ...Myers RM. 2015. CETCh-seq: CRISPR epitope tagging ChIP-seq of DNA-binding proteins. Genome Res . 25(10)...molecule-mediated chromatin immunoprecipitation (enChIP) using CRISPR. Biochem Biophys Res Commun . 439...

  6. CRISPR Guide

    Type
    Collection
    ...Purify Genomic Regions The enChIP (engineered DNA-binding molecule-mediated ChIP) system, developed by the...the DNA, to create a DSB enChIP En gineered DNA-binding molecule-mediated ChIP , using a tagged-dCas9+gRNA...pairs CRISPR with chromatin immunoprecipitation (ChIP) to allow purification of any genomic sequence specified... R. M. (2015). CETCh-seq: CRISPR epitope tagging ChIP-seq of DNA-binding proteins. Genome Research . 25...molecule-mediated chromatin immunoprecipitation (enChIP) using CRISPR. Biochemical and Biophysical Research...

  7. Trimmer Lab NeuroMab Collection

    Type
    Collection
    ...219444 Anti-KChIP1 [K55/9R] KChIP1 Rat Mouse IgG2a 219445 Anti-KChIP1 K+ channel [K55/59R] KChIP1 K+ channel...190284 Anti-KChIP3 K+ channel [K66/27] KChIP3 K+ channel Rat Mouse IgG2a 190285 Anti-KChIP3 K+ channel ... 206760 KChIP3 K+ channel scFv [K66/27] K66/27 scFv KChIP3 K+ channel Rat Mouse 206761 KChIP3 K+ channel...38 scFv KChIP3 K+ channel Rat Mouse 206762 KChIP3 K+ channel scFv [K66/59] K66/59 scFv KChIP3 K+ channel...Human Mouse IgG2a 177442 Anti-Pan-KChIP K+ channel [K55/82R] Pan-KChIP K+ channel Mouse IgG2a 177443 Anti-CASK...CASK Human Mouse IgG2a 177444 Anti-KChIP2b K+ channel [K60/73R] KChIP2b K+ channel Rat Mouse IgG2a 177445...external) Human Mouse IgG2a 188175 Anti-KChIP2b K+ channel [K60/87R] KChIP2b K+ channel Rat Mouse IgG2a 188176...

  8. CRISPR Plasmids - Bacteria

    Type
    Collection
    ... and its associated proteins, RNA, and DNA. The enCHIP system uses an anti-FLAG antibody to immunoprecipitate...molecule-mediated chromatin immunoprecipitation (enChIP) using CRISPR. Biochem Biophys Res Commun. 2013...

  9. CRISPR Plasmids - Mammalian Expression

    Type
    Collection
    ... and its associated proteins, RNA, and DNA. The enCHIP system uses an anti-FLAG antibody to immunoprecipitate...using streptavidin affinity purification. For both enCHIP and CAPTURE, design your gRNA sequence to direct...

  11. CRISPR Plasmids - Yeast

    Type
    Collection
    ... and its associated proteins, RNA, and DNA. The enCHIP system uses an anti-FLAG antibody to immunoprecipitate...

  13. Genetic Code Expansion

    Type
    Collection
    ...Simon Elsaesser 140020 pAS_4xMma PylT_FLAG-AbKRS-chIPYE chimeric PylRS Chimeric photo-crosslinking-lysine...
Showing: 1 - 13 of 13 results