... Special Collections Fluorescent Protein Guide Fluorescent Protein Plasmids & Resources You May...Avoiding the Dark Side of Fluorescent Protein Fusions with mOX FPs Fluorescent Protein Kits Blog: When is a... variety of fluorescent proteins, as well as over 100 empty backbones. Fluorescent Protein Kits cpFRET...and/or function of your protein of interest by fusing it to a fluorescent protein. FRET Find FRET pairs ...standards to study protein-protein interactions or conformational changes within a protein. Biosensors Monitor...where your protein of interest resides by using a well-characterized fluorescent fusion protein. In Vivo...Use this guide to learn about the applications of fluorescent proteins and to find the plasmids that are...

...Plasmid Collections Fluorescent Proteins Fluorescent Biosensors Fluorescent Proteins: Biosensors Metal ...cyclic AMP) Red fluorescent protein-based cAMP indicator (Pink Flamindo) Red fluorescent protein-based cAMP... AAV Biosensors Fluorescent biosensors are proteins designed so that their fluorescent properties are ...Glucose Green fluorescent glucose indicators for living cells (Glifons) Green Fluorescent Protein-Based Glucose...Lactate Lindoblum green fluorescent biosensor for lactate Green fluorescent protein-based lactate and pyruvate...Pyruvate Pegassos green fluorescent biosensor for pyruvate Green fluorescent protein-based lactate and pyruvate...Robert Campbell pH Red fluorescent pH biosensor mNectarine Red fluorescent protein pH biosensor to detect...

...Special Collections Fluorescent Protein Guide Empty Backbones Fluorescent Protein Guide: Empty Backbones... backbones with different fluorescent tags for you to create fusion proteins with your gene of interest...Backbones More Fluorescent Protein Resources: FRET Biosensors Subcellular localization Michael Davidson Collection... Imaging Blog: Avoiding the Dark Side of Fluorescent Protein Fusions with mOX FPs Blog: When is a Monomer...Monomer not a Monomer? Background Fluorescent proteins are genetically encoded tools that are used extensively...extensively by life scientists. The original green fluorescent protein (GFP) was cloned in 1992 ( Prasher et al....

... Plasmid Collections Fluorescent Proteins Organelle Markers Fluorescent Proteins: Subcellular Localization...marker protein is fused to GFP, you may want to fuse your protein to a red fluorescent protein (see this...structures. You should fuse your protein of interest with a fluorescent protein that has a different emission...your fusion protein and the marker fusion protein using fluorescence microscopy, you can assess whether...Plasmids encoding fluorescent proteins tagged with genes or peptides with known subcellular localization...Localization More Fluorescent Protein Resources: Empty Backbones Allen Institute for Cell Science Plasmid Collection...Collection FRET Biosensors Optogenetics Fluorescent Protein Blog Posts Background General Cytoskeletal...

...Photoconvertible , and Photoswitchable fluorescent proteins Various PIs Fluorescent protein fusion: contains empty vectors...and C-terminal fluorescent protein tags that can be used to create fluorescent protein fusions with your...mEos2 20341 mEos2 (fluorescent protein) Loren Looger Photoactivable Fluorescent Protein Expression: contains...your protein of interest. See an overview of Addgene fluorescent protein resources here . pCyPet-His 14030...cyan fluorescent protein variant (CyPet) optimized for FRET measurements with a yellow fluorescent protein...yellow fluorescent protein variant (YPet) optimized for FRET measurements with a cyan fluorescent protein...systems can be used to measure protein-protein interactions; if two proteins of interest interact in a bacterial...

...encoding Cre-dependent expression of the fluorescent protein EYFP from the CAG promoter Viviana Gradinaru...AAV genome that ubiquitously expresses the fluorescent protein eYFP from the CAG promoter Viviana Gradinaru...hSyn1-eYFP An AAV genome that expresses the fluorescent protein eYFP from the hSyn1 promoter Viviana Gradinaru...

...recruit specific RNA-binding proteins (RBPs) tagged with fluorescent proteins to visualize targeted genomic...compatible with fluorescence microscopy in living cells. Alternatively, gRNAs can be fused to protein-interacting...catalytically inactive Cas9 (dCas9) fused to a fluorescent marker like GFP, researchers have turned dCas9...live cell imaging. Compared to techniques like fluorescence in situ hybridization (FISH), CRISPR imaging... the chromatin dynamics in living cells. The fluorescent CRISPR system has been used for dynamic tracking...requires recruitment of many copies of labeled proteins to the given region. For example, chromosome-specific...RNA Targeting RNA Targeting RNA Editing Other Applications Purify Tag Visualize dCas9-FokI Screen Pooled...

...expression system. Fluorescent Protein Guide : Addgene's extensive fluorescent protein plasmid collection...systems) and therefore supplied as one item. Fluorescent Proteins FRET-based biosensors : E. coli expression...three-part fusion proteins - Planas and Santalucia Labs pCri System : Heterologous protein expression in ...EcoFlex MoClo : Modular cloning for applications like recombinant protein purification and cell-free circuit...Kits for Microbiology : Sets of plasmids for fluorescence, cloning and expression, and synthetic biology...expression - Pertz Lab Bacterial Fluorescence Labeling : Broad-Range Rainbow Vectors - Barbier Lab Cloning and... : 285 vectors for expression, markers, fusion proteins, etc - Lindquist Lab Synthetic Biology BIOFAB ...

...collections. Depositor Collection Types CRISPR Fluorescent Proteins Protein Expression Synthetic Biology Viral Vectors...pooled libraries. Find tools for CRISPR, fluorescent proteins, optogenetics, biosensors, and more. Addgene...Cre-lox TALENs Zinc Fingers Luminescence Fluorescent Proteins Luciferase Optogenetics Chemogenetics Viral.... Click here to view all kits. Kit Types Fluorescent Proteins Cloning Genome Engineering Pathways & ORFs...RESOLUTE Plasmid Collection for Solute Carrier Proteins (SLCs) Structural Genomics Consortium Zinc Finger...resources group plasmids together based on type or application. Browse our guides to find the plasmids and science...

...Alias: CN4496) Expression of SYFP2 (yellow fluorescent protein) plus separate nuclear FLAG epitope in striatal...Alias: CN2610) Expression of SYFP2 (yellow fluorescent protein) in striatal direct pathway medium spiny ...Alias: CN2408) Expression of SYFP2 (yellow fluorescent protein) in striatal and neocortical Sst-Chodl neurons...Alias: CN2787) Expression of SYFP2 (yellow fluorescent protein) in striatal Pvalb+ neurons. Note strong ...Alias: CN3038) Expression of SYFP2 (yellow fluorescent protein) in striatal and lateral septum cholinergic...Alias: CN2700) Expression of mTFP1 (cyan fluorescent protein) in striatal indirect pathway medium spiny...Alias: CN2237) Expression of SYFP2 (cyan fluorescent protein) in striatal indirect pathway medium spiny...

...then purified from the supernatant using Protein A or Protein G affinity columns. Formulation Buffer After...measured and the ratio of the antibody protein content to the total protein content of the sample is determined...and a species-specific fluorescently-labeled secondary antibody. The fluorescence pattern is compared to...secondary antibody. Protein expression is compared to an untransfected control and the protein size is confirmed...by Addgene were developed by the Institute for Protein Innovation (IPI). For these antibodies, IPI will...these antibodies, please visit the Institute for Protein Innovation Antibody Collection webpage. Antibody...concentration and stained with Coomassie blue. Protein band intensities are measured using ImageJ software...

...fused to a fluorescent protein (FP) can help visualize specific genomic loci using fluorescent microscopy...Catalytically dead dCas9 fused to a cytidine deaminase protein becomes a specific base editor that can alter DNA...purify a region of genomic DNA and its associated proteins, RNA, and DNA. The enCHIP system uses an anti-... avoiding known transcription factor and other protein binding sites. ID Plasmid Gene/Insert Promoter ...may also be fused to gRNA sequences to produce fluorescence at a target region, as in the CRISPRainbow kit...using CRISPR, you will need to express both a Cas protein and a target-specific gRNA in the same cell at ...Single plasmids containing both the gRNA and Cas protein act as all-in-one vectors, but their function is...

... DNA repair protein, and is suitable for both cell-permeant and non-cell-permeant proteins. CLIP-tag CLIP-tag...complementary protein function or localization. ACP and MCP tags ACP- and MCP-tags are small protein tags based... labeling cell-surface proteins, and are useful for labeling secreted proteins with disulfide bridges ... Users may still generate their own fluorescent or non-fluorescent CoA substrates by purchasing coenzyme...internal or surface proteins Single constructs are compatible with multiple applications (different fluorophores...ACP or MCP tagged plasmids for visualization of proteins inside cells. Depositor...Information New England Biolabs offers non-toxic protein labeling systems based on SNAP-, CLIP-, ACP-, and...

...done using Protein A, Protein G, or the recombinant Protein A/G, which are bacterial proteins that bind...amounts of protein. Includes: Western blot ELISA Capture - used to isolate specific proteins or protein complexes...quantify, identify, separate, and locate proteins of interest. The protein that an antibody recognizes is called...genetically engineered small proteins derived from ankyrin repeat proteins. These are antibody mimics instead...to the protein of interest. Signal strength is determined by how many antibodies bind the protein of interest...amounts of protein. Western blot Western blots use antibodies to identify denatured proteins separated ...primarily show a protein’s presence or absence. Figure 3: A western blot detecting proteins using the indirect...

...Assays monitoring impedance, fluorescence, mass/charge properties, protein thermal stabilization, and changes...to boost the research focused on Solute carrier proteins (SLCs). Browse the SLC plasmid collection. Depositor...Plasmids Pooled Libraries Feedback Solute carrier proteins (SLCs) are the largest family of transporters ...contribute to assess ligand action/engagement. Protein production is used for assays and to generate antibody... libraries target the solute carrier family of proteins. Library ID PI Description Human SLC Activation... CRISPR activation library targeting human SLC proteins. Human SLC Knockout Library 132552 Superti-Furga...Superti-Furga CRISPR knockout library targeting human SLC proteins. Mouse SLC Knockout Library 141436 Superti-Furga...

...marks. Visualize : Fluorescently labeled dCas9 or gRNAs that bind fluorescent proteins can be used to image...re-exposure. More specifically, specialized Cas proteins snip foreign DNA into small fragments approximately...stretches of DNA known as CRISPR arrays. Separate Cas proteins then express and process the CRISPR loci to generate... varying PAM sequences and number/types of Cas proteins. Makarova et al. ’s classification defines 5 types... to DNA. Others inhibit the recruitment of Cas proteins to the CRISPR-Cas complex. Recently, researchers... can include mutations seen in human patients, protein tags, or loxP/FRT sites, among others. Homology-directed...used to purify a genomic locus and its associated proteins or RNAs through ChIP (enChIP) or biotin-streptavidin...

...catalytically inactive dCas9 to a fluorescent marker like green fluorescent protein (GFP), creating a customizable...tagged with different fluorescent proteins or by fusing the gRNAs to orthogonal protein-interacting RNA aptamers...specificity and applicability of base editing through protein engineering and protein delivery. Nature...naturally-occurring Acr (Anti-CRISPR) proteins can help control CRISPR activity. Acr proteins have evolved in phages ...; or inducing degradation of the Cas9 protein. Most Acr proteins are specific to a particular type of ...and antibody-activator effector proteins; long-term imaging of proteins in living cells (Figure 9B) dCas9...fused to the gRNA to recruit fluorescently-tagged RNA-binding proteins (RBPs). Multicolor CRISPR imaging can...

... and CRISPR tools Fluorescent Proteins from the Michael Davidson collection Protein Labeling from New ... Structural Genomics Consortium Solute Carrier Proteins (SLCs) from the RESOLUTE collection AAV Packaging...antibodies are produced in-house and undergo application-specific validation and quality control by Addgene... Recombinant Antibody Reactivity Recommended Applications PI Don’t See What You’re Looking For? Please...

...requiring only a nuclease-deficient Cas9 (dCas9) protein and a customized s ingle g uide RNA (sgRNA) designed...expression cassette, useful for selection or fluorescent gating of transiently transfected cells. Co-expression...could cause up to 2~3-fold repress on targeted fluorescent genes. These plasmids are described in: Repurposing...compared to several recently published works on applying the wild-type CRISPR system for genome mutagenesis...

...co-immunoprecipitation to assess protein-protein interactions. NanoLuc® complementation : Protein-protein assays based on... Plasmid Collection You may also like... Fluorescent Proteins Blog: Luciferase 101 Blog: Technologies ...-learning approaches. Split-Luciferase and Protein-Protein Interaction Assays Cluc-FKBP , FRB-Nluc : Split...mmunoprecipitation) plasmids : Luminescence-based protein-protein screening method for the systematic and quantitative...NanoLuc® have been used to assess GPCR-G protein interactions , protein dimerization , endosomal escape of ..., Enhanced Nano-lanterns , and Antares : Fluorescent proteins are fused to Renilla luciferase (Nano-lanterns...Firefly luciferase and Renilla luciferase have short protein half-lives, making them useful as transcriptional...