...Addgene's guide to using CRISPR plasmids in your lab, from knockouts to pooled library screening.... cell must be validated experimentally. Browse Plasmids: Double-Strand Break (Cut) Multiplex Genome Engineering...cases, delivering multiple gRNAs using a single plasmid ensures that all gRNAs will be expressed in the...likelihood that any cell containing the CRISPR plasmid will have all desired genomic edits carried out...genetic loci by cloning multiple gRNAs into a single plasmid — though some have achieved targeting in the double...later. Read more about Cas9 multiplexing . Browse Plasmids: Multiplex gRNA Vectors Engineering the Cas9 Endonuclease...specificity and activity are discovered. Browse Plasmids: Single-Strand Break (Nick) , Double-Strand Break...

... Plasmid Collections Pleiades Promoter Plasmids Pleiades Promoter Project The...using the links to view individual plasmid webpages. These plasmids are described in: A regulatory toolbox...Plasmids from the Pleiades Promoter Project - A regulatory toolbox of MiniPromoters to drive selective...human MiniPromoters. Browse the Pleiades Promoter Plasmids using the table below. Additional information ...

... in specific cell types. See our Chemogenetics Plasmids and Chemogenetics Guide pages for more information...retrograde serotype, produced with the AAV retro helper plasmid from the Karpova and Schaffer labs. See the Retrograde...options. Select from a vast catalog of eligible AAV plasmids and we’ll make the viral vector prep you need....

...We provide high quality AAV preps from select plasmids in the repository. Browse our optogenetics AAV...electrical potentials in neurons. See our Optogenetics Plasmids and Optogenetics Guide page for more information... ready-to-use AAV preparations of optogenetics plasmids available in Addgene's collection. Opsin Excitatory...retrograde serotype, produced with the AAV retro helper plasmid from the Karpova and Schaffer labs. See the Retrograde...options. Select from a vast catalog of eligible AAV plasmids and we’ll make the viral vector prep you need....

...Transfer plasmids with sequence from the 5′ to the 3′ ITR exceeding 4.7 kb Transfer plasmids with a cargo...

... BRAIN Armamentarium Project below. Plasmids ID Plasmid Description PI Viral Preps ID Viral Prep Description...underlie behavior and network function. Browse plasmids or viral preps created with support from the BRAIN...

...little variation. Recombinant plasmids - Antibodies can be produced from plasmids, with an antibody’s genetic... genes from specific hybridoma cells into plasmids. Plasmids are then transfected into cells and induced...immune cells, or through the use of recombinant plasmids. Animals - Antibodies produced in animals make...genetic code inserted into the plasmid. This produces the least amount of variation between antibodies, allows...should produce the same antibody clone, or from plasmid-based expression. Monoclonal antibodies are necessary... ready-to-use recombinant antibodies made from plasmids in our collection. View Antibodies...

...preparations were produced with the rAAV2-retro helper plasmid (Addgene #81070) . This serotype permits robust...SYBR green qPCR , confirmation of the transfer plasmid with PCR, purity assessment by silver staining,...Browse all Retrograde AAV *The AAV retro helper plasmid was generated as a part of the Janelia Research...options. Select from a vast catalog of eligible AAV plasmids and we’ll make the viral vector prep you need....

...We provide high quality AAV preps from select plasmids in the repository. Browse our control AAV collection...retrograde serotype, produced with the AAV retro helper plasmid from the Karpova and Schaffer labs. See the Retrograde...options. Select from a vast catalog of eligible AAV plasmids and we’ll make the viral vector prep you need....

... Plasmid Collections Zinc Finger Consortium Zinc Finger ...encoding zinc finger arrays should be cloned into plasmids also available through Addgene--the specific vector...

...NeuroMab/Trimmer Lab Plasmid Collection R-mAb Plasmids scFv Plasmids Nanobody Plasmids Protocols References... Antibody Plasmid Collection NeuroMab Plasmid Collection... the plasmid, followed by the SV40 polyA tail (SV40 pA). Recombinant Monoclonal Antibody Plasmid Collection...following plasmids can be used to produce R-mAbs from transfected mammalian cells. Addgene ID Plasmid Target...partner labs. Learn More scFv Plasmid Collection The following plasmids can be used to produce scFvs from... Pan-GRK Mouse Mouse Nanobody Plasmid Collection The following plasmids can be used to produce nanobodies...research. Find plasmids encoding these R-mAbs here. Antibody...

...? Check out our current collection of Antibody Plasmids , fill out our Suggest an Antibody form, or e-...

...? Check out our current collection of Antibody Plasmids , fill out our Suggest an Antibody form, or e-...

...others to join their cause. Browse the available plasmids from the NETRF-funded investigators listed below...

... on Request service. You can choose a transfer plasmid from our repository and request packaging in one...

...posts , or find CRISPR plasmids for your research. Addgene has CRISPR plasmids for the following models... in the field, and enabling access to multiple plasmids used for various platform applications. For more...

...options. Select from a vast catalog of eligible AAV plasmids and we’ll make the viral vector prep you need....

...Khakh Discontinued Penn Vector Core Viral Vectors (Plasmids still available, other serotypes may be available...available, click on the links for more information. Plasmids are available for all the items listed below. ...pAAV.CMV.Luc.IRES.EGFP.SV40 James M. Wilson AV-9-PV1262 Plasmid not received AV-9-PV1522 100056-AAV9 pAAV.EF1a.....ChR2-YFP.WPRE.hGH Karl Deisseroth AV-9-PV1814 Plasmid not received AV-9-PV2055 105546-AAV9 pENN.AAV.CB7...

...Pre-constructed pairs of TALEN plasmids targeting human genes involved in cancer and epigenetic regulation...

...options. Select from a vast catalog of eligible AAV plasmids and we’ll make the viral vector prep you need....