... Plasmid Collections Cre-lox Plasmids Cre-lox Plasmids Additional...Cre-lox Plasmids Addgene's Blog: Cre-lox Addgene's Blog: FLEx Vectors Cre-containing Plasmids loxP Constructs...loxP plasmids, reporter genes indicate which cells have undergone Cre recombination. These plasmids can...suggestions for other plasmids that should be added to this list? Fill out our Suggest a Plasmid form or e-mail... Cre-lox plasmids for your experiments. Plasmid...alternatives from Gerald Rubin's lab. Cre-containing Plasmids Scientists have developed ways to tightly control... Addgene has a wide variety of Cre recombinase plasmids. Special features include: Inducible Cre: These...

...usage of the pSpCas9(BB) plasmid (pX330) (Addgene plasmid ID 42230). This plasmid allows for the simultaneous...utilized, such as pX458 (Addgene plasmid ID 48138) or pX459 (Addgene plasmid ID 48139), which include GFP ... with a PCR cloning kit) into a plasmid vector. Transform the plasmid into DH5α E. coli cells and plate...Mammalian Cell Lines Other Addgene Resources CRISPR Plasmids CRISPR Guide Addgene Blog Posts Addgene is proud...multiple sgRNAs may be expressed from a single plasmid. Add “CACC” before the 20-mer guide sequence and... protocol involves the delivery of CRISPR/Cas9 plasmids by electroporation 4 . This protocol is described...However, transient transfection of CRISPR/Cas9 plasmids may be successfully adapted to numerous cell ...

...function in cancer. p53 Pathway Plasmids Click on a name to find available plasmids for the gene, or browse the...suggestions for other plasmids that should be added to this list? Fill out our Suggest a Plasmid form or e-mail...Addgene's collection of plasmids for the p53 pathway. p53 is a transcription factor and tumor suppressor...mTOR pathway Ras pathway Background p53 Pathway Plasmids p53 Gene List References Background p53 is a transcription... - Gene List Click on a name to find available plasmids for the gene. For components with multiple isoforms...

...Generally, you will need two plasmids, as depicted in the figure below: A plasmid expressing the tRNA and ... included. Browse Synthetase Plasmids The table below highlights plasmids that contain aminoacyl tRNA ...Many of the plasmids also contain one or more copies of the cognate tRNA gene. ID Plasmid Synthetase Origin...phosphoserine Ryan Mehl Browse Target Plasmids The table below highlights plasmids that contain genes with modified...Addgene's guide to plasmids and strains useful for expanding the genetic code in bacteria and mammalian... Plasmid Collections Genetic Code Expansion Genetic Code Expansion You may also like... GCE4All...tRNA synthetases are introduced into the cell on plasmids and the new amino acid is introduced in the media...

...collaboration with the labs who have deposited CRISPR plasmids , we've created a series of links and guides to...on Addgene's blog Protocols Lab(s) Description Plasmids in protocol Download protocol Church gRNA design...gRNA design and cloning for Cas9 orthologs Cas9 plasmids PDF 104.1 KB Chen and Wente Zebrafish: gRNA cloning...(Cre recombinase) PDF 102.5 KB Jaenisch CRISPR plasmid summary for transcriptional activation dCas9 activators... proteins pFETCh_Donor ; additional HDR & gRNA plasmids are available for CREB1, ATF1, GABPA, & RAD21 ... Sontheimer gRNA design and cloning All-in-one plasmid containing expression cassette for NmCas9 and BsmBI...design; Deep sequencing Cas9: pMLM3613 dsDNA donor plasmid backbones pSL1180-HR-PUbECFP & pSL1180polyUBdsRED...

...provide important details including the protein and plasmid sequence, the antigen sequence, and cross-species...develop and refine these application lists. The plasmids we use to produce antibodies are available as ...as part of Addgene’s larger Antibody Plasmid Collection , which also includes other types of affinity reagents...? Check out our current collection of Antibody Plasmids , fill out our Suggest an Antibody form, or email...

... Special Collections EXPRESs Plasmids Human Platelet EXPRESs Plasmids for Human Platelet Receptors You...may also like... Gavin Wright Lab plasmids This is a collection plasmids for expressing secreted recombinant... 2015 May; 14(5):1265-74. PubMed 25713122 Plasmid ID Plasmid Name 51623 ACVR1-bio-His 51624 PECAM1-bio-His...

... low levels of co-purified plasmid from transfection. This residual plasmid is extracted and amplified...embryonic kidney cells are transfected with the plasmid(s) encoding the heavy and light chains. Antibodies...control and the protein size is confirmed. Residual Plasmid Sanger Sequencing Purified recombinant antibody...the Sanger method, and aligned with the parental plasmid sequence to confirm the antibody’s identity. For...

...Additionally, many plasmids containing validated gRNAs are now available through Addgene. These plasmids contain ...particular model organism. There may not be a perfect plasmid for your specific application, and in such cases...different activators exist, including the multi-plasmid SAM system. Select Expression System To use CRISPR...to generate stable cell lines (for details, see Plasmids 101: Mammalian Vectors ) Transient or stable expression...and enrich positive cells Packaging and envelope plasmids provide the necessary components to make lentiviral...Read more about how to design your gRNA . Browse Plasmids: gRNAs Synthesize and clone desired gRNAs Once...oligos are synthesized, annealed, and inserted into plasmids containing the gRNA scaffold using standard restriction-ligation...

...human cancers. Ras Pathway Plasmids Click on a name to find available plasmids for the gene, or browse the...suggestions for other plasmids that should be added to this list? Fill out our Suggest a Plasmid form or e-mail...Addgene's collection of plasmids for the Ras pathway. Ras is a small GTPase and constitutively active...mTOR Pathway p53 Pathway Background Ras Pathway Plasmids Ras Gene List Resources Background Ras is a small... - Gene List Click on a name to find available plasmids for the gene. For components with multiple subunits...

...preparations were produced with the pUCmini-iCAP-PHP.eB plasmid (Addgene #103005) . Note on PHP.eB tropism and ...preparations were produced with the pUCmini-iCAP-PHP.S plasmid (Addgene #103006) . Browse Available PHP.S AAV ...preparations were produced with the pUCmini-iCAP-PHP.V1 plasmid (Addgene #127847) . Browse Available PHP.V1 AAV...were produced with the pUCmini-iCAP-AAV.MaCPNS1 plasmid (Addgene #185136) . Browse Available MaCPNS1 AAV...were produced with the pUCmini-iCAP-AAV.MaCPNS2 plasmid (Addgene #185137) . Browse Available MaCPNS2 AAV...were produced with the pUCmini-iCAP-AAV.CAP-B10 plasmid (Addgene #175004) . Browse Available CAP-B10 AAV...were produced with the pUCmini-iCAP-AAV.CAP-B22 plasmid (Addgene #175005) . Browse Available CAP-B22 AAV...

... Biosensors Luciferase Plasmids Subcellular Localization Optogenetics Plasmids Viral Service: AAV Biosensors...suggestions for other plasmids that should be added to this list? Fill out our Suggest a Plasmid form or e-mail...Collection of plasmids encoding fluorescent biosensors to measure biomolecules or genes via FRET or other... Plasmid Collections Fluorescent Proteins Fluorescent Biosensors Fluorescent Proteins: Biosensors...our catalog . Use the article links to find the plasmids associated with the article. We also offer ready-to-use...ready-to-use AAV preparations of select plasmids; these are noted with the viral service icon . Metal Ions... localized dTomato fluorophore Ting Lab GCaMP6 Plasmids 2014 Jonathan Ting Calcium Intersectional viral...

...This page lists plasmid vectors designed to express nucleases in different cell types. Zinc finger arrays... Plasmid Collections Zinc Finger Consortium Nuclease Expression...Consortium: Nuclease Expression Vectors This page lists plasmid vectors designed to express nucleases in different...

...Guide to TALEN technology and using TALEN plasmids...Technologies You may also like... TALEN Kits CRISPR Plasmids Zinc Finger Consortium Originally published in... 2011, the groups describe a set of customized plasmids that can be used with the recently developed Golden...ordered fashion in a single reaction.” Using these plasmids, which are available as a kit from Addgene , custom... TAL effectors could become a key tool in your plasmid toolbox. Addgene has rapidly become an important...

...reporter gene in cultured U2OS cells. EGFP TALEN Plasmid Plasmid Name Target Half-Site eGFP-TALEN-01-Left TAL2000...Pre-constructed pairs of TALEN plasmids targeting 48 sites in the EGFP reporter gene. TALengineering... Nat Biotechnol. 2012 ). The table below lists plasmids encoding these 48 pairs of TALENs that were shown...

...publications associated with Addgene materials. Plasmids ID Plasmid Description PI Viral Preps Addgene distributes...research. Browse the BRAIN Initiative collection of plasmids, viral preps, and antibodies here. Collections...dissemination to the research community. Browse plasmids , viral preps , and antibodies created with support...distributes ready-to-use viral preps made from plasmids in our collection! AAV are distributed as high-quality...pAAV-CAG-FLEX-Archon1-KGC-EGFP-ER2 AAV production plasmid encoding for Archon1 fluorescent voltage reporter...IRES EGFP Chemogenetics Cre-dependent expression plasmid Scott Sternson 119741-AAV9 AAV SYN flex PSAM4 GlyR...IRES EGFP Chemogenetics Cre-dependent expression plasmid Scott Sternson 119742-AAV5 AAV SYN PSAM4 GlyR IRES...

...was developed by Shannon Boye’s lab. Plasmid Information The plasmid below is available in each of the above...We provide high quality AAV preps from select plasmids in the repository. Browse the University of Florida... preparations were produced with the pTR-UF11 plasmid ( Addgene #157970 ). Viral preparations are formulated...above serotypes and buffers. Please click on the plasmid name for ordering information. ID Name Promoter...

... BRAIN Armamentarium Project below. Plasmids ID Plasmid Description PI Viral Preps ID Viral Prep Description...underlie behavior and network function. Browse plasmids or viral preps created with support from the BRAIN...

...Adenoviral Guide Viral Vector Plasmids and Resources Addgene Blog: Your Lentiviral Plasmid FAQs Biosafety Resource...reporter plasmids to test whether or not you’ve efficiently generated infectious virus, plasmids expressing...

...suggestions for other plasmids that should be added to this list? Fill out our Suggest a Plasmid form or e-mail...Plasmids encoding fluorescent proteins tagged with genes or peptides with known subcellular localization... Plasmid Collections Fluorescent Proteins Organelle Markers...Empty Backbones Allen Institute for Cell Science Plasmid Collection FRET Biosensors Optogenetics Fluorescent...general cellular organelles and structures. ID Plasmid Structure Targeting Gene/Signal Fluorescent Protein...below to find markers of cytoskeletal element. ID Plasmid Structure Targeting Gene/Signal Fluorescent Protein...below to find markers for cellular junctions. ID Plasmid Structure Targeting Gene/Signal Fluorescent Protein...