... on the plasmid. gRNA plasmids that do not co-express a Cas protein require a separate plasmid that does...gRNA Vectors CRISPR Plasmids: Empty gRNA Expression Vectors Browse CRISPR Plasmids By Function Genome ... these independent gRNA plasmids can be paired with a wide variety of plasmids and therefore are not limited.... Whether the plasmid contains Cas9 and if so, which function of Cas9. We have plasmids with Cas9 that...none, need plasmid 65775 N. meningitidis Joung VVT1 65779 Mammalian BsmBI none, need plasmid 65776 S. aureus...expression of Csy4 (eg Plasmid 53369 ). Joung pX333 Mammalian Single all-in-one plasmid for tandem expression...types of plasmids available for use in this Golden Gate and Gateway based assembly method: plasmids for assembly...

...suggestions for other plasmids that should be added to this list? Fill out our Suggest a Plasmid form or e-mail...CRISPR/Cas plasmids for labeling and visualization of genomic loci. CRISPR... CRISPR Visualize CRISPR Plasmids: Visualize...Visualize Genomic Loci Browse CRISPR Plasmids By Function Genome Editing Cut Base Edit Nick Prime Edit Transcriptional...target sites throughout the chromosome. Mammalian Plasmid Gene/Insert Promoter Selectable Marker PI Publication...

...Keith Joung Lab plasmids CRISPR plasmids Zinc Finger Consortium This page lists plasmid vectors into which...-Fast assembly or names of plasmids required for FLASH assembly. ID Plasmid Activation Domain RVD of C-terminal...activators made on the framework encoded in these plasmids robustly yields highly active artificial transcription...

...like... Keith Joung Lab plasmids Daniel Voytas Lab plasmids Scot Wolfe Lab plasmids The Zinc Finger Consortium... Plasmid Collections Zinc Finger Consortium Zinc Finger ...Technologies TALEN Engineering Reagents CRISPR Plasmids...

... CRISPR Boxem Lab CRISPR Plasmids Boxem Lab CRISPR Plasmids Available... 23979586 . Individual plasmids can be ordered via the links below: ID Plasmid 47941 pMB60 : Can be used...gRNA plasmids from Dr. Mike Boxem's lab. CRISPR...oligonucleotide linker into BsaI digested vector. These plasmids are described in: CRISPR/Cas9-Targeted Mutagenesis...

... Special Collections EXPRESs Plasmids Human Platelet EXPRESs Plasmids for Human Erythrocyte Receptors...may also like... Gavin Wright Lab plasmids This is a collection plasmids for expressing secreted recombinant...2011 Nov 9;480(7378):534-7. PubMed 22080952 Plasmid ID Plasmid Name 73089 bio-CD4d3+4-Kell blood group 73090...

...Species) Organism/Expression System Plasmids Mammalian Golenbock Lab Plasmids - Includes tagging with CFP, YFP...Lentiviral Plasmids - Fluorescently tag your gene for lentiviral expression Verkhusha Lab Plasmids - Enhanced...Mammalian Plasmids - Includes tagging with mCherry, mCitrine, mCerulean Davidson Lab Plasmids - Includes...suggestions for other plasmids that should be added to this list? Fill out our Suggest a Plasmid form or e-mail...Addgene has assembled a collection of empty plasmid backbones with different fluorescent tags for you...colors. Addgene has assembled a collection of empty plasmid backbones with different fluorescent tags for you...Emission (nm) Brightness pKa Maturation Structure Plasmids Sirius 355 424 4 3.0 Prone to dimerization Sirius-N1...

...download from each plasmid page. Use the search bar or sort buttons to find plasmids based on: Organism...collaborations between institutions. With these plasmids, faculty can begin a sustainable MDH-related CURE...and metabolic pathways and protein interactions. Plasmids A diverse set of wild-type MDH genes from several...the C-terminus of MDH and, if indicated on the plasmid map, a TEV cleavage site is present between the..."active site loop", or "subunit interface") ID Plasmid Description Gene/Insert Mutations Other Resources...opens in a new window) Acknowledgments The MCC and plasmid construction was funded by NSF IUSE 1726932 and...

...Looking for Antibody Plasmids? Browse our collection of affinity reagent plasmids - including antibodies...affinity reagents are expressed and purified from plasmids in our repository. Antibodies...collection of affinity reagents that are expressed from plasmids. These antibodies undergo application-specific...antibodies, nanobodies, scFvs, and more! Find Plasmids...

...delivery of a plasmid. Below, browse our selection of empty vectors for cloning in shRNAs. Plasmids for constitutive...Plasmids for mammalian RNA interference (RNAi) experiments at Addgene; browse by constitutive lentiviral... Plasmid Collections Mammalian RNAi Mammalian shRNA Tools... libraries Lentiviral packaging and expression plasmids Background RNA interference (RNAi) is an RNA-mediated...inducible (Tet) expression are available. To find plasmids containing RNAi components, like Argonaute, please...Lentiviral vectors for constitutive shRNA expression ID Plasmid Description PI 10878 pLKO.1 ‐ TRC cloning vector... for conditional (Cre-lox) shRNA expression ID Plasmid Description PI 11578 pSico Cre addition causes ...

...Collections INTRSECT Plasmid Collection INTRSECT Plasmid Collection INTRSECT Implementation Plasmids References ...novel INTSRECT plasmids for groups interested in producing their own INTRSECT plasmids. Online spreadsheet...Find intersectional expression plasmids that combine multiplexed recombinases, including Cre, Flp, and...Flp-recombinase-expressing transgenic mouse and rat lines. Plasmids In addition to EYFP and ChR2-EYFP, a large number...rabies targeting genes. Recombinases Addgene ID Plasmid Logic Sites and Mutations 55636 pAAV-EF1a-Cre None...pAAV-CaMKIIa-Flpo None Single recombinase-dependent Addgene ID Plasmid Logic Sites and Mutations 55641 pAAV-Ef1a-fDIO ...recombinase-dependent: Fluorophores Addgene ID Plasmid Logic Sites and Mutations 55650 pAAV-hSyn Con/Fon...

...multiplex gRNA plasmid Bullock 73915 pCFD6 tissue-specific multiplex gRNA plasmid Bullock Cas9 plasmids ID Name...consisting of transgenic cas9 fly lines and gRNA plasmids. Project tools can be used for both generating... refer to the CRISPR Fly Design homepage. gRNA plasmids ID Name Description PI 49408 pCFD1-dU6:1gRNA expression...

...Rudolf Jaenisch labs Plasmids MECP2 Plasmids The table below highlights plasmids that express or target...information on generating iPSCs using plasmids, please see Addgene's Plasmids for Stem Cell Research page. CRISPR...Broswe plasmids and other research tools for use in Rett Syndrome research. Plasmid... Plasmid Collections Rett ...Rett Syndrome Rett Syndrome Plasmid Collection and Resource Center MECP2 Animal Models Human Cell Lines CRISPR... CRISPR Tools Plasmids Resources The (Link opens in a new window) Rett Syndrome Research Trust (RSRT) ...individual laboratories to assemble a Rett Syndrome plasmid resource for the scientific research community....

...From the Bikard Lab, CRISPR plasmid collection for modulating expression levels of two targets independently...Repression Collection Bikard Lab CRISPR Repression Plasmids You may also like... CRISPR Guide CRISPR Protocols...expression levels. This collection comprises 20 plasmids, each carrying two CRISPR guides. The first one...18 or 20 matching nucleotides. Together, these plasmids allow to explore the whole range of expression...amount and stoichiometry of enzymes. The pLC97 plasmid allows for quick integration of the aTc-inducible...

...suggestions for other plasmids that should be added to this list? Fill out our Suggest a Plasmid form or e-mail...Addgene's collection of plasmids suitable for FRET experiments to measure protein interactions or conformation... Plasmid Collections Fluorescent Proteins FRET Fluorescent...these types of studies Addgene has a collection of plasmids suitable for creating individual fluorescently...Encoding Fluorescent Proteins for FRET The following plasmids can be used to create a fluorescent fusion protein...of interest and the listed fluorescent protein. Plasmid Color Expression Description pPROEX Aqua Cyan Bacterial... to Top FRET Reference Standards The following plasmids have well-characterized FRET efficiency values...

...the transfer plasmid, a plasmid encoding Rep and serotype-specific Cap genes, and a plasmid encoding adenoviral... coli . In addition, plasmids are typically prepared using endotoxin-free plasmid purification kits. Resources...contamination is minimized by using an endotoxin-free plasmid purification protocol. To minimize the immunogenic...off-line and discarded. Confirmation of Transfer Plasmid Addgene uses a rigorous barcode matching system...microbial contamination. Purity When possible, all plasmids used for viral production are propagated in the...experiment using our virus protocols . Browse our plasmid collection of popular lentiviral vectors ....

...proliferation. mTOR Pathway Plasmids Click on a name to find available plasmids for the gene, or browse the...suggestions for other plasmids that should be added to this list? Fill out our Suggest a Plasmid form or e-mail...Addgene's plasmids for the mTOR pathway. enhanced signaling of mTOR, a key metabolic regulator, correlates...with a New Screening Kit Background mTOR Pathway Plasmids mTOR Gene List Resources The m echanistic or m... - Gene List Click on a name to find available plasmids for the gene. For components with multiple isoforms...

...genetic variants and disease associations. Plasmids This plasmid collection contains codon-optimized ORF .... Browse the SLC plasmid collection. Depositor... Depositor Collections Resolute RESOLUTE Plasmid Collection You may... range of Gateway-compatible pDEST vectors. ID Plasmid Gene/Insert Industry PI Pooled Libraries CRISPR...

...AAV Vectors for Serotype Testing pAAV-CAG-GFP (Plasmid #37825) Description : Ready-to-use AAV in various... for direct injection. These AAV are made from plasmid 37825 (deposited by Edward Boyden ) and direct ...AAVrg.T 20 µL $ 140 Add to Cart pAAV-hSyn-EGFP (Plasmid #50465) Description : Ready-to-use AAV in various... for direct injection. These AAV are made from plasmid 50465 (deposited by Bryan Roth ) and direct EGFP...a sample of the purified DNA from the original plasmid that was used to make the virus. For more details...

...are made from plasmids that are currently in our repository. You can obtain the plasmid from us or receive... list of gRNA plasmids targeting specific genes. For more information on CRISPR plasmids and function,...processes. For more information, see our Guide to Plasmid Pooled Libraries . ID Name Description Genes/Insert...function, see our CRISPR plasmids resource page . Browse our list of available Cas9 viruses by function: Edit... Browse our list of available viruses based on plasmids in our repository that express fluorescent proteins... Browse our list of available viruses based on plasmids in our repository that are related to COVID-19...