...collection of synthetic biology plasmids for use in C. elegans. Worm Plasmids Search the table by keyword ...to view all plasmids available from the article. Click on the PI link to view all plasmids available from...suggestions for other plasmids that should be added to this list? Fill out our Suggest a Plasmid form or e-mail...collection of synthetic biology plasmids for use in C. elegans. Plasmid... Plasmid Collections Synthetic Biology Worm... from this depositor's lab. Plasmid Description Gene/Insert Vector Type PI Publication Do you have suggestions...

...collection of synthetic biology plasmids for use in yeast. Yeast Plasmids Search the table by keyword or...to view all plasmids available from the article. Click on the PI link to view all plasmids available from...suggestions for other plasmids that should be added to this list? Fill out our Suggest a Plasmid form or e-mail...Addgene's collection of synthetic biology plasmids for use in yeast. Plasmid... Plasmid Collections Synthetic Biology Yeast... from this depositor's lab. Plasmid Description Gene/Insert Vector Type PI Publication Do you have suggestions...

...v2 2 vector system: lentiCas9-Blast and lentiGuide-Puro packaging plasmids: pVSVg , psPAX2 positive control...collaboration with the labs who have deposited CRISPR plasmids , we've created a series of links and guides to...Mammalian Cell Lines CasPEDIA : An encyclopedia of Class 2 CRISPR systems with wiki entries describing enzyme...on Addgene's blog Protocols Lab(s) Description Plasmids in protocol Download protocol Church gRNA design...gRNA design and cloning for Cas9 orthologs Cas9 plasmids PDF 104.1 KB Chen and Wente Zebrafish: gRNA cloning...(Cre recombinase) PDF 102.5 KB Jaenisch CRISPR plasmid summary for transcriptional activation dCas9 activators... proteins pFETCh_Donor ; additional HDR & gRNA plasmids are available for CREB1, ATF1, GABPA, & RAD21 ...

...to view all plasmids available from the article. Click on the PI link to view all plasmids available from...suggestions for other plasmids that should be added to this list? Fill out our Suggest a Plasmid form or e-mail...Synthetic Biology plasmids for Sensing and Signaling. Plasmid... Plasmid Collections Synthetic Biology Sensing and...Browse Addgene's collection of synthetic biology plasmids related to cell-to-cell communications and environmental...environmental sensing. Sensing and Signaling Plasmids Search the table by keyword or sort by the table ... from this depositor's lab. Plasmid Gene/Insert Vector Type Promoter Tags PI Publication Do you have suggestions...

...to view all plasmids available from the article. Click on the PI link to view all plasmids available from...suggestions for other plasmids that should be added to this list? Fill out our Suggest a Plasmid form or e-mail...Synthetic Biology plasmids for metabolic enzymes and pathways. Plasmid... Plasmid Collections Synthetic Biology ...Browse Addgene's collection of synthetic biology plasmids related to metabolic pathways and components. ... Pathways Efflux Pumps Flux Control Metabolism Plasmids Search the table by keyword or sort by the table... from this depositor's lab. Plasmid Gene/Insert Vector Type Promoter PI Publication Back to Top Do you...

...delivery of a plasmid. Below, browse our selection of empty vectors for cloning in shRNAs. Plasmids for constitutive...Plasmids for mammalian RNA interference (RNAi) experiments at Addgene; browse by constitutive lentiviral... Plasmid Collections Mammalian RNAi Mammalian shRNA Tools... libraries Lentiviral packaging and expression plasmids Background RNA interference (RNAi) is an RNA-mediated...inducible (Tet) expression are available. To find plasmids containing RNAi components, like Argonaute, please...Lentiviral vectors for constitutive shRNA expression ID Plasmid Description PI 10878 pLKO.1 ‐ TRC cloning vector... for conditional (Cre-lox) shRNA expression ID Plasmid Description PI 11578 pSico Cre addition causes ...

...to view all plasmids available from the article. Click on the PI link to view all plasmids available from...suggestions for other plasmids that should be added to this list? Fill out our Suggest a Plasmid form or e-mail...collection of synthetic biology plasmids related to Networks and Gene Regulation Plasmid... Plasmid Collections Synthetic ...Browse Addgene's collection of synthetic biology plasmids related to Networks and Regulation. The collection...activators logic gates Networks and Gene Regulation Plasmids Search the table by keyword or sort by the table... from this depositor's lab. Plasmid Gene/Insert Vector Type Promoter PI Publication Back to Top Do you...

...like... Keith Joung Lab plasmids Daniel Voytas Lab plasmids Scot Wolfe Lab plasmids Detailed Information ... room temperature. Individual plasmids can be ordered from each plasmid page and will be shipped as bacterial...Depositing Labs: Keith Joung This kit consists of plasmids and strains used to engineer ZFNs via the Oligomerized.... This kit consists of 5 individually packaged plasmids and 3 individually packaged strains, which are...Nature Protocols paper . How to Cite this Kit These plasmids were created by your colleagues. Please acknowledge.... For your Materials and Methods section: "The plasmid kit used in this publication was a gift from Keith...

...download from each plasmid page. Use the search bar or sort buttons to find plasmids based on: Organism...Streptomyces ) Subcellular compartment (chloroplastic, cytoplasmic, glyoxysomal, mitochondrial, peroxisomal...collaborations between institutions. With these plasmids, faculty can begin a sustainable MDH-related CURE...and metabolic pathways and protein interactions. Plasmids A diverse set of wild-type MDH genes from several...the C-terminus of MDH and, if indicated on the plasmid map, a TEV cleavage site is present between the..."active site loop", or "subunit interface") ID Plasmid Description Gene/Insert Mutations Additional Resources...opens in a new window) Acknowledgments The MCC and plasmid construction was funded by NSF IUSE 1726932 and...

...suggestions for other plasmids that should be added to this list? Fill out our Suggest a Plasmid form or e-mail...Addgene's collection of plasmids suitable for FRET experiments to measure protein interactions or conformation... Plasmid Collections Fluorescent Proteins FRET Fluorescent...these types of studies Addgene has a collection of plasmids suitable for creating individual fluorescently...Encoding Fluorescent Proteins for FRET The following plasmids can be used to create a fluorescent fusion protein...of interest and the listed fluorescent protein. Plasmid Color Expression Description pPROEX Aqua Cyan Bacterial... to Top FRET Reference Standards The following plasmids have well-characterized FRET efficiency values...

...cell-specific manipulation. Plasmids Fluorescent Protein Purpose Principal Investigator Plasmids GFP GFP-dependent...Connie Cepko See Plasmids Dronpa Optical control of protein activity Michael Lin See Plasmids Do you have ...suggestions for other plasmids that should be added to this list? Fill out our Suggest a Plasmid form or e-mail...Plasmids using a fluorescent protein as a scaffold to allow scientists to selectively modulate gene expression... Plasmid Collections Fluorescent Proteins Activity Regulation Fluorescent Proteins: Activity...

...Additionally, many plasmids containing validated gRNAs are now available through Addgene. These plasmids contain ...particular model organism. There may not be a perfect plasmid for your specific application, and in such cases...different activators exist, including the multi-plasmid SAM system. Select Expression System To use CRISPR...to generate stable cell lines (for details, see Plasmids 101: Mammalian Vectors ) Transient or stable expression...and enrich positive cells Packaging and envelope plasmids provide the necessary components to make lentiviral...Read more about how to design your gRNA . Browse Plasmids: gRNAs Synthesize and clone desired gRNAs Once...oligos are synthesized, annealed, and inserted into plasmids containing the gRNA scaffold using standard restriction-ligation...

...From the Bikard Lab, CRISPR plasmid collection for modulating expression levels of two targets independently...Repression Collection Bikard Lab CRISPR Repression Plasmids You may also like... CRISPR Guide CRISPR Protocols...expression levels. This collection comprises 20 plasmids, each carrying two CRISPR guides. The first one...18, or 20 matching nucleotides. Together, these plasmids allow to explore the whole range of expression...amount and stoichiometry of enzymes. The pLC97 plasmid allows for quick integration of the aTc-inducible... sfgfp in this case, can be controlled using a plasmid‐borne CRISPR array coding for guide RNAs (diamonds...high‐throughput microscopy for a collection of 20 CRISPR plasmids. Each point represents a single cell, and each...

...proliferation. mTOR Pathway Plasmids Click on a name to find available plasmids for the gene, or browse the...suggestions for other plasmids that should be added to this list? Fill out our Suggest a Plasmid form or e-mail...Addgene's plasmids for the mTOR pathway. enhanced signaling of mTOR, a key metabolic regulator, correlates...with a New Screening Kit Background mTOR Pathway Plasmids mTOR Gene List Resources The m echanistic or m... - Gene List Click on a name to find available plasmids for the gene. For components with multiple isoforms...bisphosphate 3-kinase catalytic and regulatory subunits - Class I PKC PKC alpha PKC beta PKC delta PKC epsilon PKC...

... SH, Thompson T, Karsenty G, Bradley A, Donehower LA. Nature. 2002 Jan 3;415(6867):45-53. PubMed PMID:...function in cancer. p53 Pathway Plasmids Click on a name to find available plasmids for the gene, or browse the...suggestions for other plasmids that should be added to this list? Fill out our Suggest a Plasmid form or e-mail...Addgene's collection of plasmids for the p53 pathway. p53 is a transcription factor and tumor suppressor...mTOR pathway Ras pathway Background p53 Pathway Plasmids p53 Gene List References Background p53 is a transcription... - Gene List Click on a name to find available plasmids for the gene. For components with multiple isoforms... Serpin peptidase inhibitor, clade E (nexin, plasminogen activator inhibitor type 1), member 1 PERP TP53...

...preparations were produced with the pUCmini-iCAP-PHP.eB plasmid (Addgene #103005) . Note on PHP.eB tropism and ...preparations were produced with the pUCmini-iCAP-PHP.S plasmid (Addgene #103006) . Browse Available PHP.S AAV ...preparations were produced with the pUCmini-iCAP-PHP.V1 plasmid (Addgene #127847) . Browse Available PHP.V1 AAV...were produced with the pUCmini-iCAP-AAV.MaCPNS1 plasmid (Addgene #185136) . Browse Available MaCPNS1 AAV...were produced with the pUCmini-iCAP-AAV.MaCPNS2 plasmid (Addgene #185137) . Browse Available MaCPNS2 AAV...were produced with the pUCmini-iCAP-AAV.CAP-B10 plasmid (Addgene #175004) . Browse Available CAP-B10 AAV...were produced with the pUCmini-iCAP-AAV.CAP-B22 plasmid (Addgene #175005) . Browse Available CAP-B22 AAV...

...Consortium Plasmids Browse All SGC Plasmids Structural Genomics Consortium Plasmids All SGC Plasmids Plasmid...Browse plasmids from the Structural Genomics Consortium (SGC) for 3D protein structure studies Plasmid...

... Zhang Lab Plasmids The Human TFome Library Stem Cells Protocols & References MORF Plasmids MORF Pooled...out PCR for 10X scRNA-seq (PDF, 59.2 KB) Plasmids ID Plasmid Gene/Insert Pooled Library The MORF pooled...suggestions for other plasmids that should be added to this list? Fill out our Suggest a Plasmid form or e-mail.... Protocols & References A transcription factor atlas of directed differentiation. Joung J, Ma S, Tay ...

... 493(7432):429-32. PMID: 23242138 Chen B, Gilbert LA, Cimini BA, Schnitzbauer J, Zhang W, Li GW, Park ...Barretto R, Habib N, Hsu PD, Wu X, Jiang W, Marraffini LA, Zhang F. 2013. Multiplex genome engineering using...Res Commun . 439(1):132-6. PMID: 23942116 Gilbert LA, Horlbeck MA, Adamson B, Villalta JE, Chen Y, Whitehead...Activation. Cell . 159(3):647-6. PMID: 25307932 Gilbert LA, Larson MH, Morsut L, Liu Z, Brar GA, Torres SE, ...with Type V following in 2015. Class 1 (Multi-subunit effector complex) Class 2 (Single multi-domain effector...posts , or find CRISPR plasmids for your research. Addgene has CRISPR plasmids for the following models...number/types of Cas proteins. Makarova et al. ’s classification defines 5 types and 16 subtypes based on shared...

...Browse Antibody Plasmids The following plasmids can be used to create or express plasmid-based antibodies...epitope tags, as well as antibody plasmids to create or express plasmid-based antibodies. The majority of...Integrin Antibodies Epitope Tag Antibodies Antibody Plasmids Welcome to the Institute for Protein Innovation...antibodies. ID Plasmid Description...