We narrowed to 160 results for: plasmid
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TypeCollection... on the plasmid. gRNA plasmids that do not co-express a Cas protein require a separate plasmid that does...gRNA Vectors CRISPR Plasmids: Empty gRNA Expression Vectors Browse CRISPR Plasmids By Function Genome ... these independent gRNA plasmids can be paired with a wide variety of plasmids and therefore are not limited.... Whether the plasmid contains Cas9 and if so, which function of Cas9. We have plasmids with Cas9 that...none, need plasmid 65775 N. meningitidis Joung VVT1 65779 Mammalian BsmBI none, need plasmid 65776 S. aureus...expression of Csy4 (eg Plasmid 53369 ). Joung pX333 Mammalian Single all-in-one plasmid for tandem expression...types of plasmids available for use in this Golden Gate and Gateway based assembly method: plasmids for assembly...
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Fluorescent Protein Guide: Empty Backbones
TypeCollection...Species) Organism/Expression System Plasmids Mammalian Golenbock Lab Plasmids - Includes tagging with CFP, YFP...Lentiviral Plasmids - Fluorescently tag your gene for lentiviral expression Verkhusha Lab Plasmids - Enhanced...Mammalian Plasmids - Includes tagging with mCherry, mCitrine, mCerulean Davidson Lab Plasmids - Includes...suggestions for other plasmids that should be added to this list? Fill out our Suggest a Plasmid form or e-mail...Addgene has assembled a collection of empty plasmid backbones with different fluorescent tags for you...colors. Addgene has assembled a collection of empty plasmid backbones with different fluorescent tags for you...Emission (nm) Brightness pKa Maturation Structure Plasmids Sirius 355 424 4 3.0 Prone to dimerization Sirius-N1... -
Cancer Research Plasmids and Resources
TypeCollection...component to find plasmids with your gene of interest. Browse Cancer Research Plasmids by Pathway mTOR ...Addgene's curated collection of plasmids for cancer research, organized by signaling pathway. Special...Special Collections Cancer Research Cancer Research Plasmids and Resources Addgene has a large variety of resources...Each collection page listed below organizes the plasmids and resources by signaling pathway, focusing on...Libraries for Cancer Research Broad Target Accelerator Plasmids: Collection of vectors from the Boehm , Hahn ,... -
Feng Zhang Multiplexed Overexpression of Regulatory Factors (MORF) Collection
TypeCollection... Zhang Lab Plasmids The Human TFome Library Stem Cells Protocols & References MORF Plasmids MORF Pooled...out PCR for 10X scRNA-seq (PDF, 59.2 KB) Plasmids ID Plasmid Gene/Insert Pooled Library The MORF pooled...suggestions for other plasmids that should be added to this list? Fill out our Suggest a Plasmid form or e-mail... -
CRISPR Plasmids for Genomic Visualization
TypeCollection...suggestions for other plasmids that should be added to this list? Fill out our Suggest a Plasmid form or e-mail...CRISPR/Cas plasmids for labeling and visualization of genomic loci. CRISPR... CRISPR Visualize CRISPR Plasmids: Visualize...Visualize Genomic Loci Browse CRISPR Plasmids By Function Genome Editing Cut Base Edit Nick Prime Edit Transcriptional... sites throughout the chromosome. Mammalian ID Plasmid Gene/Insert Promoter Selectable Marker PI Publication... -
Zinc Finger Consortium Reagents
TypeCollection...like... Keith Joung Lab plasmids Daniel Voytas Lab plasmids Scot Wolfe Lab plasmids The Zinc Finger Consortium... Plasmid Collections Zinc Finger Consortium Zinc Finger ...Technologies TALEN Engineering Reagents CRISPR Plasmids... -
Malate Dehydrogenase CUREs Community Collection
TypeCollection...download from each plasmid page. Use the search bar or sort buttons to find plasmids based on: Organism...collaborations between institutions. With these plasmids, faculty can begin a sustainable MDH-related CURE...and metabolic pathways and protein interactions. Plasmids A diverse set of wild-type MDH genes from several...the C-terminus of MDH and, if indicated on the plasmid map, a TEV cleavage site is present between the..."active site loop", or "subunit interface") ID Plasmid Description Gene/Insert Mutations Additional Resources...opens in a new window) Acknowledgments The MCC and plasmid construction was funded by NSF IUSE 1726932 and... -
Antibodies
TypeCollection...Looking for Antibody Plasmids? Browse our collection of affinity reagent plasmids - including antibodies...affinity reagents are expressed and purified from plasmids in our repository. Antibodies...collection of affinity reagents that are expressed from plasmids. These antibodies undergo application-specific...antibodies, nanobodies, scFvs, and more! Find Plasmids... -
Deisseroth INTRSECT Collection
TypeCollection...Collections INTRSECT Plasmid Collection INTRSECT Plasmid Collection INTRSECT Implementation Plasmids References ...novel INTSRECT plasmids for groups interested in producing their own INTRSECT plasmids. Online spreadsheet...Find intersectional expression plasmids that combine multiplexed recombinases, including Cre, Flp, and...that have used these constructs experimentally. Plasmids In addition to EYFP and ChR2-EYFP, a large number...rabies targeting genes. Recombinases Addgene ID Plasmid Logic Sites and Mutations In-Stock Viral Items ...None No Single recombinase-dependent Addgene ID Plasmid Logic Sites and Mutations In-Stock Viral Items ...recombinase-dependent: Fluorophores Addgene ID Plasmid Logic Sites and Mutations In-Stock Viral Items ... -
Resolute Plasmid Collection
TypeCollection...genetic variants and disease associations. Plasmids This plasmid collection contains codon-optimized ORF .... Browse the SLC plasmid collection. Depositor... Depositor Collections Resolute RESOLUTE Plasmid Collection You may...Brain Initiative Collection NeuroMab Collection Plasmids Pooled Libraries Feedback Solute carrier proteins... range of Gateway-compatible pDEST vectors. ID Plasmid Gene/Insert Industry PI Pooled Libraries CRISPR... -
Rett Syndrome
TypeCollection...Rudolf Jaenisch labs Plasmids MECP2 Plasmids The table below highlights plasmids that express or target...information on generating iPSCs using plasmids, please see Addgene's Plasmids for Stem Cell Research page. CRISPR...Broswe plasmids and other research tools for use in Rett Syndrome research. Plasmid... Plasmid Collections Rett ...Rett Syndrome Rett Syndrome Plasmid Collection and Resource Center MECP2 Animal Models Human Cell Lines CRISPR... CRISPR Tools Plasmids Resources The (Link opens in a new window) Rett Syndrome Research Trust (RSRT) ...individual laboratories to assemble a Rett Syndrome plasmid resource for the scientific research community.... -
Fluorescent Protein Guide: FRET
TypeCollection...suggestions for other plasmids that should be added to this list? Fill out our Suggest a Plasmid form or e-mail...Addgene's collection of plasmids suitable for FRET experiments to measure protein interactions or conformation... Plasmid Collections Fluorescent Proteins FRET Fluorescent...these types of studies Addgene has a collection of plasmids suitable for creating individual fluorescently...Encoding Fluorescent Proteins for FRET The following plasmids can be used to create a fluorescent fusion protein...of interest and the listed fluorescent protein. Plasmid Color Expression Description pPROEX Aqua Cyan Bacterial... to Top FRET Reference Standards The following plasmids have well-characterized FRET efficiency values... -
Serotype Testing AAV
TypeCollection...AAV Vectors for Serotype Testing pAAV-CAG-GFP (Plasmid #37825) Description : Ready-to-use AAV in various... for direct injection. These AAV are made from plasmid 37825 (deposited by Edward Boyden ) and direct ...AAVrg.T 20 µL $ 140 Add to Cart pAAV-hSyn-EGFP (Plasmid #50465) Description : Ready-to-use AAV in various... for direct injection. These AAV are made from plasmid 50465 (deposited by Bryan Roth ) and direct EGFP...a sample of the purified DNA from the original plasmid that was used to make the virus. For more details...options. Select from a vast catalog of eligible AAV plasmids and we’ll make the viral vector prep you need.... -
Viral Production
TypeCollection...the transfer plasmid, a plasmid encoding Rep and serotype-specific Cap genes, and a plasmid encoding adenoviral... coli . In addition, plasmids are typically prepared using endotoxin-free plasmid purification kits. Resources...contamination is minimized by using an endotoxin-free plasmid purification protocol. To minimize the immunogenic...off-line and discarded. Confirmation of Transfer Plasmid Addgene uses a rigorous barcode matching system...microbial contamination. Purity When possible, all plasmids used for viral production are propagated in the...experiment using our virus protocols . Browse our plasmid collection of popular lentiviral vectors .... -
CRISPR References and Information
TypeCollection...Addgene CRISPR Resources CRISPR Plasmids Addgene's collection of CRISPR plasmids. CRISPR Guide Addgene's guide...guide to using CRISPR plasmids in your lab, from knockouts to pooled library screening. CRISPR eBook Addgene's...barcoding. CRISPR Kits Addgene's collection of CRISPR plasmid kits. CRISPR Software Sanger Indel Analysis ICE... Feng Zhang Lab . Protocols Lab(s) Description Plasmids in protocol Download protocol Addgene CRISPR pooled...gRNA design and cloning for Cas9 orthologs Cas9 plasmids PDF, 107 KB Chen and Wente Zebrafish: gRNA cloning... (Cre recombinase) PDF, 105 KB Jaenisch CRISPR plasmid summary for transcriptional activation dCas9 activators... proteins pFETCh_Donor ; additional HDR & gRNA plasmids are available for CREB1, ATF1, GABPA, and RAD21... -
Cre-lox system
TypeCollection... Plasmid Collections Cre-lox Plasmids Cre-lox Plasmids Additional...Cre-lox Plasmids Addgene's Blog: Cre-lox Addgene's Blog: FLEx Vectors Cre-containing Plasmids loxP Constructs...viral preparations of plasmids that are currently in our repository. ID Plasmid Description Promoter Expression...loxP plasmids, reporter genes indicate which cells have undergone Cre recombination. These plasmids can...suggestions for other plasmids that should be added to this list? Fill out our Suggest a Plasmid form or e-mail... Cre-lox plasmids for your experiments. Plasmid...more thorough introduction, check out Addgene’s Plasmids 101: Cre-lox blog post. Based on these Cre-lox... -
mTOR Pathway
TypeCollection...proliferation. mTOR Pathway Plasmids Click on a name to find available plasmids for the gene, or browse the...suggestions for other plasmids that should be added to this list? Fill out our Suggest a Plasmid form or e-mail...Addgene's plasmids for the mTOR pathway. enhanced signaling of mTOR, a key metabolic regulator, correlates...with a New Screening Kit Background mTOR Pathway Plasmids mTOR Gene List Resources The m echanistic or m... - Gene List Click on a name to find available plasmids for the gene. For components with multiple isoforms... -
Impact of Genomic Variation on Function (IGVF) Consortium Collection
TypeCollection...and phenotype and shares their plasmid tools through Addgene. ID Plasmid Description Vector Type PI Publication...Browse plasmid tools generated by IGVF Consortium investigators to examine how genomic variation affects...genomic variants on genome function and phenotypes. Plasmids The collection will grow as the IGVF community... -
Lentiviral Prep Service
TypeCollection...are made from plasmids that are currently in our repository. You can obtain the plasmid from us or receive... list of gRNA plasmids targeting specific genes. For more information on CRISPR plasmids and function,...processes. For more information, see our Guide to Plasmid Pooled Libraries . ID Name Description Genes/Insert...function, see our CRISPR plasmids resource page . Cas9 for Editing Genomic Sequences To perform genome edits,... Browse our list of available viruses based on plasmids in our repository that express fluorescent proteins... -
Genomic Deletions in Mammalian Cell Lines
TypeCollection...usage of the pSpCas9(BB) plasmid (pX330) (Addgene plasmid ID 42230). This plasmid allows for the simultaneous...utilized, such as pX458 (Addgene plasmid ID 48138) or pX459 (Addgene plasmid ID 48139), which include GFP ... with a PCR cloning kit) into a plasmid vector. Transform the plasmid into DH5α E. coli cells and plate...Mammalian Cell Lines Other Addgene Resources CRISPR Plasmids CRISPR Guide Addgene Blog Posts Addgene is proud...multiple sgRNAs may be expressed from a single plasmid. Add “CACC” before the 20-mer guide sequence and... protocol involves the delivery of CRISPR/Cas9 plasmids by electroporation 4 . This protocol is described...However, transient transfection of CRISPR/Cas9 plasmids may be successfully adapted to numerous cell ...