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We narrowed to 155 results for: plasmid

Showing: 101 - 120 of 155 results

  1. Cancer Research Plasmids and Resources

    Type
    Collection
    ...component to find plasmids with your gene of interest. Browse Cancer Research Plasmids by Pathway mTOR ...Addgene's curated collection of plasmids for cancer research, organized by signaling pathway. Special...Special Collections Cancer Research Cancer Research Plasmids and Resources Addgene has a large variety of resources...Each collection page listed below organizes the plasmids and resources by signaling pathway, focusing on...Libraries for Cancer Research Broad Target Accelerator Plasmids: Collection of vectors from the Boehm , Hahn ,...

  2. Feng Zhang Multiplexed Overexpression of Regulatory Factors (MORF) Collection

    Type
    Collection
    ... Zhang Lab Plasmids The Human TFome Library Stem Cells Protocols & References MORF Plasmids MORF Pooled...out PCR for 10X scRNA-seq (PDF, 59.2 KB) Plasmids ID Plasmid Gene/Insert Pooled Library The MORF pooled...suggestions for other plasmids that should be added to this list? Fill out our Suggest a Plasmid form or e-mail...

  3. CRISPR Pooled gRNA Libraries

    Type
    Collection
    ...Broad GPP genome-wide Brunello 73179 (1 plasmid) 73178 (2 plasmid) Knockout Human Doench and Root 3rd 4 ...76,441 Broad GPP genome-wide Brie 73632 (1 plasmid) 73633 (2 plasmid) Knockout Mouse Doench and Root 3rd 4 ...GPP kinome Brunello 75314, 75315 (1 plasmid) 75312, 75313 (2 plasmid) Knockout Human Doench and Root 3rd... 3,052 Broad GPP kinome Brie 75317 (1 plasmid) 75316 (2 plasmid) Knockout Mouse Doench and Root 3rd 4 ...20,051 Human GeCKO v2 1000000048 (1 plasmid) 1000000049 (2 plasmid) Knockout Human Zhang 3rd 6 123,411...185,836 Mouse GeCKO v2 1000000052 (1 plasmid) 1000000053 (2 plasmid) Knockout Mouse Zhang 3rd 6 130,209... - 3 plasmid system 1000000075 (Puromycin) Activation Mouse Zhang 3rd 3 69,716 SAM v2 - 2 plasmid system...

  4. Zinc Finger Consortium Reagents

    Type
    Collection
    ...like... Keith Joung Lab plasmids Daniel Voytas Lab plasmids Scot Wolfe Lab plasmids The Zinc Finger Consortium... Plasmid Collections Zinc Finger Consortium Zinc Finger ...Technologies TALEN Engineering Reagents CRISPR Plasmids...

  5. Malate Dehydrogenase CUREs Community Collection

    Type
    Collection
    ...download from each plasmid page. Use the search bar or sort buttons to find plasmids based on: Organism...collaborations between institutions. With these plasmids, faculty can begin a sustainable MDH-related CURE...and metabolic pathways and protein interactions. Plasmids A diverse set of wild-type MDH genes from several...the C-terminus of MDH and, if indicated on the plasmid map, a TEV cleavage site is present between the..."active site loop", or "subunit interface") ID Plasmid Description Gene/Insert Mutations Additional Resources...opens in a new window) Acknowledgments The MCC and plasmid construction was funded by NSF IUSE 1726932 and...

  6. Deisseroth INTRSECT Collection

    Type
    Collection
    ...Collections INTRSECT Plasmid Collection INTRSECT Plasmid Collection INTRSECT Implementation Plasmids References ...novel INTSRECT plasmids for groups interested in producing their own INTRSECT plasmids. Online spreadsheet...Find intersectional expression plasmids that combine multiplexed recombinases, including Cre, Flp, and...that have used these constructs experimentally. Plasmids In addition to EYFP and ChR2-EYFP, a large number...rabies targeting genes. Recombinases Addgene ID Plasmid Logic Sites and Mutations In-Stock Viral Items ...None Yes Single recombinase-dependent Addgene ID Plasmid Logic Sites and Mutations In-Stock Viral Items ...recombinase-dependent: Fluorophores Addgene ID Plasmid Logic Sites and Mutations In-Stock Viral Items ...

  7. Antibodies

    Type
    Collection
    ...Looking for Antibody Plasmids? Browse our collection of affinity reagent plasmids - including antibodies...affinity reagents are expressed and purified from plasmids in our repository. Antibodies...collection of affinity reagents that are expressed from plasmids. These antibodies undergo application-specific...antibodies, nanobodies, scFvs, and more! Find Plasmids...

  8. Rett Syndrome

    Type
    Collection
    ...Rudolf Jaenisch labs Plasmids MECP2 Plasmids The table below highlights plasmids that express or target...information on generating iPSCs using plasmids, please see Addgene's Plasmids for Stem Cell Research page. CRISPR...Broswe plasmids and other research tools for use in Rett Syndrome research. Plasmid... Plasmid Collections Rett ...Rett Syndrome Rett Syndrome Plasmid Collection and Resource Center MECP2 Animal Models Human Cell Lines CRISPR... CRISPR Tools Plasmids Resources The (Link opens in a new window) Rett Syndrome Research Trust (RSRT) ...individual laboratories to assemble a Rett Syndrome plasmid resource for the scientific research community....

  9. Viral Production

    Type
    Collection
    ...the transfer plasmid, a plasmid encoding Rep and serotype-specific Cap genes, and a plasmid encoding adenoviral... coli . In addition, plasmids are typically prepared using endotoxin-free plasmid purification kits. Resources...contamination is minimized by using an endotoxin-free plasmid purification protocol. To minimize the immunogenic...off-line and discarded. Confirmation of Transfer Plasmid Addgene uses a rigorous barcode matching system...microbial contamination. Purity When possible, all plasmids used for viral production are propagated in the...experiment using our virus protocols . Browse our plasmid collection of popular lentiviral vectors ....

  10. CRISPR References and Information

    Type
    Collection
    ...Addgene CRISPR Resources CRISPR Plasmids Addgene's collection of CRISPR plasmids. CRISPR Guide Addgene's guide...guide to using CRISPR plasmids in your lab, from knockouts to pooled library screening. CRISPR eBook Addgene's...barcoding. CRISPR Kits Addgene's collection of CRISPR plasmid kits. CRISPR Software Sanger Indel Analysis ICE... Feng Zhang Lab . Protocols Lab(s) Description Plasmids in protocol Download protocol Addgene CRISPR pooled...gRNA design and cloning for Cas9 orthologs Cas9 plasmids PDF, 107 KB Chen and Wente Zebrafish: gRNA cloning... (Cre recombinase) PDF, 105 KB Jaenisch CRISPR plasmid summary for transcriptional activation dCas9 activators... proteins pFETCh_Donor ; additional HDR & gRNA plasmids are available for CREB1, ATF1, GABPA, and RAD21...

  11. Serotype Testing AAV

    Type
    Collection
    ...AAV Vectors for Serotype Testing pAAV-CAG-GFP (Plasmid #37825) Description : Ready-to-use AAV in various... for direct injection. These AAV are made from plasmid 37825 (deposited by Edward Boyden ) and direct ...AAVrg.T 20 µL $ 150 Add to Cart pAAV-hSyn-EGFP (Plasmid #50465) Description : Ready-to-use AAV in various... for direct injection. These AAV are made from plasmid 50465 (deposited by Bryan Roth ) and direct EGFP...a sample of the purified DNA from the original plasmid that was used to make the virus. For more details...options. Select from a vast catalog of eligible AAV plasmids and we’ll make the viral vector prep you need....

  12. mTOR Pathway

    Type
    Collection
    ...proliferation. mTOR Pathway Plasmids Click on a name to find available plasmids for the gene, or browse the...suggestions for other plasmids that should be added to this list? Fill out our Suggest a Plasmid form or e-mail...Addgene's plasmids for the mTOR pathway. enhanced signaling of mTOR, a key metabolic regulator, correlates...with a New Screening Kit Background mTOR Pathway Plasmids mTOR Gene List Resources The m echanistic or m... - Gene List Click on a name to find available plasmids for the gene. For components with multiple isoforms...

  13. Fluorescent Proteins: FRET

    Type
    Collection
    ...backbones and control plasmids. Donor Acceptor λ Dex QY D λ Aem ε A QY A R 0 J(λ) Plasmids mTagBFP sfGFP 399.... Browse Addgene's Luciferase Plasmid Collection or the Promega Plasmid Collection to find related tools...Find plasmids and resources for FRET experiments to measure protein interactions or conformation changes... Plasmid Collections Fluorescent Proteins FRET Fluorescent Proteins: FRET FRET Pairs FRET Calibration...FRET on our blog and explore the tables below for plasmids and information to help plan your next FRET experiment...The articles or collections listed below feature plasmids with positive and negative FRET controls or calibration... the cpFRET kit from the Pertz Lab or the 2in1 Plasmid Toolkit from the Grefen Lab. FRET pairs and biosensors...

  14. Impact of Genomic Variation on Function (IGVF) Consortium Collection

    Type
    Collection
    ...and phenotype and shares their plasmid tools through Addgene. ID Plasmid Description Vector Type PI Publication...Browse plasmid tools generated by IGVF Consortium investigators to examine how genomic variation affects...genomic variants on genome function and phenotypes. Plasmids The collection will grow as the IGVF community...

  15. Lentiviral Prep Service

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    Collection
    ...are made from plasmids that are currently in our repository. You can obtain the plasmid from us or receive... list of gRNA plasmids targeting specific genes. For more information on CRISPR plasmids and function,...processes. For more information, see our Guide to Plasmid Pooled Libraries . ID Name Description Genes/Insert...function, see our CRISPR plasmids resource page . Cas9 for Editing Genomic Sequences To perform genome edits,... Browse our list of available viruses based on plasmids in our repository that express fluorescent proteins...

  16. Fluorescent Protein Guide: Empty Backbones

    Type
    Collection
    ...Neveu Lab MXS Chaining Kit Luciferase Plasmid Collection Promega Plasmid Collection Reading and Resources ...suggestions for other plasmids that should be added to this list? Fill out our Suggest a Plasmid form or e-mail...Explore empty plasmid backbones with different fluorescent tags to create fusion proteins with your gene... Plasmid Collections Fluorescent Proteins Empty Backbones Fluorescent Proteins: Empty...Emission (nm) Brightness pKa Maturation Structure Plasmids Sirius 355 424 4 3.0 Prone to dimerization Sirius-N1...Emission (nm) Brightness pKa Maturation Structure Plasmids Aquamarine 420 474 23 3.3 Prone to dimerization...Emission (nm) Brightness pKa Maturation Structure Plasmids AcGFP1 475 505 28 Prone to dimerization AcGFP1...

  17. p53 Pathway

    Type
    Collection
    ...function in cancer. p53 Pathway Plasmids Click on a name to find available plasmids for the gene, or browse the...suggestions for other plasmids that should be added to this list? Fill out our Suggest a Plasmid form or e-mail...Addgene's collection of plasmids for the p53 pathway. p53 is a transcription factor and tumor suppressor...mTOR pathway Ras pathway Background p53 Pathway Plasmids p53 Gene List References Background p53 is a transcription... - Gene List Click on a name to find available plasmids for the gene. For components with multiple isoforms...

  18. Kazuhiro Oka Lentiviral Vectors

    Type
    Collection
    ...Vector Guide Find more plasmids in our Lentiviral Plasmids and Adenoviral Plasmids collections Addgene Blog...reporter plasmids to test whether or not you've efficiently generated infectious virus, plasmids expressing... Blog: Your Lentiviral Plasmid FAQs Biosafety Resource Guide Content last reviewed: 15 August 2025...

  19. All Antibodies

    Type
    Collection
    ...provide important details including the protein and plasmid sequence, the antigen sequence, and cross-species...develop and refine these application lists. The plasmids we use to produce antibodies are available as ...as part of Addgene’s larger Antibody Plasmid Collection , which also includes other types of affinity reagents...? Check out our current collection of Antibody Plasmids , fill out our Suggest an Antibody form, or email...

  20. Plan Your Experiment

    Type
    Collection
    ...design. Addgene offers many plasmids containing validated gRNAs . These plasmids contain gRNAs that have ...generate stable cell lines if your plasmid contains a selection marker. Plasmid delivery is versatile thanks...Application Cas Enzyme Additional Considerations Browse Plasmids Knockout Permanently disrupt gene function; cells...different activator systems exist, including the multi-plasmid SAM system Activate Design and Clone Your gRNA ..., anneal, and insert the targeting oligos into plasmids containing the gRNA scaffold using standard restriction-ligation...review the protocol associated with the specific plasmid in question (see CRISPR protocols from Addgene ...the expression time (transient or stable). For plasmid and viral-based approaches, the species and expression...
Showing: 101 - 120 of 155 results