...also like... Keith Joung Lab plasmids Dan Voytas Lab plasmids CRISPR plasmids The Joung Lab has developed...Gene Additional Plasmids compatible with the REAL Assembly TALEN Kit: TALE-LSD1 plasmids for epigenome ... simplify the construction of TALEN expression plasmids using REAL, REAL-Fast or FLASH assembly. This ...REAL or REAL-Fast assembly process or lists of plasmid ID numbers required for FLASH assembly. The software...Assembly TALEN Kit Individual REAL TALE Repeat Plasmids TALEN Expression Vectors for REAL, REAL-Fast and...

...below for CRISPR plasmids for RNA editing in mammalian systems. Mammalian ID Plasmid Gene/Insert PI Publication...suggestions for other plasmids that should be added to this list? Fill out our Suggest a Plasmid form or e-mail...CRISPR plasmids based on Cas13 designed to precisely edit RNA targets. CRISPR...Plasmids: RNA Editing Browse CRISPR Plasmids By Function Genome Editing Cut Base Edit Nick Prime Edit Transcriptional...

...BioBricks Foundation Plasmids Drew Endy Lab Plasmids Jennifer Molloy Lab Plasmids Golden Gate cloning ...Golden Gate assembly). Plasmids Explore the collection and order individual plasmids via the links below:...Browse the Open Enzyme collection of plasmids at Addgene for generating enzymes for molecular biology... Lab's Open Enzyme Collection . How to Use The plasmids in the Open Enzyme collection contain genetic ...functional by themselves. The selection marker for all plasmids in this collection is ampicillin. Find more information...Polymerases Other Enzymes DNA Ligases Addgene ID Plasmid Gene/insert 165503 pOpen-HSlig4 DNA Ligase IV 165514...Ligase III Return to top DNA Polymerases Addgene ID Plasmid Gene/insert 165500 pOpen-9N7polA 9 degrees N-7 ...

...PMID 23287722 Individual plasmids can be ordered via the links below: ID Plasmid Description 41824 gRNA ...Cas9 expression plasmid 41816 hCas9 D10A A human codon-optimized Cas9 expression plasmid mutated to nick...PMID 23460208 Individual plasmids can be ordered via the links below: ID Plasmid Description 43802 p414-...PMID 24076762 Individual plasmids can be ordered via the links below: ID Plasmid Description 48669 M-ST1cas...CRISPR Cas9 gRNA plasmids from Dr. George Church lab. CRISPR... CRISPR Church Lab CRISPR Plasmids Church Lab CRISPR...CRISPR Plasmids Available from Addgene You may also like... CRISPR Guide CRISPR Protocols gRNA Design Tools...

...scheme Fujii Lab Plasmids Individual plasmids can be ordered via the links below: ID Plasmid Description 47948...elements/pMD20 iChIP plasmid, LexA-binding elements 48874 3xFNLDD/pCMV-7.1 iChIP plasmid, expresses 3xFNLDD...-I2 iChIP plasmid, expresses 3xFNLDD in mammalian cells 48972 3xFNLDD/pMXs-IG iChIP plasmid, expresses... gRNA plasmids from Dr. Hodaka Fujii's lab. Genome... Genome Engineering CRISPR Fujii Lab CRISPR Plasmids Fujii ...Fujii Lab CRISPR Plasmids Available from Addgene You may also like... CRISPR Guide CRISPR Protocols gRNA Design...information and protocols, check our references for the plasmids or browse the Fujii Lab web page . Figure 1: iChIP...

...joined here ). Individual plasmids can be ordered via the links below: ID Plasmid 47511 pFYF1320 EGFP Site... Lab Guide RNA Expression Plasmids for EGFP CRISPR... CRISPR Joung Lab CRISPR Plasmids EGFP gRNA CRISPR-Cas/RGN ...RGN expression plasmids for EGFP You may also like... CRISPR Guide CRISPR Protocols gRNA Design Tools CRISPR... et al., Nat Biotechnol. 2013). The expression plasmids encoding the guide RNAs ( gRNAs ) used in these...

... and plasmid collections that may help with your neurodegeneration research. Disease Info Plasmid Collection...Research Tools Neurodegeneration Plasmid Collection A collection of plasmids expressing wild type or mutant...Subcellular Localization, Optogenetics Plasmids, and Chemogenetics Plasmids. New and Noteworthy: Explore NeuroPAL...more. Plasmids provided by the foundation can be found on our Michael J. Fox Foundation Plasmid Resource...Find out about the plasmid collections available at Addgene that may be useful for neurodegeneration ...genes linked to neurodegenerative disorders. Find plasmids expressing HTT, C9ORF72, LRRK2, Synuclein, and... species. You can also find our guide to using plasmid pooled libraries . New and Noteworthy: Use a CRISPRi...

...Biotechnol. 2014. Individual plasmids can be ordered via the links below: ID Plasmid 53370 pSQT1313 (multiplex... CRISPR Joung Lab CRISPR Plasmids CRISPR-FokI Dimeric CRISPR RNA-guided FokI Nuclease...Design Tools CRISPR Blog Posts This page lists plasmids for practicing the dimeric CRISPR RNA-guided FokI... and a FokI-dCas9 fusion protein. The pSQT1313 plasmid enables the expression of two gRNAs in a single...Note that the FokI-dCas9 fusion encoded by this plasmid is the one that showed optimal activity in human...multiplex gRNA expression plasmid) 53369 pSQT1601 (optimized Csy4 and FokI-dCas9 expression vector)...

... 24112467 . Individual plasmids can be ordered via the links below: ID Plasmid 46965 pK7WGF2::hCas9 : ...CRISPR Cas9 gRNA plasmids from Dr. Sophien Kamoun's lab. CRISPR... CRISPR Kamoun Lab CRISPR Plasmids Kamoun Lab CRISPR...CRISPR Plasmids Available from Addgene You may also like... CRISPR Guide CRISPR Protocols gRNA Design Tools...protocol on assembling customised sgRNA expressing plasmids. Questions regarding the protocol can be addressed...tsl.ac.uk or [email protected] . These plasmids are described in: Targeted mutagenesis in the ...

...joined here ). Individual plasmids can be ordered via the links below: ID Plasmid 47505 pVC297 VEGF Site#1...Guide RNA Expression Plasmids for Endogenous Human Genes CRISPR... CRISPR Joung Lab CRISPR Plasmids Human gRNA CRISPR-Cas...CRISPR-Cas/RGN expression plasmids for Human genes You may also like... CRISPR Guide CRISPR Protocols gRNA... et al., Nat Biotechnol. 2013). The expression plasmids encoding the guide RNAs ( gRNAs ) used in these...

...Browse Antibody Plasmids The following plasmids can be used to create or express plasmid-based antibodies...epitope tags , as well as antibody plasmids to create or express plasmid-based antibodies. The majority of...Integrin Antibodies Epitope Tag Antibodies Antibody Plasmids Welcome to the Institute for Protein Innovation...antibodies. ID Plasmid Description...

...Broad GPP genome-wide Brunello 73179 (1 plasmid) 73178 (2 plasmid) Knockout Human Doench and Root 3rd 4 ...76,441 Broad GPP genome-wide Brie 73632 (1 plasmid) 73633 (2 plasmid) Knockout Mouse Doench and Root 3rd 4 ...GPP kinome Brunello 75314, 75315 (1 plasmid) 75312, 75313 (2 plasmid) Knockout Human Doench and Root 3rd... 3,052 Broad GPP kinome Brie 75317 (1 plasmid) 75316 (2 plasmid) Knockout Mouse Doench and Root 3rd 4 ...20,051 Human GeCKO v2 1000000048 (1 plasmid) 1000000049 (2 plasmid) Knockout Human Zhang 3rd 6 123,411...185,836 Mouse GeCKO v2 1000000052 (1 plasmid) 1000000053 (2 plasmid) Knockout Mouse Zhang 3rd 6 130,209... - 3 plasmid system 1000000075 (Puromycin) Activation Mouse Zhang 3rd 3 69,716 SAM v2 - 2 plasmid system...

...suggestions for other plasmids that should be added to this list? Fill out our Suggest a Plasmid form or e-mail...expression plasmids that have been designed for use in parasites. CRISPR... CRISPR Parasites CRISPR Plasmids: Parasites...Parasites Browse CRISPR Plasmids By Function Genome Editing Cut Base Edit Nick Prime Edit Transcriptional ...development. This collection brings together some of the plasmids that have been used in these efforts. The content...HDR occurs at a much lower efficiency than NHEJ. Plasmid Gene/Insert Selectable Marker PI Publication Libraries...

...the right to use the original plasmid. Distribution of the original plasmid is important because it is the...Overview of Addgene's viral preparation service for plasmids and pooled libraries. Viral preparation options... produces ready-to-use viral preps from select plasmids in our collection. We know that viral vector production... production is a common step after requesting plasmids from Addgene, and by providing this service, we...a sample of the purified DNA from the original plasmid that was used to make the virus. In order for Addgene...inclusion is what gives you permission to use the plasmid....

...joined here ). Individual plasmids can be ordered via the links below: ID Plasmid 42251 MLM3613 (Cas9 expression...RNA Expression Plasmids for Endogenous Zebrafish Genes CRISPR... CRISPR Joung Lab CRISPR Plasmids Zebrafish gRNA...gRNA CRISPR-Cas/RGN expression plasmids for Zebrafish You may also like... CRISPR Guide CRISPR Protocols... et al., Nat Biotechnol. 2013). The expression plasmids encoding the guide RNAs ( gRNAs ) used in these...

...Keith Joung Lab plasmids Daniel Voytas Lab plasmids CRISPR plasmids This page lists plasmid vectors into ...with the Joung Lab Individual REAL TALE Repeat Plasmids in the Joung Lab REAL Assembly TALEN Kit . Vectors...domains ( Sander et al., Nat Biotechnol. 2011 ): ID Plasmid C-terminal 0.5 TALE repeat Binds 32285 JDS70 NI...

... may also like... Zhang Lab CRISPR plasmids General CRISPR Plasmids Dr. Le Cong, the first author of one...easiest way is to co-transfect several plasmid together, with each plasmid having a targeting spacer cloned ... Genome Engineering CRISPR Zhang Lab CRISPR Plasmids Zhang Lab CRISPR FAQs Zhang Lab's CRISPR Frequently...with expression, though it is possible for the plasmid to still express without the G. Because the G is...experiments for multiplexed targeting with Zhang lab plasmids? For multiplexing CRISPR to target multiple genome...into PX330, then co-transfect or delivery the two plasmids together into your cells. If you want to use double...very big concern, you could go ahead with PX330 plasmid, clone in your target guides, test run them and...

... Plasmid Collections Chemogenetics Plasmids Chemogenetics Plasmid Collection You may...Chemogenetic Plasmids This table contains Addgene's collection of chemogenetic plasmids plasmids. The table...receptor type, plasmid type, promoter, reporter or fusion protein, and whether the plasmid is Cre- or Flp...found. Browse all DREADD plasmids Do you have suggestions for other plasmids that should be added to this...Chemogenetics vs. Optogenetics Background Chemogenetics plasmids encode genetically engineered receptors that are...ready-to-use AAV preparations of select chemogenetic plasmids; these are noted with the viral service icon ....IRES-EGFP Recombinase Cre-dependent Flp-dependent Plasmid type AAV Mammalian expression, non-viral Viral ...

...component to find plasmids with your gene of interest. Browse Cancer Research Plasmids by Pathway mTOR ...Addgene's curated collection of plasmids for cancer research, organized by signaling pathway. Special...Special Collections Cancer Research Cancer Research Plasmids and Resources Addgene has a large variety of resources...Each collection page listed below organizes the plasmids and resources by signaling pathway, focusing on...Libraries for Cancer Research Broad Target Accelerator Plasmids: Collection of vectors from the Boehm , Hahn ,...

... Zhang Lab Plasmids The Human TFome Library Stem Cells Protocols & References MORF Plasmids MORF Pooled...dial out PCR for 10X scRNA-seq (59.2 KB) Plasmids ID Plasmid Gene/Insert Pooled Library The Multiplexed...suggestions for other plasmids that should be added to this list? Fill out our Suggest a Plasmid form or e-mail...