...added to this list? Fill out our Suggest a Plasmid form or e-mail [email protected] to help us improve this... is mutation is noted when present. For more information on the properties of many of these proteins, ...

...added to this list? Fill out our Suggest a Plasmid form or e-mail [email protected] to help us improve this...also listed here and denoted as such. Want more information on the wide variety of Cas enzymes? CasPEDIA ...

...added to this list? Fill out our Suggest a Plasmid form or e-mail [email protected] to help us improve this...switch a powerful experimental tool. For more information on FLEx switch and what you can do with it read...technology. Read the genOway press release for more information. Empty backbones to regulate your gene of interest...

...added to this list? Fill out our Suggest a Plasmid form or e-mail [email protected] to help us improve this... High-performance GFP-based calcium indicators (Constitutive or Cre-dependent) High-performance calcium...specific molecules or other factors. Most often, a conformational change in the sensing region of the construct...Citron/Citroff), mitochondrial or cytosolic High-Performance Intensiometric Direct- and Inverse-Response Genetically...

...subunit B, beta isoform PPP3RL PRF1 perforin 1 (pore forming protein) FLH2, HPLH2, MGC65093, P1, PFN1,...added to this list? Fill out our Suggest a Plasmid form or e-mail [email protected] to help us improve this...phosphatase 3 (formerly 2B), catalytic subunit, gamma isoform CALNA3 PPP3R1 protein phosphatase 3 (formerly 2B),...PPP3CA protein phosphatase 3 (formerly 2B), catalytic subunit, alpha isoform CALN, CALNA, CALNA1, CCN1, ...PPP3CB protein phosphatase 3 (formerly 2B), catalytic subunit, beta isoform CALNA2, CALNB PPP3CC protein...

...protocols and performing rigorous quality control (QC). The specific QC experiments performed varies for ...Link opens in a new window) . Transfections are performed using the transfer plasmid, a plasmid encoding...and stored at -80 °C. Titer Titering is either performed by Addgene or by the University of Pennsylvania...Pennsylvania Vector Core. In general, titering is performed by the facility that produced the viral vector lot....Pennsylvania Vector Core is (as of April 2016) also performed by droplet digital PCR (ddPCR). Quality Control.... To learn which specific QC experiments were performed on your lot, please contact us . Full sequencing...the Viral Genome Next-generation sequencing is performed on viral genomes isolated from the final AAV preparation...

...methods, and quality control are described below. Information for a specific antibody can be found on the antibody's...reviewing the specific antibody webpage for further information. Production For recombinant antibodies (rAb) ...using Protein A or Protein G affinity columns. Formulation Buffer After affinity chromatography, antibodies...exchange using centrifugal columns. The final formulation buffer is phosphate-buffered saline containing...recommended applications. The specific QC experiments performed varies for each antibody catalog item. Whenever...Whenever possible, the experiment is performed in parallel with a previous lot of recombinant antibody or the.... To learn which specific QC experiments were performed on your lot, please email [email protected] . Immunocytochemistry...

...of your chosen AAV plasmid for more information on its performance. ID Plasmid Gene/Insert Promoter PI... can do this whenever you are ready. Payment information must be added before we can begin processing ...this time, we amplify DNA, produce the prep, and perform comprehensive quality control processes to make...Shipping 2–7 days We will email you with tracking information as soon as your order ships. Shipping time will...whether the item can be packaged. Addgene does not perform functional or in vivo testing. We recommend that...Addgene’s Help Center, a searchable database of information to help you every step of the way, including ...

...bio 73098 CD44-isoform1-CD4d3+4-bio 73099 CR1-isoformB-CD4d3+4-bio 73100 Basigin-isoform1-CD4d3+4-bio 73101...Galectin3-CD4d3+4-bio 73129 Basigin-isoform2-CD4d3+4-bio 73130 CD44-isoform12-CD4d3+4-bio... Please see the following reference for more information: Basigin is a receptor essential for erythrocyte...

...literature information (Link opens in a new window) OMIM MECP2 - MECP2 curated literature information (Link...which they were created. Find their contact information by following the link to their laboratory website...Link opens in a new window) Wendy Gold For more information on generating iPSCs using plasmids, please see...MECP2 - MECP2 curated protein and functional information Mouse and Cell Line Repositories (Link opens ...Brain - includes developmental transcriptome information, prenatal LMD microarray data, ISH image data...

...MDH. For detailed clone information, please see the MDH Protein Clone Information Sheet, available to download...window) and contact the hub director for more information. Why MDH? MDH is stable, inexpensive to assay...techniques. Using a single protein with many available isoforms and mutants provides a shared experience for the...mentor, and more. References for teachers Using Bioinformatics and Molecular Visualization to Develop Student...

...into an expression vector of their choice and transforming into E. coli bacteria. This can help reduce ...genetic engineering and directed evolution. More information about the collection can be obtained at the Open...an expression vector of their choice and then transform into E. coli for expression and purification (... in this collection is ampicillin. Find more information on the enzymes and protocols for expression in...Uracil DNA glycosylase 165541 pOpen-Fpg Fpg Formamidopyrimidine DNA glycosylase 165550 pOpen-T4 PGD T4 PGD...

...and resources are up-to-date with reagents and information you need to use CRISPR in your own lab. Check...Resources CRISPR Guide : Essential background information on CRISPR and the basics for planning your first...to reverse transcriptase install new genetic information into a specific DNA site. Base Edit Base editors...editors precisely edit DNA without promoting DSB formation. Delete NEW Cascade-Cas3 generates large genomic...

...Packaged on Request Viral Vector Packaging Service Information Production Our production process and quality...viral vector production at Addgene, ordering information, and more. Additional Viral Vector Resources ...from the research community demonstrating AAV performance under various experimental conditions. Protocols...institutional biosafety office and your lab, information on the biosafety of viral vectors, and general...

... Collection Technology Plasmids Supplemental Information New England Biolabs offers non-toxic protein ...labeling in a sample. Please see the note below for information on substrates and synthases needed for use of...Control for cell surface localization Supplemental Information SNAP and CLIP tags SNAP and CLIP tag FAQs (PDF... Bergendahl V, Marahiel MA.1998. Peptide bond formation in nonribosomal peptide biosynthesis. Catalytic...

...genetic information, viruses are a powerful tool for the delivery of this genetic information to cells.... Viruses facilitate the delivery of genetic information to hard-to-transfect mammalian cells. In addition...of different types of viruses. More detailed information can be found in the guides for each virus type...

...CRISPR plasmids The Joung Lab has developed three platforms for engineering TAL effector repeat arrays of ...al., Nat Biotechnol 2012). All three of these platforms assemble TAL effector repeat arrays on a single...Maeder et al., Nat Methods 2013 ) More detailed information about REAL, REAL-Fast and FLASH, including detailed...

...been cloned, expressed and validated in recombinant form. The recombinant monoclonal antibodies or R-mAbs...IgG2a 149452 Anti-Kirrel3, short isoform 3 [N320/48R] Kirrel3, short isoform 3 Mouse Mouse IgG2a 149453 Anti-Kv4.2...IgG2a 188208 Anti-FGF13/FHF2, B isoform [N225A/10R] FGF13/FHF2, B isoform Human Mouse IgG2a 188209 Anti-...190541 FGF13/FHF2, B isoform scFv [N225A/10] N225A/10 scFv FGF13/FHF2, B isoform Human Mouse 190542 Pan-Shank...

...Please see the following references for more information: Large-scale screening for novel low-affinity...18296487 A benchmarked protein microarray-based platform for the identification of novel low-affinity extracellular...

...Please see the following references for more information: Large-scale screening for novel low-affinity...Integrated Expression) an online database of information on protein interactions generated using AVEXIS...