...gRNA sequence to direct the dCas9-activator to promoter or regulatory regions of your gene of interest....-FokI Screen Pooled Libraries gRNAs Empty gRNA Vectors gRNAs By Species Mammalian Bacteria Drosophila ...Catalytically dead dCas9 fused to a transcriptional activator peptide can increase transcription of a specific...separate gRNA expression plasmid to target the dCas9-activator to your specific locus. Want more information ...

...Plasmid Gene/Insert Vector Type Promoter PI Publication Empty gRNA Expression Vectors Select a gRNA expression...-FokI Screen Pooled Libraries gRNAs Empty gRNA Vectors gRNAs By Species Mammalian Bacteria Drosophila ...expression plasmid based on factors such as selectable marker or cloning method. When using CRISPR, you...both the gRNA and Cas protein act as all-in-one vectors, but their function is often limited to a single...

...plasmids to study the regulatory networks involved in retinal development.... Depositor Collections Brzezinski...mouse retina. To aide in their studies of the regulatory networks involved in retinal development the ...Prdm1 and Vsx2 enhancers in the retina alters photoreceptor and bipolar cell fate specification, yet differs...

... Viral Vector Packaging Service AAV Biosensors Viral Vector Packaging Service: Biosensor...CAG CaMKIIa Dlx EF1a GFAP/GfaABC1D Synapsin E2 regulatory element Activity Cre-dependent Flp-dependent ...Karpova and Schaffer labs. See the Retrograde AAV Inventory Page . Don’t See What You’re Looking For? Our ... eligible AAV plasmids and we’ll make the viral vector prep you need. Or fill out our form to let us know...format. Please note this does not guarantee viral vector packaging service, but lets us know what viruses...

... Depositor Collections FreeGenes Project Open Enzyme Collection... own enzymes by cloning them into an expression vector of their choice and transforming into E. coli bacteria...Researchers should clone these enzymes into an expression vector of their choice and then transform into E. coli...expression and purification ( pET28a-LIC or other pET vectors are recommended). The selection marker for all ...Additional Resources Bacterial Protein Expression Vectors BioBricks Foundation Plasmids Drew Endy Lab Plasmids...

...will aid neuroscientists in studying circuits in laboratory animals and human tissue specimens. These technologies...rAAV-hI56i-minBglobin-iCre-4X2C-WPRE3-BGHpA AAV vector for Cre expression in forebrain GABAergic interneurons...lines or co-infection with Cre dependent viral vectors. Jonathan Ting Viviana Gradinaru 163509-PHPeB CN1839...CN1839-rAAV-hSyn1-SYFP2-10aa-H2B-WPRE3-BGHpA AAV vector for nuclear SYFP2 labeling in neurons. Useful for...BGHpA DLX2.0 (3xCore version of DLX enhancer). AAV vector for strong & rapid SYFP2 expression in forebrain...

... Depositor Collections Rinehart Lab Phosphoprotein Reagents...This library is anticipated to be useful for laboratories performing phosphoproteomic experiments in need...can also be introduced into a second expression vector encoding a split mCherry fluorescent reporter, ...domain and the phosphosite, mCherry fluorescence is restored, so interactions can be discovered by fluorescence-activated... via partial UAG codon reassignment and release factor 1 deletion. Heinemann IU, Rovner AJ, Aerni HR, ...

...Protein Collection . Return to top Secretory Pathway The secretory pathway is named for its role in secreting...Posts Background General Cytoskeletal Junctions Secretory Pathway Background The subcellular localization... shipped. Many of the proteins that enter the secretory pathway require chaperones to help with folding... or search the table below to find markers of secretory pathway components. ID Plasmid Structure Targeting... 24920 pEGFP-LC3 (human) Autophagosome LC3 EGFP Toren Finkel 14435 mRFP-Clc Clathrin-coated vesicles Clathrin...

...basic-hFX-promotor(1kb) FXN hFXN Friedreich ataxia Michael Ristow 14979 pGL3-basic-hFX-promotor(1,3kb) FXN...basic-hFX-promotor(2,5kb) FXN hFXN Friedreich ataxia Michael Ristow 14981 pGL3-basic-hFX-promotor(2kb, distal...TARDBP His, GST, Factor Xa cspA ALS Aaron Gitler 27452 pCold TDP43 G294A TARDBP His, Factor Xa cspA ALS Aaron...M337V TARDBP His, Factor Xa cspA ALS Aaron Gitler 27454 pCold TDP43 Q331K TARDBP His, Factor Xa cspA ALS Aaron...19 James Trimmer 129409 Slc1a3-CreERT2 Targeting Vector SLC1A3 PGK Episodic ataxia Walker Jackson 129410...pET3d-SF-DCTN1-TS DCTN1 SplitFIAsH, Strep, His T7 ALS, motor neuron disease Bernd Bukau 169713 A1-LCD WT HNRNPA1...107R] VAPB CMV ALS James Trimmer 188167 Anti-IP3 receptor, type 1 [L24/21R] ITPR1 CMV Spinocerebellar ataxia...

... Depositor Collections Cell Migration Consortium (CMC) Plasmids...Cell Migration Consortium and Addgene. Member laboratories of the Cell Migration Consortium have deposited...for cell migration research at Addgene. CMC investigators who have deposited plasmids include: Joan Brugge...Francisco Browse Plasmids ID Plasmid Gene/Insert Vector Type Backbone Mutations Tags PI...

... plasmid tools generated by IGVF Consortium investigators to examine how genomic variation affects genome... Depositor Collections IGVF Consortium Impact of Genomic Variation...collaborative program that brings together teams of investigators who will use state-of-the-art experimental and...plasmid tools through Addgene. ID Plasmid Description Vector Type PI Publication Additional Resources The IGVF...

...Human erythrocyte receptor proteins for use with AVEXIS interaction screening...Platelet EXPRESs Plasmids for Human Erythrocyte Receptors You may also like... Gavin Wright Lab plasmids...extracellular protein interactions to identify novel receptor-ligand interactions that function in the invasion...following reference for more information: Basigin is a receptor essential for erythrocyte invasion by Plasmodium...

...Empty gRNA Expression Vectors Select a gRNA expression plasmid based on factors such as selectable marker...-FokI Screen Pooled Libraries gRNAs Empty gRNA Vectors gRNAs By Species Mammalian Bacteria Drosophila ...both the gRNA and Cas protein act as all-in-one vectors, but their function is often limited to a single...

...-FokI Screen Pooled Libraries gRNAs Empty gRNA Vectors gRNAs By Species Mammalian Bacteria Drosophila ...fragments of DNA at specific locations. The Cas effector lacks nuclease activity, instead recruiting the... and type V-K. Type I systems use a type I Cas effector, composed of several separate Cas enzymes that...orientation matters. Type V-K systems use a type V-K Cas effector, Cas12k, and three transposase components (TnsB...

...is a Monomer not a Monomer? Addgene's plasmid repository contains a variety of fluorescent protein plasmids...conformational changes within a protein. Biosensors Monitor small biomolecules or other physiological intracellular...signals, cells, or groups of cells with either actuators or sensors. Chemogenetics Plasmids | Guide Use...molecules to activate genetically engineered cellular receptors that affect signalling pathways within cells. ...

...any viral vector. Addgene performs QC measures which verify the identity of the viral vector, but not ... Viral Vector Packaging Service Packaged on Request Scope of Service AAV Packaged on Request: Scope of...guarantees above. We will have our expert viral vector production team carefully review your cargo/serotype...

...antibodies (rAb) encoded by mammalian expression vectors, human embryonic kidney cells are transfected with...Neuromab Protocols (Link opens in a new window) page. Storage and Shipping Stability Addgene does not recommend... suggests preparing single-use aliquots before storing at -20 °C. Each antibody catalog item is tested...some of our other resources to help you choose, store, or use your antibody. Antibody Guide Antibody Protocols...

...s reverse complement for cloning into the pX330 vector using BbsI restriction enzyme ( Table 3 ). Add ...sgRNA expression from the U6 promoter of the pX330 vector is enhanced by the inclusion of a G nucleotide ...Prepare a 50 μl reaction mix: 100 ng circular pX330 vector, 1.0 μl annealed oligos (1 μM; see step 3.1.4 )... ( e.g., with a PCR cloning kit) into a plasmid vector. Transform the plasmid into DH5α E. coli cells ...

...Modular Assembly method Nuclease Expression Vectors - Cloning vectors for expressing zinc finger FokI nuclease...proteins for distribution to academic and nonprofit laboratories for research and educational use. Links to additional...

...are expressed and purified from plasmids in our repository....summarize antibody production and storage techniques, and explain common applications. View...some of our other resources to help you choose, store, or use your antibody. Antibody Blog Posts Recipient...