...the transfer plasmid, a plasmid encoding Rep and serotype-specific Cap genes, and a plasmid encoding adenoviral...Control Mycoplasma The 293T cell line was obtained from Takara, and is routinely tested for mycoplasma contamination... tested for mycoplasma contamination. To date, Addgene has never had a case of mycoplasma contamination... coli . In addition, plasmids are typically prepared using endotoxin-free plasmid purification kits. Resources...contamination is minimized by using an endotoxin-free plasmid purification protocol. To minimize the immunogenic...contamination using mycoplasma detection kits. The cell line is maintained for ~20 passages before being...off-line and discarded. Confirmation of Transfer Plasmid Addgene uses a rigorous barcode matching system...

...collection of plasmids that are available to industry. Learn more about the available plasmids and how to...to Industry Featured Collections Search Plasmids Browse Plasmids Browse Antibodies Ordering and MTA Information...see all of the plasmids that are currently available to industry scientists. ID Plasmid Gene/Insert PI...Biolabs Cancer Research from Sinai Health System Plasmids from the Structural Genomics Consortium Solute...Foundation Search Use Addgene's Search to find plasmids available to industry scientists while also adding...Toolkit Plant Expression Sylvestre Marillonnet 95 plasmids for cloning and assembling standardized parts ... ready-to-use recombinant antibodies made from plasmids in our collection! These monoclonal antibodies...

... Backbones Plasmid Description Industry mEmeralds Plasmid Description Industry Reds Plasmid Description...Industry Blues Plasmid Description Industry Cyans Plasmid Description Industry Oranges Plasmid Description...Greens Plasmid Description Industry Yellows Plasmid Description Industry Photoactivatables Plasmid Description...suggestions for other plasmids that should be added to this list? Fill out our Suggest a Plasmid form or e-mail... Plasmid Collections Fluorescent Proteins Michael Davidson...

...to view all plasmids available from the article. Click on the PI link to view all plasmids available from... all other plasmids from that article. Click on the PI link to view all available plasmids from this ...suggestions for other plasmids that should be added to this list? Fill out our Suggest a Plasmid form or e-mail...Synthetic Biology plasmids for cloning and genomic tools. Plasmid... Plasmid Collections Synthetic Biology Cloning...Browse Addgene's collection of synthetic biology plasmids related to Cloning and Genomic Tools for library...Construction Integration Recombination Shuttle Vectors Plasmids for Cloning and Genomic Tools Search the table...

...TCGATGAATTCGAGCTCG–3' ID Plasmid Selectable Marker Tags Publication FP Overexpression Plasmids for constitutive... Other Plasmids Bas Teusink Lab Materials Kurt Thorn Lab Materials Fluorescent Protein Plasmids & Resources...Find plasmids to overexpress various fluorescent markers and tag your proteins of interest with fluorescent...Materials Kurt Thorn Lab Materials Fluorescent Protein Plasmids & Resources FP Tagging FP Overexpression Resources...codon-optimized fluorescent proteins (FPs) in a variety of plasmids allows for constitutive overexpression of FPs ...proteins. These vectors are based on the pFA6a-link plasmid (Sheff et al., 2004; Lee et al., 2013), and are...codon-optimized FPs in yeast. Vectors are based on the pDRF1 plasmid (Loqué et al., 2007) containing the PMA1 promoter...

...usage of the pSpCas9(BB) plasmid (pX330) (Addgene plasmid ID 42230). This plasmid allows for the simultaneous...utilized, such as pX458 (Addgene plasmid ID 48138) or pX459 (Addgene plasmid ID 48139), which include GFP ... with a PCR cloning kit) into a plasmid vector. Transform the plasmid into DH5α E. coli cells and plate...Mammalian Cell Lines Other Addgene Resources CRISPR Plasmids CRISPR Guide Addgene Blog Posts Addgene is proud...multiple sgRNAs may be expressed from a single plasmid. Add “CACC” before the 20-mer guide sequence and... protocol involves the delivery of CRISPR/Cas9 plasmids by electroporation 4 . This protocol is described...However, transient transfection of CRISPR/Cas9 plasmids may be successfully adapted to numerous cell ...

...Generally, you will need two plasmids, as depicted in the figure below: A plasmid expressing the tRNA and ... included. Browse Synthetase Plasmids The table below highlights plasmids that contain aminoacyl tRNA ...Many of the plasmids also contain one or more copies of the cognate tRNA gene. ID Plasmid Synthetase Origin...Abhishek Chatterjee Browse Target Plasmids The table below highlights plasmids that contain genes with modified...Addgene's guide to plasmids and strains useful for expanding the genetic code in bacteria and mammalian... Plasmid Collections Genetic Code Expansion Genetic Code Expansion You may also like... GCE4All...tRNA synthetases are introduced into the cell on plasmids and the new amino acid is introduced in the media...

...human cancers. Ras Pathway Plasmids Click on a name to find available plasmids for the gene, or browse the...suggestions for other plasmids that should be added to this list? Fill out our Suggest a Plasmid form or e-mail...Addgene's collection of plasmids for the Ras pathway. Ras is a small GTPase and constitutively active...mTOR Pathway p53 Pathway Background Ras Pathway Plasmids Ras Gene List Resources Background Ras is a small...Schematic of Ras signaling cascade that starts at the plasma membrane. Cancer Impact Ras is ubiquitously expressed... - Gene List Click on a name to find available plasmids for the gene. For components with multiple subunits...v-akt murine thymoma viral oncogene homolog 3 ALK Anaplastic lymphoma receptor tyrosine kinase ARHGAP35 Rho...

...NeuroMab/Trimmer Lab Plasmid Collection R-mAb Plasmids scFv Plasmids Nanobody Plasmids Protocols References... Antibody Plasmid Collection NeuroMab Plasmid Collection...distinct IgG subclass from the conventional mAb to facilitate multiplex labeling with subclass-specific secondary... the plasmid, followed by the SV40 polyA tail (SV40 pA). Recombinant Monoclonal Antibody Plasmid Collection...following plasmids can be used to produce R-mAbs from transfected mammalian cells. Addgene ID Plasmid Target...Mouse IgG2a 114542 Anti-LRP4 (cytoplasmic) [N295B/66R] LRP4 (cytoplasmic) Mouse Mouse IgG2a 114544 Anti-GABA...Mouse IgG2a 177555 Anti-ADAM22 (cytoplasmic) [N46/30R] ADAM22 (cytoplasmic) Mouse Mouse IgG2a 177556 Anti-Rufy3...

...Fluorescent Protein Plasmids & Resources You May Also Like... Luminescence: Luciferase Plasmids Blog: Which FP...Monomer? Addgene's plasmid repository contains a variety of fluorescent protein plasmids. Use this guide ...applications of fluorescent proteins and to find the plasmids that are available from Addgene's depositing scientists... of fluorescent proteins (FPs) and to find the plasmids that are available from Addgene's depositing scientists... scientists. Browse Fluorescent Protein Plasmids by Category Empty Backbones Understand the localization...genetically encoded fluorescent biosensors. Optogenetics Plasmids | Guide Use light to detect, measure, and control...with either actuators or sensors. Chemogenetics Plasmids | Guide Use small molecules to activate genetically...

... empty backbone plasmids are ideal for single or multicolor in vivo imaging. Plasmids for In Vivo Imaging...Emission Brightness Find Plasmids mCardinal 604/659 18 All mCardinal plasmids from article mNeptune 600...suggestions for other plasmids that should be added to this list? Fill out our Suggest a Plasmid form or e-mail...Plasmids for in vivo imaging using far red, near-infrared, and photoactivatable fluorescent proteins.... Plasmid Collections Fluorescent Proteins In Vivo Imaging Guide Fluorescent Proteins: In Vivo Imaging...imaging is a powerful tool used to study individual plasmids or protein-protein interactions in deep-tissue...Near-Infrared Protein Excitation/Emission Brightness Find Plasmids iRFP670 643/670 12.7 piRFP670-N1 miRFP670 642/...

... CRISPR flyCRISPR Lab CRISPR Plasmids flyCRISPR Plasmids Available from Addgene You may also like...window) Article Individual plasmids can be ordered via the links below: ID Plasmid 46294 pBS-Hsp70-Cas9 : ...Gratz, et al. (2013). Plasmid is low copy number. 45946 pU6-BbsI-chiRNA : Plasmid for expression of chiRNA...Browse CRISPR Cas9 gRNA plasmids for drosophila research from the O'Connor-Giles, Wildonger, and Harrison...within a month. phsp70-Cas9 and a single chiRNA plasmid can be coinjected to generate mutations via imperfectly... repaired DSBs. Coinjection of phsp70-Cas9 and plasmids encoding two chiRNAs can induce defined deletions...cloned by annealed oligos into the pU6-BbsI-chiRNA plasmid via the BbsI restriction sites. Design oligos based...

...and FLASH You may also like... Keith Joung Lab plasmids Daniel Voytas Lab plasmids CRISPR plasmids This...repeat arrays assembled using the REAL, REAL-Fast or FLASH methods TALengineering...This page lists plasmid vectors into which DNA fragments encoding TAL effector repeat arrays (assembled...assembled using the REAL, REAL-Fast or FLASH methods) can be cloned for expression as TALENs. Additional details...details about the REAL, REAL-Fast, and FLASH assembly methods can be found on the TALengineering website...with the Joung Lab Individual REAL TALE Repeat Plasmids in the Joung Lab REAL Assembly TALEN Kit . Vectors...domains ( Sander et al., Nat Biotechnol. 2011 ): ID Plasmid C-terminal 0.5 TALE repeat Binds 32285 JDS70 NI...

... entity, please see our Plasmid Distribution to Industry page. These plasmids have not been expressed ...Addgene. Plasmids Use the search bar or sort buttons to find SARS-CoV-2 and common coronvirus plasmids based...Browse plasmids encoding SARS-CoV-2 viral proteins. Depositor...Ginkgo Bioworks COVID-19 Plasmid Collection (Link opens in a new window) Researchers developing novel ...Addgene has partnered with Ginkgo to make these plasmids available to scientists in academic and industrial...) Truncated for making pseudovirus (Ctrunc) ID Plasmid Description Mutations Tags Industry Additional ...Ginkgo Bioworks, Shared by Addgene: SARS-CoV-2 Plasmids for Many Expression Systems for a summary of the...

...publications associated with Addgene materials. Plasmids ID Plasmid Description PI Viral Preps Addgene distributes...research. Browse the BRAIN Initiative collection of plasmids, viral preps, and antibodies here. Collections...dissemination to the research community. Browse plasmids , viral preps , and antibodies created with support...distributes ready-to-use viral preps made from plasmids in our collection! AAV are distributed as high-quality...pAAV-CAG-FLEX-Archon1-KGC-EGFP-ER2 AAV production plasmid encoding for Archon1 fluorescent voltage reporter... pZac2.1-GfaABC1D-mCherry-hPMCA2w/b Expresses a plasma membrane calcium pump hPMCA2w/b Baljit Khakh 112000...IRES EGFP Chemogenetics Cre-dependent expression plasmid Scott Sternson 119741-AAV9 AAV SYN flex PSAM4 GlyR...

...collection of synthetic biology plasmids for use in algae. Algal Plasmids Search the table by keyword or...to view all plasmids available from the article. Click on the PI link to view all plasmids available from...suggestions for other plasmids that should be added to this list? Fill out our Suggest a Plasmid form or e-mail...Addgene's collection of synthetic biology plasmids for use in algae. Plasmid... Plasmid Collections Synthetic Biology Algal... from this depositor's lab. Plasmid Description Gene/Insert Vector Type PI Publication Do you have suggestions...

...collection of synthetic biology plasmids for use in bacteria. Bacterial Plasmids Search the table by keyword...to view all plasmids available from the article. Click on the PI link to view all plasmids available from...suggestions for other plasmids that should be added to this list? Fill out our Suggest a Plasmid form or e-mail...collection of synthetic biology plasmids for use in bacteria. Plasmid... Plasmid Collections Synthetic Biology Bacterial... from this depositor's lab. Plasmid Description Gene/Insert Vector Type PI Publication Do you have suggestions...

...collection of synthetic biology plasmids for use in fungus. Fungal Plasmids Search the table by keyword or...to view all plasmids available from the article. Click on the PI link to view all plasmids available from...suggestions for other plasmids that should be added to this list? Fill out our Suggest a Plasmid form or e-mail... collection of synthetic biology plasmids for use in fungus. Plasmid... Plasmid Collections Synthetic Biology Fungal... from this depositor's lab. Plasmid Description Gene/Insert Vector Type PI Publication Do you have suggestions...

... of synthetic biology plasmids for use in mammalian systems. Mammalian Plasmids Search the table by keyword...to view all plasmids available from the article. Click on the PI link to view all plasmids available from...suggestions for other plasmids that should be added to this list? Fill out our Suggest a Plasmid form or e-mail...collection of synthetic biology plasmids for use in mammalian systems. Plasmid... Plasmid Collections Synthetic Biology... from this depositor's lab. Plasmid Description Gene/Insert Vector Type PI Publication Do you have suggestions...

...collection of synthetic biology plasmids for use in plants. Plant Plasmids Search the table by keyword or...to view all plasmids available from the article. Click on the PI link to view all plasmids available from...suggestions for other plasmids that should be added to this list? Fill out our Suggest a Plasmid form or e-mail... collection of synthetic biology plasmids for use in plants. Plasmid... Plasmid Collections Synthetic Biology Plant... from this depositor's lab. Plasmid Description Gene/Insert Vector Type PI Publication Do you have suggestions...