...Genomics Consortium Plasmids All SGC Plasmids Plasmid Gene/Insert Vector Type Backbone Mutations Tags Industry...

...Structural Genomics Consortium Plasmids - Toronto Plasmid Gene/Insert Vector Type Backbone Mutations Tags Industry...

...markers of general cellular organelles and structures. ID Plasmid Structure Targeting Gene/Signal Fluorescent...Plasmids encoding fluorescent proteins tagged with genes or peptides with known subcellular localization ...cytoskeletal element. ID Plasmid Structure Targeting Gene/Signal Fluorescent Protein PI 26722 GFP-cortactin...cellular junctions. ID Plasmid Structure Targeting Gene/Signal Fluorescent Protein PI 71367 Human Beta-catenin...pathway components. ID Plasmid Structure Targeting Gene/Signal Fluorescent Protein PI 110060 FUW mCherry-GFP-LC3...Collection FRET Biosensors Optogenetics Fluorescent Protein Blog Posts Background General Cytoskeletal Junctions...of your protein of interest with known markers. Addgene has assembled a collection of fluorescent fusion...

...available from this depositor's lab. Plasmid Description Gene/Insert Vector Type PI Publication Do you have suggestions...Browse Addgene's collection of synthetic biology plasmids for use in algae. Plasmid...Standards SynBio Blog Genome Engineering Browse Addgene's collection of synthetic biology plasmids for use...out our Suggest a Plasmid form or e-mail help@addgene.org to help us improve this resource!...

...available from this depositor's lab. Plasmid Description Gene/Insert Vector Type PI Publication Do you have suggestions...Browse Addgene's collection of synthetic biology plasmids for use in bacteria. Plasmid...Standards SynBio Blog Genome Engineering Browse Addgene's collection of synthetic biology plasmids for use...out our Suggest a Plasmid form or e-mail help@addgene.org to help us improve this resource!...

...available from this depositor's lab. Plasmid Description Gene/Insert Vector Type PI Publication...A list of Addgene plasmids for use in synthetic biology applications, or from synthetic biology labs....to see added to this list, please email help@addgene.org . Synthetic Biology Plasmids Search the table...

...available from this depositor's lab. Plasmid Description Gene/Insert Vector Type PI Publication Do you have suggestions...Browse Addgene's collection of synthetic biology plasmids for use in fungus. Plasmid...Standards SynBio Blog Genome Engineering Browse Addgene's collection of synthetic biology plasmids for use...out our Suggest a Plasmid form or e-mail help@addgene.org to help us improve this resource!...

...available from this depositor's lab. Plasmid Description Gene/Insert Vector Type PI Publication Do you have suggestions...Browse Addgene's collection of synthetic biology plasmids for use in plants. Plasmid...Standards SynBio Blog Genome Engineering Browse Addgene's collection of synthetic biology plasmids for use...out our Suggest a Plasmid form or e-mail help@addgene.org to help us improve this resource!...

...available from this depositor's lab. Plasmid Description Gene/Insert Vector Type PI Publication Do you have suggestions...Browse Addgene's collection of synthetic biology plasmids for use in yeast. Plasmid...Standards SynBio Blog Genome Engineering Browse Addgene's collection of synthetic biology plasmids for use...out our Suggest a Plasmid form or e-mail help@addgene.org to help us improve this resource!...

...available from this depositor's lab. Plasmid Description Gene/Insert Vector Type PI Publication Do you have suggestions...Browse Addgene's collection of synthetic biology plasmids for use in mammalian systems. Plasmid...Standards SynBio Blog Genome Engineering Browse Addgene's collection of synthetic biology plasmids for use...out our Suggest a Plasmid form or e-mail help@addgene.org to help us improve this resource!...

...available from this depositor's lab. Plasmid Description Gene/Insert Vector Type PI Publication Do you have suggestions...Browse Addgene's collection of synthetic biology plasmids for use in C. elegans. Plasmid...Standards SynBio Blog Genome Engineering Browse Addgene's collection of synthetic biology plasmids for use...out our Suggest a Plasmid form or e-mail help@addgene.org to help us improve this resource!...

...Adeno-associated virus (AAV) vectors are safe and useful gene delivery tools that effectively transduce many mammalian... more information on its performance. ID Plasmid Gene/Insert Promoter PI Additional Resources Viral Vector...recommended for research-grade use. Many chemogenetic, optogenetic, recombinase, and biosensor transfer plasmids...experiments as soon as they receive their AAV prep from Addgene — eliminating the steps to produce, purify, and...this service. Select any eligible plasmid in the Addgene repository in your choice of: serotype (AAV1, AAV2...Large 50 × 100 µL aliquots 5 mL *AAV2 yields are generally lower, with a minimum titer of 2 × 10 12 GC/mL...production Density centrifugation purification Addgene’s comprehensive quality control process: Titering...

...California, San Francisco Browse Plasmids ID Plasmid Gene/Insert Vector Type Backbone Mutations Tags PI...between the Cell Migration Consortium (CMC) and Addgene, which highlights CMC depositing labs and plasmids...collaboration between the Cell Migration Consortium and Addgene. Member laboratories of the Cell Migration Consortium...plasmids useful for cell migration research at Addgene. CMC investigators who have deposited plasmids ...

...plasmids that are currently available. ID Plasmid Gene/Insert Vector Type Backbone Mutations Tags PI...of drug targets in Alzheimer’s disease (AD) by generating validated resources for emerging targets in AD...IUSM-Purdue TREAT-AD centers will be deposited at Addgene and available to request. Plasmids Browse the table...

...scaffold to allow scientists to selectively modulate gene expression....Backbones FRET Subcellular localization Biosensors Optogenetics Background Fluorescent proteins such as GFP ...out our Suggest a Plasmid form or e-mail help@addgene.org to help us improve this resource!...

...tags for you to create fusion proteins with your gene of interest. Please visit Michael Davidson's Molecular...Empty Backbones FRET Subcellular localization Optogenetics The lab of Michael Davidson from Florida State...out our Suggest a Plasmid form or e-mail help@addgene.org to help us improve this resource!...

...PubMed 20525357 Plasmid ID Plasmid Name Zebrafish Gene 28214 SI1-Unc5b Unc5b 28215 SI2-Unc5b Unc5b 28216...

...editing in mammalian systems. Mammalian ID Plasmid Gene/Insert PI Publication Do you have suggestions for...Transcriptional Regulation Activate Interfere Epigenetics RNA Targeting RNA Targeting RNA Editing Other...out our Suggest a Plasmid form or e-mail help@addgene.org to help us improve this resource!...

...example, choosing target sites that span the knock-in gene. For making mutations, one good way is to mutate...be okay if you are introducing a selection marker gene. The homology arms should be no more than 100bp ...for my genomic PCR, but couldn't amplify the EMX1 gene using same primer you used in the Science paper ...You may also like... Zhang Lab CRISPR plasmids General CRISPR Plasmids Dr. Le Cong, the first author of...additional PAM sequences should not be sufficient to generate off-target DSBs; there needs to be extensive homology...below are considerations for designing HR donor. Generally if off-target (non-specific) cleavage is not a...purified oligo is fine based on new data). For general transfection, you could use 1ul of 10uM oligo stock...

...SARS-CoV-2 and common coronvirus plasmids based on: Gene insert (e.g. spike, envelope, nsp3, OC43, NL63, ...Additional Resources Read Addgene's Blog Synthesized by Ginkgo Bioworks, Shared by Addgene: SARS-CoV-2 Plasmids...for a summary of the Ginkgo collection at Addgene. Addgene's COVID-19 and Coronavirus Plasmids & Resources... viral proteins in several expression systems. Addgene has partnered with Ginkgo to make these plasmids...expressed or functionally validated by Ginkgo or Addgene. Plasmids Use the search bar or sort buttons to...to COVID-19 that may be of use to scientists. Addgene's Protocol for RNA Extraction Without A Kit...