...that was created for systematic screening of extracellular protein interactions to identify novel receptor-ligand...22080952 Plasmid ID Plasmid Name 73089 bio-CD4d3+4-Kell blood group 73090 bio-CD4d3+4-ADL1 73091 bio-CD4d3...

... tools that effectively transduce many mammalian cell types. AAV is used in a wide range of experiments...Tetracycline Transactivators, Affinity Purification, Cell Ablation Monosynaptic Neuronal Tracing Rabies Virus-Based...

...sequence. DSBs are preferentially repaired in the cell by non-homologous end joining (NHEJ), a mechanism...PAM site. When a repair template is present, the cell may repair a DSB using homology-directed repair ...

...Fluorescent Protein Resources: Empty Backbones FRET Subcellular localization Optogenetics Background In vivo ...stable imaging of protein interactions in living cells and live mice. Proteins for in vivo imaging have...

...allowing a scientist to control signaling within cells expressing these engineered receptors. Chemogenetic...engineered receptor is coupled to and the specific cell type where the receptor is expressed, these receptors...

...Can I directly transfect cloning grade DNA into cells? Cloning grade DNA is designed for use in cloning...cloning applications. Direct transfection into cells is not recommended. Can I order more than 2 µg of cloning...

...then be used to infect permissive cells that express ACE2, the cellular receptor for SARS-CoV-2, in a single...

... inosine, which is treated like guanosine by the cell, creating an A to G (or T to C) change. Adenine ...hypermutation) in a wider editing window, and are thus well suited to directed evolution applications. Examples...

...application-specific validation and quality control by Addgene as well as by our trusted partner labs. Learn More ID Recombinant...governs the transfer of biological materials and intellectual property rights, and provides liability protection...

...activation activity of three-fold or greater in a well-characterized bacterial two-hybrid reporter system...efficient gene modification. Maeder ML et al. Mol Cell. . 2008. Jul 25. 31(2):294-301. PubMed PMID 18657511...

... PM-8:00 PM Group Dinner Relax and Connect with Fellow Speakers and Attendees Friday, September 21 9:00...GCaMP6f-P2A-nls-dTomato Detailed protocol: AAV Production in HEK293 Cells Please share materials by uploading to your Dropbox...

... tools that effectively transduce many mammalian cell types. AAV is used in a wide range of experiments...distributed preps around the globe and shared our well-developed and trusted protocols and peer-reviewed...

...that was created for systematic screening of extracellular protein interactions to identify novel receptor-ligand...Aggregation Receptor 1 (PEAR1) Ligand. Sun Y et al., Mol Cell Proteomics. 2015 May; 14(5):1265-74. PubMed 25713122...

...therapies for brain disorders by enabling region- and cell-specific gene-delivery in the mouse brain through...Black SF, Bonaguro RJ, Brauer E, Candido TR, Castellarin M, Chen J, Chen Y, Cheng JC, Chopra V, Docking...

...Cas protein and a target-specific gRNA in the same cell at the same time. Single plasmids containing both...Alternatively, gRNA-only plasmids can be used in cells or organisms in which Cas9 has been integrated into...

...emerging targets in AD and creating high-quality, well-characterized lead molecules for prioritized targets...

...application-specific validation and quality control by Addgene as well as by our trusted partner labs. To learn more about...

...that was created for systematic screening of extracellular protein interactions to identify novel parasite-parasite...

...listed below have the CMV promoter for mammalian cell expression and a T7 promoter for in vitro transcription...

... time and are potentially heritable in dividing cells. CRISPR Activate and Interfere technologies also...