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Showing: 81 - 100 of 120 results

  1. Zinc Finger Consortium: OPEN Reagents

    Type
    Collection
    ...Information Protocols & Resources Contents of Kit Original Publication Oligomerized pool engineering (OPEN...tools for using the bacterial two-hybrid system originally described by Hochschild and colleagues ( Dove...

  2. Malate Dehydrogenase CUREs Community Collection

    Type
    Collection
    ...institutions. With these plasmids, faculty can begin a sustainable MDH-related CURE focusing on protein expression...experiences (CURE) impacts student learning and attitudinal outcomes: A study of the Malate dehydrogenase...

  3. Adenovirus Plasmids

    Type
    Collection
    ...the most common of which involves homologous recombination of adenovirus plasmids in either mammalian cells...Strain Description PI 16398 BJ5183 Strain for recombination between shuttle plasmid and adenoviral plasmid...

  4. CRISPR Plasmids - Yeast

    Type
    Collection
    ... Catalytically dead dCas9 fused to a cytidine deaminase protein becomes a specific cytosine base editor...Selectable Marker PI Publication Purify A catalytically inactive Cas9 (dCas9) can be used to purify a region of...

  5. Microbiology Resources

    Type
    Collection
    ...and Dutzler Labs EasyClone 2.0 Yeast Toolkit - Borodina Lab Golden GATEway cloning system - Wittbrodt ...MoClo : Modular cloning for applications like recombinant protein purification and cell-free circuit design...

  6. Worm Expression Resources

    Type
    Collection
    .... elegans by combining expression of the FLP recombinase from either a heat-shock promoter or a tissue-specific...WormBook - A comprehensive, open-access collection of original, peer-reviewed chapters covering topics related...

  7. CRISPR Plasmids - Bacteria

    Type
    Collection
    ... Catalytically dead dCas9 fused to a cytidine deaminase protein becomes a specific cytosine base editor...Promoter PI Publication Purify A catalytically inactive Cas9 (dCas9) can be used to purify a region of...

  8. EXPRESs plasmids for human platelets

    Type
    Collection
    ... collection plasmids for expressing secreted recombinant proteins from human platelets that was created...52180 IGFALS-bio-His 52181 ITIH4-bio-His 52182 SERPINA-bio-His 52183 SMR3B-bio-His 52184 TF-bio-His 52185...

  9. TALEN Guide

    Type
    Collection
    ...TALEN Kits CRISPR Plasmids Zinc Finger Consortium Originally published in Addgene's December 2011 Newsletter...Bogdanove Golden Gate TALEN kit. In this example, the final array is composed of 18 TAL effector repeats. The...

  10. Lentivirus Plasmids

    Type
    Collection
    ...Cheng 17452 pLenti CMV Puro DEST 3rd Gateway destination plasmid with constitutive or inducible expression...Fraser 15948 pLOVE 3rd Tet inducible gateway destination plasmid for cDNA expression Ramalho-Santos 24129...

  11. Tetracycline Inducible Expression

    Type
    Collection
    ...developed. tTA was created by fusing tetR with the C-terminal domain of VP16 (virion protein 16), an essential...appropriate. If your gene of interest should be mostly inactive, and only turned on occasionally, using tetracycline...

  12. Penn Vector Core Partnership with Addgene

    Type
    Collection
    ...Chemogenetics (including DREADDs) Optogenetics Recombinases Calcium Sensors and other Biosensors Controls...pENN.AAV.CB7.CI.mCerulean.WPRE.RBG James M. Wilson Cre Recombinase AV-1-PV1090 105537-AAV1 pENN.AAV.CMVs.Pl.Cre....

  13. Validated gRNA Sequences

    Type
    Collection
    ... 42242 cut S. pyogenes 23360964 Joung Ebf C. intestinalis GCTGAGGGTTGGACAACAGG 59990 cut S. pyogenes 25336740...pyogenes 26178787 Winslow negative control C. intestinalis GCTTTGCTACGATCTACATT 60006 cut S. pyogenes 25336740...

  16. CRISPR Plasmids - dCas9-FokI

    Type
    Collection
    ...Design Tools CRISPR Blog Posts A catalytically inactive Cas9 (dCas9) is fused to FokI nuclease. When FokI...

  19. KLF Research Plasmids

    Type
    Collection
    ...feature of the KLF family is the presence of three C-terminal Krüppel-like zinc fingers which bind to CACCC ...

  20. CRISPR Plasmids - RNA Editing

    Type
    Collection
    ...the catalytic domain of ADAR2(E488Q) adenosine deaminase to catalytically dead Cas13b creates a programmable...
Showing: 81 - 100 of 120 results