...added to this list? Fill out our Suggest a Plasmid form or e-mail [email protected] to help us improve this...

...fill out our form to let us know what you would like to see available in viral prep format. Please note...

...added to this list? Fill out our Suggest a Plasmid form or e-mail [email protected] to help us improve this...researcher safety. Read our Lentiviral Guide for more information about: Lentiviral components 2nd versus 3rd generation...transgene or shRNA. See article for detailed information about cloning & additional plasmids. Aebischer...

...added to this list? Fill out our Suggest a Plasmid form or e-mail [email protected] to help us improve this...expression for Gateway cloning; selection cassette in format: PGK-rtTA-2A-puro; see article for tagged insert...transgene or shRNA; see article for detailed information about cloning and additional plasmids tetR-KRAB...expression of a miR-shRNA; selection cassette in format: rtTA3+IRES+Neo; see article for additional selection...

...added to this list? Fill out our Suggest a Plasmid form or e-mail [email protected] to help us improve this...described below. Read our CRISPR 101 post for more information about HDR/repair template design. How to use ...

...added to this list? Fill out our Suggest a Plasmid form or e-mail [email protected] to help us improve this...myristoylation signal For more information about tags, including amino acid sequences, see...delivery. Visit our viral vector page for more information. Below are some common delivery methods: Delivery... host genome. View our AAV page for more information. pAdTrack-CMV - Shuttle vector...

...Protein phosphorylation is one of the most abundant forms of posttranslational modifications in cells and ... is anticipated to be useful for laboratories performing phosphoproteomic experiments in need of reference...

...protein and in vitro transcribed gRNA are combined to form a Cas9-gRNA complex, which is delivered to cells... be used to examine off-target effects. More information on each of these techniques can be found in our...

...fill out our form to let us know what you would like to see available in viral prep format. Please note...Plasmids and Optogenetics Guide page for more information about this field. Use the table and filters below...

...fill out our form to let us know what you would like to see available in viral prep format. Please note... vivo testing of these viral preparations was performed by the Karpova lab. Results for our quality control...

...added to this list? Fill out our Suggest a Plasmid form or e-mail [email protected] to help us improve this... AAV production. Read our AAV Guide for more information about: AAV components Common uses of AAV Viral...plasmids encode the AAV genome (i.e., the genetic information that will be contained in the virion) and/or ...

...fill out our form to let us know what you would like to see available in viral prep format. Please note...Addgene's blog on Systemic Capsids . To review performance data of each of these serotypes, please refer...window) ) to cross the blood brain barrier. For information on whether the PHP.eB serotype will work in your...pAAV-CAG-GFP CAG GFP Control Boyden Citation Information When using the Caltech serotypes in future publications...

...ll express your gRNA and Cas9 protein, which will form a ribonucleoprotein complex through interactions...effector, composed of several separate Cas enzymes that form a complex called Cascade (see Cas3 for more details...with Cas3 Rather than initiating DNA cleavage to form a break, Cas3 initiates single-strand DNA degradation...dCas9 Nuclease dead Cas9, an enzymatically inactive form of Cas9; can bind, but cannot cleave DNA DSB D ouble...Cas9 undergoes a second conformational change upon target binding. In this formation, its nuclease domains...detection platform. Based on this non-specific cleavage, a Cas13a-based molecular detection platform, termed...positively-charged grooves on Cas9. gRNA binding induces a conformational change in Cas9, shifting it into an active,...

...added to this list? Fill out our Suggest a Plasmid form or e-mail [email protected] to help us improve this...enterobacterial genomes. The bacteria of interest are transformed with the appropriate construct, grown at the ...inside cells just isn’t enough to get all the information you need. If you want to dissect an individual...to the periplasm which may aid disulfide bond formation or prevent toxicity. Compatible with Gateway cloning...for protein localization to aid disulfide bond formation F Xavier Gomis-Ruth Kit containing plasmids with...Reporter system for testing hetero- and homo-dimer formation. Proteins of interest are fused to AraC or a dominant... from the araBAD promoter whereas heterodimer formation with dominant negative AraC decreases GFP expression...

...added to this list? Fill out our Suggest a Plasmid form or e-mail [email protected] to help us improve this...maximum of NanoLuc to that of the fluorophore. Conformationally destabilized Firefly luciferase mutants : ...white screening can be used for screening of transformants in cloning process. Lynne Postovit 119760 pSGDlucV3.0...

...individual crRNAs. (3) The tracrRNA and Cas9 nuclease form a complex with each individual, unique crRNA. (4...multiple plasmids used for various platform applications. For more information on CRISPR, check out Addgene’...work to develop a highly specific, programmable platform well-suited for various biological and translational...

...function. This is due to the high frequency of indels formed on non-deleted alleles 2 , which are likely to ... CRISPR References & Information Genomic Deletions in Mammalian Cell Lines CRISPR...ligation to occur in one reaction (see step 3.2 ). Transform 10 μl of DH5α E. coli cells with 1 μl of reaction...colonies and inoculate into a mini-prep culture. Perform mini-prep for each sample and sequence each colony...may be scaled based on the required DNA yield. Perform maxi-prep for each CRISPR/Cas9 construct. Transfecting... cell type used at limiting dilution prior to performing this step to reliably obtain approximately 30...genomic DNA isolation may be utilized to be able to perform PCR in step 6.3 . Assemble a 20 μl PCR with the...

...activate all known coding isoforms form the RefSeq database (23,430 isoforms) for gain-of-function screening...mouse) for performing genome-wide knockout screens. The libraries are available in two formats: 1 vector...et al ., Nature Protocols 2013). For cloning information, please view: Zhang Lab General Cloning Protocol...the Addgene GeCKO Library CRISPR page for more information. Full references are below. Return to top Cas9...screening. For more information, please visit the Addgene SAM library page . Targeted gene activation using...recombinase-2A-EGFP-KASH Detailed backbone cloning information: CRISPR-Cas9 mouse toolbox protocol 637.8 KB ...

...added to this list? Fill out our Suggest a Plasmid form or e-mail [email protected] to help us improve this...Plasmids . For more information on stem cells, visit the NIH Stem Cell information page. Reprogramming...and then select the article link to find more information. Delivery Method Species Description Article ...and then select the article link to find more information. Convert From Convert To Delivery Method Species...

...fill out our form to let us know what you would like to see available in viral prep format. Please note...