...Version 3 90294 (1 plasmid) 125517 (2 plasmid) Knockout Human Moffat 3rd 4 70,948 Toxoplasma Knockout 80636...Broad GPP genome-wide Brunello 73179 (1 plasmid) 73178 (2 plasmid) Knockout Human Doench and Root 3rd 4 ...76,441 Broad GPP genome-wide Brie 73632 (1 plasmid) 73633 (2 plasmid) Knockout Mouse Doench and Root 3rd 4 ...GPP kinome Brunello 75314, 75315 (1 plasmid) 75312, 75313 (2 plasmid) Knockout Human Doench and Root 3rd... 3,052 Broad GPP kinome Brie 75317 (1 plasmid) 75316 (2 plasmid) Knockout Mouse Doench and Root 3rd 4 ...20,051 Human GeCKO v2 1000000048 (1 plasmid) 1000000049 (2 plasmid) Knockout Human Zhang 3rd 6 123,411...lentiCRISPR v2-Blast 1000000173 Knockout Mouse Liu 3rd 10 185,836 Mouse GeCKO v2 1000000052 (1 plasmid) 1000000053...

...Rudolf Jaenisch labs Plasmids MECP2 Plasmids The table below highlights plasmids that express or target... generating iPSCs using plasmids, please see Addgene's Plasmids for Stem Cell Research page. CRISPR Tools...Broswe plasmids and other research tools for use in Rett Syndrome research. Plasmid... Plasmid Collections Rett ...Rett Syndrome Rett Syndrome Plasmid Collection and Resource Center MECP2 Animal Models Human Cell Lines CRISPR... CRISPR Tools Plasmids Resources The (Link opens in a new window) Rett Syndrome Research Trust (RSRT) ...individual laboratories to assemble a Rett Syndrome plasmid resource for the scientific research community....

...Control of Gene Expression. Qi LS, Larson MH, Gilbert LA, Doudna JA, Weissman JS, Arkin AP, Lim WA. Cell ....Regulation of Transcription in Eukaryotes. Gilbert LA, Larson MH, Morsut L, Liu Z, Brar GA, Torres SE, ... CRISPR Qi Lab CRISPR Plasmids Qi Labs CRISPR Plasmids Available from... genome. 1. Two-plasmid CRISPRi system for bacterial gene knockdown The first plasmid ( pdCas9_bacteria... both plasmids in bacteria could cause up to 300-fold repress on targeted genes. 2. Two-plasmid CRISPRi... 23849981 . Individual plasmids can be ordered via the links below: ID Plasmid Description 44246 pdCas9...fusion expression plasmid 44248 pgRNA-humanized A customizable gRNA expression plasmid for use with either...

...Species) Organism/Expression System Plasmids Mammalian Golenbock Lab Plasmids - Includes tagging with CFP, YFP...Lentiviral Plasmids - Fluorescently tag your gene for lentiviral expression Verkhusha Lab Plasmids - Enhanced...Mammalian Plasmids - Includes tagging with mCherry, mCitrine, mCerulean Davidson Lab Plasmids - Includes...suggestions for other plasmids that should be added to this list? Fill out our Suggest a Plasmid form or e-mail...Addgene has assembled a collection of empty plasmid backbones with different fluorescent tags for you...colors. Addgene has assembled a collection of empty plasmid backbones with different fluorescent tags for you...Emission (nm) Brightness pKa Maturation Structure Plasmids Sirius 355 424 4 3.0 Prone to dimerization Sirius-N1...

...reporter) pX458 plasmid family. They have made several modifications to the the pX458 plasmid to expand its...fusion shuttle plasmid for shuttling U6-guide cassettes to make dual guide expressing plasmids dCas9-KRAB-...activation CRISPR/Cas9 Plasmid Variants Figure 1: Schematic of the CRISPR Collection. Plasmids 159654 and 159655...constructs. Plasmids 171098 and 175570 allow conversion to a dual guide construct. Plasmids 175573 and ...expressed from the same plasmid by utilizing one of the dual guide shuttle plasmids to insert a second guide... #110821 ) VPR ( Addgene #63798) Plasmids from Collection ID Plasmid Description PI Publications Kaufman...Find CRISPR plasmids to study the regulatory networks involved in retinal development. Depositor...

...Grade DNA (cgDNA) for over 200 plasmids in our collection. The plasmids available in this format include...variety of popular plasmids and backbones with high cloning potential. By making these plasmids available as...individual plasmid page to find current pricing information Standard Quality Control Plasmid verified by...next generation sequencing Plasmids Available as Cloning Grade DNA ID Plasmid Description Industry PI FAQ...grade DNA preps of this plasmid available, but helps to inform us of which plasmids would be most helpful... our most popular plasmids! DNA... DNA Service: Cloning Grade DNA Service Details Plasmids Available FAQ Addgene...

...Fragmented T7 RNAP Plasmids Repressor Plasmids Sigma (σ) Factor Plasmids Terminator Plasmids Viral Vectors ... Plasmid Collections Depositor Plasmid Collections Depositor Collections...Addgene's repository contains 156,515 plasmids, which includes plasmid collections from depositors. To help... you find it helpful! You May Also Like: Plasmid Kits Plasmid Special Collections Pooled Libraries Antibodies...Collection Rett Syndrome Plasmid Collection and Resource Center TREAT-AD Plasmid Collection Visualization...Institute for Cell Science Plasmid Collection New England BioLabs Cell-Imaging Plasmid Collection Botman-Teusink... biology, and viral vector plasmids. Plasmid...

... and plasmid collections that may help with your neurodegeneration research. Disease Info Plasmid Collection...Research Tools Neurodegeneration Plasmid Collection A collection of plasmids expressing wild type or mutant...Differentiation Factors Find plasmids used to transform iPSCs and fibroblasts into neurons and more. New...Subcellular Localization, Optogenetics Plasmids, and Chemogenetics Plasmids. New and Noteworthy: Explore NeuroPAL...more. Plasmids provided by the foundation can be found on our Michael J. Fox Foundation Plasmid Resource...Find out about the plasmid collections available at Addgene that may be useful for neurodegeneration ...decision-making and language abilities. The disease can be classified as early-onset, where symptoms appear between...

... Plasmid Collections Cre-lox Plasmids Cre-lox Plasmids Additional...Cre-lox Plasmids Addgene's Blog: Cre-lox Addgene's Blog: FLEx Vectors Cre-containing Plasmids loxP Constructs...viral preparations of plasmids that are currently in our repository. ID Plasmid Description Promoter Expression...loxP plasmids, reporter genes indicate which cells have undergone Cre recombination. These plasmids can...suggestions for other plasmids that should be added to this list? Fill out our Suggest a Plasmid form or e-mail... Cre-lox plasmids for your experiments. Plasmid...alternatives from Gerald Rubin's lab. Cre-containing Plasmids Scientists have developed ways to tightly control...

...Accessory Plasmids Envelope and Packaging Plasmids Popular Lentiviral Envelope and Packaging Plasmids Popular...Envelope and Packaging Plasmids Reporter Plasmids A few examples of reporter plasmids that can be used for...particles See Addgene's Viral Plasmids and Resources page for more information. Plasmids Expressing Spike and ...Addgene plasmid #141347) is available in standard format and as a Lentiviral prep . ID Plasmid Description...COVID-19 and Coronavirus Plasmids & Resources Ginkgo Bioworks COVID-19 Plasmid Collection Return to top...suggestions for other plasmids that should be added to this list? Fill out our Suggest a Plasmid form or e-mail...Addgene's collection of plasmids for viral pseudotyping with SARS-CoV-2 spike protein. Collections...

... may also like... Zhang Lab CRISPR plasmids General CRISPR Plasmids Dr. Le Cong, the first author of one...easiest way is to co-transfect several plasmid together, with each plasmid having a targeting spacer cloned ... Genome Engineering CRISPR Zhang Lab CRISPR Plasmids Zhang Lab CRISPR FAQs Zhang Lab's CRISPR Frequently...with expression, though it is possible for the plasmid to still express without the G. Because the G is...experiments for multiplexed targeting with Zhang lab plasmids? For multiplexing CRISPR to target multiple genome...into PX330, then co-transfect or delivery the two plasmids together into your cells. If you want to use double...very big concern, you could go ahead with PX330 plasmid, clone in your target guides, test run them and...

...Collections Richard Kahn Lab Plasmids GTPase Plasmids GAP and GEF Plasmids The ARF (ADP-ribosylation factor...examples of plasmid types available for each gene. Click the gene name for a full list of plasmids available...suggestions for other plasmids that should be added to this list? Fill out our Suggest a Plasmid form or e-mail...ARF family plasmids. ARF family members include regulatory GTPases, GEFs, and GAPs. Plasmid... Plasmid Collections...Collections ARF Superfamily Plasmids for ARF Superfamily Research You may also like... Ras Pathway RAS Clone Collections...

...., Almeida, R., Gilbert, L. A., Whitehead, E. H., La Russa, M., Tsai, J. C., Weissman, J. S., Dueber, ...different classification of nucleotide base (purine-to-pyrimidine and vice versa) The first classes of base...Addgene's guide to using CRISPR plasmids in your lab, from knockouts to pooled library screening. Science... cell must be validated experimentally. Browse Plasmids: Double-Strand Break (Cut) Multiplex Genome Engineering...cases, delivering multiple gRNAs using a single plasmid ensures that all gRNAs will be expressed in the...likelihood that any cell containing the CRISPR plasmid will have all desired genomic edits carried out...genetic loci by cloning multiple gRNAs into a single plasmid — though some have achieved targeting in the double...

...BioBricks Foundation Plasmids Drew Endy Lab Plasmids Jennifer Molloy Lab Plasmids Golden Gate cloning ...Golden Gate assembly. Plasmids Explore the collection and order individual plasmids via the links below:...pOpen-UDG UDG Uracil DNA glycosylase 165541 pOpen-Fpg Fpg Formamidopyrimidine DNA glycosylase 165550 pOpen-T4 ...Browse the Open Enzyme collection of plasmids at Addgene for generating enzymes for molecular biology... (Link opens in a new window) . How to Use The plasmids in the Open Enzyme collection contain genetic ...are recommended). The selection marker for all plasmids in this collection is ampicillin. Find more information...Polymerases Other Enzymes DNA Ligases Addgene ID Plasmid Gene/insert 165503 pOpen-HSlig4 DNA Ligase IV 165514...

... like... Keith Joung Lab plasmids Daniel Voytas Lab plasmids CRISPR plasmids The Joung Lab has developed...construction of TALEN expression plasmids using REAL, REAL-Fast or FLASH assembly. This new version of ZiFiT...REAL-Fast assembly process or lists of plasmid ID numbers required for FLASH assembly. Links to additional pages...Individual REAL TALE Repeat Plasmids TALEN Expression Vectors for REAL, REAL-Fast and FLASH TALE Transcriptional...Gene Additional Plasmids compatible with the REAL Assembly TALEN Kit: TALE-LSD1 plasmids for epigenome ...TALENs, and assembly methods for REAL, REAL-Fast and FLASH Genome...method (Reyon et al., manuscript submitted), and the FLASH (Fast Ligation-based Automatable Solid-phase High-throughput...

...Collections INTRSECT Plasmid Collection INTRSECT Plasmid Collection INTRSECT Implementation Plasmids References ...novel INTSRECT plasmids for groups interested in producing their own INTRSECT plasmids. Online spreadsheet...Find intersectional expression plasmids that combine multiplexed recombinases, including Cre, Flp, and...Flp-recombinase-expressing transgenic mouse and rat lines. Plasmids In addition to EYFP and ChR2-EYFP, a large number...rabies targeting genes. Recombinases Addgene ID Plasmid Logic Sites and Mutations 55636 pAAV-EF1a-Cre None...pAAV-CaMKIIa-Flpo None Single recombinase-dependent Addgene ID Plasmid Logic Sites and Mutations 55641 pAAV-Ef1a-fDIO ...recombinase-dependent: Fluorophores Addgene ID Plasmid Logic Sites and Mutations 55650 pAAV-hSyn Con/Fon...

...little variation. Recombinant plasmids - Antibodies can be produced from plasmids, with an antibody’s genetic... genes from specific hybridoma cells into plasmids. Plasmids are then transfected into cells and induced...immune cells, or through the use of recombinant plasmids. Animals - Antibodies produced in animals make...genetic code inserted into the plasmid. This produces the least amount of variation between antibodies, allows...Isotypes The mammalian immune system produces five classes of antibodies, known as isotypes. An isotype is...antibodies can be isotype-specific or even isotype subclass-specific. This specificity can be used to ensure..., while others exist as monomers. Each antibody class therefore has a valency, or number of binding sites...

...process . Browse SynBio Plasmids by Category Cloning and Genomic Tools Browse plasmids related to cloning ...bacterial strains, including plasmid-strain functional pairs. Browse Plasmids by Model Organism Bacterial...suggestions for other plasmids that should be added to this list? Fill out our Suggest a Plasmid form or e-mail... Plasmid Collections Synthetic Biology Synthetic Biology...Gabor Balazsi Matthew Bennett Carolyn Bertozzi Nicolas Buchler George Church Cynthia Collins James Collins...Addgene can help you to share your synthetic biology plasmids with the community. To deposit, view our SynBio...reporter, and tagging vectors. Metabolism Browse plasmids related to metabolic pathways and auxiliary components...

...transfer plasmids in our repository are eligible for this service. Select any eligible plasmid in the Addgene...applicable transfer plasmid. We will help you gain legal usage of the transfer plasmid from the providing...real-world troubleshooting. Eligible Plasmids The following plasmids are eligible for this service. Submit...paper of your chosen AAV plasmid for more information on its performance. ID Plasmid Gene/Insert Promoter ...Overview Details Ordering Share Your Results Eligible Plasmids Additional Resources Adeno-associated virus (AAV...lets scientists choose from over 3,000 transfer plasmids to be packaged in their choice of five serotypes...2.5 mL, or 5 mL). Currently, we can only package plasmids in our collection in the serotypes listed here...

...are made from plasmids that are currently in our repository. You can obtain the plasmid from us or receive... list of gRNA plasmids targeting specific genes. For more information on CRISPR plasmids and function,...Resistance Activity PI 52962 lentiCas9-Blast Sp Cas9 Cut Blasticidin Expresses human codon-optimized S. pyogenes...2A GFP Zhang 61425 lenti dCAS-VP64_Blast dCAS9 (D10A, N863A) Blasticidin 3rd generation lenti vector encoding...encoding dCAS9-VP64 with 2A Blast resistance marker (EF1a-NLS-dCas9(N863)-VP64-2A-Blast-WPRE) Zhang 61426 lenti...processes. For more information, see our Guide to Plasmid Pooled Libraries . ID Name Description Genes/Insert...function, see our CRISPR plasmids resource page . Cas9 for Editing Genomic Sequences To perform genome edits,...