...and correspond to edited and non-edited cell populations. For more information, see the bioRxiv preprint...rice (Oryza sativa), tomato, corn, monkey (macaca mulatta). Cas-Designer searches for targets that maximize...design tool for genome editing, as well as gene regulation (repression and activation). Genome support for...Brachypodium distachyon, Oryza sativa, Oryzias latipes CasFinder: Flexible algorithm for identifying specific...

...effective treatments for carcinoid, pancreatic, and related neuroendocrine cancers. The NETRF believes that...Shivdasani Dana-Farber Cancer Institute Epigenetic Regulators of Intestinal Endocrine Cells and Carcinoid Tumors...Tumors Qiao Zhou Harvard University Epigenetic Regulators of Intestinal Endocrine Cells and Carcinoid Tumors...

...CRISPR plasmids for activating or upregulating gene expression in various model organisms....Cut Base Edit Nick Prime Edit Transcriptional Regulation Activate Interfere Epigenetics RNA Targeting ... to direct the dCas9-activator to promoter or regulatory regions of your gene of interest. If the plasmid...

...normal cell signaling and physiology, and, when dysregulated, can enable cancer cell survival. Although cancers...states. Ras Ras is a GTPase that is essential for regulating the growth of the cell. p53 p53, the most frequently...Esposito Lab for use with the Gateway® Cloning Platform (Life Technologies™) to permit construction of...

...Cut Base Edit Nick Prime Edit Transcriptional Regulation Activate Interfere Epigenetics RNA Targeting ...the spread of infection throughout the entire population. Type VI enzymes that function in mammalian cells...mammalian systems, Cas13a does not exhibit the collateral RNA degradation seen in bacteria. Want more information...

...Cut Base Edit Nick Prime Edit Transcriptional Regulation Activate Interfere Epigenetics RNA Targeting ...changes, researchers use ssDNA or dsDNA repair templates with 1. homology to the DNA flanking the DSB and...edit close to the gRNA PAM site. When a repair template is present, the cell may repair a DSB using homology-directed...

...Cut Base Edit Nick Prime Edit Transcriptional Regulation Activate Interfere Epigenetics RNA Targeting ...changes, researchers use ssDNA or dsDNA repair templates with homology to the DNA flanking the DSB and ...edit close to the gRNA PAM site. When a repair template is present, the cell may repair a DSB using homology-directed...

...genome engineering. CRISPR Topic Page : Want the latest news on CRISPR? Experts cover CRISPR topics on ...information into a specific DNA site Transcriptional Regulation Screen Activate dCas9 fused to an activator peptide...Purify dCas9 fused to an epitope tag can be used to isolate a given genomic locus. Tag Various CRISPR methods...

...cleavage sites, while the addition of an ssODN donor template can mediate gene replacement by homologous recombination...designed for in vivo transcrition controlled by the regulatory sequences of an RNA polymerase III transcribed...restriction sites. Design oligos based on the following template: example : Resources: Protocol for generating ...

...called γ-retroviruses. γ-Retroviruses can package relatively high amounts of DNA (up to ∼8 kb), and infection... variant that has WRE, a post-transcriptional regulatory element that increases export to the cytoplasm...retroviral plasmid Vignali 27995 TtRMPVIR CMV/MSV Tet-regulated expression of shRNA; expresses rtTA. See Zuber...

...using Protein A or Protein G affinity columns. Formulation Buffer After affinity chromatography, antibodies...exchange using centrifugal columns. The final formulation buffer is phosphate-buffered saline containing...generated. The final antibody concentration is calculated from the standard curve. Using ImageJ software...

...technologies: from genome engineering and gene regulation to fluorescent proteins. Addgene’s collection...seed and other stocks of Arabidopsis thaliana and related species. The Arabidopsis Information Resource (..., computational and experimental resources that relate to the control of gene expression in the grasses...

... Barcoding Libraries Control COVID-19 Research Related Addgene's lentiviruses are made from plasmids that... CMV promoter, Hygro Campeau COVID-19 Research Related Lentivirus Browse our list of available viruses...viruses based on plasmids in our repository that are related to COVID-19 research. ID Name Description PI 141391...

...plasmid ( Addgene #157970 ). Viral preparations are formulated in one of the following three buffers. All buffers...serotype displays highly efficient transduction and lateral spread in the retina following subretinal injection...E531D) serotype has increased transduction and lateral spread in the retina following subretinal injection...

...Cloning & Genomic Tools Metabolism Networks & Gene Regulation Sensing & Signaling Strains Browse Addgene's ...Addgene's collection of synthetic biology related bacterial strains. SynBio Bacterial Strains Search the table...

...Cloning & Genomic Tools Metabolism Networks & Gene Regulation Sensing & Signaling Strains Browse Addgene's ...Addgene's collection of synthetic biology plasmids related to cell-to-cell communications and environmental sensing...

...Cloning & Genomic Tools Metabolism Networks & Gene Regulation Sensing & Signaling Strains Browse Addgene's ...Addgene's collection of synthetic biology plasmids related to metabolic pathways and components. Examples Include...

...Cloning & Genomic Tools Metabolism Networks & Gene Regulation Sensing & Signaling Strains Browse Addgene's ...Addgene's collection of synthetic biology plasmids related to Cloning and Genomic Tools for library construction...

...Keisuke Kaji Jonathan Katz Nathan Lawson Kathrin Plath Miguel Ramalho-Santos Derrick Rossi Michael Ruppert...family of at least 17 DNA binding transcriptional regulators with diverse roles in processes such as cell ...

...Collection Protein Expression Cell Migration Consortium Malate Dehydrogenase CUREs Community (MCC) Collection ...Collection Screening Multiplexed Overexpression of Regulatory Factors (MORF) Collection Synthetic Biology Voigt...