...cytosine base editor that can alter DNA bases without inducing a DNA break. Cytosine base editors convert C...gRNA sequence to direct the dCas9-activator to promoter or regulatory regions of your gene of interest....Empty gRNA Expression Vectors Select a gRNA expression plasmid based on factors such as selectable marker...-FokI Screen Pooled Libraries gRNAs Empty gRNA Vectors gRNAs By Species Mammalian Bacteria Drosophila ...editing window specified by the gRNA. Adenine base editors convert adenine to inosine, which is replaced by...Catalytically dead dCas9 fused to a transcriptional activator peptide can increase transcription of a specific...separate gRNA expression plasmid to target the dCas9-activator to your specific locus. ID Plasmid Gene/Insert...

...support educators interested in integrating MDH-related research into their teaching laboratories. They ...access to mentors, virtual and local professional development, membership to a local hub of educators teaching... Depositor Collections MDH CUREs Community Malate Dehydrogenase...They encourage instructors teaching different types of courses, at any type of institution, and at different...teaching MDH CUREs, and opportunities to mentor future MCC members. Find a regional hub (Link opens in a ...a new window) and contact the hub director for more information. Why MDH? MDH is stable, inexpensive to...cloned into an IPTG-inducible His-tag expression vector. All His-tags are cloned at the C-terminus of ...

... Viral Vector Packaging Service AAV Chemogenetics Viral Vector Packaging Service: Chemogenetics...information about this field. Receptor hM3D(Gq) DREADD rM3D(Gs) DREADD Inhibitory hM4D(Gi) DREADD KOR DREADD...Promoter Synapsin CaMKIIa CD68 Dlx GFAP nEF CAG E2 regulatory element Tag Fusion tags mCherry HA Non-fusion...AAV9 Retrograde AAV PHPeB Clear Filters ID Name Receptor and Activity Reporter/Tag Cre requirement Serotype...pAAV-hSyn-dF-HA-KORD-IRES-mCitrine kappa-opioid receptor (KOR) - Inhibition IRES mCitrine Cre-dependent...Karpova and Schaffer labs. See the Retrograde AAV Inventory Page . Browse all Chemogenetics AAV Don’t See ... eligible AAV plasmids and we’ll make the viral vector prep you need. Or fill out our form to let us know...

...reduced immune effector functions Expression of IgG Monoclonals with Engineered Immune Effector Functions....light-chain DNAs into a single mammalian expression vector A tool kit for rapid cloning and expression of ...1827:313-334. Daniel Christ pComb3 phage display vector system for producing human antibodies Synthetic...Carlos Barbas Variant of the pComb3 phage display vector system for isolation of scFv, diabody and Fab fragment...Methods. 2000 Aug 28;242(1-2):159-81. Carlos Barbas Vector system for expression, dimerisation and detection...antibody fragments in the form of scFvs A simple vector system to improve performance and utilisation of...monoclonal antibodies against zebrafish neural receptors and secreted proteins suitable for wholemount ...

...cellular receptors. The cellular receptor for SARS-CoV-2 and SARS-CoV is hACE2, an angiotensin receptor. Another...Angiotensin Converting Enzyme 2) is the host cell receptor mediating the entry of SARS-CoV and SARS-CoV-2...Cytokines - SARS-CoV-2 infection induces a cytokine storm response in the body. For a full list of cytokines...and open-access SARS-CoV-2 detection assay for laboratory and home testing. Kellner, et al. bioRxiv 2020.06.23.166397... mutants (Timothy Whitehead). SARS-CoV-2 Spike Receptor Binding Domain Deep Mutation Scanning Library ...comprehensive mapping (Jesse Bloom). SARS-CoV-2 Spike Receptor Binding Domain Site-Saturation Mutagenesis Libraries...and Is Blocked by a Clinically Proven Protease Inhibitor . FURIN - Andersen, KG, et al, 2020, Nature Medicine...

...Expression (this is a bicistronic vector rather than a fusion protein vector) ZsGreen1-N1 - Mammalian Expression...93 5.7 10 min Monomer pCS2+mNeonGreen-C Cloning Vector - Mammalian Expression Jump to Top Yellow Protein...5.7 Monomer (A206K) pcDNA3 mCitrine LIC cloning vector (6C) - Mammalian Expression pCS2+8CmCitrine - Zebrafish...Xenopus/C.elegans/Sea urchin pET mCitrine LIC cloning vector (u-mCitrine) - Bacterial Expression mCitrine-N1...562 49 6.5 2.5 hr Monomer pET mOrange LIC cloning vector (u-mOrange) - Bacterial Expression pCS2+8CmOrange... - Mammalian Expression pCS2+mScarlet-C Cloning Vector - Mammalian Expression mScarlet-I 569 593 57 5.4...16 4.5 15 min Monomer pcDNA3 mCherry LIC cloning vector (6B) - Mammalian Expression pLV-mCherry - Mammalian...

...interest. Browse Cancer Research Plasmids by Pathway mTOR mTOR is a protein kinase that anchors a signaling network...Cancer Research Broad Target Accelerator Plasmids: Collection of vectors from the Boehm , Hahn , Meyerson...mutated gene in human cancer, is a transcription factor and tumor suppressor. Wnt Coming soon! Kits and...mutant alleles described in the initial Target Accelerator publications, focused on somatic variants found...pan-cancer” analyses. Cancer Pathways ORFs Kit : Set of vectors from the Sabatini & Wood Labs containing cDNAs ... Lab RAS Pathway Clone Collection 2.0 : Set of vectors from the Esposito Lab for use with the Gateway®...permit construction of RAS pathway gene expression vectors for in vitro and in vivo research. TP53 Mutagenesis...

...shRNA CRISPR gRNAs Depositor Collections A modular set of plasmids from a depositor organized by a common...Collections Addgene Plasmid Collections Addgene's repository contains 156,627 plasmids . We've gathered our...our curated plasmid resources, kits, and depositor collections on one page to help you find what you're...looking for a non-plasmid item, browse our entire repository . Plasmid Resources Our curated resources group...common use. Click here to view all collections. Depositor Collection Types CRISPR Fluorescent Proteins Protein... Protein Expression Synthetic Biology Viral Vectors Communities Find a scientific community that has established...Plasmid RESource (EXPRESs) Consortium Krüppel-like factors (KLF) Plasmid Resource RESOLUTE Plasmid Collection...

... Viral Vector Packaging Service AAV Serotype Testing AAV Viral Vector Packaging Service: ...available from Addgene's viral service. Control EGFP vectors in various serotypes for serotype testing: AAV1...addition to the standard 100 µL sample size, the vectors listed below are also available as 20 µL. The ...experiments. For more information, see our full AAV inventory or our AAV production page . Note: For a list ...100 µL sized sample, please see our Control AAV Inventory . The catalog ID for the 20 µL sized testing sample...does not have the suffix (for example, AAV1). AAV Vectors for Serotype Testing pAAV-CAG-GFP (Plasmid #37825... eligible AAV plasmids and we’ll make the viral vector prep you need. Or fill out our form to let us know...

...Blog: Plasmids 101-Viral Vector Elements Blog: AAV use in CRISPR Viral Vectors 101 eBook Packaging Plasmids...Pseudotyping Want to save time? Check out our Viral Vector Packaging Service and learn about our options for...amplification system Gradinaru 129024 pProvector_AAV2_586_PLGLAR AAV2_586_PLGLAR Recombinant AAV2 rep/... Gradinaru 206504 AAV-KP1 AAV-KP1 AAV packaging vector containing AAV2 rep and KP1 cap and partial AAV2...promoter Kay 206512 AAV-LK03 AAV-LK03 AAV packaging vector containing AAV2 rep and LK03 cap and partial AAV2...promoter Kay 206513 AAV-AM AAV-AM AAV packaging vector containing AAV2 rep and AM cap and partial AAV2...Schaffer 213968 AAV8R-12 Capsid AAV8R12 AAV packaging vector carrying AAV2 rep gene and Cap8R modified AAV8 ...

...viral vectory packaging service. Browse constructs currently available in viral format.... Viral Vector Packaging...Browse In-Stock AAV Adeno-associated virus (AAV) vectors are safe and useful gene delivery tools that effectively... in small volumes, and ready to ship. All viral vector preps are purified by density centrifugation. Addgene...service. You can choose a transfer plasmid from our repository and request packaging in one of five serotypes...or AAVrg). For more information, visit our Viral Vector Packaging Service overview. Function Chemogenetics...Monster, Brainbow Molecular Tools Tetracycline Transactivators, Affinity Purification, Cell Ablation Monosynaptic...

...Lentiviral reporter vector expressing firefly luciferase. pCMV-FLuc - Retroviral reporter vector expressing firefly...Lentiviral vector expressing destabilized EGFP. Lenti-luciferase-P2A-Neo - Lentiviral reporter vector expressing...permissive cells that express ACE2, the cellular receptor for SARS-CoV-2, in a single round of viral entry...-Luc2-IRES-ZsGreen-W - Lentiviral dual reporter vector expressing firefly luciferase and ZsGreen. NL4-...3 mCherry Luciferase - Lentiviral dual reporter vector expressing mCherry and firefly luciferase. pLentiEGFPdestablized...coronaviruses (184 KB) Addgene Blog Posts Viral Vectors 101: Pseudotyping Antibody Neutralization Response...

...gRNA sequence to direct the dCas9-activator to promoter or regulatory regions of your gene of interest....Empty gRNA Expression Vectors Select a gRNA expression plasmid based on factors such as selectable marker...-FokI Screen Pooled Libraries gRNAs Empty gRNA Vectors gRNAs By Species Mammalian Bacteria Drosophila ...Catalytically dead dCas9 fused to a transcriptional activator peptide can increase transcription of a specific...separate gRNA expression plasmid to target the dCas9-activator to your specific locus. ID Plasmid Gene/Insert...both the gRNA and Cas protein act as all-in-one vectors, but their function is often limited to a single...

... Viral Vector Packaging Service AAV Monosynaptic Neuronal Tracing Viral Vector Packaging Service... Addgene does not distribute rabies virus. AAV Vectors for Monosynaptic Neuronal Tracing ID Name Description...specifically into cells that express the TVA viral receptor. Thus, by pseudotyping the rabies virus with EnvA...specifically targets the starter cell via the EnvA receptor. RABV is able to replicate in the starter cell...free to infect neighboring cells via G-protein receptors, which are naturally expressed at presynaptic ... eligible AAV plasmids and we’ll make the viral vector prep you need. Or fill out our form to let us know...format. Please note this does not guarantee viral vector packaging service, but lets us know what viruses...

...variable 91,948 Toronto KnockOut - Version 1 1000000069 Knockout Human Moffat 3rd 12 176,500 Toronto KnockOut...-FokI Screen Pooled Libraries gRNAs Empty gRNA Vectors gRNAs By Species Mammalian Bacteria Drosophila ...3rd 10 187,535 Advanced Catalogue of Epigenetic Regulators (ACER) 226117 Knockout Human Lu 3rd 10 8,205 ...Knockout Mouse Tolar 3rd ~4 78,637 Mouse Chromatin Regulator Library 200011 Knockout Mouse Griffin, Manguso...119977 Knockout Human Wei 3rd Varies 126,773 Human mTORC1 Focused Library 164084 Knockout Human Sabatini ...TP53 Exon Mutation Libraries 229137–229140 Donor Vector Human Stiewe N/A Varies 1,910–3,034 per exon Human...Superti-Furga 3rd Varies Varies Human Transcription Factor Knockout Library 162275 Knockout Human Li 3rd ~...

...gRNA sequence to direct the dCas9-activator to promoter or regulatory regions of your gene of interest....Empty gRNA Expression Vectors Select a gRNA expression plasmid based on factors such as selectable marker...-FokI Screen Pooled Libraries gRNAs Empty gRNA Vectors gRNAs By Species Mammalian Bacteria Drosophila ...Catalytically dead dCas9 fused to a transcriptional activator peptide can increase transcription of a specific...separate gRNA expression plasmid to target the dCas9-activator to your specific locus. ID Plasmid Gene/Insert...both the gRNA and Cas protein act as all-in-one vectors, but their function is often limited to a single...

... Viral Vector Packaging Service AAV Optogenetics Viral Vector Packaging Service: Optogenetics...quality AAV preps from select plasmids in the repository. Browse our optogenetics AAV collection, including...plasmids available in Addgene's collection. Opsin Excitatory Wild-type ChR2 ChR2/H134R soCoChR ChR2/other ... Chrimson, ChRmine, ChroME, ChETA, C1V1 (t/t) Inhibitory Arch and variants eNpHR and variants Jaws stGtACR...Promoter CAG CaMKII CBA Dlx EF1a/nEF Synapsin E2 regulatory element Fluorophore GFP Red-wavelength fluorescent...Karpova and Schaffer labs. See the Retrograde AAV Inventory Page . Don’t See What You’re Looking For? Our ... eligible AAV plasmids and we’ll make the viral vector prep you need. Or fill out our form to let us know...

...fluorophores, excitatory and inhibitory opsins , genetically-encoded calcium indicators, and rabies targeting... Depositor Collections INTRSECT Plasmid Collection INTRSECT...INTRSECT (intronic recombinase sites enabling combinatorial targeting) is a synthetic molecular targeting...Fon-sRGECO Flp AND NOT Cre Dual recombinase-dependent: Excitatory Opsins Addgene ID Plasmid Logic Sites and Mutations...ChRmine-oScarlet Flp AND NOT Cre Dual recombinase-dependent: Inhibitory Opsins Addgene ID Plasmid Logic Sites and Mutations...Deisseroth K. 2014. Targeting cells with single vectors using multiple-feature Boolean logic. Nat Methods...Krook-Magnuson E. 2019. A novel population of long-range inhibitory neurons. Preprint (Link opens in a new window...

... Viral Vector Packaging Service AAV Controls Viral Vector Packaging Service: Control...quality AAV preps from select plasmids in the repository. Browse our control AAV collection, including...8 Deisseroth 135630 pAAV-S5E2-dTom-nlsdTom E2 regulatory dTomato Constitutive 1, 9, PHP.eB Dimidschstein...Dimidschstein 135631 pAAV-S5E2-GFP-fGFP E2 regulatory EGFP Constitutive 1, 9, PHP.eB Dimidschstein 137135 pAAV-...Karpova and Schaffer labs. See the Retrograde AAV Inventory Page . Don’t See What You’re Looking For? Our ... eligible AAV plasmids and we’ll make the viral vector prep you need. Or fill out our form to let us know...format. Please note this does not guarantee viral vector packaging service, but lets us know what viruses...

...//www.thesgc.org/ SGC Toronto: https://www.thesgc.org/scientists/groups/toronto SGC Oxford: http://www.sgc.ox.ac.uk...without restriction. Many SGC plasmids from the Toronto, Oxford and Stockholm sites have been deposited...the SGC plasmid collection by All Plasmids SGC Toronto SGC Oxford SGC Stockholm Bacterial Expression Plasmids...Thrombin cleavage, Bac-to-Bac Baculovirus Expresion vector 26117 pNIC-CH EF199843 Hexahistidine tag, AmpR ...cleavage, AmpR 26095 pFHMSP-LIC-N N/A Expression vector for secreted proteins with N-terminal hexahistidine... System, AmpR 26100 pFHMSP-LIC-C N/A Expression vector for secreted proteins with C-terminal hexahistidine...