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Showing: 61 - 80 of 110 results

  1. CRISPR Plasmids - Parasites

    Type
    Collection
    ...Libraries gRNAs Empty gRNA Vectors gRNAs By Species Mammalian Bacteria Drosophila Plant C. elegans Yeast Zebrafish...disease in the world. Parasites such as Plasmodium (malaria), Toxoplasma (toxoplasmosis), Trypanosoma (African...

  2. Fluorescent Protein Guide: In Vivo Imaging

    Type
    Collection
    ...protein-protein interactions in deep-tissue organs and whole mammals. Genetically encoded fluorescent probes are suitable...spatially selective imaging of tissues in living animals. Further development of the original iRFP has resulted...

  3. Plasmid Collections

    Type
    Collection
    ...Empty Backbones Genetic Code Expansion Immunology Mammalian RNAi Microbiology Plant Expression Stem Cells ... with the same backbone and only differing in a small region. Click here to view all pooled libraries....

  4. Bikard Lab - CRISPR Repression Collection

    Type
    Collection
    ...bioRxiv preprint . Detailed information about chromosomal integration of pLC97 can be found in this article...Shearwin, K. E. (2013). One-Step cloning and chromosomal integration of DNA. ACS Synthetic Biology, 2(...

  5. Resolute Plasmid Collection

    Type
    Collection
    ...academic research as well as applied research in small biotech and pharmaceutical companies. The RESOLUTE... fluorescence, mass/charge properties, protein thermal stabilization, and changes of voltage all contribute...

  6. Synthetic Biology - Overview

    Type
    Collection
    ...Plasmids by Model Organism Bacterial Plant Yeast Mammalian Worm Fungal Algal Featured SynBio Deposits Alper...Balazsi Lab Negative Autoregulation and Yeast-Mammalian Circuit Transfer C. Collins Lab Quorum Sensing...

  7. Adenovirus Plasmids

    Type
    Collection
    ...recombination of adenovirus plasmids in either mammalian cells or microorganisms, including bacteria and...This DNA molecule can then be transfected into mammalian packaging cell lines to generate adenovirus particles...

  8. Retrovirus Plasmids

    Type
    Collection
    ...inducible expression of GFP T2A Cre fusion in mammalian cells Foijer 47916 pRXTN CMV/MSV pRXTN is a modified...-Luc MoMSV Stable expression of luciferase in mammalian cells Wong Return to Top Do you have suggestions...

  9. Antibody Production

    Type
    Collection
    ...Production For recombinant antibodies (rAb) encoded by mammalian expression vectors, human embryonic kidney cells...target antigen is first transiently expressed in mammalian cells. To learn which specific QC experiments ...

  10. CRISPR Plasmids - Yeast

    Type
    Collection
    ...Libraries gRNAs Empty gRNA Vectors gRNAs By Species Mammalian Bacteria Drosophila Plant C. elegans Yeast Zebrafish... C->T (or G->A on the opposite strand) within a small editing window specified by the gRNA. Adenine base...

  11. mTOR Pathway

    Type
    Collection
    ...mTOR Gene List Resources The m echanistic or m ammalian t arget o f r apamycin (mTOR) is a key metabolic... enriched in brain S6K Also known as RPS6KB1; Ribosomal protein S6 kinase B1 TSC1/2 TSC1 TSC2 Tuberous...

  12. Qi Lab CRISPR Page

    Type
    Collection
    ...CRISPR system for genome mutagenesis ( Cong et al. , Mali et al. , Jiang et al. , Hwang et al. ), is that ...targeted genes. 2. Two-plasmid CRISPRi system for mammalian gene knockdown The first plasmid ( pdCas9_humanized...

  13. AAV Packaged on Request

    Type
    Collection
    ...delivery tools that effectively transduce many mammalian cell types. AAV is used in a wide range of experiments...Quantity Total Volume Minimum Titer Typical Titer Small 10 × 100 µL aliquots 1 mL 4 × 10 12 GC/mL* 1 × 10...

  14. CRISPR Plasmids - Bacteria

    Type
    Collection
    ...Libraries gRNAs Empty gRNA Vectors gRNAs By Species Mammalian Bacteria Drosophila Plant C. elegans Yeast Zebrafish... C->T (or G->A on the opposite strand) within a small editing window specified by the gRNA. Adenine base...

  15. TALEN Guide

    Type
    Collection
    ...Intuitively, one would expect that assembling relatively small, repeat-laden DNA regions into a single construct...sequence-specific TAL effectors for modulating mammalian transcription. Nat Biotechnol. 2011 Feb;29(2):...

  16. Viral Vectors

    Type
    Collection
    ...into the host genome. AAVs are limited by their small packaging capacity. Adenovirus Plasmids | Guide ...delivery of genetic information to hard-to-transfect mammalian cells. In addition, "viral pseudotyping" can be...

  17. CRISPR Plasmids - Plants

    Type
    Collection
    ...Libraries gRNAs Empty gRNA Vectors gRNAs By Species Mammalian Bacteria Drosophila Plant C. elegans Yeast Zebrafish... C->T (or G->A on the opposite strand) within a small editing window specified by the gRNA. Adenine base...

  18. Viral Production

    Type
    Collection
    ...properties of the final product, particularly in large animal studies. Endotoxin contamination is minimized by...MRP 30 kDa subunit (RPP30) as a control for normalization purposes are being used. Quality Control Mycoplasma...

  19. Lentivirus Plasmids

    Type
    Collection
    ...gene encoded by the transfer plasmid is toxic to mammalian cells, it can be difficult to efficiently produce...gateway destination plasmid for cDNA expression Ramalho-Santos 24129 pULTRA 3rd bi-cistronic expression...

  20. CRISPR Plasmids - Tagging

    Type
    Collection
    ...Libraries gRNAs Empty gRNA Vectors gRNAs By Species Mammalian Bacteria Drosophila Plant C. elegans Yeast Zebrafish...tagging of proteins expressed from their natural chromosomal context. These CRISPR tagging methods allow for...
Showing: 61 - 80 of 110 results