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Showing: 61 - 80 of 174 results

  1. CRISPR Plasmids - Double-Strand Break (Cut)

    Type
    Collection
    ...the tables below for CRISPR plasmids designed to introduce a DSB. Plasmids are available for expression...Mammalian ID Plasmid Gene/Insert Promoter Selectable Marker PI Publication Bacteria ID Plasmid Gene/Insert...Drosophila ID Plasmid Gene/Insert Promoter Selectable Marker PI Publication Plant ID Plasmid Gene/Insert... elegans ID Plasmid Gene/Insert Promoter Selectable Marker PI Publication Yeast ID Plasmid Gene/Insert...Zebrafish ID Plasmid Gene/Insert Promoter Selectable Marker PI Publication Xenopus ID Plasmid Gene/Insert...suggestions for other plasmids that should be added to this list? Fill out our Suggest a Plasmid form or e-mail...CRISPR plasmids that create double strand breaks in DNA. CRISPR...

  2. CRISPR Plasmids - Single-Strand Break (Nick)

    Type
    Collection
    ...search the tables below for CRISPR nickase plasmids. Plasmids are available for expression in mammalian...Mammalian ID Plasmid Gene/Insert Promoter Selectable Marker PI Publication Bacteria ID Plasmid Gene/Insert...Drosophila ID Plasmid Gene/Insert Promoter Selectable Marker PI Publication Plant ID Plasmid Gene/Insert...suggestions for other plasmids that should be added to this list? Fill out our Suggest a Plasmid form or e-mail...CRISPR nickase plasmids for the generation of single-strand breaks. CRISPR... CRISPR Nick CRISPR Plasmids: Nick Browse...Browse CRISPR Plasmids By Function Genome Editing Cut Base Edit Nick Prime Edit Transcriptional Regulation...

  3. Allen Institute for Cell Science Plasmid Collection

    Type
    Collection
    ...mEGFP NA Cytoplasm 114405 ATP2A2-mEGFP AICSDP-41 mEGFP SERCA2 Sarcoplasmic reticulum/endoplasmic reticulum...Institute for Cell Science Plasmid Collection Allen Institute for Cell Science Plasmid Collection You May Also...full description of how to use these plasmids, check out Plasmids for Endogenous Gene Tagging in Human...Allen Institute for Cell Science has deposited plasmids to generate fluorescently tagged proteins of key...Fluorescent Protein Guide Subcellular Localization Plasmids Protocols The Allen Institute for Cell Science...induced pluripotent stem cell (hiPSC) lines. The plasmids used to generate these lines have been deposited...distribution to the research community. With these plasmids researchers will be able to generate fluorescently...

  4. Michael J Fox Foundation (MJFF) Plasmid Collection

    Type
    Collection
    ... Plasmid Collections MJFF Parkinson's Plasmid Resource Michael J. Fox Foundation Parkinson's Disease...Disease Plasmid Resource MJFF Plasmids Shared Research Tools (Link opens in a new window) The Michael J.... Michael J. Fox Foundation Plasmids MJFF has created a number of plasmids for expression of variants ...Browse plasmids expressing genes relevant to Parkinson's disease created by the Michael J. Fox Foundation...interest. Browse or search the table below. ID Plasmid Gene/Insert Mutations Industry Return to top MJFF... Addgene. Browse or search the table below. ID Plasmid Description Gene/Insert Industry PI Return to top...

  5. iPSC Neurodegenerative Disease Initiative Plasmid Collection

    Type
    Collection
    ...phenotyping, and distribution efforts. Plasmids The following plasmids can be used to create cell lines with...Find plasmids generated by the iPSC Neurodegenerative Disease Initiative (iNDI) here. Depositor...Also Like... CRISPR Guide CRISPR Protein Tagging Plasmids for Stem Cell Research Neurodegeneration Research... with the research community. To this end, all plasmids that result from the project efforts will be made...made available for research purposes in the iNDI plasmid collection at Addgene. Figure 1: Schematic of iNDI... cell types, as noted in the purpose field: ID Plasmid Description Additional Resources iNDI (Link opens...

  6. Viral Prep Service for Plasmids and Pooled Libraries

    Type
    Collection
    ...the right to use the original plasmid. Distribution of the original plasmid is important because it is the...Overview of Addgene's viral preparation service for plasmids and pooled libraries. Viral preparation options...produces high-quality viral vector preps from select plasmids in our collection. We know that viral vector production... production is a common step after requesting plasmids from Addgene, and by providing this service, we...options. Select from a vast catalog of eligible AAV plasmids and we’ll make the viral vector prep you need....a sample of the purified DNA from the original plasmid that was used to make the virus. In order for Addgene...inclusion is what gives you permission to use the plasmid....

  7. Empty Backbones - Choosing Your Perfect Plasmid Backbone

    Type
    Collection
    ... Plasmid Collections Empty Backbones Choosing Your Perfect Plasmid Backbone Background... Plasmids - More LIC bacterial cloning vectors pTD plasmid ... plasmids Fly UAS, MT Targeted Gene Expression - Rubin lab plasmids for drosophila...of your cells will take up your plasmid. Thus, many plasmids have markers on them so that you...collection of plasmids for packaging and expression. See our dedicated lentiviral plasmid page . ... plasmid for mammalian gene silencing pLVTHM - lentiviral plasmid for mammalian...suggestions for other plasmids that should be added to this list? Fill out our Suggest a Plasmid form or e-mail...

  8. New England Biolabs Cell-Imaging Plasmid Collection

    Type
    Collection
    ...Cell-Imaging Plasmid Collection New England Biolabs Cell-Imaging Plasmid Collection Technology Plasmids Supplemental...Biolabs has deposited plasmids at Addgene to generate SNAP, CLIP, ACP or MCP tagged plasmids for visualization...synthases needed for use of these tags. Plasmids Addgene ID Plasmid Substrate Feature 101137 pSNAP-tag (T7...Control Plasmid SNAP-tag Control for mitochondrial localization 101124 pSNAPf-H2B Control Plasmid SNAP-tag...Control Plasmid CLIP-tag Control for nuclear localization 101130 pCLIPf-Cox8A Control Plasmid CLIP-tag...Control Plasmid ACP/MCP-tag Control for cell surface localization 101131 pACP-GPI Control Plasmid ACP/MCP-tag... of proteins inside living or fixed cells. The plasmids associated with this technology are available ...

  9. Joung Lab CRISPR-Cas/RNA-Guided Nuclease (RGN) Plasmids

    Type
    Collection
    ...CRISPR-Cas/RGN expression plasmids for use in human cells CRISPR-Cas/RGN expression plasmids for use in zebrafish...Guide RNA expression plasmids for the EGFP Reporter Gene Guide RNA expression plasmids for endogenous human...RNA-Guided Nuclease (RGN) Plasmids Genome... Genome Engineering CRISPR Joung Lab CRISPR Plasmids Joung Lab CRISPR-Cas...CRISPR-Cas/RNA-Guided Nuclease (RGN) Plasmids You may also like... CRISPR Guide CRISPR Protocols gRNA Design Tools...Joung Lab CRISPR-Cas/RGN reagents: Cpf1 expression plasmids (AsCpf1 and LbCpf1) for use in mammalian cells...human genes Guide RNA expression plasmids for endogenous zebrafish genes...

  10. Bacterial Expression Systems

    Type
    Collection
    ...Reporter Plasmids You may also like… Plasmids 101 Addgene's Molecular Biology Guides Plasmids 101: E. ...interest. Plasmid ID Promoter Inducer(s) PI Purpose Murray Lab pBEST plasmids Various Plasmids pBAD, OR2...Purification Controlled Expression Reporter Plasmids Reporter Plasmids Tagging and Visualization Purification...list of our bacterial CRISPR plasmids, see here . pGRG Series Various Plasmids Tn7 Transposition Nancy Craig... to the periplasm. Series contains vectors with a YFP variant that functions in the periplasm and can ...green light. BIOFAB plasmid set 1000000037 N/A N/A Drew Endy This kit contains plasmids with various combinations...specific level. Return to Top Reporter Plasmids Reporter plasmids can be used to detect events and molecules...

  11. Zhang Lab CRISPR Page

    Type
    Collection
    ...Barretto R, Habib N, Hsu PD, Wu X, Jiang W, Marraffini LA, Zhang F. Science . 2013 Jan 3. DOI: 10.1126/science..., Fine EJ, Wu X, Shalem O, Cradick TJ, Marraffini LA, Bao G, Zhang F. Nat Biotechnol. 2013 Sep;31(9):827...tracrRNA: This plasmid contains three expression cassettes. To target a given site, the plasmid can be digested...adenocarcinoma pathology. These plasmids as well as a backbone plasmid for cloning new targets are described...sgRNA backbone for cloning new targeted plasmids. The plasmid can be digested using SapI, which will reveal...

  12. COVID-19 Resources

    Type
    Collection
    ...sequences. SARS-CoV Plasmids : Plasmids that are available containing SARS-CoV (SARS classic) sequences. Mammalian...nucleocapsid protein (Brian Geiss). Plasmids SARS-CoV-2 Plasmids Many plasmids containing SARS-CoV-2 sequences...Addgene will maintain this plasmid collection page, which highlights plasmids available that may be useful...SARS-CoV-2 nucleocapsid protein. SARS-CoV-2 Plasmids : Plasmids that are available or coming soon containing...to popular envelope and packaging plasmids. Ginkgo Bioworks Plasmid Collection - Addgene has partnered...Bioworks to distribute SARS-CoV-2 plasmids. The collection contains: Plasmids for E. coli and Yeast expression...available Ginkgo SARS-CoV-2 plasmids are curated on a separate Ginkgo Bioworks Plasmid Collection page. SARS-CoV...

  13. Penn Vector Core Partnership with Addgene

    Type
    Collection
    ...GENIE, Douglas Kim AV-1-PV2817 100839-AAV1 pAAV.CAG.Flex.GCaMP6m.WPRE.SV40 Biosensor GENIE, Douglas Kim ...GENIE, Douglas Kim AV-1-PV2819 100833-AAV1 pAAV.Syn.Flex.GCaMP6f.WPRE.SV40 Biosensor GENIE, Douglas Kim ...GENIE, Douglas Kim AV-1-PV2821 100845-AAV1 pAAV.Syn.Flex.GCaMP6s.WPRE.SV40 Biosensor GENIE, Douglas Kim ...GENIE, Douglas Kim AV-1-PV2823 100841-AAV1 pAAV.Syn.GCaMP6m.WPRE.SV40 Biosensor GENIE, Douglas Kim AV-...GENIE, Douglas Kim AV-1-PV2833 100844-AAV1 pAAV.CAG.GCaMP6s.WPRE.SV40 Biosensor GENIE, Douglas Kim AV-...GENIE, Douglas Kim AV-1-PV3081 100836-AAV1 pAAV.CAG.GCaMP6f.WPRE.SV40 Biosensor GENIE, Douglas Kim AV-...GENIE, Douglas Kim AV-1-PV3846 100857-AAV1 pAAV.Syn.NES-jRGECO1b.WPRE.SV40 Biosensor GENIE, Douglas Kim ...

  14. Worm Expression Resources

    Type
    Collection
    ... Plasmid Collections Worm Plasmids Worm Plasmids and Resources Plasmid Tools Worm Constructs...some of the plasmids and tools available at Addgene for use in worms. Browse Plasmids Plasmid Tools for ...complete list of plasmids that can be used in worms, search Addgene's entire collection. ID Plasmid Description... Gene/Insert PI Plasmids Expressing Worm Genes/Inserts The table below lists plasmids that contain a worm...sequence: ID Plasmid Gene/Insert PI Don’t see your gene listed? Know of some great plasmid tools for the... tools , or by suggesting plasmids for the collection. Return to top Plasmid Kits and Collections Kits... promoter. cGAL and Split cGAL plasmids - Paul Sternberg Lab. Plasmids for the temperature-robust GAL4...

  15. Rinehart Lab Phosphoprotein Reagents

    Type
    Collection
    ...SepOTSγ Plasmid 68285 SepOTSδ Plasmid 68286 SepOTSϵ Plasmid 68287 SepOTSζ Plasmid 68288 SepOTSη Plasmid...SepOTSθ Plasmid 68290 SepOTSι Plasmid 68291 SepOTSκ Plasmid 52054 SepOTSµ ( aka. B40 OTS) Plasmid 68294 ...68294 SepOTSν Plasmid 68295 GFP E17TAG Plasmid 68296 GFP S2TAG Plasmid 68297 GFP Q157TAG Plasmid 68298 GFP...Amp) Plasmid 68300 MBP-MEK1 Plasmid 68301 MBP-MEK1 S218TAG Plasmid 68302 MBP-MEK1 S222TAG Plasmid 68305... 68306 C321.ΔA.Δserb.Amp S Plasmid 68292 SepOTSλ Plasmid 68307 SupD Plasmid 34623 SepOTSα SepRS/EF-Sep...pKD-SepRS-EFSep) Plasmid 34624 SepOTSα tRNA-Sep ( aka. pCAT112TAG-SepT) Plasmid 68283 SepOTSβ Plasmid 68284 SepOTSγ...( 188537 ) is a ROP minus plasmid and is not compatible with other plasmids containing high copy ColE1...

  16. Microbiology Resources

    Type
    Collection
    ... Plasmid Collections Microbiology Plasmids Microbiology Plasmids and Resources Addgene...histolytica Leishmania sp. Plasmodium sp. Toxoplasma gondii Trypanosoma sp. Plasmids for Viruses Species Dengue...Addgene Microbiology Resources Plasmid Kits for Microbiology : Sets of plasmids for fluorescence, cloning ...guide on assembly standards. Browse Plasmids by Microbe System Plasmids for Archaea Species Haloferax sp....Vibrio sp. Yersinia pestis Plasmids for Cyanobacteria Cyanobacteria Plasmids for Fungi Species Aspergillus...Aspergillus sp. Cryptococcus sp. Plasmids for Prions Prions Plasmids for Protozoa Species Entamoeba histolytica... tools , or by suggesting plasmids for the collection. Return to top Plasmid Kits for Microbiology Kits...

  17. Viral Vectors

    Type
    Collection
    ...for select plasmids are now available through Addgene, learn more here . Browse Viral Plasmids by Type Visit... to find plasmids that can be used to engineer various types of viruses. Lentivirus Plasmids | Guide Lentiviruses...transgene expression. Overview of Viral Plasmids While plasmids are typically used to encode, propagate...organism. Figure 1. Using viral plasmids to generate virus. A) Viral plasmids have been engineered such that...genomes have been adapted into a plasmid-based technology, such that plasmids can be used to create viruses...researchers can introduce plasmids to a host to generate virus. Furthermore, these plasmids can be modified to...are usually divided among many plasmids, such that all of these plasmids must be present for a viral particle...

  18. Fluorescent Protein Guide: Biosensors

    Type
    Collection
    ... Biosensors Luciferase Plasmids Subcellular Localization Optogenetics Plasmids Viral Service: AAV Biosensors...suggestions for other plasmids that should be added to this list? Fill out our Suggest a Plasmid form or e-mail...Collection of plasmids encoding fluorescent biosensors to measure biomolecules or genes via FRET or other... Plasmid Collections Fluorescent Proteins Fluorescent Biosensors Fluorescent Proteins: Biosensors...characterization (e.g. in vitro vs. in vivo , cytoplasmic vs. extracellular vs. organelle-localized). Browse...our catalog . Use the article links to find the plasmids associated with the article. We also offer ready-to-use...ready-to-use AAV preparations of select plasmids; these are noted with the viral service icon . Metal Ions...

  19. Fluorescent Protein Guide: Subcellular Localization

    Type
    Collection
    ...ER-mNeonGreen Endoplasmic Reticulum KDEL sequence mNeonGreen Dorus Gadella 137805 ER-mScarletI Endoplasmic Reticulum...ERmoxGFP Endoplasmic Reticulum ER retention signal moxGFP Erik Snapp 68126 ERoxBFP Endoplasmic Reticulum...BFP-KDEL Endoplasmic Reticulum BiP ER signal sequence BFP Gia Voeltz 61807 Rtn4a-GFP Endoplasmic Reticulum...mCh-Sec61 beta Endoplasmic Reticulum Sec61 mCherry Gia Voeltz 49154 BFP-Sec61 beta Endoplasmic Reticulum Sec61..._N1 Endoplasmic Reticulum CytERM mScarlet-I Dorus Gadella 85067 pCytERM_mScarlet-H_N1 Endoplasmic Reticulum...suggestions for other plasmids that should be added to this list? Fill out our Suggest a Plasmid form or e-mail...Plasmids encoding fluorescent proteins tagged with genes or peptides with known subcellular localization...

  20. Tetracycline Inducible Expression

    Type
    Collection
    ...Browse Tetracycline Plasmids Tet-regulated Lentiviral RNAi Vectors Curated Plasmid Collections Background...interest tTA expression plasmid Components for a tetracycline on system are: Plasmid containing a tetracycline-dependent...generation lentiviral plasmid for inducible expression of shRNA; neomycin selection; plasmid 21915 has puromycin...tetraycline system. ID Plasmid Description Element On or off PI 26429 pLenti CMV rtTA3 Blast (w756-1) Lentiviral...CMV promoter, and Blasticidin; can be used to make cell lines Tet-On Advanced; Plasmid 26730 has hygro ...suggestions for other plasmids that should be added to this list? Fill out our Suggest a Plasmid form or e-mail... Plasmid Collections Tetracycline (Tet) Inducible Expression...
Showing: 61 - 80 of 174 results