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Showing: 61 - 80 of 91 results

  1. CRISPR Plasmids - Parasites

    Type
    Collection
    ...gRNA-defined target sequence. DSBs are preferentially repaired in the cell by non-homologous end joining (NHEJ...present, the cell may repair a DSB using homology-directed repair (HDR) instead of NHEJ. In most experimental...

  2. Brzezinski Lab CRISPR Collection

    Type
    Collection
    ...that utilize Golden Gate cloning to insert the desired guide RNA sequence by digestion of the backbone...Otx2 expression in the developing mouse retina requires a unique enhancer and either Ascl1 or Neurog2 ...

  3. TALEN Engineering

    Type
    Collection
    ... engineering TAL effector repeat arrays of any desired length: the REAL (Restriction Enzyme And Ligation...assembly process or lists of plasmid ID numbers required for FLASH assembly. Links to additional pages ...

  4. CRISPR Plasmids - Base Edit

    Type
    Collection
    ... not exist in nature, but have been created by directed evolution of the Escherichia coli TadA, a tRNA...wider editing window, and are thus well suited to directed evolution applications. Examples of these base...

  5. TALEN Plasmids and Kits

    Type
    Collection
    ...higher than the parental vectors. pC-GoldyTALEN directs expression of TALENs from a truncated CAGs promoter...domains (DD, RR) used are obligate heterodimers that require cloning of left and right TALEN monomer proteins...

  6. CRISPR Plasmids - Tagging

    Type
    Collection
    ...Multiple gRNA plasmids may be used. The HDR (homology directed repair) donor plasmid with homology arms and selection...Rep. 2015 . Alternatively, cDNAs can be cloned directly into this vector and targeted to the AAVS1 genomic...

  7. Genomic Deletions in Mammalian Cell Lines

    Type
    Collection
    ... culture. Prep size may be scaled based on the required DNA yield. Perform maxi-prep for each CRISPR/Cas9...step 6 ). Select the clones identified with the desired deletion and move to larger plate or flask for ...

  8. Zhang Lab CRISPR Page

    Type
    Collection
    ...mutations in p53 and Lkb1 , as well as homology directed repair-mediated Kras G12D mutations, leading to...This plasmid also contains a Kras G12D homology directed repair donor template. #60225 - AAV:ITR-U6-sgRNA...

  9. CRISPR Plasmids - dCas9-FokI

    Type
    Collection
    ...Two unique gRNAs, binding ~15-25 bp apart, are required for dCas9-FokI to dimerize at a given region of...

  11. CRISPR Plasmids - Epigenetics

    Type
    Collection
    ...CRISPR Activate and Interfere technologies also indirectly modify local chromatin state. Want more information...

  12. CRISPR Plasmids - RNA Editing

    Type
    Collection
    ... is an A->G change in RNA. dPspCas13b does not require a Protospacer Flanking Sequence (PFS), making it...

  16. Plasmid Collections

    Type
    Collection
    ... are looking for a non-plasmid item, browse our entire repository . Plasmid Resources Our curated resources...

  17. CRISPR Plasmids - gRNAs

    Type
    Collection
    ...wiki entries describing enzyme activity, PAM requirements, experimental considerations, and more, created...
Showing: 61 - 80 of 91 results