...transcription. Epigenetics dCas9 fused to an epigenetic modifier alters chromatin structure/gene expression...Activate dCas9 fused to an activator peptide activates gene expression. Interfere dCas9 alone, or fused to a...factors. gRNAs gRNA plasmids to target specific genes....CRISPR? Experts cover CRISPR topics on Addgene's blog. Addgene's Viral Service : Request ready-to-use viral...Cas enzymes generate double strand breaks (DSBs). Nick Mutated Cas nickase enzymes generate single-strand...CRISPR plasmids available at Addgene. Search by species and CRISPR function. CRISPR...technologies are continously developing and expanding. Addgene works with leading scientists in the field to ensure...

...Mammalian ID Plasmid Gene/Insert Promoter PI Publication Bacteria ID Plasmid Gene/Insert Promoter PI Publication... Publication Plant ID Plasmid Gene/Insert Promoter PI Publication Last reviewed on: January 30, 2025 Do...Transcriptional Regulation Activate Interfere Epigenetics RNA Targeting RNA Targeting RNA Editing Other...large genomic deletions. Compared to deletions generated by Cas9, large deletions created using Cas3 have...out our Suggest a Plasmid form or e-mail help@addgene.org to help us improve this resource!...

...Endogenous Zebrafish Genes TALENs for Endogenous Human Genes TALENs for the EGFP Reporter Gene Additional Plasmids...transcriptional activator target sites and then generates individual user-friendly graphical roadmaps for...

...plasmids targeting 48 sites in the EGFP reporter gene....EGFP Engineered TALENs for the EGFP Reporter Gene The Joung Lab recently used their high-throughput FLASH...TALENs targeted to 48 sites in the EGFP reporter gene ( Reyon & Tsai et al., Nat Biotechnol. 2012 ). The...their target sites on an integrated EGFP reporter gene in cultured U2OS cells. EGFP TALEN Plasmid Plasmid...

...constitutive overexpression of FPs and tagging of genes of interest with FPs in yeast. Characterization ... PGK1 promoters and the KanMX marker. ID Plasmid Gene/Insert Selectable Marker Publication Additional ... Vickers CE, Bydder SF, Zhou Y, Nielsen LK. Dual gene expression cassette vectors with antibiotic selection...

...long or short list of genes provided by the user. Read more about it on the Addgene blog . Eukaryotic Pathogen...identify positively or negatively selected sgRNAs, genes, or pathways. CrispyCrunch CrispyCrunch is used ... genomes (200+) that can predict gRNA targets in gene families, HDR oligonucleotide design, and batch ...sgRNA design tool for genome editing, as well as gene regulation (repression and activation). Genome support...target sites by off-target quality. Validated for gene inactivation, NHEJ, and HDR. Reference genomes include... to identify novel gRNA target sites in a single gene, as well as a target site common to a set of similar...Browse Addgene’s curated list of CRISPR resources and references, including gRNA design tools, CRISPR...

...Metabolism Networks & Gene Regulation Sensing & Signaling Strains Browse Addgene's collection of synthetic...plasmids available from this depositor's lab. Plasmid Gene/Insert Vector Type Promoter Tags PI Publication ...out our Suggest a Plasmid form or e-mail help@addgene.org to help us improve this resource!...

...Metabolism Networks & Gene Regulation Sensing & Signaling Strains Browse Addgene's collection of synthetic...available from this depositor's lab. Plasmid Description Gene/Insert Antibiotic Resistance PI Publication Back...out our Suggest a Plasmid form or e-mail help@addgene.org to help us improve this resource!...

...Metabolism Networks & Gene Regulation Sensing & Signaling Strains Browse Addgene's collection of synthetic...plasmids available from this depositor's lab. Plasmid Gene/Insert Vector Type Promoter PI Publication Back ...out our Suggest a Plasmid form or e-mail help@addgene.org to help us improve this resource!...

...a diverse set of wild-type malate dehydrogenase genes and watermelon mutant clones for your course-based...interactions. Plasmids A diverse set of wild-type MDH genes from several species and mutant clones from watermelon..., or "subunit interface") ID Plasmid Description Gene/Insert Mutations Additional Resources MDH CUREs ...

...contains pre-assembled genetic circuits such as logic gates and higher level gene networks. Sensing and... pathways and auxiliary components. Networks and Gene Regulation Browse plasmids containing both naturally...Lab RNA Feedback Lu Lab Multiplexed/Programmable Gene Networks Mascher Lab Bacillus BioBrick Box Mukhopadhyay...about synthetic biology resources available at Addgene. Browse featured collections or depositing labs...engineering of biological systems for novel functions. Addgene is working with leading synthetic biologists around...facilitate the sharing of parts and information. Addgene can help you to share your synthetic biology plasmids...signaling and environmental sensing. Strains Browse Addgene's collection of engineered bacterial strains, including...

...Arrays for Zebrafish Genes - Browse pre-constructed zinc fingers targeting specific genes Oligomerized Pool...Keith Joung and Daniel Voytas have deposited at Addgene various reagents for engineering and expressing...

...Metabolism Networks & Gene Regulation Sensing & Signaling Strains Browse Addgene's collection of synthetic...available from this depositor's lab. Plasmid Description Gene/Insert Vector Type PI Publication Back to Top Shuttle...out our Suggest a Plasmid form or e-mail help@addgene.org to help us improve this resource!...

...your gene fused to eGFP expression Clevers 18760 MSCV IRES Luciferase MSCV Plasmid for transgene expression...transfer plasmids. Want a retroviral plasmid with your gene of interest? Search our database for retroviral ...Puro IRES GFP empty plasmid) MSCV For cloning and gene expression; select with puromycin or screen for ...'s genome, which can lead to oncogenesis (termed insertional mutagenesis). Read our γ-Retrovirus Guide...Learn about retroviral plasmids at Addgene, including popular packaging, envelope, and transfer plasmids...virus uses the reverse transcriptase in tow to generate a DNA provirus. This provirus then gets integrated...to ∼8 kb), and infection results in long-term transgene expression. Some disadvantages of γ-retroviruses...

...zebrafish genes; many of these were generated as part of an NIH-sponsored effort to provide gene-editing...zebrafish genes. TALengineering... TALengineering Reagents TALEN Zebrafish Reagents TALENs for Endogenous Zebrafish Gene Targets...gene-editing nucleases to the zebrafish community. Target Gene Name TALENs Full Target Site (5' to 3'; half-sites...

...possible deletion strategies for genes and non-coding elements. For creating a gene knockout, two sgRNA located...evaluate the RNA, perform RT-qPCR for gene expression of the relevant gene 7,8 . To evaluate the protein, perform...genome editing tool that allows genetic perturbation of genes and genetic elements. Here we present a simple...for efficient loss-of-function studies of genes and genetic elements in mammalian cell lines. Protocol...theory and methods, perspectives for drug delivery, gene therapy and research. Gehl J. Acta Physiologica...Cell Lines Other Addgene Resources CRISPR Plasmids CRISPR Guide Addgene Blog Posts Addgene is proud to present... be utilized, such as pX458 (Addgene plasmid ID 48138) or pX459 (Addgene plasmid ID 48139), which include...

...deposit@addgene.org to be paired with an Addgene scientist. MAPK Plasmids (organized by gene) Gene Alternate... numerous research labs from around the globe, Addgene has assembled a MAPK plasmid collection for community...out our Suggest a Plasmid form or e-mail help@addgene.org to help us improve this resource!...

...transcription factor collection that contains 1,836 genes encoded by 3,548 isoforms and two control vectors...10X scRNA-seq (PDF, 59.2 KB) Plasmids ID Plasmid Gene/Insert Pooled Library The MORF pooled library is... transcription factor collection, available at Addgene. The collection can be used for screening transcription... entire pooled collection, can be purchased at Addgene. Each transcription factor isoform has a unique...out our Suggest a Plasmid form or e-mail help@addgene.org to help us improve this resource!...

... ID Plasmid Gene/Insert Promoter Selectable Marker PI Publication Bacteria ID Plasmid Gene/Insert Selectable...Transcriptional Regulation Activate Interfere Epigenetics RNA Targeting RNA Targeting RNA Editing Other...out our Suggest a Plasmid form or e-mail help@addgene.org to help us improve this resource!...

...table based on plasmid name, species, gene name, and more. Plasmid Gene/Insert Vector Type PI Publication ...cell lines with endogenously-tagged gene variants or browse Addgene’s entire collection of Stem Cell Research...against p53 in different gene/insert combinations A more efficient method to generate integration-free human...non-integrating plasmid from blood cells with unique epigenetic and gene expression signatures. Cell Res. 2011 Mar...human cells to pluripotency by activating enogenous genes Human pluripotent reprogramming with CRISPR activators...S1934-5909(19)30423-0. Schöler You can also find gene-specific plasmids at the following links: NANOG ...Browse Addgene's collection of plasmids for the generation and study of stem cells, from iPSC generation...