... gRNAs By Species Mammalian Bacteria Drosophila Plant C. elegans Yeast Zebrafish Xenopus CRISPR Resources...target the dCas9-activator to your specific locus. Want more information on the wide variety of Cas enzymes...expression in mammalian systems, bacteria, Drosophila, plants, and yeast. Mammalian ID Plasmid Gene/Insert Promoter... ID Plasmid Gene/Insert Promoter PI Publication Plant ID Plasmid Gene/Insert Promoter Selectable Marker...

... gRNAs By Species Mammalian Bacteria Drosophila Plant C. elegans Yeast Zebrafish Xenopus CRISPR Resources...the collateral RNA degradation seen in bacteria. Want more information on the wide variety of Cas enzymes... expression in mammalian systems, bacteria, and plants. Mammalian ID Plasmid Gene/Insert Promoter Selectable...Bacterial ID Plasmid Gene/Insert PI Publication Plant ID Plasmid Gene/Insert PI Publication Do you have...

... gRNAs By Species Mammalian Bacteria Drosophila Plant C. elegans Yeast Zebrafish Xenopus CRISPR Resources...HDR occurs at a much lower efficiency than NHEJ. Want more information on the wide variety of Cas enzymes...expression in mammalian systems, bacteria, Drosophila, plants, C. elegans, yeast, zebrafish, and Xenopus. Mammalian...Insert Promoter Selectable Marker PI Publication Plant ID Plasmid Gene/Insert Promoter Selectable Marker...

... gRNAs By Species Mammalian Bacteria Drosophila Plant C. elegans Yeast Zebrafish Xenopus CRISPR Resources... increases off-target effect. Which species or variant of Cas9 ( S. pyogenes , S. aureus etc.) was this...compatible with a single species or PAM binding variant of Cas9. For instance, wild-type SpCas9 must be...information and assistance with the deposit process. Want more information on the wide variety of Cas enzymes...

...mild to severe respiratory disease in humans. Advantages of adenoviruses in research include their large...adenoviruses do not integrate into the host genome. Disadvantages of adenoviruses include transient transgene ...transgene expression, as well as the substantial immune response induced by the viral capsid. Adenoviruses can...Plasmids Common shuttle and adenoviral plasmids. Want an adenoviral plasmid with your gene of interest...

...protein, and is suitable for both cell-permeant and non-cell-permeant proteins. CLIP-tag CLIP-tag is identical...proteins with disulfide bridges such as antibodies. MCP-tag is a mutant of ACP-tag, and is labeled by SFP Synthase...

...Loss of the tumor suppressor p53 also promotes aberrant mTORC1 activity, and multiple familial cancers...uncontrolled cell growth and proliferation, including an abundant supply of macromolecules and energy, as well as... as the presence of both pro-proliferative and anti-apoptotic signals. mTORC2, which appears to signal... mTOR signaling in growth control and disease. Laplante M, Sabatini DM. Cell. 2012 Apr 13;149(2):274-93...

...single-stranded breaks ( nicks ). ID Name Insert Function Antibiotic Resistance Activity PI 52962 lentiCas9-Blast ... a transcriptional activator . ID Name Insert Antibiotic Resistance Activity PI 61422 dCAS9-VP64_GFP dCAS9... express fluorescent proteins. ID Name Insert Antibiotic Resistance Purpose PI 17446 pLenti CMV GFP Hygro... in a new window) . Please note this does not guarantee viral service, but lets us know what viruses would...

...for use with virus-mediated delivery has many advantages for research. Viruses facilitate the delivery...Stable RNA <8 Kb 80-130 Dividing Yes Moderate * Recombinant AAV has a low frequency of target cell genome... Lab Lentivector Toolbox Guide to Generating Recombinant Adenoviruses Eric Campeau's Viral Vector Guide...Biosafety Resource Guide . References Cohen S, Au S, Panté N. How viruses access the nucleus. Biochim Biophys...

...during Development and Regeneration - Periklis Pantazis Lab Multibow: digital spectral barcodes for cell... loss and ablation of previously resistant cell types. CreLite - George Eisenhoffer Lab. ...for Zebrafish Receptors and Zebrafish Monoclonal Antibody plasmids - Gavin Wright Lab. Collections of plasmids...that encode zebrafish receptors or monoclonal antibodies to zebrafish cell surface receptors and secreted...

...viral genome and the absence of unexpected DNA contaminants. The data is also used to determine recombination...properties of the final vector preparation, the quantity of gram-negative bacterial endotoxin is ensured...within representative vector preparations was quantified with electron microscopy after negative staining...Approximately 2 weeks post-thaw, cell culture supernatant is tested for mycoplasma contamination. To date...

...computational pipeline that enables accurate quantification and visualization of CRISPR-Cas9 outcomes, ...off-target sites. This algorithm allows for the quantification of both non-homologous end joining (NHEJ) and...experiment a reference amplicon sequence to assess and quantify the efficiency of the targeted mutagenesis The...mutations. Coding sequence/s may be provided to quantify frameshift and potential splice site mutations...

...tags, can be used for enChIP, as well as an anti-Cas9 antibody. Biotin tagging of dCas9 can be achieved ... gRNAs By Species Mammalian Bacteria Drosophila Plant C. elegans Yeast Zebrafish Xenopus CRISPR Resources...

...gene targets. To accomplish this they have taken advantage of the Zhang lab all-in-one (guide/Cas9/reporter... with EF1alpha addition of an mCherry reporter variant nuclear reporters via an H2B fusion shuttle plasmid...fusion for gene activation CRISPR/Cas9 Plasmid Variants Figure 1: Schematic of the CRISPR Collection. ...

...available as cgDNA, we hope to aid scientists who want to immediately start cloning upon arrival of their...determined by spectrophotometer. Other methods of quantification may yield slightly different DNA concentrations...Suggestion Form . Please note that this does not guarantee that Addgene will make cloning grade DNA preps...

... Medicines Initiative 2 Joint Undertaking under grant agreement No 777372. This Joint Undertaking receives... production is used for assays and to generate antibody/ high-affinity ligands. Data is embedded in an...knowledgebase that features, among others, genetic variants and disease associations. Plasmids This plasmid...

...-type malate dehydrogenase genes and watermelon mutant clones for your course-based undergraduate research...single protein with many available isoforms and mutants provides a shared experience for the research community...of wild-type MDH genes from several species and mutant clones from watermelon have been codon-optimized...

..., not enough protocols, cost, not enough good antibodies, access to reagents)? For All Attendees: To help...for this meeting's publication, we are asking participants to share practical information in the form of... practices (e.g., dose response data, list of antibodies and recommended dilutions, etc.), novel applications...

...See below for plasmid kits and other resources relevant to these organisms, or use our Search page to ...Creating three-part fusion proteins - Planas and Santalucia Labs pCri System : Heterologous protein expression...MoClo : Modular cloning for applications like recombinant protein purification and cell-free circuit design...

...though actual yields may vary by construct. Size Quantity Total Volume Minimum Titer Typical Titer Small... on Request preps is all-inclusive of several important steps: MTA facilitation for the transfer plasmid...you need more assistance with troubleshooting, we want to hear from you! Email us at [email protected] ....