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  1. Qi Lab CRISPR Page

    Type
    Collection
    ...binding platform, to repress expression of arbitrary genes in bacteria or human cells ( Qi et al. ). This CRISPR...juxtaposed to the DNA complementary region. The uniqueness of CRISPRi, as compared to several recently published...applying the wild-type CRISPR system for genome mutagenesis ( Cong et al. , Mali et al. , Jiang et al. , ... could cause up to 300-fold repress on targeted genes. 2. Two-plasmid CRISPRi system for mammalian gene... up to 2~3-fold repress on targeted fluorescent genes. These plasmids are described in: Repurposing CRISPR...

  2. Plasmids for Stem Cell Research

    Type
    Collection
    ...Collection for plasmids that can be used to create cell lines with endogenously-tagged gene variants or browse...human cells to pluripotency by activating enogenous genes Human pluripotent reprogramming with CRISPR activators...lentiviral plasmids Induced Pluripotent Stem Cell Lines Derived from Human Somatic Cells. Science. 2007 ...stem cells. Stem Cells. 2009 May . 27(5):1042-9. Townes Lentivirus Human Single polycistronic lentiviral...from four separate plasmids Defining molecular cornerstones during fibroblast to iPS cell reprogramming ...iPSCs Bipolar Neurons Lentiviral Human Rapid neurogenesis through transcriptional activation in human ...

  3. CRISPR Plasmids - Tagging

    Type
    Collection
    ... induced pluripotent stem cell (hiPSC) lines. These cell lines were created using CRISPR and the donor...tagging transcription factors with FLAG in human cell lines. To repeat the tagging in your system, use the ...terminal affinity tag (3xFLAG-2xSTREP) on endogenous genes for the isolation of native protein complexes. This...be scaled up for systematic editing of multiple genes. Plasmids can be found associated with the following...

  4. Michael Davidson Fluorescent Protein Collection

    Type
    Collection
    ...Empty Backbones mEmeralds Reds Blues Cyans Oranges Greens Yellows Photoactivatables Empty Backbones Plasmid...lab fluorescent protein fusion constructs and backbones. Plasmid...Collection More Fluorescent Protein Resources: Empty Backbones FRET Subcellular localization Optogenetics The...

  5. Plan Your Experiment

    Type
    Collection
    ...more effective than dCas9 alone for mammalian cell lines. Activate (CRISPRa) Increase expression of an endogenous...cells, or selection marker to generate stable cell lines (for details, see Plasmids 101: Mammalian Vectors...and/or gRNA in a wide variety of mammalian cell lines Useful for difficult to transfect cell types, can...Protocol for genomic deletions in mammalian cell lines Select Your Target Sequence and Design Your gRNA...subcloning into a plasmid, and screening individual clones. PCR amplification and next-generation sequencing...

  6. Brzezinski Lab CRISPR Collection

    Type
    Collection
    ...modifications to the the pX458 plasmid to expand its usefulness for the lab's gene regulation and developmental...The plasmids listed here are provided as empty backbones that utilize Golden Gate cloning to insert the...E., Schneider, S. R., Abraham, J., Hensley, A., Jones, K. L., & Brzezinski, J. A. (2021). Initiation of...fate specification, yet differs from deleting both genes . Development. doi: 10.1242/dev.190272. PMID: 32541005...

  7. Malate Dehydrogenase CUREs Community Collection

    Type
    Collection
    ...of wild-type malate dehydrogenase genes and watermelon mutant clones for your course-based undergraduate...diverse set of wild-type MDH genes from several species and mutant clones from watermelon have been codon-optimized...

  8. Zinc Finger Consortium: Zinc Finger Arrays

    Type
    Collection
    ...targeted to various zebrafish genes. The table below lists zebrafish genes and associated pairs of zinc...A list of zebrafish genes and associated pairs of zinc finger arrays that can be used to generate ZFNs...):1471-1501. PubMed PMID 19798082 . Targeted mutagenesis in zebrafish using customized zinc-finger nucleases...

  9. Arf GTPase Family

    Type
    Collection
    ...GTPase Plasmids by Gene The following table lists genes in the ARF GTPase family, information on each gene...list of plasmids available. Please note that some genes may not yet have plasmids available for request.... GEF Plasmids by Gene The following table lists genes in the ARF GAP and GEF family, information on each...

  10. p53 Pathway

    Type
    Collection
    ...in promoting cell cycle arrest, apoptosis, or senescence in damaged cells. The p53 name arose from its...pro-apoptotic effects is especially important to tumorigenesis. Inheriting only one functional copy of the ... 1; also known as GTSE1 BAI-1 Brain-specific angiogenesis inhibitor 1 Bax BCL2-associated X protein Bid... phenotypes. Tyner SD, Venkatachalam S, Choi J, Jones S, Ghebranious N, Igelmann H, Lu X, Soron G, Cooper...

  11. TALEN Guide

    Type
    Collection
    ... effectors, scientists in several biological disciplines are coming closer to this DNA targeting ideal...di-residue (RVD). Ultimately, it is this RVD that determines which single nucleotide the TAL effector will... Daniel Voytas’s group from the University of Minnesota, arrays can be assembled in a matter of days. ...can be assembled and inserted into a variety of backbones in just a few steps ( Figure 2 ). The Bogdanove...

  12. Open Enzyme Collection

    Type
    Collection
    ... Depositor Collections FreeGenes Project Open Enzyme Collection FreeGenes Project Open Enzyme Collection...Open Enzyme Collection consists of more than 75 genes for enzymes that are considered "workhorses" of ...Additionally, the Open Enzyme collection contains enzyme genes for fixing damaged and degraded DNA prior to amplification...

  13. Fujii Lab CRISPR Plasmids

    Type
    Collection
    ...10.1186/s13104-018-3262-4. PubMed . Transgenic mouse lines expressing the 3xFLAG-dCas9 protein for enChIP analysis...Tanigawa N, Yuno M, Ikawa M, Taniuchi I, Fujii H. Genes Cells. 2018 Apr;23(4):318-325. doi: 10.1111/gtc.12573... Fujita T, Yuno M, Suzuki Y, Sugano S, Fujii H. Genes Cells. 2017 Jun;22(6):506-520. doi: 10.1111/gtc.12492... ribonucleoproteins. Fujita T, Yuno M, Fujii H. Genes Cells. 2016 Apr;21(4):370-7. doi: 10.1111/gtc.12341...

  14. Adeno-associated virus (AAV) Plasmids

    Type
    Collection
    ...AAVs can be handled at BSL-1, AAVs expressing oncogenes or toxins should be handled at BSL-2. AAV can ...plasmids (e.g., pHelper) and specific packaging cell lines (e.g., HEK293 cells) during AAV production. Read...encoding AAV Rep and respective capsid protein which determines the AAV serotype) are used in conjunction with...pRepCap6 AAV6 pRepCap6 contains both rep and cap genes from the AAV6 genome, but lacks the viral terminal...

  15. iPSC Neurodegenerative Disease Initiative Plasmid Collection

    Type
    Collection
    ...single nucleotide variant lines in an isogenic cellular background. All cell lines and phenotypic datasets...The following plasmids can be used to create cell lines with endogenously-tagged gene variants or to differentiate...

  16. KLF Research Plasmids

    Type
    Collection
    ...Michael Ruppert Michel Sadelain James Thomson Tim Townes Robert Weinberg Knut Woltjen Shinya Yamanaka Feng... metabolism, fibrosis, atherosclerosis, and carcinogenesis. The defining feature of the KLF family is ...Addgene or email [email protected] to get started. Genes KLF gene pages with information on available plasmids...

  18. Fluorescent Protein Guide

    Type
    Collection
    ...Protein Plasmids & Resources You May Also Like... Luminescence: Luciferase Plasmids Blog: Which FP Should I...Fluorescent Protein Plasmids by Category Empty Backbones Understand the localization and/or function of...fluorescent proteins, as well as over 100 empty backbones. Fluorescent Protein Kits cpFRET Kit: FRET based...

  19. CRISPR Plasmids - Parasites

    Type
    Collection
    ...(toxoplasmosis), Trypanosoma (African sleeping sickness), Cryptosporidium (cryptosporidiosis), Leishmania...have enabled precise genetic analyses to pinpoint genes linked to parasite virulence, transmission, and ...10 guides against 8,158 predicted protein-coding genes. Do you have suggestions for other plasmids that...

  20. Bikard Lab - CRISPR Repression Collection

    Type
    Collection
    ...simultaneously. By fusing sfGFP and/or mCherry to genes of interest, it is thus possible to study the effect...enables robust, noiseless knockdown of bacterial genes. Molecular Systems Biology, 14(3):e7899. doi: 10.15252... CRISPR knockdown to modulate expression of two genes without cross‐talk. (A) Schematic of the strain ...
Showing: 41 - 60 of 111 results