... Carrier Proteins (SLCs) from the RESOLUTE collection AAV Packaging from Addgene Parkinson's Research ...SARS-COV-2, ACE2, and CRISPR tools Fluorescent Proteins from the Michael Davidson collection Protein Labeling...available to industry scientists. ID Plasmid Gene/Insert PI Kits Kit name Type PI Description High Complexity...Screening Messer and MJFF Created for research in Parkinson's Disease; For target validation and drug design...agreement between the recipient and the depositing institution(s). It governs the transfer of biological materials...distribute plasmids on behalf of the depositing institutions. These agreements indicate the language which... by an authorized signatory at the recipient institution. An authorized signatory is someone who can execute...

...nickase. The fusion enzyme is capable of installing targeted insertions, deletions, and point mutations using...Plasmid Gene/Insert Promoter Selectable Marker PI Publication Bacteria ID Plasmid Gene/Insert Promoter Selectable...Plasmid Gene/Insert Promoter Selectable Marker PI Publication Drosophila ID Plasmid Gene/Insert Promoter ...edited 3’ flap is excised, the target sequence remains unchanged and available as a substrate for another...small prime editors with optimized RT and/or Cas9 domains PE7 - addition of RNA-binding exonuclease protection...enzyme, selectable marker, and whether the plasmid contains Cas9. ID Plasmid Expression System Promoter Guide...

...Includes many N- and C-terminal fluorescent proteins Insect Sutherland Lab Plasmids - For multicistronic...different fluorescent tags for you to create fusion proteins with your gene of interest. Special... Monomer not a Monomer? Background Fluorescent proteins are genetically encoded tools that are used extensively...have engineered numerous GFP-variants and non-GFP proteins that result in a diverse set of colors. Addgene...different fluorescent tags for you to create fusion proteins with your gene of interest. These are organized... at high concentration, so this is noted here. Proteins that are derived from GFP and contain the A206K...information on the properties of many of these proteins, see: Cranfill et al. , Nature Methods 2016 Jul...

...ID Plasmid Description Gene/Insert PI Plasmids Expressing Worm Genes/Inserts The table below lists plasmids... vectors for tag insertion using CRISPR/Cas9 and the selection of C. elegans strains. CRISPR plasmids ...includes CRISPR, RNAi, empty backbones, fluorescent proteins, and Cre expression plasmids. Large collections...briggsae , etc.) gene or sequence: ID Plasmid Gene/Insert PI Don’t see your gene listed? Know of some great...elegans genome engineering - Bob Goldstein Lab. Contains a set of plasmids for building homologous repair...Transgenesis Kit - Erik Jorgensen Lab. This kit contains 29 plasmids used to generate transgenic nematodes...Caenorhabditis Genetics Center (CGC) - The CGC collects, maintains, and distributes stocks of C. elegans. Silencing...

...into the host genome, it has the potential for insertional mutagensis. The 2nd and 3rd generation lentiviral...Type Description PI 12253 pRSV-Rev 3rd Packaging Contains Rev; can be used with 3rd generation transfer ...Rawlings , Scharenberg 12251 pMDLg/pRRE 3rd Packaging Contains Gag and Pol; can be used with 3rd generation transfer... encoding Rev Trono 12260 psPAX2 2nd Packaging Contains Gag, Pol, Rev, and Tat; can be used with 2nd and...plasmids Trono 12263 pCMV delta R8.2 2nd Packaging Contains Gag, Pol, Rev, and Tat; can be used with 2nd and...plasmid Weinberg 8455 pCMV-dR8.2 dvpr 2nd Packaging Contains Gag, Pol, Rev, Tat, Vif, and Nef; can be used ...envelope plasmid Kasparov 22500 pNHP 2nd Packaging Contains gag, pol, tat, rev Kasparov 1732 pCPRDEnv 2nd ...

... - Inducible expression, fusion proteins, and more Sandmeyer Lab Plasmids...Lehner Lab vectors - Tools for nematode transgenesis Insect / Baculovirus Polyhedrin pFastBac LIC and pFastBac... Vectors from the Gradia lab pFB-LIC-Bse - Insect expression vector from Gileadi lab... Epitope Tag or Fusion Protein Tags and fusion proteins are excellent tools for further understanding...easily identify your protein using an antibody against that epitope. This could allow you to conduct...favorite gene even if you do not have an antibody against it. Another common scenario is fusing your... for C elegans expression Other Fluorescent Proteins Localization See full Fluorescent ...

...Bertozzi Nicolas Buchler George Church Cynthia Collins James Collins Tom Ellis Drew Endy Tobias Erb Susan Golden...environmental sensing. Strains Browse Addgene's collection of engineered bacterial strains, including plasmid-strain...terminators, repressors, activators, and more. Also contains pre-assembled genetic circuits such as logic gates...Autoregulation and Yeast-Mammalian Circuit Transfer C. Collins Lab Quorum Sensing Ellis Lab GeneGuard Endy Lab...Addgene Tools and Resources CRISPR Fluorescent Proteins FREQ-Seq Kit FX Cloning Kit GreenGate Cloning ...ethical synthetic biology JBEI - Joint Bio-Energy Institute Synberc - Synthetic Biology Engineering Research...

...locally or globally. The MECP2 protein contains several functional domains, including: the N - t erminal D omain...or bacterial) Insert species (mouse or human) Mutation ID Plasmid Description Gene/Insert Mutations PI ...ranscriptional R epressor D omain (TRD) the TRD contains a Nuclear receptor Co-Repressor 1/Silencing Mediator...that can be differentiated into neurons Coriell Institute: Patient-specific lines (Link opens in a new window...Repositories (Link opens in a new window) Coriell Institute - cell line repository, including patient-derived...Additional Resources (Link opens in a new window) BrainSpan Atlas of the Developing Human Brain - includes... 55–67. PMID: 30536762 Shahbazian et al. 2002. Insight into Rett syndrome: MeCP2 levels display tissue...

... handled at BSL-1, AAVs expressing oncogenes or toxins should be handled at BSL-2. AAV can transduce both...AAV particles without the use of a helper virus. Instead, specific gene products can be provided by helper...rep gene and polycistronic AAV-1 cap gene in Sf9 insect cells Kotin 65214 pSR657 AAV2 Baculovirus-mediated...rep gene and polycistronic AAV-2 cap gene in Sf9 insect cells Kotin 65215 pSR646 AAV6 Baculovirus-mediated...rep gene and polycistronic AAV-6 cap gene in Sf9 insect cells Kotin 65216 pSR660 AAV8 Baculovirus-mediated...rep gene and polycistronic AAV-8 cap gene in Sf9 insect cells Kotin 92307 pAnc80L65AAP Anc80L65AAP AAV ...system Gradinaru 110770 pRepCap6 AAV6 pRepCap6 contains both rep and cap genes from the AAV6 genome, but...

...synthesis. Additionally, the Open Enzyme collection contains enzyme genes for fixing damaged and degraded DNA...sequences to remove unwanted parts, and DNA-binding proteins that can be fused with other enzymes to improve...Other Enzymes DNA Ligases Addgene ID Plasmid Gene/insert 165503 pOpen-HSlig4 DNA Ligase IV 165514 pOpen-... to top DNA Polymerases Addgene ID Plasmid Gene/insert 165500 pOpen-9N7polA 9 degrees N-7 DNA polymerase...Restriction Endonucleases Addgene ID Plasmid Gene/insert 165504 pOpen-EcoRIR EcoRI 165506 pOpen-Eco31I Eco31I... Reverse Transcriptases Addgene ID Plasmid Gene/insert 165546 pOpen-MMLV_RT (mut H) Moloney Murine Leukemia... to top RNA Polymerases Addgene ID Plasmid Gene/insert 165505 pOpen-T7gene1 T7 RNA Polymerase 165516 pOpen-SP6rpol...

... Collections MJFF Parkinson's Plasmid Resource Michael J. Fox Foundation Parkinson's Disease Plasmid Resource..., and other proteins of interest. Browse or search the table below. ID Plasmid Gene/Insert Mutations Industry...Browse plasmids expressing genes relevant to Parkinson's disease created by the Michael J. Fox Foundation... researchers to assemble and share tools for Parkinson's Disease research. This collection is part of ...search the table below. ID Plasmid Description Gene/Insert Industry PI Return to top...

...of the viral capsid proteins are analyzed. The abundance of viral capsid proteins as a fraction of total...relative stoichiometric ratios of the viral capsid proteins VP1, VP2 and VP3. Samples of viral preparations...subjected to gel electrophoresis. Viral capsid proteins VP1, VP2, and VP3 are shown relative to the total.... Three to five days later, cell cultures are inspected for sterility. Transducibility Some viral vectors...virus from Addgene, read our detailed recipient instructions for next steps. Plan an experiment using our...to amplify a portion of the intended lentiviral insert. During this cultivation, cells are routinely checked...

... almost unidirectional insertions, but are more prone to off-target insertions. CASTs are most often used...Plasmid Gene/Insert Promoter Selectable Marker PI Publication Bacteria ID Plasmid Gene/Insert Selectable...for CRISPR-associated transposases (CASTs) for insertion of donor DNA. CRISPR...locations. The Cas effector lacks nuclease activity, instead recruiting the transposase (comprised of multiple...TniQ). Type I CASTs can result in bidirectional insertions and may not be suitable for applications where...

...developed class of tools are Luminopsins (LMOs), or luminescent opsins: fusion proteins of a light-emitting luciferase...specific biological processes. Addgene’s collection contains two main classes of chemogenetic receptors: Designer...muscarinic receptors and couple to the Gi, Gq, or Gs G-proteins, respectively. KORD is derived from the κ-opoid...engineered α7 nicotinic acetylcholine receptor (nAChR) domains that respond specifically to small molecules termed...chemogenetics plasmids. ID Plasmid Description Gene/Insert Vector Type Promoter Tags PI Do you have suggestions...

...fluorescent markers and tag your proteins of interest with fluorescent proteins in yeast....create fusions of proteins of interest with yeast codon-optimized fluorescent proteins. These vectors are...collection of yeast codon-optimized fluorescent proteins (FPs) in a variety of plasmids allows for constitutive...promoters and the KanMX marker. ID Plasmid Gene/Insert Selectable Marker Publication Additional Resources...Teusink B. In vivo characterisation of fluorescent proteins in budding yeast. Sci Rep. 2019 Feb 19;9(1):2234...

...Negative control vector for pMKO.1 puro; Contains shRNA against GFP William Hahn 10676 pMKO.1 GFP Derivative...expression; Recommended for cloning new shRNAs (contains a 1.9 kb stuffer region to easily visualize products... Negative control vector containing non-hairpin insert David Root 1864 pLKO.1 ‐ scrambled shRNA Negative... (e.g. pSUPER) can be cloned into this vector; Contains a 5'LTR and should be packaged using the 2nd generation...Derivative of pMKO.1 puro with GFP instead of puromycin resistance gene William Hahn Additional Resources...cells and transgenic mice by RNA interference. Rubinson DA, Dillon CP, Kwiatkowski AV, Sievers C, Yang...

...for genome editing, empty backbones, fluorescent proteins, reporters, biosensors, and Cre expression plasmids...are described below . ID Plasmid Description Gene/Insert PI Return to top Highlights of Zebrafish Tools ...somatic mutations, or labeling with fluorescent proteins to visualize and track clones of cells over time... lab. Targeting cassettes encoding fluorescent proteins for tagging genes at endogenous genomic loci in... zebrafish cell surface receptors and secreted proteins. Return to top Other Resources Addgene Blog Posts...Center (ZIRC) - A US-based repository for zebrafish strains and materials and information about zebrafish research...Center (EZRC) - A repository at the Karlsruhe Institute of Technology (KIT) for the distribution of zebrafish...

...methyltransferase INSR Insulin receptor INSRR Insulin receptor-related receptor IRS IRS1 IRS2 Insulin receptor ...for the gene, or browse the gene list below. For proteins with multiple subunits or isoforms, individual.... The RAS Initiative - at the National Cancer Institute (NCI) References Ras oncogenes and their downstream...

...Plasmid Gene/Insert Promoter Selectable Marker PI Publication Bacteria ID Plasmid Gene/Insert Promoter PI...Drosophila ID Plasmid Gene/Insert Promoter PI Publication Plant ID Plasmid Gene/Insert Promoter Selectable Marker...elegans ID Plasmid Gene/Insert Promoter PI Publication Yeast ID Plasmid Gene/Insert Promoter Selectable Marker...

...Plasmid Gene/Insert Promoter Selectable Marker PI Publication Bacteria ID Plasmid Gene/Insert Promoter Selectable...Plasmid Gene/Insert Promoter Selectable Marker PI Publication Plant ID Plasmid Gene/Insert Promoter Selectable...introduce gRNA-targeted single-strand breaks in DNA instead of the double-strand breaks created by wild type...PI Publication Yeast ID Plasmid Description Gene/Insert Promoter Selectable Marker PI Publication Do you...