...electroporation Transient expression of CRISPR components Expression decreases as RNA is degraded within the...multi-plasmid SAM system. Select Expression System To use CRISPR, you will need both Cas9 and a gRNA expressed...details, see Plasmids 101: Mammalian Vectors ) Transient or stable expression of Cas9 and/or gRNA in a mammalian...lentivirus, see our Lentivirus Guide ) Stable, tunable expression of Cas9 and/or gRNA in a wide variety of mammalian...Additionally, many plasmids containing validated gRNAs are now available through Addgene. These plasmids contain ...lack of gRNA and/or Cas9 expression or (2) a lack of efficient target cleavage in cells expressing both ... Science Guides CRISPR Guide Plan Your Experiment Plan Your CRISPR Experiment Jump To: Plan Your Experiment...

...Regulation Activate Interfere Epigenetics RNA Targeting RNA Targeting RNA Editing Other Applications Purify Tag...using any of these gRNA plasmids and review the publication associated with each plasmid for more information...information on the experimental use of these plasmids. ID Plasmid Gene/Insert Vector Type PI Publication Do...suggestions for other plasmids that should be added to this list? Fill out our Suggest a Plasmid form or e-mail... CRISPR gRNAs CRISPR Plasmids: gRNAs Browse CRISPR Plasmids...CRISPR Guide Viral Preps Protocols gRNA Design Tools CRISPR Blog Posts The table below lists gRNA plasmids...gRNA expression vectors available from Addgene. CRISPR...

...Fragmented T7 RNAP Plasmids Repressor Plasmids Sigma (σ) Factor Plasmids Terminator Plasmids Viral Vectors...Trimmer Lab Recombinant mAb Plasmid Collection CRISPR CRISPR-on: an RNA-guided transcriptional activator...visualization, protein expression, synthetic biology, and viral vector plasmids. Plasmid...Depositor Plasmid Collections Depositor Collections Addgene's repository contains 156,627 plasmids, which ... you find it helpful! You May Also Like: Plasmid Kits Plasmid Special Collections Pooled Libraries Antibodies...Collection Rett Syndrome Plasmid Collection and Resource Center TREAT-AD Plasmid Collection Visualization...Institute for Cell Science Plasmid Collection New England BioLabs Cell-Imaging Plasmid Collection Botman-Teusink...

...Activate Interfere Epigenetics RNA Targeting RNA Targeting RNA Editing Other Applications Purify Tag Visualize...below for CRISPR plasmids designed to introduce a DSB. Plasmids are available for expression in mammalian ...Mammalian ID Plasmid Gene/Insert Promoter Selectable Marker PI Publication Bacteria ID Plasmid Gene/Insert...Drosophila ID Plasmid Gene/Insert Promoter Selectable Marker PI Publication Plant ID Plasmid Gene/Insert... elegans ID Plasmid Gene/Insert Promoter Selectable Marker PI Publication Yeast ID Plasmid Gene/Insert...Zebrafish ID Plasmid Gene/Insert Promoter Selectable Marker PI Publication Xenopus ID Plasmid Gene/Insert...suggestions for other plasmids that should be added to this list? Fill out our Suggest a Plasmid form or e-mail...

...ways. Cas9 proteins can be guided to specific genomic sites by an RNA guide (gRNA) that harbors targeting... a list of gRNA plasmids targeting specific genes. For more information on CRISPR plasmids and function...are made from plasmids that are currently in our repository. You can obtain the plasmid from us or receive...biological processes. For more information, see our Guide to Plasmid Pooled Libraries . ID Name Description Genes... of available viruses based on plasmids in our repository that express fluorescent proteins. ID Name Insert...function, see our CRISPR plasmids resource page . Cas9 for Editing Genomic Sequences To perform genome edits...52962 lentiCas9-Blast Sp Cas9 Cut Blasticidin Expresses human codon-optimized S. pyogenes Cas9 protein...

... on the plasmid. gRNA plasmids that do not co-express a Cas protein require a separate plasmid that does...constructing all-in-one expression vectors containing multiple guide RNA expression cassettes and a Cas9 ...for sgRNA expression. Chen pCFD4-U6:1_U6:3tandemgRNAs Drosophila Single plasmid for the expression of ...Vectors CRISPR Plasmids: Empty gRNA Expression Vectors Browse CRISPR Plasmids By Function Genome Editing Cut...can be used to express multiple gRNAs from a single plasmid with or without expression of Cas9. Highlighted.... Can express Cas9 with up to 3 gRNAs. Frew pSQT1313 Mammalian Single plasmid for the expression of multipe...Regulation Activate Interfere Epigenetics RNA Targeting RNA Targeting RNA Editing Other Applications Purify Tag...

... Biosensors Luciferase Plasmids Subcellular Localization Optogenetics Plasmids Viral Service: AAV Biosensors... Ting Lab GCaMP6 Plasmids 2014 Jonathan Ting Calcium Intersectional viral expression of GCaMP6m/s in cells... lentiviral plasmids for a variety of fluorescent sensors to monitor cell physiology. RNA-based aptamer...suggestions for other plasmids that should be added to this list? Fill out our Suggest a Plasmid form or e-mail...S-adenosyl methionine . Reporters of miRNAs Toehold sensors for various target RNAs Explore the Fluorescent Biosensor...Collection of plasmids encoding fluorescent biosensors to measure biomolecules or genes via FRET or other... Plasmid Collections Fluorescent Proteins Fluorescent Biosensors Fluorescent Proteins: Biosensors...

... Jaenisch labs Plasmids MECP2 Plasmids The table below highlights plasmids that express or target the ...resources for a full selection of plasmids expressing Cas9 and empty gRNA backbones. A few examples of additional... editing, MECP2 reactivation, RNA editing, gene replacement therapy, RNA trans-splicing, and protein replacement...approximately half of cells expressing wild-type MECP2 and half expressing mutant MECP2 . Skewing of these...information on generating iPSCs using plasmids, please see Addgene's Plasmids for Stem Cell Research page. CRISPR... search bar or sort buttons to find plasmids based on: Expression system (mammalian or bacterial) Insert...human) Mutation ID Plasmid Description Gene/Insert Mutations PI Return to top External Resources Research...

... Science Guides Antibody Guide Antibody Guide View Addgene's Antibody Collection...hybridoma cells into plasmids. Plasmids are then transfected into cells and induced to express antibodies. Antibodies.... Recombinant expression validation A recombinant expression strategy is used to express the protein of...little variation. Recombinant plasmids - Antibodies can be produced from plasmids, with an antibody’s genetic...produce the same antibody clone, or from plasmid-based expression. Monoclonal antibodies are necessary in... comparison of expression between different samples, by normalizing protein expression to the controls...In our Antibody Guide, we define key terms, summarize antibody production and storage techniques, and...

...pBad-EBFP2 - Bacterial Expression EBFP2-N1 - Mammalian Expression EBFP2-C1 - Mammalian Expression EBFP2-pBAD - ...- Mammalian Expression pCyPet-C1 - Mammalian Expression pCyPet-His - Bacterial Expression CYPet-N1 - Mammalian...Bacterial Expression iLOV-N1 - Mammalian Expression iLOV-C1 - Mammalian Expression AcGFP1 475 505 28 Prone ... - Mammalian Expression sfGFP-C1 - Mammalian Expression sfGFP-pBAD - Bacterial Expression moxGFP 486 510...Mammalian Expression pCAG-GFP - Mammalian Expression pLV-eGFP - Mammalian Lentiviral Expression Ac5-STABLE1...1 - Mammalian Expression mEGFP-N1 - Mammalian Expression mEGFP-C1 - Mammalian Expression mEGFP-pBAD - ... - Mammalian Expression Topaz-C1 - Mammalian Expression Topaz-pBAD - Bacterial Expression mTopaz 514 527...

... Plasmid Collections Microbiology Plasmids Microbiology Plasmids and Resources Addgene...Resources Plasmid Kits for Microbiology : Sets of plasmids for fluorescence, cloning and expression, and synthetic...resources, or read our guide on assembly standards. Browse Plasmids by Microbe System Plasmids for Archaea Species...Biology Assembly Standards Guide Bacterial Expression Systems : Find plasmids to perform your experiment...bacterial expression system. Fluorescent Protein Guide : Addgene's extensive fluorescent protein plasmid collection...Vibrio sp. Yersinia pestis Plasmids for Cyanobacteria Cyanobacteria Plasmids for Fungi Species Aspergillus...Aspergillus sp. Cryptococcus sp. Plasmids for Prions Prions Plasmids for Protozoa Species Entamoeba histolytica...

...nucleocapsid protein (Brian Geiss). Plasmids SARS-CoV-2 Plasmids Many plasmids containing SARS-CoV-2 sequences...distribute SARS-CoV-2 plasmids. The collection contains: Plasmids for E. coli and Yeast expression Spike protein...cytokines and related plasmids see our Immunology Resource Guide . Return to top General Plasmid Tools SHERLOCK...a new window) BST-LF expression plasmid from Andrew Ellington's lab. Find plasmid tools that may be helpful...Addgene will maintain this plasmid collection page, which highlights plasmids available that may be useful...SARS-CoV-2 nucleocapsid protein. SARS-CoV-2 Plasmids : Plasmids that are available or coming soon containing...containing SARS-CoV-2 sequences. SARS-CoV Plasmids : Plasmids that are available containing SARS-CoV (SARS ...

... may also like... Lentiviral Guide Adenoviral Guide Viral Vector Plasmids and Resources Addgene Blog: ...: Your Lentiviral Plasmid FAQs Biosafety Resource Guide Lentiviral Transfer Vectors Adenoviral Vectors...reporter plasmids to test whether or not you've efficiently generated infectious virus, plasmids expressing...-EF1-DIO-copGFP 72253 Expresses copGFP under EF-1 promoter when Cre is expressed by another vector pCDH-EF1...pCDH-EF1-DIO-tdTomato 72254 Expresses tdTomato under EF-1 promoter when Cre is expressed by another vector pCDH-CB-iCre-P2A-tdTomato-T2A-Puro...pCDH-CB-copGFP-T2A-iCre 72256 Expresses copGFP and iCre under from the CB promoter pCDH-CB-iCre 72257 Express iCre under ...pCDH-CB-FLPe-P2A-tdTomato 72259 Expresses FLPe and tdTomato from the CB promoter pCDH-EF1-Fon-copGFP 72260 Expresses copGFP from...

...Species-Specific Expression If you want to drive expression of your favorite gene, you will need a plasmid with... expression - Gradia Lab plasmids for mammalian expression, Ligation Independent... plasmids Fly UAS, MT Targeted Gene Expression - Rubin lab plasmids for drosophila...collection of plasmids for packaging and expression. See our dedicated lentiviral plasmid page . ...Tet-inducible lentiviral shRNA expression pMKO.1 puro - Retroviral shRNA expression pLenti CMV Puro... shRNA expression pSico PGK GFP - Conditional shRNA expression through lentiviral...genetic element or want to express shRNA. Choose by: Species-specific expression Epitope tag or fusion protein...

... Plasmid Collections Worm Plasmids Worm Plasmids and Resources Plasmid Tools Worm Constructs...some of the plasmids and tools available at Addgene for use in worms. Browse Plasmids Plasmid Tools for ... Gene/Insert PI Plasmids Expressing Worm Genes/Inserts The table below lists plasmids that contain a worm...'s worm CRISPR resources include plasmids expressing Cas9, empty gRNA backbones, and constructs for endogenous...backbones, fluorescent proteins, and Cre expression plasmids. Large collections or kits are described below...complete list of plasmids that can be used in worms, search Addgene's entire collection. ID Plasmid Description...sequence: ID Plasmid Gene/Insert PI Don’t see your gene listed? Know of some great plasmid tools for the...

...lentiviral plasmids to express your CRISPR gRNA? Please see our CRISPR Empty gRNA plasmids table and search... Viral Plasmids Lentiviral Plasmids Lentiviral Plasmids Additional Resources... Pooled Libraries All Viral Plasmids Packaging Plasmids Transfer Plasmids Lentiviruses, a subset of retroviruses...among multiple plasmids. Some plasmids (typically called packaging and envelope plasmids) encode components...generation transfer plasmids Trono 12259 pMD2.G N/A Envelope VSV-G-expressing envelope plasmid Trono 8454 pCMV-VSV-G...envelope plasmid Nienhuis and Salmon 35616 pCAG-VSVG N/A Envelope VSV-G-expressing envelope plasmid Nienhuis...transgene and CMV drives expression of RFP as a reporter. See plasmid 17618 for GFP plasmid. Cheng 17452 pLenti...

...List of plasmid kits and accessory plasmids available from Addgene for generating your own specific TALE...Genome Engineering Guide CRISPR Plasmids Zinc Finger Kits TALENs are important new tools for genome engineering...Yamamoto Additional Plasmids for Use with Addgene TALEN Kits For Use With: ID Plasmid Depositing PI Description...-inducible expression. pTAL6-BB contains the TEF1 promoter, giving constitutive expression of TALORs built... For both plasmids sequence positions 1214–2210 of pTAL3 were cloned into a pCS2 expression vector resulting...Maria-Elena Torres-Padilla These plasmids were designed for mammalian expression of fluorescent TAL effectors...pTAL4. The pTAL7 destination plasmids were optimized for TALEN expression in mammalian systems including...

...Homepage Plasmid Collections Addgene Plasmid Collections Addgene's repository contains 156,627 plasmids . We've...resources group plasmids together based on type or application. Browse our guides to find the plasmids and science... EXtracellular Plasmid RESource (EXPRESs) Consortium Krüppel-like factors (KLF) Plasmid Resource RESOLUTE...Mammalian RNAi Microbiology Plant Expression Stem Cells Synthetic Biology Tet Inducible Expression Worm Expression...you are looking for a non-plasmid item, browse our entire repository . Plasmid Resources Our curated resources...Viral Plasmids Lentivirus Adeno-associated Virus (AAV) γ-Retrovirus Adenovirus All Viral Plasmids Curated...Curated Topics Antibody Plasmid Collection Bacterial Expression Systems Empty Backbones Genetic Code Expansion...

...SepOTSγ Plasmid 68285 SepOTSδ Plasmid 68286 SepOTSϵ Plasmid 68287 SepOTSζ Plasmid 68288 SepOTSη Plasmid...SepOTSθ Plasmid 68290 SepOTSι Plasmid 68291 SepOTSκ Plasmid 52054 SepOTSµ ( aka. B40 OTS) Plasmid 68294 ...68294 SepOTSν Plasmid 68295 GFP E17TAG Plasmid 68296 GFP S2TAG Plasmid 68297 GFP Q157TAG Plasmid 68298 GFP...Amp) Plasmid 68300 MBP-MEK1 Plasmid 68301 MBP-MEK1 S218TAG Plasmid 68302 MBP-MEK1 S222TAG Plasmid 68305...pKD-SepRS-EFSep) Plasmid 34624 SepOTSα tRNA-Sep ( aka. pCAT112TAG-SepT) Plasmid 68283 SepOTSβ Plasmid 68284 SepOTSγ... 68306 C321.ΔA.Δserb.Amp S Plasmid 68292 SepOTSλ Plasmid 68307 SupD Plasmid 34623 SepOTSα SepRS/EF-Sep...( 188537 ) is a ROP minus plasmid and is not compatible with other plasmids containing high copy ColE1...

...Receptor Plasmids Find Plasmids by Gene Name Alternative Gene Names Gene Description AR plasmids AR, RP11... Plasmid Collections Nuclear Receptor Plasmid Collection Nuclear Receptor Plasmid Collection... Nuclear Receptor plasmid collection for the community. If you have created plasmids that you would like...ESRRB Plasmids ESRRB, DFNB35, ERR2, ERRb, ESRL2, NR3B2 estrogen-related receptor beta ESRRG Plasmids ESRRG...group A, member 1 PGR Plasmids PGR, NR3C3, PR progesterone receptor PPARA Plasmids PPARA, SC22CB-5E3.5,...receptor gamma RARA Plasmids RARA, NR1B1, RAR retinoic acid receptor, alpha RORA Plasmids RORA, ROR1, ROR2...orphan receptor C RXRA Plasmids RXRA, NR2B1 retinoid X receptor, alpha RXRG Plasmids RXRG, NR2B3, RXRC retinoid...