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Showing: 41 - 60 of 108 results

  1. CRISPR References and Information

    Type
    Collection
    ...silkworm, and budding and fission yeast. Target Finder (Feng Zhang lab) Identifies gRNA target sequences from... non-model invertebrate species. Forums and FAQs Feng Zhang lab: Tips for CRISPR design, homology directed... cloning . CRISPR Discussion Group (moderated by Feng Zhang lab): Forum/FAQ Drosophila: General Advice...

  2. TALENs for Endogenous Zebrafish Genes

    Type
    Collection
    ... TALengineering Reagents TALEN Zebrafish Reagents TALENs for...Zebrafish Gene Targets Recent reports have shown that engineered TALENs can be used to induce targeted mutations...table below lists pairs of TALENs that have been engineered by the Joung, Peterson, and Yeh labs at MGH to...

  3. Optogenetics AAV Preps

    Type
    Collection
    ... GFP Red-wavelength fluorescent protein Yellow-wavelength fluorescent protein Blue-wavelength fluorescent...

  4. Immunology Research Plasmids and Resources

    Type
    Collection
    ...oncogene homolog, avian) ERBB, ERBB1, HER1, PIG61, mENA ENG endoglin CD105, END, FLJ41744, HHT1, ORW, ORW1 EPGN...receptor (TCR) engages with antigen presenting cells of the innate immune system. Engagement of the TCR by...antigens through the B cell receptor (BCR). The strength and duration of signaling controls the fate of...tolerance or anergy are induced when the TCR is engaged without co-stimulation or with a low affinity antigen...

  5. Bacterial Expression Systems

    Type
    Collection
    ... Systems Bacterial Expression Systems Genome Engineering Visualization and Tagging Purification Controlled... expression systems to find out more! Genome Engineering Although overexpressing or knocking down a gene...purified protein. Protein purification can be challenging, but once you get it right, you can learn many...

  6. KLF Research Plasmids

    Type
    Collection
    ...Research KLF Depositing Labs Juan Belmonte Linzhao Cheng Jose Cibelli George Daley Jin-Tang Dong Steven Dowdy...Townes Robert Weinberg Knut Woltjen Shinya Yamanaka Feng Zhang About The Krüppel-like factors (KLFs) are ...

  7. TALEN Expression Vectors

    Type
    Collection
    ... TALengineering Reagents TALEN Expression Vectors TALEN Expression... FLASH assembly methods can be found on the TALengineering website . All three of these methods yield ...

  9. Gersbach Lab CRISPR Plasmids

    Type
    Collection
    ...reagents for CRISPR/Cas9-based multiplex genome engineering in diverse cell types. This set of plasmids is...described in: Multiplex CRISPR/Cas9-based genome engineering from a single lentiviral vector. Kabadi AM, Ousterout...

  10. CRISPR Plasmids and Resources

    Type
    Collection
    ...immune system to a revolutionary tool for genome engineering. CRISPR Topic Page : Want the latest news on ...experiments. Browse CRISPR Plasmids by Function Genome Engineering Cut Wild-type Cas enzymes generate double strand...

  11. Microbiology Resources

    Type
    Collection
    ...gondii Trypanosoma sp. Plasmids for Viruses Species Dengue Hepatitis Influenza Mumps virus Rubeola (measles...Read our CRISPR Methods for Bacterial Genome Engineering blog post to see how Addgene depositors are making...

  12. Open Enzyme Collection

    Type
    Collection
    ...functionality or add new functions through genetic engineering and directed evolution. More information about...polymerase 165543 pOpen-Bstpol Bst DNA Polymerase, Full Length 165544 pOpen-T5gene122 T5 DNA Polymerase 165553...

  13. Zebrafish Plasmid Collection

    Type
    Collection
    ...and non-coding RNAs in zebrafish - Tatjana Sauka-Spengler Lab Lineage Tracing Cell lineage tracing in multicellular...drivers in vivo. Biotagging toolkit - Tatjana Sauka-Spengler Lab. A genetic binary system for cell- and compartment-specific...

  15. COVID-19 Resources

    Type
    Collection
    ...Finding nucleic acids with SHERLOCK and DETECTR . Feng Zhang's lab at the Broad Institute has utilized ...Center The Lancet COVID-19 Resource Centre The New England Journal of Medicine Coronavirus webpage PLOS Journals...

  16. Genomic Deletions in Mammalian Cell Lines

    Type
    Collection
    ...re-purposed for site-specific eukaryotic genome engineering. CRISPR/Cas9 is an inexpensive, facile, and efficient...manuscript here: Bauer et al, 2014 322.2 KB Genome engineering using the CRISPR-Cas9 system. Ran FA...

  17. Plasmids for Stem Cell Research

    Type
    Collection
    ...Cells Transl Med. 2015 Mar 5. pii: sctm.2014-0214. Cheng RNA Human Reprogramming factors for delivering synthetic...expression signatures. Cell Res. 2011 Mar;21(3):518-29. Cheng piggyBac Mouse Doxycycline-inducible piggyBac transposon...

  18. Genetic Code Expansion

    Type
    Collection
    ...Because there are no free codons, this can be challenging. In E.coli , the rarest codon is the amber stop...the ncAA in the media to make sure that the full length protein is only made when the ncAA is included....

  19. Fluorescent Protein Guide: Subcellular Localization

    Type
    Collection
    ...fluorescent protein that has a different emission wavelength. For example, if the marker protein is fused ...Patricia Wadsworth 27123 eTC GFP beta-actin full length Actin Filaments beta-actin EGFP Robert Singer 27382...

  20. Fluorescent Protein Guide: Empty Backbones

    Type
    Collection
    ... Gene, 1992 ), and since then scientists have engineered numerous GFP-variants and non-GFP proteins that...Photoactivatable (e.g. off to on) Excitation and Emission wavelengths after activation. Protein Excitation (nm) Emission...
Showing: 41 - 60 of 108 results