Skip to main content
Addgene
Showing: 41 - 50 of 50 results

  1. Genomic Deletions in Mammalian Cell Lines

    Type
    Collection
    ...the usage of the pSpCas9(BB) plasmid (pX330) (Addgene plasmid ID 42230). This plasmid allows for the simultaneous...Mammalian Cell Lines Other Addgene Resources CRISPR Plasmids CRISPR Guide Addgene Blog Posts Addgene is proud.... Perform maxi-prep for each CRISPR/Cas9 construct. Transfecting CRISPRs into Cells of Interest NOTE: ...: This protocol involves the delivery of CRISPR/Cas9 plasmids by electroporation 4 . This protocol is ...cells. However, transient transfection of CRISPR/Cas9 plasmids may be successfully adapted to numerous...using CRISPR/Cas9 CRISPR... CRISPR References & Information Genomic Deletions in Mammalian Cell Lines CRISPR: Protocol...

  2. CRISPR Plasmids - Epigenetics

    Type
    Collection
    ... CRISPR Epigenetics CRISPR Plasmids: Epigenetics Browse CRISPR Plasmids By Function Genome...Zebrafish Xenopus CRISPR Resources CRISPR Guide Viral Preps Protocols gRNA Design Tools CRISPR Blog Posts To...Purify Tag Visualize dCas9-FokI Screen Pooled Libraries gRNAs Empty gRNA Vectors gRNAs By Species Mammalian...CRISPR plasmids for targeted epigenetic modification, including histone acetylation/demethylation, and...Browse, sort, or search the tables below for CRISPR plasmids for epigenetic modification in mammalian or...Mammalian ID Plasmid Gene/Insert Promoter Selectable Marker PI Publication Plant ID Plasmid Gene/Insert...suggestions for other plasmids that should be added to this list? Fill out our Suggest a Plasmid form or e-mail...

  3. Neurodegeneration Research Collection

    Type
    Collection
    ... and plasmid collections that may help with your neurodegeneration research. Disease Info Plasmid Collection...Research Tools Neurodegeneration Plasmid Collection A collection of plasmids expressing wild type or mutant...gene fragments, and gRNAs for genes linked to neurodegenerative disorders. Find plasmids expressing HTT, ...Dhekne et al. Elife. 2023 Oct 24. See More CRISPR Tools Find CRISPR pooled libraries available from Addgene...Target neural oxytocin receptors using an AAV-CRISPR/Cas9 strategy for gene editing across divergent rodent...Subcellular Localization, Optogenetics Plasmids, and Chemogenetics Plasmids. New and Noteworthy: Explore NeuroPAL...more. Plasmids provided by the foundation can be found on our Michael J. Fox Foundation Plasmid Resource...

  4. CRISPR Plasmids - CRISPR Transposases (CAST)

    Type
    Collection
    ...Xenopus CRISPR Resources CRISPR Guide Viral Preps Protocols gRNA Design Tools CRISPR Blog Posts CRISPR-associated...CRISPR Plasmids: CRISPR Transposases (CASTs) Browse CRISPR Plasmids By Function Genome Editing Cut Base...Purify Tag Visualize dCas9-FokI Screen Pooled Libraries gRNAs Empty gRNA Vectors gRNAs By Species Mammalian... or search the tables below for CRISPR Transposase plasmids. Plasmids are available for expression in ...Find plasmids for CRISPR-associated transposases (CASTs) for insertion of donor DNA. CRISPR...Mammalian ID Plasmid Gene/Insert Promoter Selectable Marker PI Publication Bacteria ID Plasmid Gene/Insert...suggestions for other plasmids that should be added to this list? Fill out our Suggest a Plasmid form or e-mail...

  5. CRISPR Plasmids - Single-Strand Break (Nick)

    Type
    Collection
    ...Xenopus CRISPR Resources CRISPR Guide Viral Preps Protocols gRNA Design Tools CRISPR Blog Posts CRISPR/Cas...Purify Tag Visualize dCas9-FokI Screen Pooled Libraries gRNAs Empty gRNA Vectors gRNAs By Species Mammalian... CRISPR Nick CRISPR Plasmids: Nick...sort, or search the tables below for CRISPR nickase plasmids. Plasmids are available for expression in mammalian...CRISPR nickase plasmids for the generation of single-strand breaks. CRISPR...Nick Browse CRISPR Plasmids By Function Genome Editing Cut Base Edit Nick Prime Edit Transcriptional Regulation...Mammalian ID Plasmid Gene/Insert Promoter Selectable Marker PI Publication Bacteria ID Plasmid Gene/Insert...

  6. CRISPR Plasmids - Double-Strand Break (Cut)

    Type
    Collection
    ...Zebrafish Xenopus CRISPR Resources CRISPR Guide Viral Preps Protocols gRNA Design Tools CRISPR Blog Posts Fully...Purify Tag Visualize dCas9-FokI Screen Pooled Libraries gRNAs Empty gRNA Vectors gRNAs By Species Mammalian... CRISPR Cut CRISPR Plasmids: Cut Browse CRISPR...search the tables below for CRISPR plasmids designed to introduce a DSB. Plasmids are available for expression...CRISPR plasmids that create double strand breaks in DNA. CRISPR...Mammalian ID Plasmid Gene/Insert Promoter Selectable Marker PI Publication Bacteria ID Plasmid Gene/Insert...Drosophila ID Plasmid Gene/Insert Promoter Selectable Marker PI Publication Plant ID Plasmid Gene/Insert...

  7. CRISPR Plasmids - RNA Editing

    Type
    Collection
    ...Zebrafish Xenopus CRISPR Resources CRISPR Guide Viral Preps Protocols gRNA Design Tools CRISPR Blog Posts Type...Purify Tag Visualize dCas9-FokI Screen Pooled Libraries gRNAs Empty gRNA Vectors gRNAs By Species Mammalian...tables below for CRISPR plasmids for RNA editing in mammalian systems. Mammalian ID Plasmid Gene/Insert PI...CRISPR plasmids based on Cas13 designed to precisely edit RNA targets. CRISPR... CRISPR RNA Editing CRISPR Plasmids...Plasmids: RNA Editing Browse CRISPR Plasmids By Function Genome Editing Cut Base Edit Nick Prime Edit Transcriptional...suggestions for other plasmids that should be added to this list? Fill out our Suggest a Plasmid form or e-mail...

  8. Bikard Lab - CRISPR Repression Collection

    Type
    Collection
    ...Lab - CRISPR Repression Collection Bikard Lab CRISPR Repression Plasmids You may also like... CRISPR Guide...Guide CRISPR Protocols gRNA Design Tools CRISPR Blog Posts The David Bikard Lab at the Institute Pasteur... using a plasmid‐borne CRISPR array coding for guide RNAs (diamonds) interspaced with CRISPR repeat motifs...From the Bikard Lab, CRISPR plasmid collection for modulating expression levels of two targets independently...levels. This collection comprises 20 plasmids, each carrying two CRISPR guides. The first one binds to sfgfp...high‐throughput microscopy for a collection of 20 CRISPR plasmids. Each point represents a single cell, and each...represents the population obtained with one CRISPR plasmid. An overlaid meshwork connects the median values...

  9. Rett Syndrome

    Type
    Collection
    ... using plasmids, please see Addgene's Plasmids for Stem Cell Research page. CRISPR Tools CRISPR technology...selection of plasmids expressing Cas9 and empty gRNA backbones. A few examples of additional CRISPR resources...Addgene's CRISPR Guide for a general introduction to CRISPR technology or the mammalian CRISPR resources... Syndrome Plasmid Collection and Resource Center MECP2 Animal Models Human Cell Lines CRISPR Tools Plasmids...Editing CRISPR-based DNA methylation editing system - Shawn Liu and Rudolf Jaenisch labs Plasmids MECP2 ...MECP2 Plasmids The table below highlights plasmids that express or target the MECP2 gene. Use the search bar...Broswe plasmids and other research tools for use in Rett Syndrome research. Plasmid...

  10. Neurodegeneration Plasmid Collection

    Type
    Collection
    ...Neurodegeneration Neurodegeneration Plasmids Neurodegeneration Plasmid Collection Addgene has a large collection... Codon-Optimized gRNA Other Tags GFP FP other Flag GST His TEV Other, non-FP Plasmid Type Mammalian, non-viral...75637 EIF2AK2 gRNA (BRDN0001146342) EIF2AK2 hU6 VWM disease David Root 75638 EIF2AK2 gRNA (BRDN0001148577...75639 EIF2AK2 gRNA (BRDN0001148668) EIF2AK2 hU6 VWM disease David Root 76195 ERBB4 gRNA (BRDN0001162372...David Root 76196 ERBB4 gRNA (BRDN0001149467) ERBB4 hU6 ALS David Root 76197 ERBB4 gRNA (BRDN0001146197) ERBB4...David Root 76198 ERBB4 gRNA (BRDN0001162324) ERBB4 hU6 ALS David Root 76809 PANK2 gRNA (BRDN0001149459) PANK2...76810 PANK2 gRNA (BRDN0001162474) PANK2 hU6 Hallervorden-Spatz disease David Root 76811 PANK2 gRNA (BRDN0001162362...
Showing: 41 - 50 of 50 results