...Assembly Standards SynBio Blog Genome Engineering Cloning & Genomic Tools Metabolism Networks & Gene Regulation...

...Assembly Standards SynBio Blog Genome Engineering Cloning & Genomic Tools Metabolism Networks & Gene Regulation...

... based on factors such as selectable marker or cloning method. When using CRISPR, you will need to express...Cas-containing plasmids. gRNA Plasmid Promoter Cloning Enzyme(s) Validated In Resistance Co-expressed ...

...Assembly Standards SynBio Blog Genome Engineering Cloning & Genomic Tools Metabolism Networks & Gene Regulation...

... Assembly method Nuclease Expression Vectors - Cloning vectors for expressing zinc finger FokI nuclease...

...using the REAL, REAL-Fast or FLASH methods) can be cloned for expression as TALENs. Additional details about...

...choice (Fig. 1B) . Thus, through standard plasmid cloning, viruses can be engineered to harbor a wide array...easily modified and amplified (through standard cloning methods). There are different types of viruses ...

... based on factors such as selectable marker or cloning method. When using CRISPR, you will need to express...variety of Cas-containing plasmids. Plasmid Promoter Cloning Enzyme Co-expressed Cas9 Cas9 System Selection ...

...targeted to sequences of interest can be made by cloning customized oligonucleotides into pSQT1313 using...

...found in this article: St-Pierre et al., One-Step Cloning and Chromosomal Integration of DNA, ACS Synthetic...

...isoforms and two control vectors. The individual clones, or the pooled library containing the entire pooled...

...using the REAL, REAL-Fast or FLASH methods) can be cloned for expression as TALE transcriptional activators...

... in-frame to any of these vectors using unique cloning sites. Vectors encoding wild-type FokI nuclease...

...Vector The backbone that a target sequence gets cloned into to create the gRNA 41815 hCas9 A human codon-optimized...protocol for synthesizing gRNAs: Cas9 orthologs gRNA cloning protocol 104.1 KB This set of plasmids is described...

...using standard restriction-ligation cloning. However, the exact cloning strategy will depend on the gRNA ...targeted region from DNA, subcloning into a plasmid, and screening individual clones. PCR amplification and...gRNA Know your Cell Line Select Gene Select gRNA Clone gRNA Delivery of gRNA & Cas9 Validate Results Resources... the desired edit experimentally and to isolate clones containing the desired edit (see the validation...to target anywhere from 2 to 7 genetic loci by cloning multiple gRNAs into a single plasmid. These multiplex...silico and synthesized (see panel A below), then cloned in a pooled format into lentiviral transfer vectors...transfer, analogous to traditional restriction enzyme cloning, which may increase the efficiency of gene editing...

... view all kits. Kit Types Fluorescent Proteins Cloning Genome Engineering Pathways & ORFs Synthetic Biology...

...of one clone that are specific to a certain epitope, or polyclonal, meaning there are many clones that ...antibodies. Recombinant antibodies are created by cloning antibody genes from specific hybridoma cells into... bind to any given antigen. A mature B cell can clone itself to enable higher production of a single antibody...where each culture should produce the same antibody clone, or from plasmid-based expression. Monoclonal antibodies...

... plasmid as AAV. We currently do not offer DNA cloning services. To find out more about a specific plasmid...

...AscI at the 3' end. These inserts can then be sub-cloned into a series of expression vectors which have ...

... Anti-SARS-CoV-2 Nucleocapsid Protein [mBG17] - Clone mBG17, recombinant mouse monoclonal antibody targeting... Anti-SARS-CoV-2 Nucleocapsid Protein [mBG86] - Clone mBG86, recombinant mouse monoclonal antibody targeting...