...mutations of these regulatory elements on gene expression. Empty backbones for inserting regulatory elements before... reporter vector via Gateway cloning to test for the presence of transcriptional regulatory region in ...regions Joshua Mendell 212936 pGL3 Basic Vector Firefly Vector for investigating regions controlling transcription...are commonly used to investigate the effect of regulatory elements, such as promoters, enhancers and untranslated...strong constitutive promoter and can be used to monitor transfection or viral infection efficiency, to ...luciferase to assess FKBP and FRP interactions in the mTOR signaling pathway in vivo . DULIP ( DU al L uminescence-based...Optimized STARR-seq ( S elf- T ranscribing A ctive R egulatory R egion) plasmids : Genome-wide screening of enhancer...

...Resources Viral Vector Production Learn about how we produce high-quality viral vector preps at Addgene...Request service with all-in-one pricing for viral vector preps, which includes MTA facilitation, DNA amplification... Viral Vector Packaging Service Packaged on Request AAV Packaged...Additional Resources Adeno-associated virus (AAV) vectors are safe and useful gene delivery tools that effectively... protection levels. We offer 961 in-stock viral vector preps, delivering high quality and ready availability...availability at affordable prices. While our repository serves the needs of many researchers, scientists sometimes... the steps to produce, purify, and titer viral vectors. Overview Our new Packaged on Request service is...

...Nuclear receptor Co-Repressor 1/Silencing Mediator of Retinoic acid and Thyroid hormone receptor (NCoR/...Cell Line Repositories (Link opens in a new window) Coriell Institute - cell line repository, including...in a new window) MMRRC Repository - NIH-supported mouse and cell line repository (Link opens in a new window...largest biorepositories (Link opens in a new window) ATCC - the world's largest cell line repository Tissue... working with the RSRT along with individual laboratories to assemble a Rett Syndrome plasmid resource...The MECP2 protein is a global transcriptional regulator of thousands of genes and studies have suggested...location and type of MECP2 mutation can be a strong predictor of symptom severity. Broadly, early gene disruptions...

...CMV-EGFP reporter cassette is included in the vector to monitor expression; Designed for inducing RNAi in ...shRNA cassette from another vector (e.g. pSUPER) can be cloned into this vector; Contains a 5'LTR and should...Retroviral vector for shRNA expression Bob Weinberg 10675 pMKO.1 puro GFP shRNA Negative control vector for ...protocol for using the popular cloning vector pLKO.1. Trono Lab Lentivectors Toolbox Tyler Jacks Lab Protocols... plasmid. Below, browse our selection of empty vectors for cloning in shRNAs. Plasmids for constitutive...delivery. Constitutive Lentiviral shRNA Lentiviral vectors for constitutive shRNA expression ID Plasmid Description...Description PI 10878 pLKO.1 ‐ TRC cloning vector shRNA expression; Recommended for cloning new shRNAs ...

...targeting vector assembly, and the remaining 5 plasmids include Cre and FLP expression vectors, a general...-FokI Screen Pooled Libraries gRNAs Empty gRNA Vectors gRNAs By Species Mammalian Bacteria Drosophila ...Cas plasmid-system consists of two components: A vector containing Cas9 and a validated gRNA, based on ...tagging system were tested by tagging transcription factors with FLAG in human cell lines. To repeat the tagging...the isolation of native protein complexes. This vector serves as a backbone to clone the left and right...Alternatively, cDNAs can be cloned directly into this vector and targeted to the AAVS1 genomic safe harbor locus...gRNA_AAVS1-T2 (Addgene #41818) or using an all in one vector from the Doyon lab, eSpCas9(1.1)_No_FLAG_AAVS1_...

...Expression Vectors You can use the tables on Addgene's Empty gRNA Vectors page to search based on factors such...specific base editor that can alter DNA bases without inducing a DNA break. Base editors convert C->T ...gRNA sequence to direct the dCas9-activator to promoter or regulatory regions of your gene of interest....-FokI Screen Pooled Libraries gRNAs Empty gRNA Vectors gRNAs By Species Mammalian Bacteria Drosophila ...editing window specified by the gRNA. Adenine base editors convert adenine to inosine, which is replaced by...Catalytically dead dCas9 fused to a transcriptional activator peptide can increase transcription of a specific...separate gRNA expression plasmid to target the dCas9-activator to your specific locus. ID Plasmid Gene/Insert...

... series of expression vectors which have different C-terminal tags. Most vectors are based on the pTT3...encode the extracellular regions of cell surface receptor and secreted proteins as soluble recombinant molecules...important in cellular recognition processes. Laboratories interested in these approaches have created ... screen) in which the extracellular regions of receptor proteins are expressed as both monomeric baits... encode the whole ectodomains of cell surface receptors are flanked by two 8 bp cutting enzymes: NotI ...including positive and negative controls Zebrafish receptors Zebrafish antibody plasmids P. falciparum merozoite...

...and synthetic regulatory elements, including promoters, terminators, repressors, activators, and more. Also... Lab Repressors , Terminators , Light Signaling , Sigma Factors , Resource Allocator and Orthogonal Switches...cerevisiae Advanced Gateway Destination Vectors TAL Effectors Zinc Finger Consortium Community Resources...including shuttle, integration, reporter, and tagging vectors. Metabolism Browse plasmids related to metabolic...Plasmids and Phagemids Balazsi Lab Negative Autoregulation and Yeast-Mammalian Circuit Transfer C. Collins...Plasmid Kit Gray Lab Maize and Rice Transcription Factors Hasty Lab Biopixels Jaramillo Lab Riboregulation...Foundation Registry of Standard Biological Parts - Repository of community-created BioBricks BioBricks Foundation...

... in a single lentiviral transfer vector or separate transfer vectors May contain reporter gene (e.g. GFP...or inserting a tag Cas9 (or Cas9 nickase); Base editor Single (or dual) gRNA targeting the region where...reduced efficiency compared to NHEJ knockout. Base editors can make a limited set of mutations. Repress or...without permanently modifying the genome dCas9-activator (such as dCas9-VP64) gRNA(s) targeting promoter...promoter elements of target gene Many different activators exist, including the multi-plasmid SAM system. Select...Components of System Application Mammalian expression vector Cas enzyme promoter can be constitutive (CMV, EF1alpha...lines (for details, see Plasmids 101: Mammalian Vectors ) Transient or stable expression of Cas9 and/or...

...Expression Vectors for REAL, REAL-Fast and FLASH TALE Transcriptional Activator Expression Vectors for REAL...Reagents from the Keith Joung laboratory for engineering TAL effectors, including designed TALENs, and...TALEN Kits TALengineering Reagents Joung Lab TAL Effector Engineering Reagents You may also like... Keith... developed three platforms for engineering TAL effector repeat arrays of any desired length: the REAL ...2012). All three of these platforms assemble TAL effector repeat arrays on a single architectural framework...all three methods are identical in sequence. TAL effector repeat arrays produced on this framework has yielded..., Nat Biotechnol. 2011 ) TALE transcriptional activators in human cells ( Maeder et al., Nat Methods 2013...

...to an acceptor. Since the transfer of energy does not occur by emission of a photon, the acceptor molecule...and measuring the emission of the acceptor. For a given donor-acceptor pair, the FRET efficiency strongly...constructed using the cpFRET kit from the Pertz laboratory. Empty Vectors Encoding Fluorescent Proteins for FRET...proteins have been developed for use as donor and acceptor pairs in FRET experiments. The efficiency of ... depends on the distance between the donor and acceptor molecules and can therefore be used to calculate...calculate the distances between the donor and acceptor. FRET is often used to study (1) protein-protein interactions...each protein is separately fused to a donor or acceptor molecule (also referred to as intermolecular or...

...Molecular Tools Viral Vector Packaging Service: Molecular Tools Tetracycline Transactivators Affinity Purification...encoding tet-off transactivators and tools for affinity purification (TRAP). Viral... Viral Vector Packaging Service...Neurophysiology Cell Ablation Tetracycline Transactivators and Inducible Tools These AAV encode tetracycline-inducible...-inducible tools/controls and tetracycline transactivators that can be used with tetracycline (tet)-inducible...CAG-driven, constitutive Expression of the tet-off transactivator (tTA) 2 Gradinaru 99120 pAAV-ihSyn1-tTA Inducible... promoter (ihSyn) Expression of the tet-off transactivator (tTA) with a positive feedback loop for amplified...

... gRNA design and cloning gRNA cloning vector Retroviral vectors: neomycin (pSIR-neo) , GFP (pSIR-GFP) ... dCas9 activators sgRNA dual expression: pAC2 , pAC152 , pAC153 , pAC154 ; pmax dCas9 activator expression... pooled library amplification 1 vector system: lentiCRISPR v2 2 vector system: lentiCas9-Blast and lentiGuide-Puro...window) : a webtool that provides models for base editor (BE) gRNA design. DeepPrime (Link opens in a new...window) : a webtool that provides models for prime editor (PE) pegRNA design. PMID 37119812 (Link opens in... the Kim Lab's Cas-OFFinder and Microhomology predictor. CRISPR-ERA: CRISPR -mediated e diting, r epression... and human. CCTop - CRISPR/Cas9 target online predictor Identifies candidate sgRNA target sites by off-target...

...Two classes of base editors — cytosine base editors (CBEs) and adenine base editors (ABEs) — can be used...base editors, the evolved TadA domain is fused to a Cas9 protein to create the adenine base editor. Both...-FokI Screen Pooled Libraries gRNAs Empty gRNA Vectors gRNAs By Species Mammalian Bacteria Drosophila ... without double stranded breaks. Cytosine base editors are created by fusing Cas9 nickase or catalytically...dCas9) to a cytidine deaminase like APOBEC. Base editors are targeted to a specific locus by a gRNA, and...on the opposite strand). Likewise, adenine base editors have been engineered to convert adenosine to inosine...Both types of base editors are available with multiple Cas9 variants including high fidelity Cas9’s. Further...

.... pGCS Vector Kit - Hai-Ning Du Lab. A set of pCS2+ backbone-based Gateway destination vectors (pGCS),...encode zebrafish receptors or monoclonal antibodies to zebrafish cell surface receptors and secreted proteins...found below: UFlip CRISPR targeted integration vectors for cell-type specific conditional gene inactivation...inactivation - Jeffrey Essner Lab GeneWeld Vectors for Targeted Integration Using CRISPR/Cas9 - Jeffrey Essner...mutagenesis in zebrafish - Wenbiao Chen Lab A CRISPR/Cas9 Vector System for Tissue-Specific Gene Disruption in Zebrafish...zebrafish Whole organism lineage tracing by combinatorial and cumulative genome editing - Jay Shendure...with four promoters, six fluorophores, and empty vectors designed to streamline the production of transgenic...

...antibodies can be stored at 4 °C for short-term storage. For long-term storage they should be stored at -20 °C... Antibody Handling Storage Check the manufacturer’s instructions for exact storage requirements. As a ...tips for storing your antibody: Antibodies conjugated to fluorophores should never be stored below 4 °... antibodies should not be stored in sodium azide, as it acts as an inhibitor to HRP. In those cases, thimerosal...define key terms, summarize antibody production and storage techniques, and explain common applications. Science...expensive than polyclonal antibodies. Environmental factors, binding partners, location in the cell, and sample... a primary antibody, which becomes the limiting factor for the number of proteins you can detect in a ...

...cytosine base editor that can alter DNA bases without inducing a DNA break. Cytosine base editors convert C...gRNA sequence to direct the dCas9-activator to promoter or regulatory regions of your gene of interest....Empty gRNA Expression Vectors Select a gRNA expression plasmid based on factors such as selectable marker...-FokI Screen Pooled Libraries gRNAs Empty gRNA Vectors gRNAs By Species Mammalian Bacteria Drosophila ...editing window specified by the gRNA. Adenine base editors convert adenine to inosine, which is replaced by...Catalytically dead dCas9 fused to a transcriptional activator peptide can increase transcription of a specific...separate gRNA expression plasmid to target the dCas9-activator to your specific locus. ID Plasmid Gene/Insert...

...a bacterial stab (of a laboratory E. coli strain). Addgene continues to monitor its library and distributions...Broad-Range Rainbow Vectors - Barbier Lab Cloning and Expression Bacteria and yeast expression vectors : FX cloning...Microbiology Plasmids and Resources Addgene’s repository contains plasmid tools for a range of microbial...plasmid tools for the community that aren't in the repository? Help us widen our resources for the community...protein expression in E. coli, B. subtilis and P. pastoris - Gomis-Rüth Lab pOSIP plasmid kit : Integration...Yarrowia lipolytica Golden Gate tool kit : Integrative vector assembly of up to three transcription units in ...lipolytica - Rossignol Lab Yeast Gateway kit : 285 vectors for expression, markers, fusion proteins, etc -...

...cytosine base editor that can alter DNA bases without inducing a DNA break. Cytosine base editors convert C...gRNA sequence to direct the dCas9-activator to promoter or regulatory regions of your gene of interest....Empty gRNA Expression Vectors Select a gRNA expression plasmid based on factors such as selectable marker...-FokI Screen Pooled Libraries gRNAs Empty gRNA Vectors gRNAs By Species Mammalian Bacteria Drosophila ...editing window specified by the gRNA. Adenine base editors convert adenine to inosine, which is replaced by...Catalytically dead dCas9 fused to a transcriptional activator peptide can increase transcription of a specific...separate gRNA expression plasmid to target the dCas9-activator to your specific locus. ID Plasmid Gene/Insert...

...Publication Empty Prime Editing gRNA Vectors A selection of empty gRNA vectors suitable for prime editing are...tevopreq1-GG-acceptor Mammalian hU6 epegRNA BsaI No mRFP1 David Liu 174039 pU6-tmpknot-GG-acceptor Mammalian...Find prime editor plasmids for (search and replace) genome editing. CRISPR...-FokI Screen Pooled Libraries gRNAs Empty gRNA Vectors gRNAs By Species Mammalian Bacteria Drosophila ...enzyme is named PE1 . PE1 and most other prime editors use the Moloney murine leukemia virus (M-MLV) reverse...can be used in PE2–PE5 systems PE6 - small prime editors with optimized RT and/or Cas9 domains PE7 - addition...addition of RNA-binding exonuclease protection factor La to PEmax to enhance pegRNA stability epegRNA -...