...virus). In the absence of tetracycline, the tetR portion of tTA will bind these tetO sequences and the activation...identify which amino acid residues of tetR were important for tetracycline-dependent repression. Mutating...shRNA efficacy using fluorescence; use with pGFPns-reporter for cDNA target rtTA On Gu 60495 pSBtet-GP Sleeping...

...plasmid webpages. These plasmids are described in Portales-Casamar et al., 2010 . List of Pleiades MiniPromoters...MiniPromoters MiniPromoter Source Gene Construct Reporter Negative Control N/A pEMS1312 cre N/A pEMS1301 cre...intron-lacZ/NLS Additional Resources References Portales-Casamar E, Swanson DJ, Liu L, de Leeuw CN, Banks...

...GRAB_OTmut (control) none Constitutive 1, 9 Li Voltage Reporter: Archon 108422 pAAV-CAG-FLEX-Archon1-KGC-EGFP-...Syn Archon1 EGFP Cre dependent 8 Boyden Voltage Reporter: Voltron 119036 pAAV-hsyn-flex-Voltron-ST Syn ...Voltron2-ST none Cre dependent 1 GENIE Voltage Reporter: JEDI-2P 179459 pAAV-EF1a-DIO-JEDI-2P-Kv-WPRE ...

...involved in cancer development. These pathways are important to normal cell signaling and physiology, and, ...location, their pathway dependencies are extremely important for classification and treatment. On each pathway...

...on each item's material page. Actual titers are reported with each shipment. If you can't find the prep...Biosensors Calcium Sensors, Glutamate Sensors, Voltage Reporters Controls Fluorescent Proteins, Spagehetti Monster...

...Trypanosoma (African sleeping sickness), Cryptosporidium (cryptosporidiosis), Leishmania (leishmaniasis), and...

...accessibility in research. For each antibody, we provide important details including the protein and plasmid sequence...our resources! Acknowledgments This project is supported by a NIH BRAIN Initiative grant (U24NS119916)....

...Effector Molecules (PSEMs). PSAMs are coupled to an ion pore domain to create a ligand-gated ion channel. Whether...PSAM is activating or inhibitory depends on the ion pore domain it is coupled to. Addgene has two main types...

...Where can I find cloning resources or scientific support? Please see our Addgene Protocols for resources... may find helpful. Our Scientific and Customer Support Teams are also available to provide assistance ...

...Resources Addgene's Data Hub Curated technical reports from the research community demonstrating AAV performance...plasmid. Distribution of the original plasmid is important because it is the actual material being referenced...

...set of lentiviral transfer vectors you can find reporter plasmids to test whether or not you've efficiently...to generate adenovirus expressing a variety of reporters as well as the Cre and Flp recombinases. Genes...

...every 35bp. Researchers are still determining the importance of context for each TAL effector within an array...plasmid toolbox. Addgene has rapidly become an important resource for this new technology, and we hope ...

...our resources! Acknowledgments This project is supported by a NIH BRAIN Initiative grant (U24NS119916)....

...protein interaction network and its resolution by spatiotemporal expression profiling. Martin S et al., Mol ...

...systems. Jump to Table: Cloning and Genomic Tools Reporters and Tags Library Construction Integration Recombination...

...choose and use antibodies, with an expert technical support team ready to answer your questions. All Antibodies...

... world. The NETRF is grateful for the generous support of philanthropic individuals and foundations, who...

...proteins as a first step for many different kinds of important experiments. In an effort to speed up research...

...zebrafish Guide RNA expression plasmids for the EGFP Reporter Gene Guide RNA expression plasmids for endogenous...

...microbial opsins, which are membrane channels and pores derived from algae and archaebacteria, that can ...