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Showing: 41 - 60 of 110 results

  1. Caltech Systemic Capsids

    Type
    Collection
    ...control, including AAV titering by probe-based droplet digital PCR, in vitro and (when possible) in vivo...review performance data of each of these serotypes, please refer to Addgene’s Data Hub and the Caltech CLOVER... serotype will work in your mouse line, see Supplementary Table 3 (Link opens in a new window) . Browse...using the Caltech serotypes in future publications, please acknowledge Viviana Gradinaru and cite as follows...would like to see available in viral prep format. Please note this does not guarantee viral vector packaging...

  2. Addgene Packaged on Request: Scope of Service

    Type
    Collection
    ... 12 GC/mL for the AAV2 serotype, as titered by droplet digital PCR (ddPCR) . In some cases, we may expect... sequence verification, may not yet have been completed at the time of shipment. If any discrepancies ...for any additional customs fees or taxes, and to complete any additional customs documentation that might...for the import of biological research materials. Please be aware that additional customs charges may be...

  3. Antibody Plasmid Collection

    Type
    Collection
    ...microscope, or detect when the protein is present in a sample--just to name a few. Traditional sources of antibodies...individual clone targeting a single antigen. An example of R-mAbs is the NeuroMab collection - monoclonal...single-domain antibodies (also known as nanobodies). For example, scientists often use plasmids encoding scFv fragments...recombinant antibody fragments in the form of scFvs A simple vector system to improve performance and utilisation...

  4. Lentiviral Prep Service

    Type
    Collection
    ...vector encoding the MS2-P65-HSF1 activator helper complex with a 2A Hygro resistance marker (EF1a-MS2-p65...Name Insert Antibiotic Resistance Purpose PI 17446 pLenti CMV GFP Hygro (656-4) GFP Hygromycin 3rd gen lentiviral...Suggestions Don’t see what you are looking for? Please let us know what you would like to see available... Suggestion Form (Link opens in a new window) . Please note this does not guarantee viral service, but...

  5. Lentivirus Plasmids

    Type
    Collection
    ...particle (a virion) are generally divided among multiple plasmids. Some plasmids (typically called packaging...lentiviral plasmids to express your CRISPR gRNA? Please see our CRISPR Empty gRNA plasmids table and search... See plasmid 17618 for GFP plasmid. Cheng 17452 pLenti CMV Puro DEST 3rd Gateway destination plasmid with... See article for more variations. Campeau 39481 pLenti-puro 3rd CMV driven expression of cDNA. Puro selection...

  6. COVID-19 Resources

    Type
    Collection
    ...virus Mammalian constructs Due to the size and complexity of the collection, all available Ginkgo SARS-...information on ordering from a for-profit entity, please see our Plasmid Distribution to Industry page. ...top General Plasmid Tools SHERLOCK and DETECTR - Simple, portable, and inexpensive platforms to reliably...Collections of COVID-19 related articles spanning multiple publishers can be found here: ASM COVID-19 Research...

  7. Depositor Collections

    Type
    Collection
    ...Dehydrogenase CUREs Community (MCC) Collection Pleiades Promoter Project: mini-promoters to drive selective...FreeGenes Project Open Enzyme Collection Screening Multiplexed Overexpression of Regulatory Factors (MORF) Collection...Factor Plasmids Terminator Plasmids Viral Vectors pLEG/pREG Modular Viral Vector System - Dankort...

  8. CRISPR Plasmids - CRISPR Transposases (CAST)

    Type
    Collection
    ...Cas enzymes that form a complex called Cascade ( C RISPR- as sociated c omplex for a ntiviral de fense...instead recruiting the transposase (comprised of multiple components) to target locations in the genome ...

  9. CRISPR Plasmids - Cascade-Cas3

    Type
    Collection
    ...Cascade ( C RISPR- as sociated c omplex for a ntiviral de fense) complex, comprised of a combination of ...difficulties in getting all components of the Cascade complex to express equally. To combat this, some CRISPR-Cas3...

  10. SARS-CoV-2 Pseudotyped Virus

    Type
    Collection
    ...the SARS-CoV-2 Spike protein and you will find examples of each in Addgene's collection: HIV-based lentiviral...and Packaging Plasmids Reporter Plasmids A few examples of reporter plasmids that can be used for measuring...expressing mCherry and firefly luciferase. pLentiEGFPdestablized - EFS-EGFPd2PEST-2A-MCS-Hygro - Lentiviral...

  11. Fluorescent Protein Guide: Empty Backbones

    Type
    Collection
    ...Bacterial Expression mApple 568 592 37 6.5 30 min Monomer mApple-N1 - Mammalian Expression mApple-C1 - Mammalian...Mammalian Expression mApple-pBAD - Bacterial Expression mScarlet-I3 568 592 68 4.2 2 min Monomer pmScarlet-...

  12. Microbiology Resources

    Type
    Collection
    ...Ralstonia solanacearum species complex (Rssc) gene knockout and complementation constructs - Lahaye Lab Vaccinia... in genome editing of bacteria. CRISPathBrick Multiplex Plasmid : CRISPR expression in E. coli - Koffas...

  13. Viral Vectors

    Type
    Collection
    ... wide array of genetic functions in cells. For example, if there is a protein thought to be associated..., are suited for specific research goals . For example, adeno-associated virus (AAV) is typically preferred...depending on the insert type and envelope used. Please consult with your institution’s Biosafety Committee...

  14. Tetracycline Inducible Expression

    Type
    Collection
    ...transcriptional activation domain from HSV (herpes simplex virus). In the absence of tetracycline, the tetR...Puromycin was replaced with Blasticidin selection. Please NOTE, this vector contains an insert (MAT2A) which... Plasmid Description Element On or off PI 26429 pLenti CMV rtTA3 Blast (w756-1) Lentiviral reverse tetracycline-controlled...

  15. Penn Vector Core Partnership with Addgene

    Type
    Collection
    ...Plasmids are available for all the items listed below. Please let us know (Link opens in a new window) what you...you would like to see available in viral format. Please note this does not guarantee viral service, but...pENN.AAV.CB7.CI.TurboRFP.WPRE.RBG James M. Wilson Please contact [email protected] with any questions. Return...

  16. AAV Viral Preps

    Type
    Collection
    ...Rabies Virus-Based Serotype Serotype Testing Samples Small samples of control AAV vectors for trial purposes...

  17. Optogenetics Plasmid Collection

    Type
    Collection
    ...plasmids for neuronal excitation and inhibition. For example, search for "ChR mCherry", "Cre inhibition", "Boyden...to control a protein or cellular activity. For example, search "Cdc42", "transcription", "Hahn", or other...

  18. CRISPR Plasmids - gRNAs

    Type
    Collection
    ...and maintained by the Doudna Lab . gRNA Plasmids Please note that the following table lists both published...be used with any CRISPR nuclease and function. Please see the publication or plasmid information page...

  19. Viral Prep Service for Plasmids and Pooled Libraries

    Type
    Collection
    ...Our production process and quality control, with examples of quality control data. Help Center Articles ...will also include virus-associated DNA, which is a sample of the purified DNA from the original plasmid that...

  20. CRISPR Plasmids - Base Edit

    Type
    Collection
    ... Both types of base editors are available with multiple Cas9 variants including high fidelity Cas9’s. ...well suited to directed evolution applications. Examples of these base editing systems include targeted...
Showing: 41 - 60 of 110 results