...RNA-guided transcriptional activator system with two components, the dCas9 activator protein and the single guide...provides the transactivation activity, the sgRNA component provides the specificity and is designed to target...

...γ-Retrovirus (gamma-retrovirus) is an RNA virus comprised of its genome and several structural and enzymatic...gets integrated into the host's genome by the accompanying integrase protein. When scientists discuss retrovirus...

...recombinant antibodies, created by cloning antibody components into plasmids and expressing these in bacteria... and Evolving Functional Proteins. METHODS: A Companion to Methods in Enzymology 8, 94–103 (1995). Carlos...

...different experimental goals. How does this method compare to other similar methods? Where does this method...For All Attendees: To help us best prepare and compile resources for this meeting's publication, we are...

...). A compatible GEF, GTPase, and GAP set is referred to as a minimal triad, with each component involved...

...Ralstonia solanacearum species complex (Rssc) gene knockout and complementation constructs - Lahaye Lab Vaccinia...

...to target mTurquoise2 to various subcellular compartments pCEP4CyPet-MAMM Cyan Mammalian Expresses mammalian... Clover pGWF1 Cyan & Yellow Bacterial Gateway-compatible vector to express a gene of interest fused between...

...Significantly smaller than the above Cas9 protein, NM is comparably active as a nuclease and a transcriptional activator...selection, which revealed that PAM recognition is more complex than previously thought. In particular, combinations...

...and related MECP2 disorders. The RSRT has a comprehensive, strategic and aggressive plan to use gene editing...methylated DNA and the recruitment of the NCoR complex for proper nervous system function. While there...

...Chris Garcia 157115 pD649-HAsp-TREM2-COMP5AP-AviTag-9xHis TREM2 COMP5AP, Avi, His CMV, SP6 Alzheimer's Chris...Chris Garcia 157447 pD649-HAsp-CSF1R-COMP5AP-AviTag-9xHis CSF1R COMP5AP, Avi, His CMV, SP6 Hereditary diffuse...Chris Garcia 157495 pD649-HAsp-ERBB4-COMP5AP-AviTag-9xHis ERBB4 COMP5AP, Avi, His CMV, SP6 ALS Chris Garcia...

...MultiSite Gateway recombination-based cloning of complex polycistronic lentiviruses. Can express Cas9 with... and plasmids that provide Cas9 variants. All components are then assembled into a single T-DNA binary...

...experiments using a pooled CRISPR library are far more complex than using CRISPR to modify a single genomic locus... particular organism, and the library is only compatible with cells derived from that organism. All of...

...biology plasmids related to metabolic pathways and components. Examples Include: Biofuels Natural Product Pathways...

...scientists can design de novo systems using modular components or take advantage of existing GFP-lines for cell-specific...

...are a family of mechanotransduction receptors, comprised of alpha-beta subunit heterodimers. The IPI collection...

...Florida State University has contributed their comprehensive collection of ORFs tagged with a variety of ...

...RNAs” link under the “Support Tools” heading. Completed vectors express a customized guide RNA from a ...

...RNAs” link under the “Support Tools” heading. Completed vectors express a customized guide RNA from a ...

...a range of expression levels. This collection comprises 20 plasmids, each carrying two CRISPR guides. ...

... targeted genome editing. RGNs consist of two components: a short ~100 nt guide RNA ( gRNA ) that uses...