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Showing: 41 - 60 of 90 results

  1. CRISPR Plasmids - Empty gRNA Vectors

    Type
    Collection
    ...S. pyogenes Kamoun pUC119-gRNA 52255 Plant PCR template none S. pyogenes Sheen pRGEB31 51295 Plant BsaI...62201 Plant yes, cut S. pyogenes Hyg Chen pUC57-Simple-gRNA backbone 51306 Xenopus BsaI none S. pyogenes...Bacteria BsaI yes, activate S. pyogenes Marraffini pSimpleII-U6-tracr-U6-BsmBI-NLS-NmCas9-HA-NLS(s) 47868 Mammalian...MultiSite Gateway recombination-based cloning of complex polycistronic lentiviruses. Can express Cas9 with...

  2. Neurodegeneration Research Collection

    Type
    Collection
    ...loci seem to be associated with the disease and implicate a wide range of cellular processes. The vast majority...variation and lifestyle or environmental factors. For example, variations of Apolipoprotein E (APOE), such as...collection of neurological mouse models including complex and monogenic diseases. Alzforum (Link opens in...

  3. COVID-19 Resources

    Type
    Collection
    ...virus Mammalian constructs Due to the size and complexity of the collection, all available Ginkgo SARS-...top General Plasmid Tools SHERLOCK and DETECTR - Simple, portable, and inexpensive platforms to reliably...Andrew Ellington's lab. High-surety isothermal amplification and detection of SARS-CoV-2, including with ...

  4. Rett Syndrome

    Type
    Collection
    ...methylated DNA and the recruitment of the NCoR complex for proper nervous system function. While there...expressing Cas9 and empty gRNA backbones. A few examples of additional CRISPR resources can be found below...cell line repository (Link opens in a new window) Sampled - one of the world's largest biorepositories (Link...

  5. CRISPR Pooled gRNA Libraries

    Type
    Collection
    ...using a pooled CRISPR library are far more complex than using CRISPR to modify a single genomic locus...screening experiments require electroporation to amplify the pooled library and the use of Next-Generation...3rd 10 188,509 Yeast Inducible CRISPRi Library – Ampl133 161829 Inhibition Yeast Tavazoie N/A 6-12 51,681...

  6. Plasmids for Stem Cell Research

    Type
    Collection
    ...generate iPSCs. The generation of iPSCs is relatively simple in concept: ectopically express a cocktail of stem...and Xeno-Free Culture Conditions: A Clinically Compliant Approach. Stem Cells Transl Med. 2015 Mar 5. pii...Lentiviral Mouse Direct Induction of the Three Pre-implantation Blastocyst Cell Types from Fibroblasts. Cell...

  8. CRISPR Plasmids - Xenopus

    Type
    Collection
    ...possibly resulting in frameshifts. If a repair template with high homology to the DNA surrounding the ...Cas9 species = S. pyogenes (PAM = NGG) 51306 pUC57-Simple-gRNA backbone T7 BsaI In vitro transcription none...

  10. AAV Viral Preps

    Type
    Collection
    ...Rabies Virus-Based Serotype Serotype Testing Samples Small samples of control AAV vectors for trial purposes...

  11. CRISPR Plasmids - Double-Strand Break (Cut)

    Type
    Collection
    ...changes, researchers use ssDNA or dsDNA repair templates with 1. homology to the DNA flanking the DSB and...edit close to the gRNA PAM site. When a repair template is present, the cell may repair a DSB using homology-directed...

  12. CRISPR Plasmids - Zebrafish

    Type
    Collection
    ...changes, researchers use ssDNA or dsDNA repair templates with homology to the DNA flanking the DSB and ...edit close to the gRNA PAM site. When a repair template is present, the cell may repair a DSB using homology-directed...

  13. CRISPR Plasmids - Parasites

    Type
    Collection
    ...changes, researchers use ssDNA or dsDNA repair templates with homology to the DNA flanking the DSB and ...edit close to the gRNA PAM site. When a repair template is present, the cell may repair a DSB using homology-directed...

  14. Optogenetics Plasmid Collection

    Type
    Collection
    ...plasmids for neuronal excitation and inhibition. For example, search for "ChR mCherry", "Cre inhibition", "Boyden...to control a protein or cellular activity. For example, search "Cdc42", "transcription", "Hahn", or other...

  15. DNA Service - Cloning Grade DNA

    Type
    Collection
    ...reducing the time to experiments by removing the amplification and extraction steps required when one receives...through our website. Every order will need to be completed independently including the check out and will...

  16. Viral Prep Service for Plasmids and Pooled Libraries

    Type
    Collection
    ...Our production process and quality control, with examples of quality control data. Help Center Articles ...will also include virus-associated DNA, which is a sample of the purified DNA from the original plasmid that...

  17. AAV Molecular Tools

    Type
    Collection
    ...transactivator (tTA) with a positive feedback loop for amplified tTA expression. 1 Gradinaru 99121 pAAV-ihSyn1-...transactivator (tTA) with a positive feedback loop for amplified tTA expression. 1 Gradinaru 117383 TRE-DIO-eYFP...

  18. SARS-CoV-2 Pseudotyped Virus

    Type
    Collection
    ...the SARS-CoV-2 Spike protein and you will find examples of each in Addgene's collection: HIV-based lentiviral...and Packaging Plasmids Reporter Plasmids A few examples of reporter plasmids that can be used for measuring...

  19. CRISPR Plasmids - C. elegans

    Type
    Collection
    ...changes, researchers use ssDNA or dsDNA repair templates with homology to the DNA flanking the DSB and ...edit close to the gRNA PAM site. When a repair template is present, the cell may repair a DSB using homology-directed...

  20. Addgene Packaged on Request: Scope of Service

    Type
    Collection
    ... sequence verification, may not yet have been completed at the time of shipment. If any discrepancies ...for any additional customs fees or taxes, and to complete any additional customs documentation that might...
Showing: 41 - 60 of 90 results