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Showing: 41 - 60 of 110 results
  1. Bacterial Expression Systems

    Type
    Collection
    ...bind to a small molecule, the reporter plasmid can be used to detect the presence of that small molecule... have been well studied or can be controlled by small molecules, light, temperature, or even intracellular...plasmid. Other similar reporters for detecting maltose can be found here . pAra Series Various Plasmids...
  2. CRISPR Pooled gRNA Libraries

    Type
    Collection
    ...Libraries gRNAs Empty gRNA Vectors gRNAs By Species Mammalian Bacteria Drosophila Plant C. elegans Yeast Zebrafish...Human Bradley 3rd ~9 pairs/exon 9,508 Mouse CRISPR Proximal Poly(A) Site Knockout Library 190744 Knockout ... 6 Varies Enriched subpools (kinase, nuclear, ribosomal, cell cycle) 51043 — 51048 Knockout Human Sabatini...Pan-Druggable Cancer Library 182133 Knockout Human Mali 3rd 1 74 Perturb-seq Guide Barcodes (GBC) 85968 ...
  3. CRISPR Plasmids - dCas9-FokI

    Type
    Collection
    ... available for expression in mammalian systems and Drosophila. Mammalian ID Plasmid Gene/Insert Promoter...Libraries gRNAs Empty gRNA Vectors gRNAs By Species Mammalian Bacteria Drosophila Plant C. elegans Yeast Zebrafish...
  4. CRISPR Plasmids - Epigenetics

    Type
    Collection
    ... for epigenetic modification in mammalian or plant systems. Mammalian ID Plasmid Gene/Insert Promoter ...Libraries gRNAs Empty gRNA Vectors gRNAs By Species Mammalian Bacteria Drosophila Plant C. elegans Yeast Zebrafish...
  5. CRISPR Plasmids - Repress Gene Expression

    Type
    Collection
    ...available for expression in mammalian systems, bacteria, plants, and yeast. Mammalian ID Plasmid Gene/Insert...Libraries gRNAs Empty gRNA Vectors gRNAs By Species Mammalian Bacteria Drosophila Plant C. elegans Yeast Zebrafish...
  6. CRISPR Plasmids - Activate Gene Expression

    Type
    Collection
    ...for expression in mammalian systems, bacteria, Drosophila, plants, and yeast. Mammalian ID Plasmid Gene/...Libraries gRNAs Empty gRNA Vectors gRNAs By Species Mammalian Bacteria Drosophila Plant C. elegans Yeast Zebrafish...
  7. Plasmids for Stem Cell Research

    Type
    Collection
    ...tracking of reprogramming factors Fluorescent tagged episomals for stoichiometric induced pluripotent stem cell...Fibroblasts Cholinergic Neurons Lentiviral Human Small molecules enable neurogenin 2 to efficiently convert... Cell Rep. 2016 Jan 5;14(1):115-28. Zhang Adult Dermal Fibroblasts Neurons Lentiviral Human REST suppression...
  8. CRISPR Plasmids - Single-Strand Break (Nick)

    Type
    Collection
    ...for expression in mammalian systems, bacteria, Drosophila, plants, and yeast. Mammalian ID Plasmid Gene/...Libraries gRNAs Empty gRNA Vectors gRNAs By Species Mammalian Bacteria Drosophila Plant C. elegans Yeast Zebrafish...
  9. CRISPR Plasmids - Purify Genomic Loci

    Type
    Collection
    ...available for expression in mammalian systems, bacteria, and yeast. Mammalian ID Plasmid Gene/Insert Promoter...Libraries gRNAs Empty gRNA Vectors gRNAs By Species Mammalian Bacteria Drosophila Plant C. elegans Yeast Zebrafish...
  10. Fluorescent Protein Guide

    Type
    Collection
    ...conformational changes within a protein. Biosensors Monitor small biomolecules or other physiological intracellular... or sensors. Chemogenetics Plasmids | Guide Use small molecules to activate genetically engineered cellular...protein-protein interactions in organs and whole mammals. Regulate Biological Activity Use fluorescent proteins...
  11. AAV Viral Preps

    Type
    Collection
    ...delivery tools that effectively transduce many mammalian cell types. AAV is used in a wide range of experiments...in-stock AAV preps are affordable, available in small volumes, and ready to ship. All viral vector preps...Rabies Virus-Based Serotype Serotype Testing Samples Small samples of control AAV vectors for trial purposes...
  12. CRISPR Plasmids - Double-Strand Break (Cut)

    Type
    Collection
    ...Libraries gRNAs Empty gRNA Vectors gRNAs By Species Mammalian Bacteria Drosophila Plant C. elegans Yeast Zebrafish...DSB. Plasmids are available for expression in mammalian systems, bacteria, Drosophila, plants, C. elegans...elegans, yeast, zebrafish, and Xenopus. Mammalian ID Plasmid Gene/Insert Promoter Selectable Marker PI Publication...
  13. Chemogenetics Plasmids

    Type
    Collection
    ...engineered receptors that are activated by specific small molecules, allowing a scientist to control signaling...engineered to respond to specific physiologically inert small molecules. There are four types DREADDs in Addgene...receptor (nAChR) domains that respond specifically to small molecules termed Pharmacologically Selective Effector...
  14. CRISPR Plasmids - Cascade-Cas3

    Type
    Collection
    ...available for expression in mammalian systems, bacteria, and plants. Mammalian ID Plasmid Gene/Insert Promoter...Libraries gRNAs Empty gRNA Vectors gRNAs By Species Mammalian Bacteria Drosophila Plant C. elegans Yeast Zebrafish...
  15. Fluorescent Protein Guide: Subcellular Localization

    Type
    Collection
    ...pcDNA3.1-kappa-myc-dL5-2xG4S-TMst Cell surface (mammalian) PDGFR-derived dL5 FAP; mCerulean3 Marcel Bruchez...Dammeyer 54520 mCherry-Peroxisomes-2 Peroxisome Peroximal Targeting Signal 1 (PTS1) mCherry* Michael Davidson...Davidson 36203 pmTurquoise2-Peroxi Peroxisome Peroxisomal Targeting Sequence mTurquoise2 Dorus Gadella ...responsible for forming connections and adhesions between animal cells. They are important for holding cell structures...
  16. Zhang Lab's CRISPR Frequently Asked Questions

    Type
    Collection
    ...than 100bp away, ideally less than 10bp away. For small (<50bp) changes, you should consider using ssDNA...less than 10bp away if possible. For introducing small mutations (<50bp) or a single-point mutation, the...length in drosophila. This 1998 study suggests mammalian cells' tract lengths seem much shorter. What is...
  17. Neurodegeneration Plasmid Collection

    Type
    Collection
    ...VWM disease Harmit Malik 20045 pSB819-PKR-hum-FFS EIF2AK2 GAL VWM disease Harmit Malik 20046 pSB819-PKR-...VWM disease Harmit Malik 20047 pSB819-PKR-hum-LYS EIF2AK2 GAL VWM disease Harmit Malik 20048 pSB819-PKR-...VWM disease Harmit Malik 20049 pSB819-PKR-hum-FYA EIF2AK2 GAL VWM disease Harmit Malik 20050 pSB819-PKR-...VWM disease Harmit Malik 20051 pSB819-PKR-hum-LFA EIF2AK2 GAL VWM disease Harmit Malik 20123 PCRII-zSynC...other Flag GST His TEV Other, non-FP Plasmid Type Mammalian, non-viral Lentiviral AAV Retroviral Bacterial... Mark Mayford 35000 Nurr1 NR4A2 PGK Parkinson's Malin Parmar 35217 psen2_L (OZ577) PSEN2 lac UV5 Alzheimer's...
  18. Trimmer Lab NeuroMab Collection

    Type
    Collection
    .../28R] HCN1 Rat Mouse IgG2a 114513 Anti-Malin [N85/18.1R] Malin Human Mouse IgG2a 114514 Anti-DNMT3L [N117...by transiently transfecting the plasmids into mammalian tissue culture cells (e.g., HEK293, COS, CHO),...can be used to produce R-mAbs from transfected mammalian cells. Addgene ID Plasmid Target Target Species...GSG2 Human Mouse IgG2a 206531 Anti-MBP [N128A/4R] Maltose binding protein (MBP) E. coli Mouse IgG2a 206532...can be used to produce scFvs from transfected mammalian cells. Addgene ID Plasmid Hybridoma Target Target... as intrabodies and nanoscale immunolabels in mammalian brain neurons. eLife . 8:e48750. PMID: 31566565...
  19. TALEN Expression Vectors

    Type
    Collection
    ...Mammalian expression vectors for TAL effector repeat arrays assembled using the REAL, REAL-Fast or FLASH...vectors listed below have the CMV promoter for mammalian cell expression and a T7 promoter for in vitro...
  20. CRISPR Plasmids for Genomic Visualization

    Type
    Collection
    ...Libraries gRNAs Empty gRNA Vectors gRNAs By Species Mammalian Bacteria Drosophila Plant C. elegans Yeast Zebrafish... with target sites throughout the chromosome. Mammalian ID Plasmid Gene/Insert Promoter Selectable Marker...
Showing: 41 - 60 of 110 results