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Showing: 41 - 60 of 100 results

  1. Optogenetics AAV Preps

    Type
    Collection
    ... GFP Red-wavelength fluorescent protein Yellow-wavelength fluorescent protein Blue-wavelength fluorescent...

  2. Bacterial Expression Systems

    Type
    Collection
    ... Systems Bacterial Expression Systems Genome Engineering Visualization and Tagging Purification Controlled... expression systems to find out more! Genome Engineering Although overexpressing or knocking down a gene...purified protein. Protein purification can be challenging, but once you get it right, you can learn many...

  3. KLF Research Plasmids

    Type
    Collection
    ...Research KLF Depositing Labs Juan Belmonte Linzhao Cheng Jose Cibelli George Daley Jin-Tang Dong Steven Dowdy...Townes Robert Weinberg Knut Woltjen Shinya Yamanaka Feng Zhang About The Krüppel-like factors (KLFs) are ...

  4. TALEN Expression Vectors

    Type
    Collection
    ... TALengineering Reagents TALEN Expression Vectors TALEN Expression... FLASH assembly methods can be found on the TALengineering website . All three of these methods yield ...

  5. CRISPR Plasmids and Resources

    Type
    Collection
    ...immune system to a revolutionary tool for genome engineering. CRISPR Topic Page : Want the latest news on ...experiments. Browse CRISPR Plasmids by Function Genome Engineering Cut Wild-type Cas enzymes generate double strand...

  6. Microbiology Resources

    Type
    Collection
    ...gondii Trypanosoma sp. Plasmids for Viruses Species Dengue Hepatitis Influenza Mumps virus Rubeola (measles...Read our CRISPR Methods for Bacterial Genome Engineering blog post to see how Addgene depositors are making...

  7. Zebrafish Plasmid Collection

    Type
    Collection
    ...and non-coding RNAs in zebrafish - Tatjana Sauka-Spengler Lab Lineage Tracing Cell lineage tracing in multicellular...drivers in vivo. Biotagging toolkit - Tatjana Sauka-Spengler Lab. A genetic binary system for cell- and compartment-specific...

  8. Open Enzyme Collection

    Type
    Collection
    ...functionality or add new functions through genetic engineering and directed evolution. More information about...polymerase 165543 pOpen-Bstpol Bst DNA Polymerase, Full Length 165544 pOpen-T5gene122 T5 DNA Polymerase 165553...

  10. COVID-19 Resources

    Type
    Collection
    ...Finding nucleic acids with SHERLOCK and DETECTR . Feng Zhang's lab at the Broad Institute has utilized ...Center The Lancet COVID-19 Resource Centre The New England Journal of Medicine Coronavirus webpage PLOS Journals...

  11. Plan Your Experiment

    Type
    Collection
    ...gRNAs that have been used successfully in genome engineering experiments. Using validated gRNAs can save your...Publications Cong, L., & Zhang, F. (2015). Genome engineering using CRISPR-CAS9 system. Methods in Molecular...

  12. Genomic Deletions in Mammalian Cell Lines

    Type
    Collection
    ...re-purposed for site-specific eukaryotic genome engineering. CRISPR/Cas9 is an inexpensive, facile, and efficient...manuscript here: Bauer et al, 2014 322.2 KB Genome engineering using the CRISPR-Cas9 system. Ran FA...

  13. CRISPR Pooled gRNA Libraries

    Type
    Collection
    ...192824 Knockout Mouse Knouse 3rd ~5 71,878 Mouse messenger-RBP sgRNA Library 169082 Knockout Mouse Turner... gondii Lourido N/A 10 8,158 Turner Lab Human messenger-RBP sgRNA Library 168791 Knockout Human Turner...

  14. Plasmids for Stem Cell Research

    Type
    Collection
    ...Cells Transl Med. 2015 Mar 5. pii: sctm.2014-0214. Cheng RNA Human Reprogramming factors for delivering synthetic...expression signatures. Cell Res. 2011 Mar;21(3):518-29. Cheng piggyBac Mouse Doxycycline-inducible piggyBac transposon...

  15. Fluorescent Protein Guide: Subcellular Localization

    Type
    Collection
    ...fluorescent protein that has a different emission wavelength. For example, if the marker protein is fused ...Patricia Wadsworth 27123 eTC GFP beta-actin full length Actin Filaments beta-actin EGFP Robert Singer 27382...

  16. Fluorescent Protein Guide: Empty Backbones

    Type
    Collection
    ... Gene, 1992 ), and since then scientists have engineered numerous GFP-variants and non-GFP proteins that...Photoactivatable (e.g. off to on) Excitation and Emission wavelengths after activation. Protein Excitation (nm) Emission...

  17. Genetic Code Expansion

    Type
    Collection
    ...Because there are no free codons, this can be challenging. In E.coli , the rarest codon is the amber stop...the ncAA in the media to make sure that the full length protein is only made when the ncAA is included....

  19. Synthetic Biology - Algal

    Type
    Collection
    ...SynBio Assembly Standards SynBio Blog Genome Engineering Browse Addgene's collection of synthetic biology...

  20. Synthetic Biology - Bacterial

    Type
    Collection
    ...SynBio Assembly Standards SynBio Blog Genome Engineering Browse Addgene's collection of synthetic biology...
Showing: 41 - 60 of 100 results