...tracrRNA: This plasmid contains three expression cassettes. To target a given site, the plasmid can be digested...adenocarcinoma pathology. These plasmids as well as a backbone plasmid for cloning new targets are described...sgRNA backbone for cloning new targeted plasmids. The plasmid can be digested using SapI, which will reveal...2015 Apr 1. PubMed . Return to AAV In vivo plasmids AAV Plasmids with Cas9 Mouse CRISPR-Cas9 knockin mice... Cas9 gRNA plasmids from the Zhang lab Genome...

...Activate CRISPR Plasmids: Activate Browse CRISPR Plasmids By Function Genome Editing Cut Base Edit Nick...the tables below for CRISPR plasmids for transcriptional activation. Plasmids are available for expression.... Mammalian Plasmid Gene/Insert Promoter Selectable Marker PI Publication Bacteria Plasmid Gene/Insert...Publication Drosophila Plasmid Gene/Insert Promoter PI Publication Plant Plasmid Gene/Insert Promoter Selectable...Publication C. elegans Plasmid Gene/Insert Promoter PI Publication Yeast Plasmid Gene/Insert Promoter Selectable...suggestions for other plasmids that should be added to this list? Fill out our Suggest a Plasmid form or e-mail...CRISPR plasmids for activating or upregulating gene expression in various model organisms. CRISPR...

...efficiency than NHEJ. Plasmid Gene/Insert Promoter PI Publication Browse CRISPR Plasmids By Function Genome...gRNA expression plasmid to target the dCas9-activator to your specific locus. Plasmid Gene/Insert Promoter...gRNA expression plasmid to target the dCas9-repressor to your specific locus. Plasmid Gene/Insert Promoter...paired with a wide variety of Cas-containing plasmids. gRNA Plasmid Promoter Cloning Enzyme(s) Validated In...Ampicillin none, need Cas9 plasmid Qi Do you have suggestions for other plasmids that should be added to ...CRISPR plasmids that have been designed for use in bacteria. CRISPR... CRISPR Bacteria CRISPR Plasmids: Bacteria...

...gRNA expression plasmid to target the dCas9-activator to your specific locus. Plasmid Gene/Insert Promoter...gRNA expression plasmid to target the dCas9-repressor to your specific locus. Plasmid Gene/Insert Promoter...paired with a wide variety of Cas-containing plasmids. gRNA Plasmid Promoter Cloning Enzyme(s) Validated In...cerevisiae LEU2 none, need Cas9 plasmid Lu Do you have suggestions for other plasmids that should be added to ...CRISPR plasmids that have been designed for use in yeast. CRISPR... CRISPR Yeast CRISPR Plasmids: Yeast Browse ...Browse CRISPR Plasmids By Function Genome Editing Cut Base Edit Nick Prime Edit Transcriptional Regulation ...

...the tables below for CRISPR plasmids designed to introduce a DSB. Plasmids are available for expression.... Mammalian Plasmid Gene/Insert Promoter Selectable Marker PI Publication Bacteria Plasmid Gene/Insert...Publication Drosophila Plasmid Gene/Insert Promoter Selectable Marker PI Publication Plant Plasmid Gene/Insert Promoter...Publication C. elegans Plasmid Gene/Insert Promoter Selectable Marker PI Publication Yeast Plasmid Gene/Insert Promoter...Publication Zebrafish Plasmid Gene/Insert Promoter Selectable Marker PI Publication Xenopus Plasmid Gene/Insert ...suggestions for other plasmids that should be added to this list? Fill out our Suggest a Plasmid form or e-mail...CRISPR plasmids that create double strand breaks in DNA. CRISPR...

...Accessory Plasmids Envelope and Packaging Plasmids Popular Lentiviral Envelope and Packaging Plasmids Popular...Envelope and Packaging Plasmids Reporter Plasmids A few examples of reporter plasmids that can be used for...particles See Addgene's Viral Plasmids and Resources page for more information. Plasmids Expressing Spike and ...Addgene plasmid #141347) is available in standard format and as a Lentiviral prep . ID Plasmid Description...COVID-19 and Coronavirus Plasmids & Resources Ginkgo Bioworks COVID-19 Plasmid Collection Return to top...suggestions for other plasmids that should be added to this list? Fill out our Suggest a Plasmid form or e-mail...Addgene's collection of plasmids for viral pseudotyping with SARS-CoV-2 spike protein. Collections...

... Backbones Plasmid Description Industry mEmeralds Plasmid Description Industry Reds Plasmid Description...Industry Blues Plasmid Description Industry Cyans Plasmid Description Industry Oranges Plasmid Description...Greens Plasmid Description Industry Yellows Plasmid Description Industry Photoactivatables Plasmid Description...suggestions for other plasmids that should be added to this list? Fill out our Suggest a Plasmid form or e-mail... Plasmid Collections Fluorescent Proteins Michael Davidson...

...the tables below for CRISPR plasmids for transcriptional inhibition. Plasmids are available for expression.... Mammalian Plasmid Gene/Insert Promoter Selectable Marker PI Publication Bacteria Plasmid Gene/Insert...Publication Plant Plasmid Gene/Insert Promoter Selectable Marker PI Publication Yeast Plasmid Gene/Insert Promoter...suggestions for other plasmids that should be added to this list? Fill out our Suggest a Plasmid form or e-mail...CRISPR plasmids for transcriptional repression of target genes. CRISPR... CRISPR Interfere CRISPR Plasmids: Interfere...Interfere Browse CRISPR Plasmids By Function Genome Editing Cut Base Edit Nick Prime Edit Transcriptional...

...Cell-Imaging Plasmid Collection New England Biolabs Cell-Imaging Plasmid Collection Technology Plasmids Supplemental...Biolabs has deposited plasmids at Addgene to generate SNAP, CLIP, ACP or MCP tagged plasmids for visualization...synthases needed for use of these tags. Plasmids Addgene ID Plasmid Substrate Feature 101137 pSNAP-tag (T7...Control Plasmid SNAP-tag Control for mitochondrial localization 101124 pSNAPf-H2B Control Plasmid SNAP-tag...Control Plasmid CLIP-tag Control for nuclear localization 101130 pCLIPf-Cox8A Control Plasmid CLIP-tag...Control Plasmid ACP/MCP-tag Control for cell surface localization 101131 pACP-GPI Control Plasmid ACP/MCP-tag... of proteins inside living or fixed cells. The plasmids associated with this technology are available ...

...to view all plasmids available from the article. Click on the PI link to view all plasmids available from... all other plasmids from that article. Click on the PI link to view all available plasmids from this ...suggestions for other plasmids that should be added to this list? Fill out our Suggest a Plasmid form or e-mail...Synthetic Biology plasmids for cloning and genomic tools. Plasmid... Plasmid Collections Synthetic Biology Cloning...Browse Addgene's collection of synthetic biology plasmids related to Cloning and Genomic Tools for library...Construction Integration Recombination Shuttle Vectors Plasmids for Cloning and Genomic Tools Search the table...

...paired with a wide variety of Cas-containing plasmids. gRNA Plasmid Promoter Cloning Enzyme(s) Delivery Resistance...transcription none, need Cas9 plasmid Chen Do you have suggestions for other plasmids that should be added to...CRISPR plasmids that have been designed for use in Xenopus. CRISPR... CRISPR Xenopus CRISPR Plasmids: Xenopus Browse... Browse CRISPR Plasmids By Function Genome Editing Cut Base Edit Nick Prime Edit Transcriptional Regulation...Design Tools CRISPR Blog Posts The following CRISPR plasmids have been designed for use in Xenopus. Cut Fully...DSB is introduced along with the Cas9 and gRNA plasmids, the cell may instead repair the break using homology-directed...

...paired with a wide variety of Cas-containing plasmids. gRNA Plasmid Promoter Cloning Enzyme(s) Delivery Resistance...need Cas9 plasmid Chen and Wente DR274 T7 BsaI In vitro transcription none, need Cas9 plasmid Joung Do ...suggestions for other plasmids that should be added to this list? Fill out our Suggest a Plasmid form or e-mail...CRISPR plasmids that have been designed for use in zebrafish. CRISPR... CRISPR Zebrafish CRISPR Plasmids: Zebrafish...Zebrafish Browse CRISPR Plasmids By Function Genome Editing Cut Base Edit Nick Prime Edit Transcriptional ...Design Tools CRISPR Blog Posts The following CRISPR plasmids have been designed for use in zebrafish. Cut Fully...

...CRISPR Plasmids: Mammalian Expression The following CRISPR plasmids have been designed for use in mammalian...than NHEJ. Plasmid Gene/Insert Promoter Selectable Marker PI Publication Browse CRISPR Plasmids By Function...gRNA expression plasmid to target the dCas9-activator to your specific locus. Plasmid Gene/Insert Promoter...gRNA expression plasmid to target the dCas9-repressor to your specific locus. Plasmid Gene/Insert Promoter...variety of Cas-containing plasmids. Do you have suggestions for other plasmids that should be added to ...CRISPR plasmids that have been designed for use in mammalian systems. CRISPR...populations of mutants for directed evolution. Plasmid Gene/Insert Promoter Selectable Marker PI Publication...

...Collections Synthetic Biology SynBio Plasmids Synthetic Biology: SynBio Plasmids SynBio Resources SynBio Home ...list of available plasmids related to synthetic biology below. If there are plasmids you would like to...to view all plasmids available from the article. Click on the PI link to view all plasmids available from...Addgene plasmids for use in synthetic biology applications, or from synthetic biology labs. Plasmid... Plasmid Collections...please email [email protected] . Synthetic Biology Plasmids Search the table by keyword or sort by the table... from this depositor's lab. Plasmid Description Gene/Insert Vector Type PI Publication...

...Genomics Consortium (SGC) Plasmids Opher Gileadi Lab plasmids Haian Fu Lab plasmids The TREAT-AD centers are...Plasmid Collections TREAT-AD Plasmid Collection TREAT-AD Plasmid Collection You may also like... Structural...to see all of the TREAT-AD plasmids that are currently available. ID Plasmid Gene/Insert Vector Type Backbone...Browse plasmids supporting the evaluation of a diverse set of novel Alzheimer's disease targets. Plasmid...high-quality research tools are shared openly. Plasmids from Emory-Sage-SGC and IUSM-Purdue TREAT-AD centers...

... Plasmid Collections Empty Backbones Choosing Your Perfect Plasmid Backbone Background... Plasmids - More LIC bacterial cloning vectors pTD plasmid ... plasmids Fly UAS, MT Targeted Gene Expression - Rubin lab plasmids for drosophila...of your cells will take up your plasmid. Thus, many plasmids have markers on them so that you...collection of plasmids for packaging and expression. See our dedicated lentiviral plasmid page . ... plasmid for mammalian gene silencing pLVTHM - lentiviral plasmid for mammalian...suggestions for other plasmids that should be added to this list? Fill out our Suggest a Plasmid form or e-mail...

...of KLF plasmids and learn more about the scientists who have created and shared these plasmids....Plasmid Collections KLF Plasmids Plasmids for KLF Research KLF Depositing Labs Juan Belmonte Linzhao Cheng...information on available plasmids, references, and links. KLF1 KLF2 KLF4 KLF5 KLF6 KLF Plasmids Browse Addgene'...Addgene's collection of KLF plasmids. ID Plasmid Description Gene/Insert PI...shared resource on Addgene. If you have created plasmids containing KLF, we encourage you to contribute...resource. Learn about the benefits of depositing plasmids with Addgene or email [email protected] to get...

... Plasmid Collections EXPRESs Plasmids EXtracellular Plasmid RESource (EXPRESs) Consortium...commonly-used bait and prey plasmids can be found here: Plasmid Type Plasmid Name Bait Cd4d3+4-bio Prey...antibody plasmids P. falciparum merozoite surface protein plasmids P. vivax merozoite protein plasmids Human... Gavin Wright Lab plasmids EXPRESs is a collection of mammalian expression plasmids that encode the extracellular...Browse EXPRESs Consortium plasmids that encode the extracellular regions of cell surface receptor and...facilitate open and free exchange of these expression plasmids. It is hoped that this will encourage other groups...to join our community and contribute their own plasmids in the future. AVEXIS: One popular method to detect...

...Collections Antibody Plasmid Collection Antibody Plasmid Collection Addgene distributes ready-to-use recombinant... table highlights plasmid collections that can be used to create and express plasmid-based antibodies....Apr 8. Markus Seeger Antibody Plasmids The table below highlights plasmids that feature expression of antibodies...) ID Plasmid Description Gene/Insert PI Return to top Do you have suggestions for other plasmids that ...collection of plasmid-based antibodies, including monoclonal antibodies, scFvs, and nanobodies. Plasmid... Plasmid Collections...recombinant antibodies made from plasmids in our collection! Many of these monoclonal antibodies are produced...

... CRISPR Qi Lab CRISPR Plasmids Qi Labs CRISPR Plasmids Available from... genome. 1. Two-plasmid CRISPRi system for bacterial gene knockdown The first plasmid ( pdCas9_bacteria... both plasmids in bacteria could cause up to 300-fold repress on targeted genes. 2. Two-plasmid CRISPRi... 23849981 . Individual plasmids can be ordered via the links below: ID Plasmid Description 44246 pdCas9...fusion expression plasmid 44248 pgRNA-humanized A customizable gRNA expression plasmid for use with either...bacterial Cas9 expression plasmid 44250 pwtCas9-bacteria A wild-type Cas9 expression plasmid for use editing bacterial...CRISPRi Cas9 gRNA plasmids from the Qi lab CRISPR...