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Showing: 21 - 31 of 31 results

  1. CRISPR Plasmids - Drosophila

    Type
    Collection
    ... Targeting RNA Editing Other Applications Purify Tag Visualize dCas9-FokI Screen Pooled Libraries gRNAs...and Port pAc-sgRNA-Cas9 dU6 BspQI Transfection Puromycin yes, cut Ji-Long Liu U6-BbsI-crRNA dU6 BbsI Transfection...

  2. CRISPR Plasmids - Prime Edit

    Type
    Collection
    ... Targeting RNA Editing Other Applications Purify Tag Visualize dCas9-FokI Screen Pooled Libraries gRNAs...-Puro Mammalian, piggyBac hU6 pegRNA BsmBI No Puromycin Jacob Giehm Mikkelsen pPBT-PE2-PuroTK-pegRNA_GG...

  3. CRISPR Plasmids - Plants

    Type
    Collection
    ... Targeting RNA Editing Other Applications Purify Tag Visualize dCas9-FokI Screen Pooled Libraries gRNAs...1 none As Cpf1 Qi pKEE401 yes, cut S. pyogenes Neomycin Chen Do you have suggestions for other plasmids...

  4. CRISPR Plasmids - Empty gRNA Vectors

    Type
    Collection
    ... Targeting RNA Editing Other Applications Purify Tag Visualize dCas9-FokI Screen Pooled Libraries gRNAs...pyogenes URA3 Wyrick pCRISPomyces-2 61737 Bacteria BbsI yes, cut S. pyogenes Apramycin Zhao pRB1017 59936...none S. pyogenes Zon pCRISPomyces-1 61736 Bacteria BbsI yes, cut S. pyogenes Apramycin Zhao pMB70 47943 Worm...pyogenes tagRFP Ebert pL-CRISPR.SFFV.tRFP 57826 Mammalian/Lentiviral BsmBI yes, cut S. pyogenes tagRFP Ebert...activate, interfere, or nick. Selection , such as Puromycin or EGFP Cloning enzyme used for insertion of your...Mammalian/Lentiviral BbsI none S. pyogenes Puro, TagBFP Yusa lentiGuide-Puro 52963 Mammalian/Lentiviral...Mammalian/Lentiviral BsmBI yes, cut S. pyogenes tagRFP Ebert pFGC-pcoCas9 52256 Plant AscI, PacI, SbfI...

  5. Lentiviral Prep Service

    Type
    Collection
    ...carrying version 1 of the CellTag barcoding library. Version 1 of the Celltag library contains 19973 barcodes...carrying version 2 of the CellTag barcoding library. Version 2 of the Celltag library contains 4934 barcodes...carrying version 3 of the CellTag barcoding library. Version 3 of the Celltag library contains 5737 barcodes... genes along with 1000 non-targeting controls Puromycin Doench , Root 73179 Human gRNA pooled library ... genes along with 1000 non-targeting controls Puromycin Doench , Root Human activation pooled libraries...56,762 unique sgRNAs targeting 18,885 human genes Puromycin Doench , Root Human inactivation pooled libraries...human genes along with 500 non-targeting controls Puromycin Doench , Root 92386 Human CRISPR inhibition library...

  6. Lentivirus Plasmids

    Type
    Collection
    ... genome, it has the potential for insertional mutagensis. The 2nd and 3rd generation lentiviral systems...-pLKO-puro 3rd inducible expression of shRNA; puromycin selection. See plasmid 21916 for Neo selection...cDNA expression, gateway plasmid, constitutive Hygromycin resistance gene coexpression. See article for...19319 pLJM1-EGFP 3rd for EGFP fusion; PGK driven puromycin Sabatini 25895 pLX301 3rd Gateway plasmid for ...Tet-pLKO-neo 3rd inducible expression of shRNA; neomycin selection. See plasmid 21915 for Puro resistance...

  7. Retrovirus Plasmids

    Type
    Collection
    ...results in long-term transgene expression. Some disadvantages of γ-retroviruses are that they can only transduce... can lead to oncogenesis (termed insertional mutagenesis). Read our γ-Retrovirus Guide for more information...pBABE-puro MoMLV For transgene expression, with Puromycin selection (for additional pBabe plasmids, browse... For cloning and gene expression; select with puromycin or screen for GFP Bartel 32702 pMSCV-loxp-dsRed-loxp-eGFP-Puro-WPRE...

  8. Genomic Deletions in Mammalian Cell Lines

    Type
    Collection
    ...joining (NHEJ) repair. Deletions have potential advantages as compared to single-site small indels given...Addgene plasmid ID 48139), which include GFP and puromycin as selectable markers, respectively, or constructs...consists of DMEM supplemented with 2% penicillin/ streptomycin and 1% L-glutamine, which is used for MEL cells...

  9. Penn Vector Core Partnership with Addgene

    Type
    Collection
    ...Loren Looger AV-1-PV3661 98927-AAV1 pENN.AAV.CAG.Flex.GFPsm_myc.WPRE.SV40 Loren Looger AV-10-PV0101 105530...James M. Wilson AV-9-PV2453 45185-AAV9 AAV-EF1a-BbTagBY Joshua Sanes AV-9-PV2454 45186-AAV9 AAV-EF1a-BbChT...Boyden AV-1-PV3511 98926-AAV1 pAAV.CAG.GFPsm-myc.WPRE.SV40 Loren Looger AV-1-ALL856 100048-AAV1 pAAV.CAG.LSL.tdTomato...

  10. TALENs for Endogenous Zebrafish Genes

    Type
    Collection
    ...apoeb TAL3402 & TAL3403 TGCCAGGCTCGTAGCCTGttccaggctgatgccccTCAGCCCAGATGGGAGGA arap3a TAL3026 & TAL3027...ARSA TAL 3002 & TAL 3003 TCAAGAGGCGGTCTCCCTctcaacgagaccaccaTAGCCGAGGTGCTGAAGA asun TAL3404 & TAL3405 TAACTCAAGCCAACTCCAggaaacatcaggagtttGCAGGAAGATTGAGTTCA...cited2 TAL3228 & TAL3229 TGGTAGACCGCATGATGGcaatgaaccatggtcgTTTCCCAGATGCTGTGAA cited4a TAL3046 & TAL3047...clcn1b TAL3052 & TAL3053 TTTTATTGTGTTACAGACtgcctagcccgtgtccAGAGGTATATTGTGACAA col22A1 TAL3410 & TAL3411...crispld2 TAL3232 & TAL3233 TATCAGGAGGAGCTAGAAcccaacagcactaaacCCGACGCTCCTGTCCGAA cx52.6 TAL3056 & TAL3057...CYP20A1 TAL3414 & TAL3415 TAGACTTTCCTGAAAAGCaaaaatgctagattttGCCATTTTTGCTGTGACA dachc TAL3234 & TAL3235 ...TCCCGTGCAAGGAATGAAtgcaaaaacatttcctTTCCCTTGTTTTGACGGA dcps TAL3416 & TAL3417 TGAACAGTCCACCGATAAcccagtggtagacttcCAGCTCACCCGCATTCTA ddx26b TAL3418 & TAL3419...

  11. Ras Pathway

    Type
    Collection
    ...protein, LST8 homolog MTOR Mechanistic target of rapamycin NF1 Neurofibromin 1 NFE2L2 Nuclear factor, erythroid...SPRY1 SPRY2 SPRY3 SPRY4 Sprouty RTK signaling antagonist STK STK3 STK4 Serine/threonine kinase STK11 Serine...
Showing: 21 - 31 of 31 results