...and Myers Tagging Plasmids Protein Species Tag Donor Plasmid gRNA plasmid gRNA plasmid FLAG pFETCh_Donor... Tagging Plasmids: pX330S-2-PITCh - expresses Cas9 & PITCh-gRNA pX330A-FBL/PITCh - expresses Cas9, PITCh-gRNA...Regulation Activate Interfere Epigenetics RNA Targeting RNA Targeting RNA Editing Other Applications Purify Tag...reaction produces a single plasmid targeting vector that encodes both a guide RNA transcript and a repair...the remaining 5 plasmids include Cre and FLP expression vectors, a general cloning plasmid, and a prebuilt...PX458 . Multiple gRNA plasmids may be used. The HDR (homology directed repair) donor plasmid with homology..., which expresses an untagged Cas9 AND a gRNA targeting the AAVS1 locus. Doyon Tagging Plasmid: 3xFLAG...

...Marker PI Publication Empty gRNA Expression Vectors Select a gRNA expression plasmid based on factors such ...Regulation Activate Interfere Epigenetics RNA Targeting RNA Targeting RNA Editing Other Applications Purify Tag...variety of Cas-containing plasmids. ID Plasmid Promoter Cloning Enzyme Co-expressed Cas9 Cas9 System Selection...base-to-base conversions using a prime editing guide RNA (pegRNA). The pegRNA directs the nickase to the target site...If the plasmid that you choose does not also express a gRNA, you will need to use a separate gRNA expression...If the plasmid you’re using does not also express a gRNA, you will need to use a separate gRNA expression...CRISPR Guide Viral Preps Protocols gRNA Design Tools CRISPR Blog Posts The following CRISPR plasmids have...

...Regulation Activate Interfere Epigenetics RNA Targeting RNA Targeting RNA Editing Other Applications Purify Tag...CRISPR expression plasmids that have been designed for use in parasites. CRISPR...suggestions for other plasmids that should be added to this list? Fill out our Suggest a Plasmid form or e-mail...Zebrafish Xenopus CRISPR Resources CRISPR Guide Viral Preps Protocols gRNA Design Tools CRISPR Blog Posts Eukaryotic... CRISPR genome-wide knockout gRNA pooled library designed with 10 guides against 8,158 predicted protein-coding...Plasmids: Parasites Browse CRISPR Plasmids By Function Genome Editing Cut Base Edit Nick Prime Edit Transcriptional...development. This collection brings together some of the plasmids that have been used in these efforts. The content...

...Cas proteins then express and process the CRISPR loci to generate CRISPR RNAs (crRNAs). Through sequence...guanosine. RNA editors : Cas13 fused to the ADAR2 deaminase convert adenosine to inosine on RNA. Inosine...check out Addgene’s CRISPR Guide and CRISPR blog posts , or find CRISPR plasmids for your research. Addgene...CRISPR-Cas nuclease specificity using truncated guide RNAs. Nat Biotechnol . 32(3):279-84. PMID: 24463574...Weissman JS, Qi LS. 2013. CRISPR-mediated modular RNA-guided regulation of transcription in eukaryotes. Cell... JA, Charpentier E. 2012. A programmable dual-RNA-guided DNA endonuclease in adaptive bacterial immunity... M, DiCarlo JE, Norville JE, Church GM. 2013. RNA-guided human genome engineering via Cas9. Science . ...

...structure/gene expression. RNA Targeting RNA Targeting Type VI CRISPR systems target RNA, not DNA, across...select CRISPR lentiviral plasmids. CRISPR Plasmid Kits : Find groups of plasmids that are used together ...resources or browse plasmids below by function. Addgene's CRISPR collections feature plasmids for a variety ...multiple systems. RNA Editing Cas13 enzymes fused to deaminases can edit bases in RNA. Other Applications...experiment based on a variety of factors. gRNAs gRNA plasmids to target specific genes....CRISPR plasmids available at Addgene. Search by species and CRISPR function. CRISPR... CRISPR Plasmids and Resources...

...Jeffrey Essner Lab CRISPR-Cas/RNA-Guided Nuclease (RGN) expression plasmids - Keith Joung Lab CRISPR/Cas9... Plasmid Collections Zebrafish Plasmids Zebrafish Plasmids and Resources... of the plasmids and tools available at Addgene for use in zebrafish. Browse Plasmids Plasmid Tools for...biosensors, and Cre expression plasmids. Large collections or kits are described below . ID Plasmid Description...resources for a full selection of plasmids expressing Cas9 and empty gRNA backbones. A few examples of useful...fusion expression in organisms or cells in which an exogenous mRNA can be injected and expressed. EXPRESs...for plasmids containing your zebrafish gene of interest? Search Addgene's collection for plasmids that...

...CRISPR RNA Targeting CRISPR Plasmids: RNA Targeting Browse CRISPR Plasmids By Function Genome Editing Cut ...tables below for CRISPR RNA targeting plasmids. Plasmids are available for expression in mammalian systems...Regulation Activate Interfere Epigenetics RNA Targeting RNA Targeting RNA Editing Other Applications Purify Tag...CRISPR plasmids for targeting RNA sequences, which include the enzymes C2c2/Cas13a and Cas13b. CRISPR... target RNA rather than DNA. In bacteria, once they have recognized and cleaved the target RNA sequence...to attentuate RNA levels. In mammalian systems, Cas13a does not exhibit the collateral RNA degradation ...Mammalian ID Plasmid Gene/Insert Promoter Selectable Marker PI Publication Bacterial ID Plasmid Gene/Insert...

... (2013). Plasmid is low copy number. 45946 pU6-BbsI-chiRNA : Plasmid for expression of chiRNA under the...Lab CRISPR Plasmids flyCRISPR Plasmids Available from Addgene You may also like... CRISPR Guide CRISPR Protocols.... These plasmids are described in: Genome engineering of Drosophila with the CRISPR RNA-guided Cas9 nuclease...window) Article Individual plasmids can be ordered via the links below: ID Plasmid 46294 pBS-Hsp70-Cas9 : ...Browse CRISPR Cas9 gRNA plasmids for drosophila research from the O'Connor-Giles, Wildonger, and Harrison...generated within a month. phsp70-Cas9 and a single chiRNA plasmid can be coinjected to generate mutations via...DSBs. Coinjection of phsp70-Cas9 and plasmids encoding two chiRNAs can induce defined deletions between...

...Promoter PI Publication Empty gRNA Expression Vectors Select a gRNA expression plasmid based on factors such ...Cas-containing plasmids. ID gRNA Plasmid Promoter Cloning Enzyme(s) Delivery Resistance Co-expressed Cas9 Depositing...Regulation Activate Interfere Epigenetics RNA Targeting RNA Targeting RNA Editing Other Applications Purify Tag...If the plasmid that you choose does not also express a gRNA, you will need to use a separate gRNA expression...CRISPR Guide Viral Preps Protocols gRNA Design Tools CRISPR Blog Posts The following CRISPR plasmids have..., nick, etc.) On the other hand, gRNA plasmids that do not co-express a Cas protein can be paired with...Cas9 plasmid Joung 46170 PU6::klp-12_sgRNA cU6 PCR (see paper) Transfection none, need Cas9 plasmid Calarco...

...Regulation Activate Interfere Epigenetics RNA Targeting RNA Targeting RNA Editing Other Applications Purify Tag...below for CRISPR plasmids to help purify genomic loci. Plasmids are available for expression in mammalian ...Mammalian ID Plasmid Gene/Insert Promoter Selectable Marker PI Publication Bacteria ID Plasmid Gene/Insert...suggestions for other plasmids that should be added to this list? Fill out our Suggest a Plasmid form or e-mail...Zebrafish Xenopus CRISPR Resources CRISPR Guide Viral Preps Protocols gRNA Design Tools CRISPR Blog Posts Identifying...Catalytically inactive CRISPR plasmids for purification of target loci. CRISPR...Purify Genomic Loci Browse CRISPR Plasmids By Function Genome Editing Cut Base Edit Nick Prime Edit Transcriptional...

...Reporter Plasmids You may also like… Plasmids 101 Addgene's Molecular Biology Guides Plasmids 101: E. ...CRISPR plasmids, see here . pgRNA-bacteria 44251 CRISPR Stanley Qi Expression of customizable guide RNA ...Controlled Expression Reporter Plasmids Reporter Plasmids Tagging and Visualization Purification Controlled Expression...or staining, and RNA transcript tags to monitor RNA localization. Using these plasmids you’ll hopefully... on expression only at a specific time. This collection has a variety of plasmids whose expression levels...interest. Plasmid ID Promoter Inducer(s) PI Purpose Murray Lab pBEST plasmids Various Plasmids pBAD, OR2...drive gene expression at a specific level. Return to Top Reporter Plasmids Reporter plasmids can be used...

...γ-Retroviral Plasmids γ-Retroviral Plasmids Additional Resources: γ-Retrovirus Guide Biosafety Guide Lentiviral...10676 pMKO.1 GFP MoMLV U6-driven plasmid for shRNA expression; also expresses GFP. See pMKO.1 puro for Puro...retroviral plasmid Vignali 27995 TtRMPVIR CMV/MSV Tet-regulated expression of shRNA; expresses rtTA. See... additional plasmids in this toolkit. Lowe 64865 pLncEXP CMV/MSV lncRNA expression plasmid Sun 60683 pLXIN-Luc...Lentiviral Plasmids AAV Plasmids Viral Vectors 101 eBook γ-Retrovirus (gamma-retrovirus) is an RNA virus comprised... and Packaging Plasmids Common γ-retroviral envelope and packaging plasmids. ID Plasmid Type Description...with packaging plasmid Weinberg 12259 pMD2.G Envelope VSV-G-expressing envelope plasmid Trono 1733 pCI-VSVG...

...Cas-containing plasmids. ID gRNA Plasmid Promoter Cloning Enzyme(s) Validated In Resistance Co-expressed Cas9 Depositing...Regulation Activate Interfere Epigenetics RNA Targeting RNA Targeting RNA Editing Other Applications Purify Tag...If the plasmid that you choose does not also express a gRNA, you will need to use a separate gRNA expression...If the plasmid you’re using does not also express a gRNA, you will need to use a separate gRNA expression...CRISPR Guide Viral Preps Protocols gRNA Design Tools CRISPR Blog Posts The following CRISPR plasmids have...sites. ID Plasmid Gene/Insert Promoter Selectable Marker PI Publication Empty gRNA Expression Vectors Select... Select a gRNA expression plasmid based on factors such as selectable marker or cloning method. When ...

...Regulation Activate Interfere Epigenetics RNA Targeting RNA Targeting RNA Editing Other Applications Purify Tag...tables below for CRISPR-FokI plasmids. Plasmids are available for expression in mammalian systems and Drosophila...Drosophila. Mammalian ID Plasmid Gene/Insert Promoter PI Publication Drosophila ID Plasmid Gene/Insert Promoter...Yeast ID Plasmid Gene/Insert Promoter PI Publication Do you have suggestions for other plasmids that should...Zebrafish Xenopus CRISPR Resources CRISPR Guide Viral Preps Protocols gRNA Design Tools CRISPR Blog Posts A catalytically...CRISPR plasmids with dCas9 fused to FokI for creating double-strand breaks (DSBs). CRISPR...FokI CRISPR: dCas9-FokI Browse CRISPR Plasmids By Function Genome Editing Cut Base Edit Nick Prime Edit Transcriptional...

...Regulation Activate Interfere Epigenetics RNA Targeting RNA Targeting RNA Editing Other Applications Purify Tag...below for CRISPR Cascade-Cas3 plasmids. Plasmids are available for expression in mammalian systems, bacteria...the Cascade complex to express equally. To combat this, some CRISPR-Cas3 plasmids take an all-in-one approach...plants. Mammalian ID Plasmid Gene/Insert Promoter PI Publication Bacteria ID Plasmid Gene/Insert Promoter...suggestions for other plasmids that should be added to this list? Fill out our Suggest a Plasmid form or e-mail...Zebrafish Xenopus CRISPR Resources CRISPR Guide Viral Preps Protocols gRNA Design Tools CRISPR Blog Posts Cas3...Find plasmids for CRISPR-Cas and Cascade for large genomic deletions. CRISPR...

...Addgene plasmid ID 42230). This plasmid allows for the simultaneous expression of sgRNA and SpCas9, but... multiple sgRNAs may be expressed from a single plasmid. Add “CACC” before the 20-mer guide sequence and...cellular delivery of a pair of chimeric single guide RNAs (sgRNAs) to create two double strand breaks (DSBs...Cell Lines Other Addgene Resources CRISPR Plasmids CRISPR Guide Addgene Blog Posts Addgene is proud to present...cloning and expression purposes. NOTE: Here we discuss the usage of the pSpCas9(BB) plasmid (pX330) (Addgene...utilized, such as pX458 (Addgene plasmid ID 48138) or pX459 (Addgene plasmid ID 48139), which include GFP ... with a PCR cloning kit) into a plasmid vector. Transform the plasmid into DH5α E. coli cells and plate...

...blog . Eukaryotic Pathogen CRISPR guide RNA/DNA Design Tool (EuPaGDT) gRNA design tool with extensive selection... CRISPR plasmid summary for transcriptional activation dCas9 activators sgRNA dual expression: pAC2 , ....9 KB Sontheimer gRNA design and cloning All-in-one plasmid containing expression cassette for NmCas9 ...who have deposited CRISPR plasmids , we've created a series of links and guides to help you use CRISPR in...your input sequence or list of sequences. RGEN (RNA-guided endonuclease) Tools: Cas-OFFinder & Cas-Designer...) Description Plasmids in protocol Download protocol Church gRNA design and cloning gRNA cloning vector...583.3 KB Zhang gRNA cloning CRISPR RNA array: Cas9 (pX260) or Cas9 D10A (pX334) ; tracrRNA: Cas9 (pX330)...

... protocols for expression in the Open Bioeconomy Lab's crowd-sourced expression guides (Link opens in ...Return to top RNA Polymerases Addgene ID Plasmid Gene/insert 165505 pOpen-T7gene1 T7 RNA Polymerase 165516...Bacterial Protein Expression Vectors BioBricks Foundation Plasmids Drew Endy Lab Plasmids Jennifer Molloy...enzymes into an expression vector of their choice and then transform into E. coli for expression and purification...Golden Gate assembly. Plasmids Explore the collection and order individual plasmids via the links below:...Reverse Transcriptases RNA Polymerases Other Enzymes DNA Ligases Addgene ID Plasmid Gene/insert 165503 pOpen-HSlig4...165516 pOpen-SP6rpol SP6 RNA Polymerase 165576 pOpen-T3gene1 T3 RNA Polymerase Return to top Other Enzymes...

...Regulation Activate Interfere Epigenetics RNA Targeting RNA Targeting RNA Editing Other Applications Purify Tag...tables below for CRISPR nickase plasmids. Plasmids are available for expression in mammalian systems, bacteria...Mammalian ID Plasmid Gene/Insert Promoter Selectable Marker PI Publication Bacteria ID Plasmid Gene/Insert...Drosophila ID Plasmid Gene/Insert Promoter Selectable Marker PI Publication Plant ID Plasmid Gene/Insert...suggestions for other plasmids that should be added to this list? Fill out our Suggest a Plasmid form or e-mail...Zebrafish Xenopus CRISPR Resources CRISPR Guide Viral Preps Protocols gRNA Design Tools CRISPR Blog Posts CRISPR...CRISPR nickase plasmids for the generation of single-strand breaks. CRISPR...

...Regulation Activate Interfere Epigenetics RNA Targeting RNA Targeting RNA Editing Other Applications Purify Tag...below for CRISPR base editing plasmids. Plasmids are available for expression in mammalian systems, bacteria...CRISPR Plasmids: Base Edit Browse CRISPR Plasmids By Function Genome Editing Cut Base Edit Nick Prime Edit...Mammalian ID Plasmid Gene/Insert Promoter Selectable Marker PI Publication Bacteria ID Plasmid Gene/Insert...Publication Plant ID Plasmid Gene/Insert Promoter Selectable Marker PI Publication Yeast ID Plasmid Gene/Insert...suggestions for other plasmids that should be added to this list? Fill out our Suggest a Plasmid form or e-mail...Zebrafish Xenopus CRISPR Resources CRISPR Guide Viral Preps Protocols gRNA Design Tools CRISPR Blog Posts Two...