... pU6-sgGFP-NT1 Human pSico-based U6 vector containing murine U6 promoter and sgRNA targeting GFP (NT1)...pSLQ1658-dCas9-EGFP Human expression vector containing dCas9 that is fused to 2x NLS and EGFP for CRISPR ...targeting endogenous CD71 gene 46919 pMLS-SV40-EGFP Target EGFP gene that is stably integrated into HEK293...CRISPR imaging 51024 pSLQ1651-sgTelomere(F+E) Lentiviral vector with sgRNA targeting human telomeres 46913 ...dCas9 fused to N 51025 pSLQ1661-sgMUC4-E3(F+E) Lentiviral vector that contains sgRNA targeting repetitive...

...Cre-dependent expression of diphtheria toxin receptor (DTR)–GFP fusion protein. Azim et al. Nature. 2014 Apr 17. ... Proc Natl Acad Sci U S A. 2023 Aug 22. Use a lentiviral FRET-based reporter to study tau seeding in mammalian...

...fluorescent marker like green fluorescent protein (GFP), creating a customizable DNA or RNA label for fluorescence...CRISPR libraries What Are Pooled Lentiviral CRISPR Libraries? Pooled lentiviral CRISPR libraries are a heterogeneous...need to generate lentivirus containing the entire library (Figure 8D). This lentivirus will then be used...genome-wide screens using CRISPR involves pooled lentiviral CRISPR libraries. Figure 8: Overview of genome-wide...heterogeneous population of lentiviral transfer vectors. In most cases, each plasmid contains an individual...Figure 8A), then cloned in a pooled format into lentiviral transfer vectors (Figure 8B). Libraries come ...library is suitable for your experiment. Although lentiviral libraries are the most popular method for CRISPR...

...has efficient ver. 2 structure. 92220 pLenti_dCas9-2xAM Lentiviral plasmid expressing dCas9-2xAM and gRNA...gRNA cloned in Bbs I sites. 92221 pLenti_dCas9-2xAM_hIRF-1 Lentiviral plasmid expressing dCas9-2xAM and...genomic regions of interest 82613 3xFLAG-dCas9/MSCV-EGFP Expresses 3xFLAG-dCas9 in mammalian cells for enChIP...